The Principle of Flow Cytometry and FACS (1- Flow Cytometry)

Рет қаралды 161,147

6 жыл бұрын

This video is an explanation of Flow Cytometry, it contains a full explanation about flow cytometer and data obtaned from it.

Пікірлер: 96

@romelsoyza4160 6 жыл бұрын

Your excellent explanation on how Flow Cytometry works helped me a lot today. Thanks!

@russellmcgahee942 4 жыл бұрын

Clinical Laboratory Engineering Specialist Florida Hospital Orlando, Florida. This was one of the best learning tools I have had the opportunity to view. I highly recommend it to Laboratory Technicians and service engineers alike. Well done. Russell McGahee ISA, CLES

@ramkrishna4033 4 жыл бұрын

Outstanding explanation of flow cytometry. Thank you!

@AA-gl1dr Жыл бұрын

Very clear and understandable. Thank you greatly for your lectures!

@yafitamosi7601 3 жыл бұрын

I am a nursing stutend in my last year. I enjoyed your's explenation a lot!!

@loayk2754 4 жыл бұрын

Thank for the simplicity of explanation

@arminkh7233 6 жыл бұрын

Thank you, for your powerful explanation that make everything easy to learn. Good luck🌹

@FAIZANEAAMIRPIYA-Rh 5 жыл бұрын

kzfaq.info/get/bejne/pdhyqZiLzNm6aIU.html

@jannaarmstrong4048 4 жыл бұрын

Very good job at explaining this procedure!

@esrakayikci934 2 жыл бұрын

I really like to watch this video. I almost understand all the thing that you explained. thank you for this video!

@avijitekumersarkar2064 3 жыл бұрын

Thank you very much for your wonderful explanation that make anyone to understand Flow Cytometry. Good luck.

@zulyc8641 4 жыл бұрын

this made it very clear thank you!

@user-he7ms9po8n 5 жыл бұрын

Thank you so much! This is very useful!!!

@courageidahor 2 жыл бұрын

Excellent explanation!! Thank you very much.

@Drmzzzzzzz 3 жыл бұрын

Great explanation, thanks !

@ernestaguinam2017 4 жыл бұрын

Awesome explanation. Thanks

@blissgr 5 жыл бұрын

Amazing..very helpful video.thank you

@goldenkumar5407 5 жыл бұрын

Thanks for the video on the flow cytometry. You are an amazing teacher

@wheramou Жыл бұрын

Very clear and helpful! Thank you!

@martinjames4293 4 жыл бұрын

Excellent video. Thank you

4 жыл бұрын

Very useful and didactic video. Congrats

@aarefalokhandwala6578 5 жыл бұрын

Very well explained.. Thank u.

@sharb8552 4 жыл бұрын

Thank you so much for helping me out today! :)

@sharb8552 4 жыл бұрын

Now I will watch the video about FACS :)

@Haideroshika 2 жыл бұрын

Thanks for your excellent explanation about flow cytometry

@majidnasrollahi1342 4 жыл бұрын

It was very useful for me. Thanks a lot.

@mayaasrivastava23 Жыл бұрын

Loved the explanation

@auwalgarba7907 Жыл бұрын

Very nice video, this has helped me a lot. Gracias

@mernaelnosiry9672 3 жыл бұрын

Very useful. Thank you ❤️

@mohanrajs9254 2 жыл бұрын

Good Explanation.....Thank you so much

@ngnhunghia 2 жыл бұрын

Very well explained

@anjaliaggarwal2963 2 жыл бұрын

Made it so simple... Thankyou

@princeswasedominic4931 4 жыл бұрын

Very well and clear explained video... thank you ma

@rbkuwar 3 жыл бұрын

very succinct but powerful presentation.. Good job...

@marwaabdalla4676 5 жыл бұрын

Perfect, thanks

@shaioomsyr5922 3 жыл бұрын

Easy explanation I like it thank you

@lisacai8110 4 жыл бұрын

Thank you very much! Very useful and clear for me! I will watch the FACS now!

@omargallow9136 4 жыл бұрын

Thanks for this helpful video it is amazing

@user-qx6nh6sj2s 4 жыл бұрын

Awesome explain thank you son much

@nnpatel44 4 жыл бұрын

Thank you 🙏🏼 Very well explained. Very easy to understand. Keep making more videos like this please for us amateurs scientists 👩‍🔬

@AjithKumar-iu1ri 3 жыл бұрын

Awesome explanation 🥳🥳🥳🥳🥳

@biology-world 4 жыл бұрын

Love you... Mam it was complete 100%

@lets_start_preparing3216 3 жыл бұрын

Nicely explained 😌

@Drsamiullahkhan4353 9 ай бұрын

Great explanation..

@blurryperson2685 3 жыл бұрын

Currently reviewing this for a job interview as a Flow Cytometry Technician

@muhammadashraf-iy1gd 9 ай бұрын

Outstanding

@sasbh1542 4 жыл бұрын

make more videos, you are doing very well. congratulations

@loukikpatil7021 2 жыл бұрын

Thank You!

@fabricgsome Жыл бұрын

Hello, outstanding explanation

@songthanh896 2 жыл бұрын

Thanks so much

@shyamalsingh2900 Жыл бұрын

Nice presentation

@taharyasmine5944 Жыл бұрын

Thank you 🙏🙏

@Mohitthakurofficial 6 жыл бұрын

Would you be uploading Serology/ImmunoChemical Techniques and Biochemical estimation techniques?

@kapereyonah2252 3 жыл бұрын

thankyou so much

@hiroshafernando5416 2 жыл бұрын

Thank you

@lolaabdo5226 4 жыл бұрын

Very useful

@Omar-Khaairy Жыл бұрын

Thanks 👍

@ManojKumar-hm7fh 4 жыл бұрын

Thanks alot.

@sukantamandal8106 4 жыл бұрын

So helpfull for me

@angie565656 3 жыл бұрын

Thank yoooou ! 😁

@mcgrathcarlos7902 5 жыл бұрын

Hi how you measure or equipment calculated hemoglobin and hematocrites

@yousufhaidari6319 2 жыл бұрын

nice bro

@kondapalliabhishek8463 Жыл бұрын

Thank u

@snehadesai4464 5 жыл бұрын

Is it that pc converts light intensity to voltage

@papinandi4975 6 жыл бұрын

Pls pls upload a video on circular dichromism

@chimpalahee 2 жыл бұрын

Very well explained thank!

@mariya5605 4 жыл бұрын

Thanks sis

@Ericx2010 4 жыл бұрын

Quick question for my general understanding? At around 03:00 you say that the greater the cell, the more light will fall on the detector and vice versa for smaller cells. My question is; Shouldn't it be the other way round, since the size of the detector won't change and the light will scatter more in other directions, thus leaving a larger 'shadow' behind on the detector? Would appreciate an answer and thus thank you in advance. However, thanks for the effort and the very nice demonstration on how this works! Stay motivated and curious! =)

@tazarot 2 жыл бұрын

Scattering appears not from the "borders" of the cell as it is in the pictures here, but from different intristic parts of the cell. So, if you have a larger cell, your detector will get scattered signal for longer time, thus the width of the forward signal peak (which is normally refered as FSC-W) wiil be greater than it is for a smaller cell. The height of the forward scattering peak (FSC-H, the maximum amount of light scattered during an event) could not be directly associated with sizes of the cells, but could be a marker which is unique for every type of cells. Normally we want to take in account both parameters (size and "type"), so we use their combination which is an area under the peaks curves (FSC-A).

@iamsurbhigupta77 5 жыл бұрын

So basically forward scattering depend upon size while side scattering depend on density.

@marissafahlberg721 5 жыл бұрын

Forward scatter depends on size and side scatter depends on granularity, or cell complexity, but not density.

@KJKP 6 жыл бұрын

At 4 minutes, does the data go directly to a PC, or is there a logic controller involved?

@beneamery814 6 жыл бұрын

So small molecules will give less FW scatter in comparison to large molecules? How does that work? I always thought it was the other way around; big molecules block more light then small molecules so give less FW scatter. Can u explain it to me pls?

@biomedicalandbiologicalsci4989 6 жыл бұрын

The cells are so small to block the laser beam ... the small cells cause less forward scatter and the large cells cause more scatter (as shown in the video between 2:50 and 3:00) ...

@ssjgold 6 жыл бұрын

It's less "block more light" and instead scatters the light. So as was said before, all of the cells are too small to block the laser beam entirely. But, instead, as they pass through, they change the angle of the light so that it's not going straight, but scattering it. But think of it like a water tap. If you pass a small thing through it (your finger, for example), the flow breaks and spreads out. But if you pass something much larger (your fist, for example), the same thing happens, but to a much larger area. So, a small cell will scatter/spread out the light as it passes through it, but a bigger cell makes a much larger area of light scatter as it passes through. Hopefully that made sense...

@sukantamandal8106 4 жыл бұрын

More video immunofluorescence,emsa,immunodiffusion,immunochemistry,

@kashafseducationalsite363 4 жыл бұрын

how can chromosomes be sorted by flow cytometry..?

@floflyberlin7392 6 жыл бұрын

so far so good ,but where are the erythrocytes?

@biomedicalandbiologicalsci4989 6 жыл бұрын

Hei, you can extract leucocytes from a blood sample in the laboratory ..

@marissafahlberg721 5 жыл бұрын

Usually the red blood cells are lysed and removed before running cells through the flow cytometer. In addition, we often set the threshold of a flow cytometer too high so that RBCs are excluded. If you were to keep the erythrocytes in the sample and set your threshold low enough to see them, they are very small and not granular, so they would show up at the very bottom left corner.

@abdulkareemallan9835 2 жыл бұрын

who i can detect the number of WBCs

@mohitjesani2797 Жыл бұрын

you need to revise it properly

@saramalik5440 4 жыл бұрын

You have amazing clarity with concepts but you need to work on delivery part, it gets to the point where it pisses off humongously

@danielhewing64 5 жыл бұрын

umm which i have aaaaa an example of i i i have aaaa an aaaaaa example of red blood cells........

@Nefglarien 4 жыл бұрын

I think most important is that she explains everything very clearly and it's easy to understand and remember. There is a lot youtubers with better oral skills but their explanations isn't so easy to follow. So I prefer her "aaaaa" that some else's fluency

@thesublimeu1532 4 жыл бұрын

Such aberrated speech...can't focus at all...myself, being a stammerer, I re-record the broken parts of voice over as many times as required...and that is important!

@aishasaad6101 5 жыл бұрын

Your oration is very poor. Try to improve

@nicolasbard4180 3 жыл бұрын

You didn't do the best at explaining, a lot of "uhm"s and "uhhh"s, you were lost for your words half the time. In saying that the graphics is what saved this. I came here for a quick review so I knew what you were trying to say. I'd prepare a script for your next video.