To investigate the effect of temperature on enzyme activity:
The enzyme used in the three experiments focused on the rate of activity is Catalase which is obtained from celery, its substrate is Hydrogen Peroxide its product is oxygen (seen as foam in the graduated cylinder as the oxygen is trapped by the washing up liquid) and water.
All three experiments are set up using the same volume of catalase solution (obtained by blending some celery), pH buffer and a few drops of washing up liquid in a graduated cylinder. The same volume of hydrogen peroxide is then added to the graduated cylinder and the rate of activity is measured by tracking the volume of foam produced per min after adding the hydrogen peroxide
• The temperature is different for each graduated cylinder as water baths of different temperature are used each time.
• The pH is kept the same (pH=9) by using a pH buffer.
Result: The graduated cylinder placed in a water bath of 25°C works best