Practical tips for pouring plates: cool LB/Agar in a 55C water bath & remember the stir bar!

  Рет қаралды 1,306

the bumbling biochemist

the bumbling biochemist

Жыл бұрын

Practical pointer for preparing plates: While you’re autoclaving your LB/Agar solutions, set a water bath to heat to 55°C. Then, after autoclaving, transfer your too-hot-to-do-anything-with-yet bottles to the water bath.
Now you can safely forget about them! Well, don’t actually forget about them. But if you put them in the bath you won’t need to worry about monitoring them closely to prevent them from over-cooling and starting to solidify.
At 55°C, the solution will stay liquid, but it won’t be too hot to add antibiotics and pour. And, since it doesn’t start solidifying until ~45°C, you will have enough time to do all that before it gets gelly.
It’s important that you don’t add antibiotics while the solution is really hot because this can “break” (degrade) the antibiotics, negating the point of adding them… And it’s also important not to pour the plates when it’s too hot because you could damage the plates - or your hands! But 55°C is ideal and comfortable enough to pour.
So, go ahead and do some other stuff for a while while the bottle gets to that temperature, and then go ahead and add your antibiotics, stir, pour, and enjoy the aroma!
Another tip: autoclave the bottles with a magnetic stir bar in them (don’t worry, they’ll be okay in the autoclave!). This way, you can stir on a stir plate in order to mix the antibiotics, avoiding the bubbles that come with shaking.
more on LB/agar plates: bit.ly/agarbakery ; KZfaq: • Agar vs agarose - what... & • LB agar plates - theor...
more random practical lab tips and tricks: bit.ly/lab_tricks_page   
           
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 bit.ly/2OllAB0 or search blog: thebumblingbiochemist.com

Пікірлер: 3
@rakshith1994
@rakshith1994 4 ай бұрын
great suggestion!
@gulliverbear
@gulliverbear Жыл бұрын
how long do you leave plates to dry before putting them at 4 degrees?
@thebumblingbiochemist
@thebumblingbiochemist Жыл бұрын
depends on my schedule... sometimes overnight
IPTG induction using the lac promoter
8:20
the bumbling biochemist
Рет қаралды 4,2 М.
LB agar plates - theory & practice
27:01
the bumbling biochemist
Рет қаралды 1,1 М.
HAPPY BIRTHDAY @mozabrick 🎉 #cat #funny
00:36
SOFIADELMONSTRO
Рет қаралды 17 МЛН
DAD LEFT HIS OLD SOCKS ON THE COUCH…😱😂
00:24
JULI_PROETO
Рет қаралды 13 МЛН
Insane Things I've Found In Dead Bodies
11:57
Institute of Human Anatomy
Рет қаралды 81 М.
41 Tips to Make Your Entire Home Cleaner Than It's Ever Been
21:43
Jansen's DIY
Рет қаралды 10 МЛН
Amish canning Part 1-Water bath everything.
17:57
Makeitmake
Рет қаралды 578 М.
Making LB Agar Plates
14:57
Professor Drew Collop
Рет қаралды 8 М.
How To Form, Pour, And Stamp A Concrete Patio Slab
27:12
Mike Day Concrete
Рет қаралды 4,6 МЛН
How to get crystal clear agar plates for mushroom cultivation
6:51
Fresh from the Farm Fungi
Рет қаралды 69 М.
How to make: Homemade agar plates
11:35
Dr.Chobbsy
Рет қаралды 93 М.
Tips for Water-Bath Canning For Dummies
3:15
fordummies
Рет қаралды 621 М.
Tips for Bradford assays (or other microplate assays) without bubbles
2:09
the bumbling biochemist
Рет қаралды 642
ОБСЛУЖИЛИ САМЫЙ ГРЯЗНЫЙ ПК
1:00
VA-PC
Рет қаралды 2,1 МЛН
СТРАШНЫЙ ВИРУС НА МАКБУК
0:39
Кринжовый чел
Рет қаралды 1,4 МЛН
Сколько реально стоит ПК Величайшего?
0:37
Копия iPhone с WildBerries
1:00
Wylsacom
Рет қаралды 485 М.