Título: Sample Preparation for the Analysis of Volatile compounds by GC-MS
Descripción: Preparation of red ripe tomato pericarp samples for the extraction of volatile compounds by headspace techniques. López Gresa, MP.; Caballero Vizcaino, M. (2019). Sample Preparation for the Analysis of Volatile compounds by GC-MS. hdl.handle.net/...
Descripción automática: In this video, the presenter outlines the method for preparing samples for the analysis of volatile compounds using gas chromatography coupled to mass spectrometry (GC-MS), particularly within the field of metabolomics research. The goal is to effectively isolate volatile metabolites from a fruit sample.
The necessary equipment and reagents are listed, including liquid nitrogen, various chemicals and buffers, specialized vials and caps, and equipment like a water bath, sonicator, and a gas chromatograph-mass spectrometer setup. Specific mention is made of the need for an automatic sampler and a precise type of GC capillary column.
The sample preparation process is described as simple but requiring meticulous execution. Steps include thawing the pre-weighed sample, adding reagents to halt enzymatic reactions and stabilize the volatile profile, and ensuring the sample's homogeneity before sealing it for analysis. Emphasis is placed on the importance of consistency in the procedure, as variations can affect the results. The stability of the volatile profile within a time frame for reliable analysis is also highlighted, along with the importance of a well-designed protocol tailored to the sample type to ensure accurate GC-MS analysis.
Autor/a: López Gresa María Pilar
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#HSPME #VOCs #2302 - Bioquímica