Transcription Activator-Like Effector Nucleases (TALENs)

Рет қаралды 64,761

9 жыл бұрын

In this video we discuss a type of synthetic nuclease, namely the TALEN, standing for transcription activator-like effector nucleases.

Пікірлер: 71

@payalwankhede1393 2 ай бұрын

Finally I got it. This is the best explanation available on the internet. Thanks

@navadeepdorai2918 2 жыл бұрын

Old standard of teaching... A gold standard 👍🏻

@grace96342 Жыл бұрын

our college needs you as our professor, you explained something in 10 minutes which our lecturer failed to present in a 1h lecture. THANK YOU SO MUCH this helped me a lot

@md.golamazom2703 4 ай бұрын

Great explanation on TALEN I found on KZfaq so far

@MrPelafio 6 жыл бұрын

I was just about to google difference between TALEN and ZFN, when at the very end you explained it. Wonderful!

@harryli2053 7 жыл бұрын

Best explanation ever! Thanks so much for your clear explanations and visual demonstrations!

@monthappy2521 3 жыл бұрын

Dude, I absolutely love you 💕 Greetings from Colombia.

@talimachet2419 3 жыл бұрын

You have saved my life - I am eternally grateful. Thank you so much!!!!

@meriamtayar8982 5 жыл бұрын

You are absolutely amazing. Great explanation! Thank you!

@mayso1104 7 жыл бұрын

this is the best explanation I've seen. Thanks!

@tereabentley5896 9 жыл бұрын

Thanks, this is a great explanation. Xanthomonas FOK1

@susanavazquez224 4 жыл бұрын

It is excelent explanation and so great it is done the old way of personal teaching, paper pencil and a great teacher! congrats!!

@condimentado 7 жыл бұрын

BEST EXPLANATION EVER!!!!

@albertobray6963 7 жыл бұрын

AWSOME explanation!!! Thank you so much

@paygeshelton6252 5 жыл бұрын

Thank you so much!!! This was amazing and easy to follow!!!

@ozanatasoy5591 8 жыл бұрын

Thank you friend. As a virologist it's very simple and informative explanation for me.

@g-host6527 Жыл бұрын

32 min left to study before test, this dude saves my life

@askarrafi243 4 жыл бұрын

Thanks for your complete and clear explanation. It was awesome.

@changachangueria 7 жыл бұрын

WOW!! nice explanation!! TY so much.. great job

@chutimon.ceefang 7 жыл бұрын

Thank you so much this helps me a lot.

@mahachris5477 8 жыл бұрын

Thanks , you made it look so simple.

@lukapreloznik7123 4 жыл бұрын

I'd give you a HEART, but only LIKEs are available on yt...very good explanation, thank you very much!!!

@aquamarine3699 3 жыл бұрын

Thank you for the clear explanation!

@harleyjerram9699 8 жыл бұрын

Thank you, really nice video!

@jeminmangukiya8655 3 жыл бұрын

bloody brilliant

@misabella323 3 жыл бұрын

Thank you sooo much!!

@pauladalcinmartins7421 8 жыл бұрын

Great explanation!

@JMmitchell633 7 жыл бұрын

Great explanation....thanks!

@sancharan6342 3 жыл бұрын

Again.. sir you are awesome.

@houmamkitet9555 4 жыл бұрын

Very nicely explained, thank you very much for making the video

@karamella565 3 жыл бұрын

thank you very much.

@angeloorsini1764 6 жыл бұрын

The best one on youtube. Thanks man!

@sarahbrm253 6 жыл бұрын

the best* one ..

@angeloorsini1764 6 жыл бұрын

thx

@gregorysmith1134 Жыл бұрын

Excellent explanation.

@joannamaw8920 2 жыл бұрын

Thanku for this!

@sapastje 7 жыл бұрын

helped a lot, thx!

@annakassis8175 6 жыл бұрын

That's the best explanation ever, (Fok1)

@MonsieurDepadieu 4 жыл бұрын

Thanks, great explanation clear as Clearacil®

@Nico14071997 5 жыл бұрын

Take a shot every time he says "transcription activator like effectors"

@christenemelad5435 5 жыл бұрын

Excellent work

@hoimebanerjee1066 4 жыл бұрын

Nicely explained. Thanks 😊😊

@XamaL1 7 жыл бұрын

Thank you !

@Neo-rd7ef 7 жыл бұрын

Thank you very much

@mgold9999 5 жыл бұрын

You could have mentioned the 34 aa repeats in the TALE where aa 12 and 13 are variable and specify the DNA base maybe.

@ayaqz3144 3 жыл бұрын

thank you

@deblinachakraborty5231 Жыл бұрын

😊😊make more vedios sir, we are in need.

@Christina-eq8yc 7 жыл бұрын

THANKS!

@gasperkosmac7672 8 жыл бұрын

THANK YOU

@Hansel551 Жыл бұрын

Nice.

@celinec6366 Жыл бұрын

Is it true that Fok l cuts 9 nucleotides downstream in the first strand and 13 nucleotides upstream in the other strand? I didn't understand it from your movie.

@serventofalfaandtheomega3040 3 жыл бұрын

Thanks

@lend2150 Жыл бұрын

Thanks for this video. You simplified it well. May I correct that it is FokI or Fok1 (Fok - One not Fok L) and it is Xanthomonas (not Xanfhromonas, it is t not f).

@paucska 4 жыл бұрын

very good explanation, thanks! Although: what are the chances these sequences getting attached to the wrong place? :O I mean there are some chances, aren't there, considering the very lengthy and extensive size of the human genome...

@Samsu7777777 Жыл бұрын

Ultrasonic lecture

@056vatsalapandey3 2 жыл бұрын

thank you booooooooooooooo

@sudiptasarma 3 жыл бұрын

So, on the paper, it says that RVDs of TAL effectors correspond directly to the nucleotides in their target sites, one RVD to one nucleotide..my question is TALeffector are amino acids only, so how can one amino acid recognize one nucleotide? it is generally, three nucleotides represent one amino acid..or correct me if I am wrong please, bit confusing

@aurberdyson1940 2 жыл бұрын

Uh... I'm curious, but not professional. I've seem many books write Fok"I"(I i) instead of Fok"l" (L l). So, could anybody tell me which is right? I'll be appreciated. :)

@ChengVincentckf 9 жыл бұрын

Great explanation, I am not sure that is FokL or Fok I

@ardalanmansouri8176 8 жыл бұрын

It is actually Fok1 (or capital i as a Greek number instead of "1"). For more information regarding its discovery please go to the following link published by the first researcher discovered it: www.ncbi.nlm.nih.gov/pubmed/6282705

@benjiprice8822 8 жыл бұрын

are you talking about restriction enzymes or is it something completely different? please do answer me, how about you @ozan ATASOY

@mariahfoley3645 7 жыл бұрын

What about RVDs

@dia6976 9 ай бұрын

talens by XANTHOMONAS BACTERIA

@ally850 5 жыл бұрын

skip to 2:56, first few mins are bogus

@cannizzosam5417 5 жыл бұрын

* FOK1 not FOKL

@aronimitra28 6 жыл бұрын

RVDs in a particular TALE recognizes different bases. So change in RVDs results in change in sequence specificity. 9 TALEs don't copolymerize. Only two TALEs with different RVDs bind to two adjacent sides of Fok1 recognition sequence. You're explanation is pretty much wrong.

@nidhyajoseph1457 6 жыл бұрын

Can you please elaborate? I did not get what exactly was stated wrong here.. Also how are both the TALEs and REN -eg. Fok 1 made so specific for a particular sequence? Both the TALES are different sequences right? Are there any chances of both these TAL effectors binding to each other..?

@aronimitra28 6 жыл бұрын

Nidhya Joseph TALES from Xanthomonas has a DNA binding domain and an activator domain which allows it to regulate the activity of certain genes in it's host. The DNA binding domain has 30 repeats of 33-35 amino acid residues. Out of these 35 residues, residue number 12 and 13 are variable in the repeats and thus they are called repeat variable diresidue. These two amino acids are responsible for recognition of a single nucleotide in DNA. Amino acids NI at RVDs recognizes adenine, NG recognizes thymine, HD recognizes cytosine and NN recognizes adenine or guanine. So if you know the sequence of your DNA then you can design TALENS that bind to your specific sequence by changing the amino acids at 12 and 13th position of each repeat. In this way the talen can bind to your sequence and the DNA cleavage domain of Fok1 fused to the DNA binding domain of TALE can cleave the DNA. Generally two TALENS have to be coexpressed in a cell, one that binds upstream of the cleavage site and one that binds downstream. When the two will bind, two Fok1 domains come in vicinity and cleave the DNA molecule. The cleaved DNA can now be joined by non-homologous end joining which is an error prone double stranded break repair process , leading to incorporation of mutations in your desired sequence of interest.