Also check article Rapid purification of RNAs using fast performance liquid chromatography (FPLC) Insil Kim,1 Sean A. McKenna,1 2007

Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.

Yes. It is mixture of both Paracetamol and Aceclofenac

So do we have to calculate it or by running the mixture just in UV we get A1 and A2@@analyticaltechniques7088

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Distilled water

What is the appropriate blank?

The solvent in which you dissolve sample will be your blank. It can be any solvent . Solvent should be UV transparent ( It shouldn't absorb UV radiation at the wavelength of analysis).

@@analyticaltechniques7088 Oh I get it now so in this case, distilled water is the best option since it'll have no effect on the absorbance. Would I still have to dissolve the phenol in the distilled water to serve as the blank?

My confusion here is just about the water in the experiment and then the blank.... From what I understand, I'll prepare phenol in cyclohexane and then phenol in water. For my blank, I'll dissolve phenol in an appropriate solvent which will have no effect in the absorbance of phenol and that most suitable solvent is distilled water. Is this the case?

Generally salts of acids and bases are ionizable in nature

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In isocratic separations, analyte retention time can be normalised to retention factor, k, which allows a direct comparison between columns with different dimensions. This is possible due to the constant mobile phase composition used in isocratic methods.Gradient retention factor, k*, also known as average k, can be used to describe the chromatographic conditions. k* requires a range of 2 < k* < 10, similar to that of retention factor, k. If k* is below 2, there is potentially insufficient interaction between the analyte and the stationary phase, whilst above 10, the run times can be excessive, which can decrease laboratory productivity and increase solvent consumption

Column back pressure is back pressure due to Column. As mobile phase passes through Column containing stationary phase of fine particle (3 micron or 5 micron), the pressure is generated.

Silica based column, Cyano column, Amino column

For Chromatography: Remington Volume 1 For Spectroscopy: Pavia, Skoog, William Kemp,

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Before commenting you should know what you are telling. By the way in GC, separation is not based on boiling point. It is based on affinity of samples towards stationary phase and mobile phase( career Gas)

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