This video series covers the best practices for flow cytometry panel design. In this third step, we will review spillover-spreading error. We will begin by reviewing examples of spread, then show how spread is quantified using the stain index reduction (SIR) and spillover spreading value (SSV). Lastly, we will review the factors that impact spread so you can best minimize its impact on your panel design.
Visit cytekbio.com/blogs/spectrolearn for more helpful information for using the Aurora, Northern Lights, and Aurora CS systems.