“They take the glass butt. Sorry: they take the glass, but…” Gotta respect the ZeFrank tribute

the snails might be missing minerals for shells and this might be the reason they keep on dying .

I wonder if too many lifeforms that depend on calcium were born, that might explain the die off. Anything with an Exoskeleton or shell will draw calcium from the water, and over 4 years with such a small environment, its possible all the available calcium is tied up in the exoskeleton and shells of the various lifeforms. I wonder if a Jar with some crushed coral to buffer the water would allow for the long-term survival of a snail population.

Eight Eyed Blood Hedgehog Cool band name unlocked!

That football field comparison was very helpful thank you

Instantly clicked.

I appreciate how seriously he takes his presentation's. Seeing him introduce the topic while sitting confidently in a suit really cement's the science vibe and I love it.

RIP our visually gifted and veiny, spiky mammal imposter. ❤ We will miss you.

As someone who has kept aquariums, I can tell you that the algae growth in lines is usually because there is some structure on the glass that it clung to that just so happened to be in straight lines. Sometimes its scratches, sometimes its just a bit of dirt that got wiped on the glass, but algae like in straight lines is almost always caused by this

I know my isopods sleep. They like to hide under wood and bark pieces and have a nap. I know this by how long it takes some of them to react when I turn over the piece of bark. When they wake up, they run away and try to hide again.

RIP boogieworms, you will all be missed

Tracing back my youtube history over the last year or so, I think you are the reason I now have a fulfilling aquarium hobby! One of your videos randomly came up and slowly got me more interested in microorganisms and the elegance of the food web in every ecosystem.

9:18 as an American, this really helps, thank you. Edit: As a recently new viewer of your channel, I am excited to see new videos and updates of series like this one.

1/18,000 of a football field is what stood between me and a career in the NFL. I'm an alcoholic now, I will never forget that measurement.

I was expecting to see the critters evolve sunglasses. 😎

@8:00 You know, it could be that the snails you're viewing are actually adults that have undergone a sort of island dwarfism process. How many generations is four years worth?

You are quite a charming, and intelligent content creator. Instantly a fan.

3:10 wags his shell like a dog!

Have you considered combining a bunch of your old jars together in a larger aquarium? All the things left at the end of experiments are hardy in one way or another. Maybe make an aquarium with an always dark hidey hole, a corner that always has the lights on, etc...

Maybe you got some sort of toxic buildup in the substrate, gas for example. When it finally came out it produced a mass extinction killing adults. Once other microbes consumed the toxic compound the critters hatching from the eggs repopulated.

I'm very happy to see you've been in your fancy pants a lot lately. I hope this means you're feeling a lot better lately! It's always very nice to see you! I really liked learning about your no light ecosystem!!

I can't believe it's been 4 years. I remember when you started this one

Sir David Attenborough shoild be watching his back… there’s a new nature narrator in town!

I kept (and still have) a airtight jar 6 years on and at about 3-4 years, the snails all died out. I never did ascertain why. I just assumed the balance of minerals or oxygen in the jar tipped and a mass extinction occurred. Only mine had a day/night cycle.

you're probably in my top 5 channels man. I'm so glad you're back in full swing, huge inspiration to my

The sunlight being darker than the LEDs must be their night time lol

I love the ducks in the background, very entertaining

had absolutely no idea what you meant by "half a centimeter" until you converted it into a proper, easy to understand unit... thank you!

What an exciting update!!!!! Thank you for the video!

How many closed ecosystem do you have? I would love to see a video of you showing your full collection of jars

Any follow up this far in deserves a like

Oh woah I remember the making of this thing. Never thought I’d see it four years later! Cool stuff

I can not express how much i enjoy your personal snip its

I still can't believe how different Jars looks from how I imagined him from his voice

Very interesting follow-up, thanks!

I saw Eight-Eyed Blood Hedgehog in concert back in ‘93. Epic show.

Thank you for sharing your knowledge.

Love these videos

Always a joy to see!

This is the first time I was suggested a video of this subject. I'm intrigued!

Love your sense of humor!

I do wish it was possible to pay closer attention to figure out the how and why of the extinctions.

Conteúdo primoroso!! Parabéns!!!

I’m so glad he converted the length for me, it really help.

I would love to see you go ahead and open a bunch of your longer-lived eco-jars and take representative samples from them in order to make a succession jar of species that seem to cope well with tight nutrient loops. If you have any terrarium, you could use some of the soil from it and some long-lived plants and make a succession paludarium.

I have no idea why this was recommended to me but it was very entertaining

Great video! I thought the 24 hr. light would have more of a negative impact on the animals. I suppose it makes sense that putting more energy into an ecosystem would cause it to thrive. Maybe an interesting idea for a future project would be identical ecospheres that receive only certain wavelengths of light. Maybe one that gets only UV or infrared light?

The best channel to grace this website is before us, praise to the dapper don.

I read that monks at a temple or shrine in Japan, they raise bell crickets that sing during the day. Normally, they only sing in the dark, but according to the monks, the technique was to raise them in constant light.

10:49 that duck just fit's in perfectly 👌👌

American here. The football field measurement truly made me laugh

Fascinating stuff! Thank you. It's this kind of channel that the internet was made for!

I am really curious what the difference between two jars would be if you filled the top part of one jar with pure oxygen and the other jar with pure CO2.

Let me guess: free floating algae. I've done this as well with the same result. The reason is algae have short life spans and are capable of rejuvenating themselves efficiently during cell division. Most other plants require dark periods occasionally for 'housekeeping'.

The lean in for that joke absolutely sold it.

Love the weird 50s era television host energy you're channeling

I just loved the laugh at the end.

Hell yeah, new LiJ video, dressed for the occasion aswell, bravo. Props to the dutch for naming it ''eight-eyed blood hedgehog"

I remember when you first did this project and being fascinated that you had created an environment that in theory couldn't exist.

YOOO BABE WAKE UP NEW LIFE IN JARS

9:13 blud rlly screamed

I recently got LED strips, so now I can make some jars of life in my small apartment!! Your videos drive curiosity, very inspiring. Thank you for making these videos :)

thank you

Instant click! I wish you well, Life in Jars!!

@4:33 Who is this comedian who also does experiments with closed ecosystems? Truly a man of class and refinement... :P

This is exactly the type of content I've been looking for, for years now. Many thanks! Do you know of any studies like this with different ecosystems and could point me to them?

❤ I've had 2 closed ecosystems, the seed shrimp were my main survivors, does get day night but never really opens , somehow I still got frogsbit , 2 years running

I fn love this channel

3:12 it looks like it's waving hi!

Why did KZfaq need to show this to me? Why did i clikc it? Why did i watch it all? Love it

For real; Damn neat channel! Would sensors for telemetry be possible?

Maybe your copepods are smaller due to living in a smaller environment (in this case a jar) for the last 4 years. A bit like island dwarfism or something?🧐 I’m no expert in such things, but maybe something as small as those could adapt in that timeframe. Very cool video my friend 😎

Good video

Altho ostracods and copepods may or may not sleep(I’m sure they most likely do) almost all organisms we have studied have a circadian rhythm, or an internal rhythm which roughly takes about a day to complete and syncs up with the 24 hr day on earth primarily through the light and dark cycle provided by the sun rising and setting. When organisms are removed from an external cue that syncs up their internal clock to the external world, their internal clock starts to “free run”. Basically pretty much no organism has an internal clock with a periodicity of 24 hrs, so it will start to slowly(or rapidly if greatly different from 24 hrs) get out of sync with our actual clocks until a nocturnal animal starts to come out during the day for example. In this experiment that you set up, you are essentially removing that external cue by subjecting them to constant light, Altho if you’re turning off the leds during the day that subtle change in amount of light received might be enough for their internal clocks to sync to, but these creatures will still go through periods of high activity and low activity, possibly even sleep, but it just might not be in sync with the external day night cycle. Another interesting thing tho is that these organisms might act upon each others internal rhythms bc circadian rhythms aren’t only synced up to light, but a myriad of other factors, and I’ve always thought it’d be interesting to see how mixing and matching different species would affect their activity patterns and free runs. Sincerely, someone working in a lab that studies chronobiology😉

1/18 thousandths of a football field legit made me spit my drink lmao

Just discovered your content and really like it. Keep this kind of stuff coming! *Don't keep us in the dark* 🙄

Thank you for sharing that fascinating experiment. I thought the flatworms actually looked quite cute.

The need for light dark cycles is fascinating. Years ago we were doing a canola (oil seed rape) study in a growth chamber and things were weird. The plants were stunted and turning purple. We finally figured out the chamber had been turned to 24 hour light cycle instead of 12/12. Did we feel foolish. The purple plants which more closely resembled their cabbage cousins were cool though.

1 eightteenthousanth of a football field really put it into perspective for me. Thank you for thinking about us! 😊

I have found the biggest factor with snails failing or succeeding is temperature. If it can be kept at a constant temp they thrive.

Now I'm interested in how much it would change if it started getting a normal day and night cycle

Looking sharp

Instantly liked.

I once found a jar in the back of a garden half submerged in the ground, I noticed it was full of the same kind of plants that surrounded the jar, but all the plants inside were tiny. I wonder if it's possible to grow ganja in jars? You should do a bunch of weird jars for science, like jars with crystals or metals or magnets or trash, Or try some Masaru Emoto style experiments.

It would be interesting to do this again but split the water between 3 jars. One with 24/7 light, one with 24/7 dark, and one with a 12 on 12 off light cycle.

Yippie the silly jar guy returns once again! (To make a video)

Man im glad KZfaq recommended this too me i figured they forgot i cared

Interesting science project! The snails might be missing minerals like calcium or magnesium (the GH might be too low) or the water might have become more acidic (low pH), both will lead to shell-problems for the snails and affect adult/larger snails the most. It will also effect pods. If repeated, you might consider adding a GH/pH buffer and see if this will effect long term results. There are also slow-release fertilizers for aquarium substrate you might try out. O2 levels are also surely dropping creating anaerob conditions, as oxygen is incorporated into organic material. You might consider "carbonating" the water with CO2 initially before you close the jar, will increase growth of algae/plants and provide extra source of oxygen molecules.

Thank you oscar piastri. very cool

9:33 "They take the glass butt!" I see you must also be a fan of Ze Frank!!

i would have never understood how long the flat worms were if not for the 1/18000 of a football field thank you so much

I JUST WATCHED THE LAST UPDATE ON THIS ONE LAST NIGHT WHAT ARE THE CHANCES YOU POST THIS TODAY!

9:14 thank you this really helped 😂😂

I think a great follow-up would be to repeat the experiment but use a filtration system to offer a cleaner environment. Use a second jar with filtration and normal sunlight as a control... two for a better comparison.

To collect specimens why not use a wide sterilized pipette? You can leave it in the water near a flat worm then when they get close use some fast gentle suction to suck them up into it. Then you can place them in a petri dish for better viewing and the whole procedure is minimally invasive.

The preview at the start looked like the surface of venus lol

just an idea for a tiny ecosystem: Can you make an ecosystem with higher than average O2 by using a small electrolysis set up and a bubbler? I heard that arthropods grow larger in high oxygen environments, and it seems doable on the small scale. Just vent out the hydrogen to make sure it doesn't accumulate in a confined space.

Very interesting! It should be very interesting if you take some living sample and send it for genetic analysis (some with short generation). The changed environment in your experiments like this will cause interesting genetic changes, (directed evolution). Or you can experiment with an closed ecosystem irradiated with a single wavelength (monochromatic LED) and observe changes in the algal photosystem.

question, why did all the multicellular algae die? what does the unicellular algae have that allows them to thrive better in this environment

I’d like to see this same experiment with different colour lights. Have 4 jars with the same water and mud with the only difference being the light colour.