Very nice video lec..more important and helpfull..thankyou 🎉
@pharmaseries23425 күн бұрын
How to make 0.002 % phenol solution from phenol liquid
@saikumartallaparthi8090Ай бұрын
Tq lot of learning ponits for hplc Ur video
@user-dn8om4lj6vАй бұрын
❤
@mr.kdschemistry76212 ай бұрын
Where is the collector in TYPE IV dissolution, in the diagram of closed system????
@analyticaltechniques70882 ай бұрын
Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.
@92dahman632 ай бұрын
very good post, i have a correction here, at 14:10 you said that you can separate RNA and DNA with size exclusion chromatography which is not true RNA and DNA can be separated by gel electrophoresis :)
@analyticaltechniques70882 ай бұрын
Also check article Rapid purification of RNAs using fast performance liquid chromatography (FPLC) Insil Kim,1 Sean A. McKenna,1 2007
@analyticaltechniques70882 ай бұрын
Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.
@AmolThakor3 ай бұрын
Nice Explain
@BARATHKUMARK-cp7ll5 ай бұрын
Thanks mam You lecture was very useful for me
@harshadasonawane74455 ай бұрын
what is that sample.. is it mixture
@analyticaltechniques70885 ай бұрын
Yes. It is mixture of both Paracetamol and Aceclofenac
@harshadasonawane74455 ай бұрын
So do we have to calculate it or by running the mixture just in UV we get A1 and A2@@analyticaltechniques7088
@swarnalatha14516 ай бұрын
Excellent explanation thanks 🙏
@analyticaltechniques70886 ай бұрын
Kindly share with relevant people
@ranjitpatil3597 ай бұрын
Excellent💯💯💯
@maheswarikorukonda86888 ай бұрын
I want pdf of calibration in hptlc can u please provide for me...
@andrewstetteh-wayoe74778 ай бұрын
Can I use the sulphanilamide in distilled water as my blank so that I'll check for the effect of low pH from HCl on sulphanilamide and then effect of high pH from NaOH on sulphanilamide
@andrewstetteh-wayoe74778 ай бұрын
Is the water ordinary water or distilled water? Also, what is the appropriate blank?
@analyticaltechniques70888 ай бұрын
Distilled water
@andrewstetteh-wayoe74778 ай бұрын
What is the appropriate blank?
@analyticaltechniques70888 ай бұрын
The solvent in which you dissolve sample will be your blank. It can be any solvent . Solvent should be UV transparent ( It shouldn't absorb UV radiation at the wavelength of analysis).
@andrewstetteh-wayoe74778 ай бұрын
@@analyticaltechniques7088 Oh I get it now so in this case, distilled water is the best option since it'll have no effect on the absorbance. Would I still have to dissolve the phenol in the distilled water to serve as the blank?
@andrewstetteh-wayoe74778 ай бұрын
My confusion here is just about the water in the experiment and then the blank.... From what I understand, I'll prepare phenol in cyclohexane and then phenol in water. For my blank, I'll dissolve phenol in an appropriate solvent which will have no effect in the absorbance of phenol and that most suitable solvent is distilled water. Is this the case?
@FullyLoaded-hg9lf8 ай бұрын
Fantastic
@PharmaMedicinalChemistry20219 ай бұрын
Excellent & highly useful presentation on HPLC, Thank you mam.
@bharatmahajan17539 ай бұрын
Good video for team thank you
@8mariyababbar919 ай бұрын
Thanks alot it really helped
@Monkeylluffy10 ай бұрын
Can i use this vedio for b pharm sub advanced instrumentation technique
@SamarawuIbrahim10 ай бұрын
You are doing great ❤
@krish_krish35411 ай бұрын
36 minutes chiral detector ion detector comes under which property detector bulk or solute
@krish_krish35411 ай бұрын
How do we know that from structure our compound is ionizabale and non ionizabale
@analyticaltechniques708811 ай бұрын
Generally salts of acids and bases are ionizable in nature
@krish_krish35411 ай бұрын
@@analyticaltechniques7088 thank you
@krish_krish35411 ай бұрын
What is the use of retention factor and gradient retention factor
@analyticaltechniques708811 ай бұрын
In isocratic separations, analyte retention time can be normalised to retention factor, k, which allows a direct comparison between columns with different dimensions. This is possible due to the constant mobile phase composition used in isocratic methods.Gradient retention factor, k*, also known as average k, can be used to describe the chromatographic conditions. k* requires a range of 2 < k* < 10, similar to that of retention factor, k. If k* is below 2, there is potentially insufficient interaction between the analyte and the stationary phase, whilst above 10, the run times can be excessive, which can decrease laboratory productivity and increase solvent consumption
@krish_krish35411 ай бұрын
Can you explain what is column back pressure
@analyticaltechniques708811 ай бұрын
Column back pressure is back pressure due to Column. As mobile phase passes through Column containing stationary phase of fine particle (3 micron or 5 micron), the pressure is generated.
@MizanurRahman-jt5vp Жыл бұрын
Thank you
@prashanthvynala6475 Жыл бұрын
excellent lecturing madam
@chinna9412 Жыл бұрын
Tqq so much madam
@user-jy4qu4ie5x Жыл бұрын
Nice explain madam
@ravishwa4575 Жыл бұрын
Superb mam, 🙏
@krishnahire3572 Жыл бұрын
Which are normal phase coloume then
@analyticaltechniques7088 Жыл бұрын
Silica based column, Cyano column, Amino column
@purushothamalakuntla2689 Жыл бұрын
Very thankful madam 💓💓💓💓
@darshitpatel6100 Жыл бұрын
Very rare educational contant available like this on KZfaq so appreciate for this kind of work.
@darshitpatel6100 Жыл бұрын
Awesome 👍
@user-ot8fo9py9t Жыл бұрын
Please suggest few books on analytical techniques.....
@analyticaltechniques7088 Жыл бұрын
For Chromatography: Remington Volume 1 For Spectroscopy: Pavia, Skoog, William Kemp,
@user-ot8fo9py9t Жыл бұрын
@@analyticaltechniques7088 Thank you
@user-ot8fo9py9t Жыл бұрын
Your all lectures are wonderful....seen lot of content on chromatography but nothing equivalent to this lecture series....thank you....
@RahulBangare Жыл бұрын
Totally Good 😃😊
@analyticaltechniques7088 Жыл бұрын
Before commenting you should know what you are telling. By the way in GC, separation is not based on boiling point. It is based on affinity of samples towards stationary phase and mobile phase( career Gas)
@ashishukla89 Жыл бұрын
Which software is it?
@analyticaltechniques7088 Жыл бұрын
ChromNav
@ElizabethGavaya Жыл бұрын
I need pharmaceutical videos plizz that can help mi especially in chemistry
@lokendrajadon4702 Жыл бұрын
Amazing mam Calibration oh HPLC
@zahidakhan1304 Жыл бұрын
Very well explained...thank you ma'am 👍
@adithyaveeroji8099 Жыл бұрын
🙏 U saved me literally❤
@SalmanKhan-sv1up Жыл бұрын
Hi.mam
@muhammadyasir8795 Жыл бұрын
Very nice
@selfugirma9957 Жыл бұрын
Wonderful explanation!
@sobiyak8052 Жыл бұрын
Thank you so much mam
@06_soniyakundnani13 Жыл бұрын
Thanks for nice explanation mam its really helpful