The principle of 2D Gel Electrophoresis/and the isoelectric point

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The principle of 2D Gel Electrophoresis/and the isoelectric point

Рет қаралды 74,906

Күн бұрын

For better understanding watch the previous video about SDS-PAGE, you can find the video in the following link:
• The principle of SDS P...

Пікірлер: 71

@hadiahd1167 3 жыл бұрын

what i couldnt understand in a full week i got it in 10 minutes, god bless you

@zehrayilmaz7654 Жыл бұрын

i ve just discovered this channel and its amazing

@simpleaka.x63 4 жыл бұрын

Voice is calming, The teaching is clear. thanks alot

@AngieBedoya17 5 жыл бұрын

I loved this video. Thank you so much !! Eres la mejor :D

@ahaskarkarde4163 7 жыл бұрын

Thank u ma'am for such a wonderful video.very educational and clear

@biomedicalandbiologicalsci4989 7 жыл бұрын

Thank you for your comment .. stay tuned ;)

@ruchinambiar8005 3 жыл бұрын

This video was very helpful. Please continue posting such videos. You are a great help

@maryamgeraeili8421 4 жыл бұрын

wonderful. without this video I could not understand it. Thank you very much

@smithabb6723 Жыл бұрын

Please make more videos. I love your explanation. It's very thorough 👍🏼

@francescasola6114 5 жыл бұрын

This video is clear and very very useful. Thank you!

@leticiaalvescavalcante9593 7 жыл бұрын

Why haven't this video more views or comments?! I already sad "thank you" in your video about SDS-Page, but I'll say again: THANK YOU SO MUCH! Please don't stop making videos

@biomedicalandbiologicalsci4989 7 жыл бұрын

Thank youu for the encouragement, I will not stop :)

@umairsaab1403 5 жыл бұрын

@user-px5si6po5l 2 жыл бұрын

Very clear video for beginners

@fatnmchollandl6505 5 жыл бұрын

Thank you, your video are extremely helpful. Please don't stop making them.

@Nickel4 5 жыл бұрын

Really really thank you so much! Your videos makes everything so simple, they're so useful :) ❤️

@Boooommerang 4 жыл бұрын

Excellent video! Congratulations

@safaeelarrafe7963 5 жыл бұрын

your accent is very clear and really thanks for your explanation

@hfyyshnur_ 3 жыл бұрын

thank you ! really helpful during this pandemic situation.

@feruzarakhimova105 2 жыл бұрын

It was very helpful. Thank you very much!!!

@hlayisekamkharishikwambana5999 5 жыл бұрын

you are the best

@gregorychileshe5955 2 жыл бұрын

Excellent explanation. Thanks

@lyssc99 5 жыл бұрын

this should have more views. thank u for putting this up it was really helpful!!!!! :-)

@azzaabdulaziz9179 5 жыл бұрын

Thanks for the video; what is the Conc. of the SDS PAGE gel, and is it only resolving without stacking? is there any buffer added with sample during separation of the PI step?

@arnabmandal9499 4 жыл бұрын

Do you have any lecture on Mass spectroscopy??please let me know!! And if not then a humble request to you make lecture on that topic please !!! And its a best lecture on this particular topic which is really helpful to understand !!!Thank you so much Mam!!👏👏👏🙏

@learntechp7030 6 жыл бұрын

Extraordinaryly explained madam thank u so much

@JazzyJADAxxx Жыл бұрын

Thanks doc 💗

@nadiahappy410 7 жыл бұрын

well done! it is very useful

@subhankardas8477 3 жыл бұрын

Very nicely explained ❤️

@apoorvaverma4169 4 жыл бұрын

Maa'm after the 1D sepration of proteins ..they were at their isoelectric point ...so had zero chagre But in second sepration i.e. SDSsepration u said proteins move from negative to positive electrode If they didnt had any charge on them ....how are proteins moving For sds we need charge on proteins to move in electrical feild Please explain this

@abhisaini9335 3 жыл бұрын

👍👍👍

@mairapradobailey9793 Жыл бұрын

thank you!

@lilithasotondoshe8226 4 жыл бұрын

you are so clear!

@veliborcabarkapa4271 5 жыл бұрын

Ver, very useful!!!! Thank you!

@janamitra1382 5 жыл бұрын

After performing IEF the proteins will become neutral, will there be movement after applying SDS which obtains -ve charge due to the presence of ampholytes.

@sarahgamal5572 5 жыл бұрын

thank you so much it's very useful

@d.r-monaelhuseiny4155 3 жыл бұрын

Thank you

@snahasars 6 жыл бұрын

Hello, I wanted to ask can the SDS PAGE be performed prior to the IEF? Why do we need to maintain the order of IEF and then SDS-PAGE? Thank you!

@biomedicalandbiologicalsci4989 6 жыл бұрын

Hello, no actually you cannot perform SDS before you should keep the same order .. The idea is to separate proteins who have the same MW ... if you perform SDS first then you have your proteins in the gel ... so you need a special container to put the gel in and to apply the PH gradient ... but in this case, you perform the IEF when the proteins are in the liquid and then you apply the strip directly to the gel ...

@amonoracheal9118 5 жыл бұрын

Great explanation! thank you

@biology9214 5 жыл бұрын

Soo good ...but mam plzz.speak english like india for easy to listening for indian

@sampakundu8129 6 жыл бұрын

Thanku mam for this nice vedio.Mam please says about denatured and non denatured 2D gel.

@kaustubhbhartiya6521 5 жыл бұрын

Thanks a lot madam

@sesd4662 6 жыл бұрын

Thanks doctor this is amazing

@smart9924 6 жыл бұрын

Mam please upload for immuno gel electrophoresis....i understood only because of this. Thank you so much

@shabbirshabbani2019 4 жыл бұрын

at ph =3 what will be the emergences order of the amino acid in column on the basis of defferent pka value ?

@kajalkabdwal1815 5 жыл бұрын

Mam what if the two proteins have same isoelectric point and molecular weight??

@EilafBadr-- 5 жыл бұрын

Thank you so much

@amonoracheal9118 5 жыл бұрын

However, I guess the example of the second dimension of separating proteins of different PIs yet with the same molecular weight had a mistake in the figures:)

@victoranolu4376 4 жыл бұрын

I am taking a course in biochemistry titled biochemical reasoning. Do you have any material on it?

@madhumithaayyappan5277 5 жыл бұрын

THANK U

@geetarani2875 5 жыл бұрын

v good..

@warshafernando727 3 жыл бұрын

Thank you❤️

@mfkmina4032 5 жыл бұрын

where can I find articls which use this method!?

@Mohitthakurofficial 7 жыл бұрын

Madam when is next video coming? I asked for Blotting techniques and Chromatographic techniques.

@biomedicalandbiologicalsci4989 7 жыл бұрын

Hello, actually I am working at the moment on a huge topic which is the CRISPR-Cas9 technique, which needed tow videos to be prepared, I will upload the videos soon and then the next will be blotting technique, and then I will work on chromatography because it might also need tow videos. stay around my friend, a lot of interesting videos are coming up ;)

@Mohitthakurofficial 7 жыл бұрын

Thanks, I'm eagerly waiting for the videos to come. :)

@biomedicalandbiologicalsci4989 7 жыл бұрын

Hey ... Here are the two videos about blotting techniques, the first is about western blotting and the second is about southern blotting and northern blotting: kzfaq.info/get/bejne/eM2lgayBzqnRpmw.html kzfaq.info?o=U&video_id=RvbzjYM_Ok0 I hope you will enjoy them :)

@biomedicalandbiologicalsci4989 7 жыл бұрын

Really ... can u please tell me about the mistake!!! In which video is it ... the agarose gel electrophoresis???

@nepaleseanuragsharmaoffici5202 7 жыл бұрын

plz mam can you post about pulsed field gel electrophoresis and native too coz i hav got exams soon bt i didnt get it😐😐

@biomedicalandbiologicalsci4989 7 жыл бұрын

I will try to make it .. but tell me .. when is your exam ??

@biomedicalandbiologicalsci4989 7 жыл бұрын

Hei, a bit late but here you are, the link for the pulsed field electrophoresis video: kzfaq.info/get/bejne/lcWBm6aJlpjMaH0.html

@ajendrayadav7947 7 жыл бұрын

when we load the protein in the well ,then how it is seprate to its ph

@biomedicalandbiologicalsci4989 7 жыл бұрын

In the first step, and to separate the proteins according to their PH we dont apply then in a well, we apply the proteins mixture in the container where there is a strip containing a PH gradient, and then the proteins will be separated according to their isoelectric point (see from 7.34 min)

@shilpapadmanaban1420 6 жыл бұрын

So how do we denature the proteins before loading it onto to the SDS page gel? Do we just flood the ph strip with loading dye?

@ajendrayadav7947 7 жыл бұрын

we know the ph of protein before loading

@biomedicalandbiologicalsci4989 7 жыл бұрын

Normally, we know the isoelectric points of the protein of interest ...