This is very helpful, but how can we add that new fluorophore to the online spectrum viewer?

Hi can you do a video explaining the gating using pbmcs

i know your son Mr. Sederstrom🙏

I see you extracted autofluorescence from the lymphocytes, then looked at the impact on the monocyte population, is there a way to create two autofluorescence gates for each population and do the autofluorescence extraction from both populations, but have it applied to only certain gates? Or do you have to essentially setup two experiments, one for each autofluorescence

Hi This is Bapi Pahar from NIIAID, IRF. Just want to let you know the long clean tube assembly shown by the representative does not match with the figure shown (time 3:36 slot). Please correct it. Thank you.

i forgot how to do this and here i am jamming to some funky music 🎶

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Thank you for the video. I have a question: why did you say that the side scatter is off at the UV laser?

is the reference group the same as the compensation control?

I like XXL size

Any competition for this product?

Great video's. Very helpfull.

画質が悪くて、文字が小さいので見えにくいです。改善できませんか？

What's Rui doing over there??

The figure is small and hard to see, but can you enlarge it or enlarge the characters in the software?

Thank you for the series of helpful videos! Is there any way to adjust the gating on the references controls after they have been stored in the library? I have found my samples to be very heterogeneous (also stain multiple different tissues which require unique unmixing strategies) and I usually have to adjust the gates on the positive and negative peaks of some markers multiple times before I end up with an acceptable unmixing.

The music is so annoying ! Doesn't letting me concentrate !

Thanks for the video. I have some questions about ASF, hope you will answer. 1. What is the mathematical relation between A,H and ASF? 2. What it physically mean when ASF is greater or smaller than 1? If the laser is not positioned at the center will it change ASF? 3. Cartoon at 7.32 minute, what happens if we just extrapolate the pulse shape upwards so that the two end can meet and calulate the area from there? Will it work if not why? 4. What is the effect of ASF on unmixing? 5. If I have a 20 color panel will you still recommend to check/change the ASF for each laser? 6. It seems the need for ASF change is originated from the small size of the QC bead. Will it is not much easier if you use a larger QC bead? 7. As you showed with the fluorescence protein example. Is it always needed to have a positive signal (higher than unstain) to adjust ASF for a particular laser? Why can not I just use unstained cells? 8. When you changed ASF for blue laser you used MFI from two pulses (Unstained pulse and stained pulse, stained pulse has much steeper slope). What is the advantage of using both pulses together? To me, it looks like the use of only one is the best.

Lindsay brought me here because i have never seen what flow cytometer looked like and now i do.

great beat

Nice vid. You have a great voice!

✌🏻

Very BD style shutdown process. Modern cytometers ought to do this automatically.

Hi It is not clear how you calculate the complexity index. It is certainly not the summation of the similarity index. The similarity index of BV421 and APC is 0 but the complexity index is 1. The similarity index of BV421 and APC-H7 is 0 but the complexity index is 1.38. This means complexity index do consider some other parameter than similarity index, what is that? If I add PE with BV421 and APC-H7 then still complexity index remains unchanged. Can you please explain that in simple terms?

Poor sound quality. Could you please re-post this with clearly audible sound??

Hello, I have been using tubes for acquisition, but I want to start using plates from now on. Regarding that, is it possible to use a reference group template of a previous experiment created with tubes, and continue acquisition with a plate?