Why can't my professor just memorise this video and rap it during the lab. Very useful!
@biomedicalandbiologicalsci49897 жыл бұрын
Haha thank you ... maybe you can recommend it to your colleagues ;)
@MrPridizzle7 жыл бұрын
throw that ish on Rap Chat yo, that would be FIRE!
@julietten56146 жыл бұрын
your professor probs done thousands of 'gels' while doing his first research work. I m assuming he's just not interested any more:-))
@tartanhandbag6 жыл бұрын
this is why professors should do research and teachers should teach. not always the case; some professors are excellent, lively, dynamic and patient teachers also ...but most just want to get back to overseeing their projects, publishing papers and finishing grant applications before the dreaded deadlines.
@jimmyolum43443 жыл бұрын
Hehe
@gabrieldynkin50786 жыл бұрын
I just have to say it was the best explantion i've ever heard about anything! Thank you, it answered all of my questions....:)
@4MoonGlade46 жыл бұрын
Hello! This video really saved me! Detailed and exhaustive. I was studying this technique for an exam and didn't understand it really...now it's all clear. Thanks! 😊
@KJKP7 жыл бұрын
More detailed than other videos on SDS-PAGE.
@biomedicalandbiologicalsci49897 жыл бұрын
yup, stay tuned for more videos :)
@tedwills-topic95242 жыл бұрын
This helped me a lot before even doing my practicals. Thank you
@saniyapamnani84942 жыл бұрын
This is the best video I found on the internet about SDS PAGE. Thank you so much for all the details.
@brindabandas33412 жыл бұрын
This is the first technique video which I have completely understood without any practical experience. Thank you so much for making such a great video.
@ismailgbadamosi83214 жыл бұрын
This is the best video I have seen on the working principle of SDS PAGE. Thank you
@RevesAbsurdes16 жыл бұрын
Perfect ! I spend time looking for those informtion on internet and you give them (and a lot more) very clearly, good job !
@biomedicalandbiologicalsci49896 жыл бұрын
Hello, thank you for your comment ... happy you got what you need from the video :)
@RevesAbsurdes16 жыл бұрын
Biomedical and Biological Sciences yes i actually have an exam about all those technics soon and you saved my life haha !
@biomedicalandbiologicalsci49896 жыл бұрын
Wow, this is good ... I have a playlist on my channel containing videos about all the electrophoresis techniques, have a look at it, it will help you for your exam: kzfaq.info/get/bejne/hdSPjdKlycDJqH0.html
@julymgreenday19936 жыл бұрын
I finally undertood Electrophoresis!!!! THANK YOU! Greetings from Argentina ♥
@biomedicalandbiologicalsci49896 жыл бұрын
Hei, Glade you liked the video ... stay tuned :)
@medinlab1414 жыл бұрын
Thank you so much for the explanation, especially for the stacking gel's part! I found it very clear.
@bobmvideos4 жыл бұрын
OMG thanks, my wife has been doing these for like 25 years, now I know what the hell she has been talking about. Now to watch your video on westerns...
@swagpunjabiada65216 жыл бұрын
Mam please keep uploading such videos so that we can have clear concept about each technique ur way of teaching is great😄
@ranahaddad54462 жыл бұрын
I had watched many videos to understand this topic, but this video is definitely the best! Thank you so much!
@rogsang97642 жыл бұрын
thanks for the explanation, the best thing is that you dedicate time to explain the principle of the gel, its the best explanation i´ve seen so far :D
@leticiaalvescavalcante95937 жыл бұрын
OH MY GOD!!!! Thank you SO much!!! I'm a student from Brazil and I was looking for something that would make me understand the electrophoresis tecnique and I was not findind anything so simple and easy like this video! I'm so happy right now... I was almost giving up haha I was reading, and reading, and reading and not understanding at all, but now is everything so clear in my mind because of you...Omg, THANK YOUUUUU!!!!!!
@biomedicalandbiologicalsci49897 жыл бұрын
WOOW .. thank you for the nice comment ... stay tuned, you might find everything you are searching for in this channel :)
@sumantahalder10277 жыл бұрын
I am too pleased to watch your videos, these are very clear concept growing video. Thank you very much keep it up.
@biomedicalandbiologicalsci49897 жыл бұрын
Thank you for your comment ... I will ... Stay tuned :)
@justcat60426 жыл бұрын
This is the most helpful video i have ever watched about sds page. Now i understand much more about this! Thank you so much
@geralthasan2 жыл бұрын
you have my thanks madam for saving me from mid exam disaster. Thank you so much
@uzoechisamuel3 жыл бұрын
And somebody has been messing with my brain all this while. Anytime I see you in person, I will give you a bottle of win.
@murielmirus56414 жыл бұрын
thank you so much for the hard work you put into this video! it is very much appreciated :) your video has helped me a lot in the lab today!
@UQMD4 жыл бұрын
Thanks for the fantastic video! We just watched your video in class and it definitely helped us understand electrophoresis. I especially loved the pace of your video :)
@junghyunlee17147 жыл бұрын
Umm... I have one question. At the stacking gel, speed of the ions is Cl(-)>protein>glycine(n/-). In my opinion, Cl is faster than glycine these distance gonna be far each other. I wanna know details what is happened in stacking gel and these speed.
@soniyahalan90516 жыл бұрын
Thank you so much video is very helpful to understand better SDS page
@allysadati97104 жыл бұрын
True
@richardjoy85223 жыл бұрын
Really really really good explanation...I was searching for this type of detailed explanation in whole of KZfaq.....
@pavanhebbar58432 жыл бұрын
For SDS, I just watch this... Thank you for this indepth information mam🙏🙏
@daniellegoodwin35174 жыл бұрын
Thanks so much for a very clear explanation! I tried to understand the technique by reading a description online but it was so confusing, thanks for clearing it up for me!
@bharathreddy93376 жыл бұрын
I wish I will have a teacher like you in the future. terrific and detailed explanation
@maharmibhattacharya43275 жыл бұрын
thank you so much, the explanation was very descriptive and reasonably good.
@emilievu48465 жыл бұрын
Very well explained! Do you have any sources for this? I would like to refer to it in my masters.
@giorgiasartori63343 жыл бұрын
you are so amazing, keep going like this!! this is THE ONLY VIDEO i found explained well
@mariakln96545 жыл бұрын
Congrats! Very helpful video, I understood everything I needed for my lab. Thank you! (the explanation is very good and well constructed)
@gautamkannan19096 жыл бұрын
I have few questions. 1. Why do we have 8.3 pH in electrodes? What is the significance? 2. Is the gel already made or prepared during the experiment? Thanks in advance.
@jemalmohammed90116 жыл бұрын
the same to you
@joannchu145 ай бұрын
Beautiful explanation! Love this ❤
@MuhammadRizwan-dt5us6 жыл бұрын
i find it very useful thankyou so much for make that kind of videos
@harveenkaur17136 жыл бұрын
The most helpful video ever! Thank you so much!
@elmaspower23563 жыл бұрын
Awesomely explained.. Thanks very much mam. Lots of love and respect from Bangladesh
@user-eq8mc2hi3y3 жыл бұрын
Oh my god so useful video... you save my grade
@avishekdas1804 жыл бұрын
Nicely done yet again.
@ZAIUAMED7 жыл бұрын
Very helpful keep it up.
@darkgamingyt16593 жыл бұрын
Superb explaination
@sunnychatterjee54874 жыл бұрын
Explanation is fantastic but it will very well if you explain how stacking and separating gel is prepared...
@Ash-be1nv2 жыл бұрын
extremely helpful video for beginners
@allaboutfootball57296 жыл бұрын
Thank u very much u r wonderful keep making these videos I learnt a lot and many things become clear
@francestorreysirdevan47745 жыл бұрын
Superb video!! Insanely helpful! Thank you so much!!
@poetrylover55613 жыл бұрын
Very nice explanation of a difficult concept of SDS PAGE 👍👍👍👍
@shreyasen56145 жыл бұрын
Love the video! Very informative. Thank you so much
@roulala89934 жыл бұрын
Thank you very much for the video. You're a good teacher. 😊
@pannalalroat53943 жыл бұрын
Really such a amazing explanation 😘🤩
@punithagr33396 жыл бұрын
Clear and to the point. Thank you
@muhammadashraf-iy1gd11 ай бұрын
Outstanding explanation ❤
@biotechnologymahasarakhamu47577 жыл бұрын
Awesome teaching. Thank you so much.
@biomedicalandbiologicalsci49897 жыл бұрын
welcome ... Thank you for the comment :)
@preciousndzimandze43482 жыл бұрын
So helpful, thanks so much!
@batuhantekmen66073 жыл бұрын
Why and how proteins have been aligned in the stacking gel? Thank you.
@aydamohamed79833 жыл бұрын
Thnx sooooo much. Very clear and important
@AsNatCo2 жыл бұрын
Thanks Dr. what are the advantages and disadvantages of SDS page compparesmd to the native Page
@a_m13502 жыл бұрын
How can we distinguish the different proteins and their polymorphisms from each other after the separation. If, say our sample is cattle milk and we are interested in casein and all it's various polymorphism alpha, beta, kappa etc. Are we going to use the same or different markers to identify the required proteins.
@ruhiyyihdyrdahl-young38467 жыл бұрын
Thanks what a great explanation!
@biomedicalandbiologicalsci49897 жыл бұрын
You are welcome :D
@abidakhadim19356 жыл бұрын
Thank you mam .for giving a good visual memory of SDS.page .
@dancingbiologist26883 жыл бұрын
This was very helpful! Thank you!
@adityasinha11664 жыл бұрын
Thankyou so much for the explanation. I loved it 😊😊. Good work.
@simiralucialamberty59716 жыл бұрын
Your videos are excellent!
@kaushikilohiya21374 жыл бұрын
This is a really great explanation of d topic
@bobu5213 Жыл бұрын
I really don't understand why the stacking gel concentrates the proteins. If it is still a gel the proteins will be administered as they are pulled from the solution so they would enter the stacking gel separated. What is stopping the proteins from doing this? And how are the loose glycine molecules related. It doesn't seem like they have anything to do with the proteins, they are just there like chloride.
@ruchinambiar80053 жыл бұрын
This video was very useful. Thank you ma'am
@trish888813 жыл бұрын
This is so useful. Life Saver!
@elenatantardini49324 жыл бұрын
Best WB video ever!
@ashiqahamed77404 жыл бұрын
Very well put together. loved this video Thank you :)
@debjitdey2163 жыл бұрын
Finally I understood 🤗🤗🤗 thank uuuuuuu
@colin-kun36114 жыл бұрын
Solid video! Thanks a million times! Subbed!
@benguemichele49637 жыл бұрын
Thank you very much!!! I'm just from the lab where i did all this without understanding anything!! hahahahaha
@biomedicalandbiologicalsci49896 жыл бұрын
HAHAHA this is normal ... Sometimes we do things without going into the details ... stay tuned in the channel so you can see all the new videos :)
@priyadharshini-jx6jz6 жыл бұрын
So clear ,good job 👍
@tartanhandbag6 жыл бұрын
not clear on how the stacking gel helps. is the principle that in the stacking gel the protein migration speed is restrained by the glycine and clorine sandwiching it either side? so that when the glycine transitions to being more negatively charged and speeds up, it "gets out of the trunk"? need more explanation here.
@andrewfoster98235 жыл бұрын
I had the same question as you, and in case you haven't found your answer yet, I found some helpful information in my lab class's Theory Manual. After the chloride anions form the leading boundary in the stacking gel, the area immediately behind is rich in Tris cations and depleted of anionic species. This attracts anionic protein molecules and causes them to migrate at a rate similar to that of the chloride anions, at least initially, and protein molecules of identical size will be concentrated into a narrow band by the time they reach the separating gel.
@samuelgantner46095 жыл бұрын
@@andrewfoster9823 thx sir, that really helped. however i've two questions remaining: 1) what's the purpose of the glycin when the proteins simply get draged by the Cl- front? 2) how does the glycine behind the sample manage to get past it since it's supposed to stack it before? i know that it speeds up, but that stacking-process sounds as if the glycine fraction wasn`t able to get past it because otherwise there would be no sandwitching. to me, it seems to be more logical if the faster moving thing actually has to be behind the sample in order to stack it.
@TheDude-xf4pb5 жыл бұрын
@x4rrr had the same problem, now I was looking through my script again and came across the answer: Due to the fact, that the chloride-anions migrate at a very high speed through the stacking gel, behind this "layer" forms a zone with a high electrical tension (as @Andrew foster mentioned above, it's effected due to the positive cations of the buffer) The second quickest substance are the proteins, which are migrating through the stacking gel. Little proteins are migrating very fast, into the "positive zone" right behind the chloride anions but can't get much further at this speed, because the negative chloride is restraining them a little bit to do so (negatively charged). On the other side, very big proteins are migrating slowly. To avoid, that they don't get stuck too much, glycin pushes them through the stacking gel, so that's the positive effect of glycin. In Austria we call chloride because of that "leitionen" = leading ions, the glycine "folgeionen" = following ions. In the separation gel then glycin is overtaking the proteins, so they aren't pushed anymore and can separate slowly
@TheDude-xf4pb5 жыл бұрын
@@samuelgantner4609 maybe it helped a little
@pkjaihind34723 жыл бұрын
Excellent Explanation 👍👏👏👏 Keep it up 👍
@shdrone943 жыл бұрын
very good explained !
@orfeasorfeas93465 жыл бұрын
I finally understood it! Thanks!
@realmetv29702 жыл бұрын
Such a perfect video
@claudiaaurie81616 жыл бұрын
very good explanation. Thank u!
@alfankoshable16 жыл бұрын
thank you very informative ! keep going from Saudi arabia Salam
@priyashigoswami29985 жыл бұрын
Loved the video.. But why we don't use two gels in agarose gel electrophoresis like PAGE
@rajwardhanjagtap73295 жыл бұрын
Because agarose is mostly used for purification of DNA/RNA
@sundasshareef86916 жыл бұрын
Perfect .👍 simply wht i was actually searching for🙌
@Laladear-sz4hz6 жыл бұрын
thanku for clearing my doubts about this techniques
@M7MD99. Жыл бұрын
كثر الله خيرك
@StalwartsCo7 жыл бұрын
indeed best video for beginners.
@alterx88876 жыл бұрын
great teaching.. thanks a lot...
@biomedicalandbiologicalsci49896 жыл бұрын
Thank you for your comment .. welcome to my channel :)
@physicstutor91274 жыл бұрын
Very helpful. Thank you!
@vavakhannan33354 жыл бұрын
Very well explained........thank you so much mam
@deegallarupananda6927 Жыл бұрын
Thank you very much!
@ikz84392 жыл бұрын
you're helpful thanks!
@genomics37544 жыл бұрын
thank you mam....for such a well explained lecture.
@mychemicalfer5 ай бұрын
ty, this video helpme in the exam
@ArielStar5 жыл бұрын
Amazingly explained, thank you!!
@ariabaria1072 Жыл бұрын
Hi. at the end of the video, you mention that the proteins are not applied to the stacking gel at the same time, but they do so in the separating gel. What does this statement mean? what is meant by being applied to the gel at the same time?
@sylaslau3026 жыл бұрын
Thank you!! It's comprehensive and concise at the same time. I wonder what would be the technical difference (composition and steps) between this and Urea-PAGE for ssDNA or RNA.
@kashmirnature31343 жыл бұрын
Amazing' ❤️❤️ Thanks ma'am❤️
@krishnanandh79993 жыл бұрын
Very informative. Thank you😍
@firasbn15822 жыл бұрын
Thank you for the Video
@smart99245 жыл бұрын
Mam your videos are very good. It is very easy to understand this. Mam can you please make a video on immunoelectrophoresis. Thank you so much 😊