For better understanding watch the previous video about SDS-PAGE, you can find the video in the following link: • The principle of SDS P...
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@hadiahd11673 жыл бұрын
what i couldnt understand in a full week i got it in 10 minutes, god bless you
@zehrayilmaz7654 Жыл бұрын
i ve just discovered this channel and its amazing
@simpleaka.x634 жыл бұрын
Voice is calming, The teaching is clear. thanks alot
@AngieBedoya175 жыл бұрын
I loved this video. Thank you so much !! Eres la mejor :D
@ahaskarkarde41637 жыл бұрын
Thank u ma'am for such a wonderful video.very educational and clear
@biomedicalandbiologicalsci49897 жыл бұрын
Thank you for your comment .. stay tuned ;)
@ruchinambiar80053 жыл бұрын
This video was very helpful. Please continue posting such videos. You are a great help
@maryamgeraeili84214 жыл бұрын
wonderful. without this video I could not understand it. Thank you very much
@smithabb6723 Жыл бұрын
Please make more videos. I love your explanation. It's very thorough 👍🏼
@francescasola61145 жыл бұрын
This video is clear and very very useful. Thank you!
@leticiaalvescavalcante95937 жыл бұрын
Why haven't this video more views or comments?! I already sad "thank you" in your video about SDS-Page, but I'll say again: THANK YOU SO MUCH! Please don't stop making videos
@biomedicalandbiologicalsci49897 жыл бұрын
Thank youu for the encouragement, I will not stop :)
@umairsaab14035 жыл бұрын
Hi
@user-px5si6po5l2 жыл бұрын
Very clear video for beginners
@fatnmchollandl65055 жыл бұрын
Thank you, your video are extremely helpful. Please don't stop making them.
@Nickel45 жыл бұрын
Really really thank you so much! Your videos makes everything so simple, they're so useful :) ❤️
@Boooommerang4 жыл бұрын
Excellent video! Congratulations
@safaeelarrafe79635 жыл бұрын
your accent is very clear and really thanks for your explanation
@hfyyshnur_3 жыл бұрын
thank you ! really helpful during this pandemic situation.
@feruzarakhimova1052 жыл бұрын
It was very helpful. Thank you very much!!!
@hlayisekamkharishikwambana59995 жыл бұрын
you are the best
@gregorychileshe59552 жыл бұрын
Excellent explanation. Thanks
@lyssc995 жыл бұрын
this should have more views. thank u for putting this up it was really helpful!!!!! :-)
@azzaabdulaziz91795 жыл бұрын
Thanks for the video; what is the Conc. of the SDS PAGE gel, and is it only resolving without stacking? is there any buffer added with sample during separation of the PI step?
@arnabmandal94994 жыл бұрын
Do you have any lecture on Mass spectroscopy??please let me know!! And if not then a humble request to you make lecture on that topic please !!! And its a best lecture on this particular topic which is really helpful to understand !!!Thank you so much Mam!!👏👏👏🙏
@learntechp70306 жыл бұрын
Extraordinaryly explained madam thank u so much
@JazzyJADAxxx Жыл бұрын
Thanks doc 💗
@nadiahappy4107 жыл бұрын
well done! it is very useful
@subhankardas84773 жыл бұрын
Very nicely explained ❤️
@apoorvaverma41694 жыл бұрын
Maa'm after the 1D sepration of proteins ..they were at their isoelectric point ...so had zero chagre But in second sepration i.e. SDSsepration u said proteins move from negative to positive electrode If they didnt had any charge on them ....how are proteins moving For sds we need charge on proteins to move in electrical feild Please explain this
@abhisaini93353 жыл бұрын
👍👍👍
@mairapradobailey9793 Жыл бұрын
thank you!
@lilithasotondoshe82264 жыл бұрын
you are so clear!
@veliborcabarkapa42715 жыл бұрын
Ver, very useful!!!! Thank you!
@janamitra13825 жыл бұрын
After performing IEF the proteins will become neutral, will there be movement after applying SDS which obtains -ve charge due to the presence of ampholytes.
@sarahgamal55725 жыл бұрын
thank you so much it's very useful
@d.r-monaelhuseiny41553 жыл бұрын
Thank you
@snahasars6 жыл бұрын
Hello, I wanted to ask can the SDS PAGE be performed prior to the IEF? Why do we need to maintain the order of IEF and then SDS-PAGE? Thank you!
@biomedicalandbiologicalsci49896 жыл бұрын
Hello, no actually you cannot perform SDS before you should keep the same order .. The idea is to separate proteins who have the same MW ... if you perform SDS first then you have your proteins in the gel ... so you need a special container to put the gel in and to apply the PH gradient ... but in this case, you perform the IEF when the proteins are in the liquid and then you apply the strip directly to the gel ...
@amonoracheal91185 жыл бұрын
Great explanation! thank you
@biology92145 жыл бұрын
Soo good ...but mam plzz.speak english like india for easy to listening for indian
@sampakundu81296 жыл бұрын
Thanku mam for this nice vedio.Mam please says about denatured and non denatured 2D gel.
@kaustubhbhartiya65215 жыл бұрын
Thanks a lot madam
@sesd46626 жыл бұрын
Thanks doctor this is amazing
@smart99246 жыл бұрын
Mam please upload for immuno gel electrophoresis....i understood only because of this. Thank you so much
@shabbirshabbani20194 жыл бұрын
at ph =3 what will be the emergences order of the amino acid in column on the basis of defferent pka value ?
@kajalkabdwal18155 жыл бұрын
Mam what if the two proteins have same isoelectric point and molecular weight??
@EilafBadr--5 жыл бұрын
Thank you so much
@amonoracheal91185 жыл бұрын
However, I guess the example of the second dimension of separating proteins of different PIs yet with the same molecular weight had a mistake in the figures:)
@victoranolu43764 жыл бұрын
I am taking a course in biochemistry titled biochemical reasoning. Do you have any material on it?
@madhumithaayyappan52775 жыл бұрын
THANK U
@geetarani28755 жыл бұрын
v good..
@warshafernando7273 жыл бұрын
Thank you❤️
@mfkmina40325 жыл бұрын
where can I find articls which use this method!?
@Mohitthakurofficial7 жыл бұрын
Madam when is next video coming? I asked for Blotting techniques and Chromatographic techniques.
@biomedicalandbiologicalsci49897 жыл бұрын
Hello, actually I am working at the moment on a huge topic which is the CRISPR-Cas9 technique, which needed tow videos to be prepared, I will upload the videos soon and then the next will be blotting technique, and then I will work on chromatography because it might also need tow videos. stay around my friend, a lot of interesting videos are coming up ;)
@Mohitthakurofficial7 жыл бұрын
Thanks, I'm eagerly waiting for the videos to come. :)
@biomedicalandbiologicalsci49897 жыл бұрын
Hey ... Here are the two videos about blotting techniques, the first is about western blotting and the second is about southern blotting and northern blotting: kzfaq.info/get/bejne/eM2lgayBzqnRpmw.html kzfaq.info?o=U&video_id=RvbzjYM_Ok0 I hope you will enjoy them :)
@biomedicalandbiologicalsci49897 жыл бұрын
Really ... can u please tell me about the mistake!!! In which video is it ... the agarose gel electrophoresis???
@nepaleseanuragsharmaoffici52027 жыл бұрын
plz mam can you post about pulsed field gel electrophoresis and native too coz i hav got exams soon bt i didnt get it😐😐
@biomedicalandbiologicalsci49897 жыл бұрын
I will try to make it .. but tell me .. when is your exam ??
@biomedicalandbiologicalsci49897 жыл бұрын
Hei, a bit late but here you are, the link for the pulsed field electrophoresis video: kzfaq.info/get/bejne/lcWBm6aJlpjMaH0.html
@ajendrayadav79477 жыл бұрын
when we load the protein in the well ,then how it is seprate to its ph
@biomedicalandbiologicalsci49897 жыл бұрын
In the first step, and to separate the proteins according to their PH we dont apply then in a well, we apply the proteins mixture in the container where there is a strip containing a PH gradient, and then the proteins will be separated according to their isoelectric point (see from 7.34 min)
@shilpapadmanaban14206 жыл бұрын
So how do we denature the proteins before loading it onto to the SDS page gel? Do we just flood the ph strip with loading dye?
@ajendrayadav79477 жыл бұрын
we know the ph of protein before loading
@biomedicalandbiologicalsci49897 жыл бұрын
Normally, we know the isoelectric points of the protein of interest ...
@shjalalshah41874 жыл бұрын
If you please also upload the ppt files that will be great
@Bashariat15 жыл бұрын
thanks for the explanation. but 15 min is too long