Notes on Southern Blotting- Principle, Procedure and Applications www.biologyexams4u.com/2013/12... Southern Blotting Steps www.biologyexams4u.com/2013/12...
Пікірлер: 44
@arunimaviswanathan43166 жыл бұрын
Very well explained ! Good work
@kavithanair15278 жыл бұрын
very simple and easy to understand...... thank u...
@user-iz2kl3fo9x3 ай бұрын
Thank u so much brother/sir 🥺🙌❣️
@aboutstudies1236 жыл бұрын
Thank you Sir. Very nice explanation
@malv49358 жыл бұрын
Thank you so much! It explained it perfectly.
@sindhusew4 жыл бұрын
S
@amruthaammu15037 жыл бұрын
very useful...and thank u !!!
@sanaariaz68 жыл бұрын
thank you 😊
@priyankabya20923 жыл бұрын
Sir! What are the requisites for this southern blotting practical?
@beingcreative7817 Жыл бұрын
Love from India... Thanks for your efforts..
@biologyexams4u Жыл бұрын
So nice of you..... Love from India too
@161-shubhangiprajapati82 ай бұрын
❤❤❤❤
@TamchrangАй бұрын
It helps me so much thank you
@biologyexams4uАй бұрын
Glad it was helpful... Thank you
@arunabhparkhi20087 жыл бұрын
Thankz...
@sheebakhan60279 жыл бұрын
sound too low to hear improve sound quality
@drsaharabdulkarim89967 жыл бұрын
helped me a lot at least to know an overall procedure and continue my search .....thank you so much sir
@rushabhlovesu8 жыл бұрын
Very helpful...thank you!..
@biologyexams4u8 жыл бұрын
Really appreciate your nice words. Really a motivation to do more.....Thank you so much for the visit and comment
@leonardobuback44089 жыл бұрын
Hello, First of all, nice video. Second, i have a question: I'd like to know how sensible are electroforesis in distinguishing DNA fragments by size. Would I be able to notice any diference between a fragment of 1 kb and a fragment of 2 kb? Thank you
@JC-vi6rc9 жыл бұрын
Leonardo Buback Yes, a 1kb difference in DNA fragments would be easily resolved by gel electrophoresis. I think a 1% gel (1g agarose powder per 100 mL 1X TAE), ran at ~100 volts for 30-45 minutes would work beautifully. You would run a ladder to gauge the sizes of bands based on their migration. I would use the 1kb ladder from New England Biolabs.
@chandinigupta27669 жыл бұрын
How is the nitrocellulose membrane helping in the hybridization? Why is it used? Can't it be done directly on the gel?
@hershcheriyil61482 жыл бұрын
This is a speciall Membranous sheet where molecules can be attached gel can't be used ahead . In southern blotting we find a gene of interest from target dna for which addition of probe is required n then additional process like autoradiography ( incase of radio active probe) where how will you use this gel? If you are familiarize with how initially we loaded the dna you would understand it's not practical and i don't think it's possible for probe to run over gel again.
@sindhusew4 жыл бұрын
Tq
@ghema8602 жыл бұрын
Thank u sir 👍
@biologyexams4u2 жыл бұрын
Hi Hema Most welcome...All the best Just sharing a related video for your reference kzfaq.info/get/bejne/p9CobKycspbDXY0.html Please consider subscribing biologyexams4u. Thank you so much for your support, Stay Safe and Stay Blessed:)
@tanisakarar82862 жыл бұрын
💜💜💜💜💜
@biologyexams4u2 жыл бұрын
Thank you:)
@sujaygowda43582 жыл бұрын
Bro, is colonial hybridization and southern blotting is same or different?
@biologyexams4u2 жыл бұрын
Brother Colony hybridization is used to find a specific DNA within a bacterial colony Southern blotting is used to find a specific DNA fragment in a isolated DNA sample The principle of both procedure is same that is hybridization Thank you:)
@sujaygowda43582 жыл бұрын
@@biologyexams4u tq bro
@marcushooo10 жыл бұрын
this is a great video, very informational. but it would be better if you did the same video in English instead.
@biologyexams4u10 жыл бұрын
Hi marcushoo, Thank you for the visit and comment. I think, language is not a barrier if our intention is to share our knowledge. Hope you got something.....
@Pink_kiwi7079 жыл бұрын
mho I understood
@khushboojaiswal6371 Жыл бұрын
Northan and western blotting ka video nhi bnaye hai kya sir
@biologyexams4u Жыл бұрын
ज़रूर 👍
@khushboojaiswal6371 Жыл бұрын
Thank you sir jaldi bna dijiye please sir 🙏
@ashwaqsalem8601 Жыл бұрын
I want pdf please i have exam
@biologyexams4u Жыл бұрын
www.biologyexams4u.com/2013/12/southern-blotting-principle-procedure.html Hope this may help you. Thank you so much
@redpandameghalaya24666 жыл бұрын
Speak louder please
@biologyexams4u6 жыл бұрын
Hi Chisak Really appreciate your visit and comment. Definitely will work on it.