Quantitative Polymerase Chain Reaction (qPCR)

Рет қаралды 26,578

The Ahmed Lab: Northwestern Neurosurgery

Күн бұрын

Created by Shivani Baisiwala, BS, MS, MD Candidate 2021
Full protocols for all assays available at ahmed-lab.org
This video provides an overview of the theory and practice of quantitative PCR, a widely used technique in molecular biology laboratories in order to measure the mRNA expression of genes or to measure activation of gene transcription for specific genes. Here, we go through both the theory behind it and the practical aspects of getting primers, designing them appropriately, planning out your experiment, and interpreting and troubleshooting results.
This work is coming from the Ahmed Lab at Northwestern supported by funding from the NIH/NINDS.

Пікірлер: 26

@guptahittu 2 жыл бұрын

Thanks for sharing this wonderful presentation on qPCR. Detailed and very informative.

@anndiamond7601 2 жыл бұрын

Great presentation. Thank you very much for detailed explanation.

@sylvesteraddai-arhin5098 2 жыл бұрын

Great presentation, and very explanatory. Thank you!

@abolfazlnik-akhtar1667 2 жыл бұрын

In a word: Awesome. That was awesome.

@chrisjmolina 2 жыл бұрын

Thank you for your excellent videos!

@adnaniqbal4497 2 жыл бұрын

EXCELLENT . VERY WELL EXPLLAINED. CLEARED ALL MY CONFUSION. THANKYOU SO MUCH

@nizamuddinbulbul5942 2 жыл бұрын

Clearly explained....Thank you!

@mehran_mansouri 2 жыл бұрын

Thank you so much for sharing such a nice and comprehensive video

@gehadmahmoud6058 3 жыл бұрын

Very informative. Thank you

@jangidmanish6244 2 жыл бұрын

Thanks... Great tutorial

@neshatmd6924 2 жыл бұрын

great ! thanks!

@stevebow7337 2 жыл бұрын

Great presentation. Thank you so much for such a detailed explanation and also your personal suggestions from your real experiments. It really helps me in setting up my experiments and knowing what to do.

@dopefish3937 2 жыл бұрын

Great presentation, thank you! But I wanna ask qPCR introduced in your video means the Reverse Transcription-qPCR, right? However, quantitative Real-Time PCR is also called qPCR. Actually, quantitative Real-Time PCR and Reverse Transcription- qPCR are different, which is because the former doesn't involve RNA extraction and reverse transcription, but only DNA amplification. Generally, Reverse Transcription-qPCR maybe more often be named as RT-qPCR or RT-PCR.

@prof.dr.mohamedezzat8227 2 жыл бұрын

RT-qPCR or RT-PCR are not the same. RT-PCR is a conventional PCR but includes an RT step before the PCR, while RTqPCR is a real time (either quantitative or qualitative) PCR including an RT step either a one step reaction or a two step reaction, therefore when talking about real time, the standard abbreviation is q and not RT.

@YuetongUBC 2 ай бұрын

Thank you very much for your video! a small question: Is it possible to do the mRNA with a small sample size (e.g. 100,000 cells) for non-housekeeping genes?

@samihahzuramohdnani4137 2 жыл бұрын

Is it 1:10 working primer dilution right? Should it be ddH20 - 180 ul

@johnjakewinkler7484 2 жыл бұрын

Great video...but qPCR is not limited to quantifying the cellular expression of mRNA.

@hammedtukur 2 жыл бұрын

Excellent

@mohi7527 2 жыл бұрын

Excellent presentation, thank you! But all this explanation is about qRT-PCR and not qPCR. Am I right?

@romarichippolyteagassin3463 Жыл бұрын

We need to design a specific qPCR primers of the gene or we can also used the primers designed for common pcr?

@ahmed-lab Жыл бұрын

use this tool to design your qPCR primer www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool?src=google&gclid=Cj0KCQiA8aOeBhCWARIsANRFrQGM38dfc7LgVjoEkIVsjGq5-PZACD6BPEfMpYtlv1sBrioS_xZpK9saApwjEALw_wcB

@romarichippolyteagassin3463 Жыл бұрын

@@ahmed-labThank you very much. I appreciate 🙏

@osmirod1 2 жыл бұрын

You’re a god !

@hystrionic 5 ай бұрын

i'm interested in the info but the vocal fry is making it hard to listen.

@samuel_prince Жыл бұрын

14x 🤔🤔

@walkerchen7336 2 жыл бұрын

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