Ultramicrotomy: The Art of Sectioning for TEM
Рет қаралды 29,762
11 жыл бұрын1. Trimming
2. Small Section
3. Large Sections
While learning how to section I found it difficult to understand the concepts without a visual aid. For my final project in the TEM course I created this video to aid other visual learners. I apologize for the poor quality but hope that this video is informative.
This video is for educational purposes only.
@TheJdhisto 14 күн бұрын
Things are much simpler these days. There are many unnecessary steps and techniques included in this video that are not currently needed, and the ultramicrotome has advanced drastically.
@jivkokatz 3 ай бұрын
Great video! Thanks for uploading. Music made it even more epic ;)
@joelturner1422 5 жыл бұрын
Really good explanation of the steps involved! Thank you!
@chiaruscuro 9 жыл бұрын
Excellent video. Thank you for this!
@guangmeihuang1993 9 жыл бұрын
Yes very good! Thank you!
@reemmatrook1104 9 жыл бұрын
Very useful,,,Thank you very much!
@molkhal 3 жыл бұрын
This was so satisfying to watch
@blanca4287 9 жыл бұрын
This will be great for my students! It's so hard to crowd around a microtome, but this video will help with that! :)
@matthewc7138 7 жыл бұрын
Where do you teach?
@Lifewithcolors59 4 жыл бұрын
This was so helpful thank you very much❤️
@alphonsagrace1013 4 жыл бұрын
Thank you.
@hassansadek3665 5 жыл бұрын
thanks a lot, this video is very useful
@lisseliza 6 жыл бұрын
i am appreciating the tron soundtrack
@pooltrader 9 жыл бұрын
wow, so cool, ty
@dongliangli7658 4 жыл бұрын
Very useful，thx!
@humanhealthcom 3 жыл бұрын
Grate sir
@junglebaby448 5 жыл бұрын
what camera did you use to record this??
@danielvocelle1414 5 жыл бұрын
Just the camera on my phone
@junglebaby448 5 жыл бұрын
@@danielvocelle1414 did you just mount the phone?
@danielvocelle1414 5 жыл бұрын
@@junglebaby448 yes, built a holder out of wood to hold it on the objective
@basisTermium 4 жыл бұрын
How small is 70nm just curious
@hectorherrera526 3 жыл бұрын
Smaller than the diameter of the coronavirus. Coronavirus is approx. 100-120nm
@lotharmayring6063 Ай бұрын
i dont think that it is nessesary to make marks for trimming becuase anyway you are not cutting on the mark
@MarySmith-gu5nb 3 жыл бұрын
I hate the soundtrack. Makes it sound like something horrible is going to happen.
@danielvocelle1414 3 жыл бұрын
Thanks Mary
@robinhowes6694 5 ай бұрын
Block too far out of the holder. Dreadful score marks on the sections - throw that knife away ! Fit a new one or use a diamond knife
@danielvocelle1414 5 ай бұрын
Happy to report knife was thrown away 10 years ago.
@robinhowes6694 5 ай бұрын
I'm glad you did! I started doing ultramicrotome in 1966 using a completely manual Cambridge ultramicrotome, with glass knives, cutting phloem tissue. Very difficult! I moved to the Institute of Ophthalmology in 1971 and finally ended up using a Leica ! I retired in 2011,after seeing many changes in TEM and SEM Happy cutting !