Presented By: John Yan, PhD
Speaker Biography: Dr. John Yan is an Applications Chemist for the Bioconsumables portfolio within the Chemistries and Supplies Division of Agilent CrossLab group. John joined Agilent as part of the ProZyme acquisition where he was an Applications Scientist supporting glycobiology reagents and N-glycan sample preparation and analytical workflows.
Webinar: Workflows for Glycosylation and Sialic Acid Analysis of Biotherapeutic Glycoproteins
Webinar Abstract: N-Glycans on biotherapeutics can affect immunogenicity, pharmacokinetics and pharmacodynamics, making characterization of N-glycans essential in the development process. Here we present rapid N-glycan sample preparation and analysis workflows for biotherapeutics, including released N-glycan labeling with InstantPC and 2-AB for characterization by liquid chromatography (LC) with fluorescence (FLD) and mass spec (MS) detection. In addition, we will discuss approaches to identify different sialylated N-glycan species as well as quantitating the sialic acid content of biotherapeutic glycoproteins using plate-based and LC/FLD/MS approaches.