Goodluck on your test!

Aliia T thank you!

Hope you did good on your test lmao

tf

Aliia T same!!!!

(in asmr voice) Happy to know that my videos have started to have more than one application; helping some in their bio while also taking the life out of others ;) (No pun-casm intended)

Hi, you can try leaves of different colours and shades. I've also seen some videos where they separate the pigments contained in ink. You can also separate amino acid components in a protein rich food source. Although they will need a visualizing agent to be sprayed on to them and then slightly heated to be able to see the amino acid spots on the paper. But for this u will need Ninhydrin (a potent carcinogen) so i highly doubt if thats the right thing for a sc fair projct. So u can work on Leaves and inks. Good luck 👍 i'm here if u need help.

Thank you so much man!!

Hope u did well in ur exam. I am from Manipur

Each spot is a single phytopigment. From the first spot (chl b) the remaining pigments except chl b will migrate to the second spot (chl a spot). From second spot, all other pigments except chl a will migrate further up to the third spot and so on. This groups migration and the leaving behind of one pigment type at each "stop/station" will continue until all the pigments have been resolved into different spots on the paper.

You can perhaps use a mix of leaves of different colors; green, red, yellow, orange...... Or you can use a mix of different coloured inks. Never actually tried these "fun" experiments before though. Good luck 🙂 do lemme know the results. 👍🏾

you may substitute it petroleum ether with something that is non-polar such as hexane.

If u dont get the chlorophyll and carotenoid bands/spots. U need to recheck your solvent and use quality whatman paper.

standard textbook knowledge. This is a highschool level experiment performed as part of syllabi, based on experimental facts already investigated decades ago by pioneers in chromatography.

@@TheSingtangpaScienceGuy thank you very much

i used amaranth leaf, as it was just nearby me. You can use any leaf as long as its green.

1:9

Had to pull it down for various reasons. Will come up with a better vdo in the foreseeable future 😁

What plant species, what solvent (or solvent combination), did u use?

@@TheSingtangpaScienceGuy we used Sauropus sp as the plant species and an ether acetone solvent

@@vishesharrvansashitharan3593 its probably because the leaves from which you prepared the extract, are still young, green and actively photosynthesizing. Pheophytin, as you might have already known, is one of the breakdown intermediate products of chlorophyll, and this breakdown into pheophytin, takes place usually during leaf senescence. So, even if the fresh green leaf might contain a little bit of pheophytin, the quantity is not so much as to to be seen on a chromatogram. Ofcourse, if you do a spectrophotometric analysis , you will detect some amount. But If, for any reason, you still want to see pheophytin on the paper, you can acidify the leaf extract prior to loading on the paper, in that way the chlorophylls lose their Magnesium and become pheophytin. Or you can make a leaf extract from old, senescing leaves that have yellowed. You are sure to find a nice pheophytin band/spot from old leaves. This is all i know from my textbook knowledge. Correct me if i'm wrong. Goodluck.

@@TheSingtangpaScienceGuy alright that was very helpful, thank youuu soo much , god bless you , this is the actual experiment for my practical test next week, again you've helped sooo much , thanks🙏

Hi. Volume problem has been adjusted from subsequent videos. Enjoy !