Practical Aspects of HPLC Method Development

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Analytical Techniques

Analytical Techniques

3 жыл бұрын

Пікірлер: 124
@pharmasolutionpakistan7668
@pharmasolutionpakistan7668 3 жыл бұрын
Good job i am a teacher as well as lab instrument trainer i appreciate yu your convying mthd z very good
@dr.jitendrajharbade5221
@dr.jitendrajharbade5221 2 жыл бұрын
Excellent lecture, thank you to all team members.....
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Kindly share with relevant audience
@ojaswighadge7435
@ojaswighadge7435 3 жыл бұрын
Very very excellent Dr. Sonal. Thanks for such a useful piece of information.
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
share among your students
@saikumartallaparthi8090
@saikumartallaparthi8090 6 күн бұрын
Tq lot of learning ponits for hplc Ur video
@parixitprajapati
@parixitprajapati 3 жыл бұрын
Nice video. Keep it up👍🏻
@ranjitpatil359
@ranjitpatil359 6 ай бұрын
Excellent💯💯💯
@PharmaMedicinalChemistry2021
@PharmaMedicinalChemistry2021 8 ай бұрын
Excellent & highly useful presentation on HPLC, Thank you mam.
@SamarawuIbrahim
@SamarawuIbrahim 9 ай бұрын
You are doing great ❤
@zahidakhan1304
@zahidakhan1304 Жыл бұрын
Very well explained...thank you ma'am 👍
@analyticaltechniques7088
@analyticaltechniques7088 Ай бұрын
Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.
@swarnalatha1451
@swarnalatha1451 5 ай бұрын
Excellent explanation thanks 🙏
@analyticaltechniques7088
@analyticaltechniques7088 5 ай бұрын
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@bhargaviyatham6303
@bhargaviyatham6303 2 жыл бұрын
Excellent very easy to understand and who prepared for newly in interview purpose will deffinately help ... Thank u for this vedio. .
@analyticaltechniques7088
@analyticaltechniques7088 2 жыл бұрын
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@BARATHKUMARK-cp7ll
@BARATHKUMARK-cp7ll 4 ай бұрын
Thanks mam You lecture was very useful for me
@purushothamalakuntla2689
@purushothamalakuntla2689 Жыл бұрын
Very thankful madam 💓💓💓💓
@prakashg7066
@prakashg7066 3 жыл бұрын
Thank u so much and ur explaining style is beautiful & easy to learn!
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
share with others
@lawanyapandey1660
@lawanyapandey1660 Жыл бұрын
Thanks mam It was very fruitful for me
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Keep watching
@aniksinha3734
@aniksinha3734 2 жыл бұрын
Excellent presentation of different aspects of HPLC, Thank you mam.
@analyticaltechniques7088
@analyticaltechniques7088 2 жыл бұрын
Thank you
@madhurahegde2603
@madhurahegde2603 3 жыл бұрын
good information...
@manukumarbhandoria2554
@manukumarbhandoria2554 2 жыл бұрын
Thanks a lot mam for the detailed presentation in such a lucid way.
@samirkotkar1513
@samirkotkar1513 2 жыл бұрын
Sanket want to talk same things
@bharatmahajan1753
@bharatmahajan1753 8 ай бұрын
Good video for team thank you
@shribhagwan4539
@shribhagwan4539 2 жыл бұрын
Thanks for sharing valuable information on different aspects of HPLC
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
share with others
@manishbhoye4344
@manishbhoye4344 2 жыл бұрын
Nice lecture on different aspects of HPLC method development.
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Keep watching
@dr.nayanpanchani9015
@dr.nayanpanchani9015 2 жыл бұрын
Very good explanation on method Development of HPLC
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
share with others
@deepanshikhauniyal2408
@deepanshikhauniyal2408 2 жыл бұрын
Thank mam for presenting such knowledge for chromatography..
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Thanks for liking
@ravishwa4575
@ravishwa4575 11 ай бұрын
Superb mam, 🙏
@satishkumarsingh3919
@satishkumarsingh3919 2 жыл бұрын
Excellent lecture.
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
share with others
@indie1751979
@indie1751979 2 жыл бұрын
Very nice lecture on HPLC. Thank you.
@analyticaltechniques7088
@analyticaltechniques7088 2 жыл бұрын
Share the videowith others
@mandepraveen
@mandepraveen 3 жыл бұрын
Good lecture
@FullyLoaded-hg9lf
@FullyLoaded-hg9lf 7 ай бұрын
Fantastic
@jeanniedevereaux5857
@jeanniedevereaux5857 3 жыл бұрын
Thank you
@gaganrathore5455
@gaganrathore5455 2 жыл бұрын
Thankyou ma'am, for HPLC method development explain in easy way 😊
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Keep watching
@prashanthvynala6475
@prashanthvynala6475 11 ай бұрын
excellent lecturing madam
@4fourty242
@4fourty242 Жыл бұрын
Helpful information 👍🏻👍🏻👍🏻👍🏻
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Stay connected
@ashleshaghadge2701
@ashleshaghadge2701 2 жыл бұрын
Salute to mam💐💐
@malharbarot9652
@malharbarot9652 Жыл бұрын
Outstanding presentation 👏
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Kindly share with relevant audience
@vinayakkumbhar725
@vinayakkumbhar725 3 жыл бұрын
Nicely explained and very useful beginners
@analyticaltechniques7088
@analyticaltechniques7088 3 жыл бұрын
Thank you. Share and subscribe. Also watch kzfaq.info/get/bejne/bd1xitmWmavPoZ8.html
@ravirajlandage3580
@ravirajlandage3580 3 жыл бұрын
Excellent PPT and excellent explanation
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
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@chandrasekharm8628
@chandrasekharm8628 Жыл бұрын
Very good information and expline medam
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Share with others..and subscribe my channel
@sobiyak8052
@sobiyak8052 Жыл бұрын
Thank you so much mam
@vimalthakur1602
@vimalthakur1602 Жыл бұрын
Very nice knowledge full points👍
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Thanks for liking
@RahulBangare
@RahulBangare Жыл бұрын
Totally Good 😃😊
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Before commenting you should know what you are telling. By the way in GC, separation is not based on boiling point. It is based on affinity of samples towards stationary phase and mobile phase( career Gas)
@kasich4843
@kasich4843 2 жыл бұрын
Great lecture.keep it up
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Thanks for liking
@PharmaceuticalConcepts_Ojaswi
@PharmaceuticalConcepts_Ojaswi 2 жыл бұрын
Excellent madam.. Very nicely explained..
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Thanks a lot
@06_soniyakundnani13
@06_soniyakundnani13 Жыл бұрын
Thanks for nice explanation mam its really helpful
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Thanks for liking
@yogeshshinde9354
@yogeshshinde9354 3 жыл бұрын
Explanation is best.
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Keep watching
@monashah4079
@monashah4079 2 жыл бұрын
Excellent explanation of HPLC
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
share with others
@eswarbabu6938
@eswarbabu6938 3 жыл бұрын
Excellent lecture madam,,,, thanks🙏..........
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
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@mangammollasrinivas6003
@mangammollasrinivas6003 2 жыл бұрын
Madam please du more videos regarding method development... What are the common problems we are facing while performing method development...
@dorababukanda8703
@dorababukanda8703 3 жыл бұрын
Good
@sonajigaikwad6863
@sonajigaikwad6863 2 жыл бұрын
Very very excellent presentation thanks mam
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
share with others
@santidas1
@santidas1 2 жыл бұрын
nice session on HPLC method development
@analyticaltechniques7088
@analyticaltechniques7088 2 жыл бұрын
Thank you. Share with others
@subhajitsikdar7658
@subhajitsikdar7658 2 жыл бұрын
Excellent lecture
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
share with others
@ramnandaniya7636
@ramnandaniya7636 2 жыл бұрын
Interesting lecture
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Many thanks!
@kirankaarumanchi
@kirankaarumanchi 3 жыл бұрын
Your explaining very nice madam
@analyticaltechniques7088
@analyticaltechniques7088 3 жыл бұрын
Share with your group
@renganayakidharmarajan9257
@renganayakidharmarajan9257 2 жыл бұрын
excellent presentation
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Many thanks
@ganeshchalamala53
@ganeshchalamala53 2 жыл бұрын
Excellent
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Thanks
@dorakondaanilkumar6044
@dorakondaanilkumar6044 2 жыл бұрын
Thank you madam... good explanation...
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Thanks for liking
@dr.s.rajalakshmi5424
@dr.s.rajalakshmi5424 2 жыл бұрын
Nice video
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
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@shailendrakushwaha7266
@shailendrakushwaha7266 2 жыл бұрын
Thanks ma'am
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Most welcome 😊
@isaks1880
@isaks1880 3 жыл бұрын
Very nice mam
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
share with others
@rainbowchemistrysudhakar4913
@rainbowchemistrysudhakar4913 2 жыл бұрын
Super explanation
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Thank you
@sahidulchem
@sahidulchem 2 жыл бұрын
Excellent lecture mam
@analyticaltechniques7088
@analyticaltechniques7088 2 жыл бұрын
Thank you...Share with others
@analyticaltechniques7088
@analyticaltechniques7088 2 жыл бұрын
kzfaq.info/get/bejne/l7OoqqecsNOXmac.html
@anuragtyagi1134
@anuragtyagi1134 2 жыл бұрын
Very good
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
Thanks
@dr.devangr.pandya2169
@dr.devangr.pandya2169 2 жыл бұрын
Very excellent
@analyticaltechniques7088
@analyticaltechniques7088 2 жыл бұрын
Thank you. Share with others
@analyticaltechniques7088
@analyticaltechniques7088 2 жыл бұрын
kzfaq.info/get/bejne/l7OoqqecsNOXmac.html
@krish_krish354
@krish_krish354 9 ай бұрын
36 minutes chiral detector ion detector comes under which property detector bulk or solute
@HariKrishna.vallepu
@HariKrishna.vallepu 3 жыл бұрын
Excellent mam. Can you explain GC
@analyticaltechniques7088
@analyticaltechniques7088 Жыл бұрын
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@92dahman63
@92dahman63 Ай бұрын
very good post, i have a correction here, at 14:10 you said that you can separate RNA and DNA with size exclusion chromatography which is not true RNA and DNA can be separated by gel electrophoresis :)
@analyticaltechniques7088
@analyticaltechniques7088 Ай бұрын
Also check article Rapid purification of RNAs using fast performance liquid chromatography (FPLC) Insil Kim,1 Sean A. McKenna,1 2007
@analyticaltechniques7088
@analyticaltechniques7088 Ай бұрын
Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.
@krish_krish354
@krish_krish354 2 жыл бұрын
Column back pressure increases in short or long column17.48 min
@analyticaltechniques7088
@analyticaltechniques7088 2 жыл бұрын
with longer column , column back pressure increases
@krish_krish354
@krish_krish354 9 ай бұрын
What is the use of retention factor and gradient retention factor
@analyticaltechniques7088
@analyticaltechniques7088 9 ай бұрын
In isocratic separations, analyte retention time can be normalised to retention factor, k, which allows a direct comparison between columns with different dimensions. This is possible due to the constant mobile phase composition used in isocratic methods.Gradient retention factor, k*, also known as average k, can be used to describe the chromatographic conditions. k* requires a range of 2 < k* < 10, similar to that of retention factor, k. If k* is below 2, there is potentially insufficient interaction between the analyte and the stationary phase, whilst above 10, the run times can be excessive, which can decrease laboratory productivity and increase solvent consumption
@krish_krish354
@krish_krish354 9 ай бұрын
How do we know that from structure our compound is ionizabale and non ionizabale
@analyticaltechniques7088
@analyticaltechniques7088 9 ай бұрын
Generally salts of acids and bases are ionizable in nature
@krish_krish354
@krish_krish354 9 ай бұрын
@@analyticaltechniques7088 thank you
@krish_krish354
@krish_krish354 9 ай бұрын
Can you explain what is column back pressure
@analyticaltechniques7088
@analyticaltechniques7088 9 ай бұрын
Column back pressure is back pressure due to Column. As mobile phase passes through Column containing stationary phase of fine particle (3 micron or 5 micron), the pressure is generated.
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