Good video! just a quick comment to clarify that RT-PCR is Reverse Transcription PCR and Real Time PCR is Q-PCR, for Quantitative PCR, to avoid confusion. Thanks for your video! It was helpful
@Biomeducated4 жыл бұрын
Her videos are awesome! That's the reason I've put her in my '10 Biomedical Science Channels to watch' video on my own channel! Just a pity she doesn't upload anymore :( Hope for a revival!
@aparnasrivastava80644 жыл бұрын
Good explanation
@dranshumansahu61144 жыл бұрын
Biomed Master yeah but small blunders.....she need to improve that......and she’ll become best
@mrlawilliamsukwarmachine49043 жыл бұрын
Intetesting!
@rickscheck53303 жыл бұрын
" RT-PCR is Reverse Transcription PCR" That's what I thought. She says "Real Time" So what's the difference?
@manuelmendez93727 жыл бұрын
I came here crying due to I couldn't understand my class today. I left loving RT PCR. Amazing work! Thank you so so muuuuch!
@biomedicalandbiologicalsci49897 жыл бұрын
Thank you for your comment, it makes me happy o hear that :)
@Calbishi15 жыл бұрын
Yes, Manuel, very handy and great video! Will help our QFT team for sure
@Plantshealth5 жыл бұрын
@@biomedicalandbiologicalsci4989 Egyptians are making good videos.
@jsingh2653 жыл бұрын
@@biomedicalandbiologicalsci4989 amazing. Your videos still having big impact.
@antonioassis79816 жыл бұрын
Seriously, your video is totally amazing. What you have shown here, I guarantee you, most people from the field doesn't know. Just one little insignificant correction, cDNA means "complementary DNA" and don't "circle DNA".
@mehwisha.salman55173 жыл бұрын
She said cyclic not circle
@stanleyho80092 жыл бұрын
@@mehwisha.salman5517 still wrong
@stanleyho80092 жыл бұрын
I’m actually surprised… I’m first year university student and following the course biotechnology and society in the Netherlands and this came by real fast that I forgot exactly what is was so I looked it up again. I cannot imagine people in the field at least a lot of them not knowing about this if they specialize in this field,
@reindorfboateng72303 жыл бұрын
This 5 years ago video has provided me with great knowledge and insight. Thank you very much for the clear and detailed explanation
@Tony_Ss-bx2dp4 жыл бұрын
It is an amazing video! thanks a lot for this explanation. Just one correction; cDNA is for "complementary" not for "cyclic" (22:04). Great job!
@yashkarmarkar80564 жыл бұрын
This is the most logical progression for someone who's looking to understand PCR. Really well structured content, big up!
@DM-xq3qg7 жыл бұрын
your channel deserves more views! i could not have done well at university without you
@biomedicalandbiologicalsci49897 жыл бұрын
Thank youu .. this really makes me happy :)
@AqleemAbbas3 жыл бұрын
@@biomedicalandbiologicalsci4989 Hello Madam, Why have you stopped making videos? Continue please
@farshidyekani39573 жыл бұрын
Good presentation .. Just an important point: cDNA is Not cyclic DNA, It's Called Complementary DNA.
@adityatayde983 жыл бұрын
Thanks for correction
@boinualizeh48773 жыл бұрын
Yes👍
@archanar96762 жыл бұрын
Good presentation
@naveedahmed85442 жыл бұрын
@@boinualizeh4877 good
@educationinfo5080 Жыл бұрын
Means this cDNA undergoes this cyclic reaction so one can say.
@dryounisrather78552 жыл бұрын
i am not a molecular biologist but i can follow and understand your lecture easily, now i can interpret the COVID19 RT-PCR diagnostic result just looking at the curves and figuring out CT value quite easily, thanks and keep sharing knowledge
@selinunsalserbes45874 жыл бұрын
A correction: the reason of becoming non-exponential phase means there is no more reaction components anymore ( dna pol, dntps etc. run out). The explanation of becoming non-exponential phase is not dna polimerase becomes inactive. By the way, that is a great video, thank you!
@Mprince6588 Жыл бұрын
dna pol running out? can an enzyme run out that way? my course teacher said two reasons for the non-exponential phase- 1. gradual thermal inactivation of DNA pol after each denaturation step 2. reduced efficiency of primer annealing (due to increasing competition from the template)
@austinmiles58186 жыл бұрын
This is absolutely incredible. Just what I needed for a presentation next week.
@biomedicalandbiologicalsci49896 жыл бұрын
HAHAHA, very good. Hope you put my channel as a reference :p
@VLAREZENDE19834 жыл бұрын
Biomedical and Biological Sciences I hope too! Very good informations in you video!
@monaalbalawi64702 жыл бұрын
@@biomedicalandbiologicalsci4989 can i have this presentation as a file?
@munerhd60953 жыл бұрын
Omg thank you soooooo much so much 😭 do you know how many times i tried to understand and looking for videos seeking god to understand this pain in the neck machine?!!! You’re awesome I finally understand it again thank you so much my savior 🥺💕
@danial170114 жыл бұрын
I have to admit, pretty impressive ability to comunicate, you really made it cristal clear despite english not being your first language. Thank you very much!
@abhayiyer88645 жыл бұрын
U explain well. I didn't understand RT PCR for my course. Now I understand it. U have great teaching talent.
@cyrtcheu20523 жыл бұрын
This video is very good, very absorbing, you explained this methods simply and precisely. The only thing I can say is that SYBR green instead of Cyber green. It's very interesting.
@adlesal24 Жыл бұрын
You are magnificent. You do not leave any point without detailed explanation. God blessings ❤
@Zweileiter6 жыл бұрын
great video! I already have a Master in Biologie, but never was interested so much in molecular biologie and I totaly get everything about qPCR now! Thanks!!!
@enricocalvanese82236 жыл бұрын
I wish you had more views. You are such a good lecturer and I am really grateful for your time in uploading these videos!
@nandinipriyathangavel82934 жыл бұрын
Thank you madam for your awesome explanation....first I didn't understand the rt PCR before now I'm clear in this topic ...once again thank you madam
@hanazahra10143 жыл бұрын
The doctor said that my parent's CT value needs to be at certain number for them to be able to leave the hospital. I have no idea what it was all about. I came here without any medical knowledge, but your explanation in the video is very good and clear. I don't really understand the biological details but i get the concept of how it works. Thankyou thankyou!!!
@S.Ghosh_2212 жыл бұрын
CT value is the threshold value of fluorescence emitted. More than the CT value means the "gene of interest" (pathogenic gene of virus) has been ascertained. If it's less or nil, then the individual may clinically be diagnosed as "free of the viral infection/disease "
@ilaydademir2284 жыл бұрын
all mechanisms explained clearly! no insignificant details, just the main points, thank you : )
@onzimadaniel3747 Жыл бұрын
Haven't seen such a complete and educative video!!! Thank you.
@Sturdy_Penguin5 жыл бұрын
This is such a helpful video. Thank you so much for it. The only thing I noticed, was the use of "probability" instead of "possibility" at 17:38, where "probability" means "very likely", in this context. English is weird, sometimes, I know. But right. Your video was extremely helpful and the concept of rtPCR is very interesting. I'm glad it's so simple, too, as I have to be able to do it really soon.
@amitkhatti68076 жыл бұрын
Your lecture clarifies many doubts and basics of PCR for me. I highly obliged to your efforts. Still, I have couple of queries. 1. What is actual meaning of Ct value and delta Ct? 2. Many a time, Why we use RNA sample to synthesize c-DNA instead of direct DNA sample for RT-PCR?
@sameirbasnet31022 жыл бұрын
For your second query, for instance in case of covid 19 virus detection we have to use reverse transcriptase pcr because it is a rna virus. So it all depends on what type of nucleic acid you won't to detect
@tianecheverry41233 жыл бұрын
A really helpful video but I think that it is SYBR Green not Cyber green, it really helps me to clarify some doubts that I had thank you so much, by the last qPCR: IS REAL-TIME PCR and RT-PCR: is REVERSE TRANSCRIPTION PCR.
@allisonsmith51155 жыл бұрын
Wow started doing qPCR two days ago and I don’t really know what I am doing but this video really clarified a lot. Thanks. You should make a video about analyzing qPCR data using a reference gene along with a target gene. Would really appreciate that!
@stianborg-stoveland6616 жыл бұрын
Thank you! I needed to refresh this topic, and you covered it all, good job!
@Jose892774 жыл бұрын
an important component for the reaction preparation was missing. The salt buffer. Without optimal pH-value and corresponding divalent cations the polymerase would not work properly at all
@anthonyvillarama68065 жыл бұрын
Awesome. Your presentation and explanation are clear. The best lecture out there. Keep your lectures coming.
@sametcirik42783 жыл бұрын
Finally I could understand the key information thanks to this video after days of another mixed incomprehensible video. Please keep doing this. Congr. 👏👏👏
@jimfarey5 жыл бұрын
Watched this with my girlfriend, thoroughly enjoyed it. She's doing PhD genetics, I was a biochemist 15 years ago. Can I just add some constructive criticism on the language clarity front: "quencher" is pronounced "kwencher", as in "to quench", like when you're thirsty. As an Englishman, I feel I should apologise for our impossible language, your English is very very good!! Also, parent ssDNA is not copied by Taq polymerase/TaqMan, it is extended. The slide makes sense, just not the word "copy" in this context. Keep up the fantastic educational work! I learned something today :)
@drmarium5 жыл бұрын
Its your* English. Not "you're" English :)
@jimfarey5 жыл бұрын
@@drmarium correct. It's also "it's", not possessive "its". :)
@benjaminsulemankurya66643 жыл бұрын
You are an excellent tutor, very clear message. I learnt things I could not understand from other videos.
@prembobby1122 жыл бұрын
More Useful video for Molecular biochemistry Research work...Thank you mam for your video ..
@JQPOINT4 жыл бұрын
Your explanation is so succinct and understandable even for a non-scientist like me!
@trantuantu49063 жыл бұрын
This video provides an easy way to understand RT-PCR and real time PCR. Thanks! Hope you can provide more video, and many people can access this page.
@sandalirashmi81952 жыл бұрын
I dnt know how to say thank you.... i have no words.....😥😥😥 you are amazing...and this is a wonderful video, i watched more videos of you, thank you sooo much n god bless you♥️♥️♥️
@erfanhatami70405 жыл бұрын
this is the first time i got what rt-pcr is.thanks a lot
@thummalaharish55884 жыл бұрын
Nice effort in giving information to the beginners and even the experts. Thanks for the video
@qaisarsbiology52673 жыл бұрын
Thank you so much miss for a such a useful and simple explanation. 15:34 you mentioned the annealing and extension occur in 3 to 5' direction while hitting the fluorescence. I think this process is from 5 to 3" but tell us about the position of the probe in case of 5 to 3. thank you so much we really appreciate your work mam. This is the first video I have seen with such an amazing animated explanation.
@Analysis-Zone2 жыл бұрын
This what i am looking for. Thank you for your effort and nice presentation...
@terrie0017 жыл бұрын
Thanks for the video, I have several questions - I just got a job in the Molecular Hematology lab and needs to learn PCRs in details. Why would someone wants to do real time PCR, like why do you need to know how much DNA is made at a particular time, wouldn't the final measure after - say an hour would be sufficient? So the purpose of the reverse transcription PCR is to measure gene expression. The reason you do RT rather than just PCR is because you want to measure the mRNA (post transcription) rather than DNA (pre transcription - which doesn't tell you if the gene will be transcribed or not), right? Lastly, my lab is really concern about contamination. They even have separate room for Pre-PCR and Post-PCR. They do not allow anything, and I mean ANYTHING, including cell phone or pipette tips from the Post go to the Pre room. Like it can contaminate through air or something? I've never done any PCR before, all I learn is from textbooks.
@miangulhilal68586 жыл бұрын
Outstanding job very informative and easy explanation of difficult thoughts many thanks for your knowledge sharing
@Molecular_Machine6 жыл бұрын
Another wonderful, informative and concise video, as always. Keep going
@irinasegovia49005 жыл бұрын
Clear and simple explanation. Thank you and keep up the great videos.
@mebriezemene92506 жыл бұрын
Thank you very much It is a nice video with a great explanation. It is helpful to me
@ahmedel-wakeel93657 жыл бұрын
Great work :) We need some videos about Flow Cytometer :)
@oluwashinaabiodun36677 жыл бұрын
well explained in the simplest form ever! Kudos
@shariquemf1293 жыл бұрын
The way u try to make us understand is just lovely.. thanks for the video and explaination 🙏🙏❤️
@Gigis11113 жыл бұрын
Well done. The best explanation I could find!
@rubaithasan89115 жыл бұрын
EXCELLENT .......... very easy to understand..........good job
@ponrajarajan4 жыл бұрын
Clarity in ur presentation. I enjoyed it.
@SaraTahaFPTU2 жыл бұрын
Thank you for the very informative video. Easy to understand and to listen to your English accent. and very great presentation skills. Thanks a lot
@drsaimairam157 жыл бұрын
no doubt its an excellent presentation about RT-PCR
@kli78673 жыл бұрын
Amazing video. It is very detailed. was an undergrad, i didnot know the difference between cyber green and taqman. And then in conferences, I didnot understand why Ct in everyone's y axis.
@GamingAmbienceLive3 жыл бұрын
Very good video you did an amazing job simple and doesn’t miss important details, but doesn’t go into an unnecessary amount of detail.
@mehwisha.salman55173 жыл бұрын
You are amazing it took me years to understand ft pcr. But you did it in few minutes
@AhmedSaad-om9vf4 жыл бұрын
Thank you for this amazing video , really i gain great knowledge about qpcr this due to you amazing lecture , i am looking forward to more lessons
@1stPrimadonna2 жыл бұрын
Thank you very much for this video ! You explian very clear and easy to follow. Hope you keep doing this
@poojautekar14464 жыл бұрын
Great explanation... And significant points covered... Keep uploading such videos.. looking forward to it...
@rahi007biologytomicrobiolo53 жыл бұрын
Very well explained, will be helpfull to understand the basics of RT PCR, Thank you
@theivun5 жыл бұрын
You are the Bioanalysis queen!
@mahdisalh71794 жыл бұрын
Thank you very much Today I understood real time PCR ( nice and simplified explanation) thank you so much
@guglielmovesco6764 жыл бұрын
Thank you very much for this video! This is a very clear explanation. One question: could the non exponential plateau phase also due to the fact that all of fluorophore molecules are separated from their quenchers?
@ibrarahmed37616 жыл бұрын
Very good explanation of the process in general. Just a comment that the plateau phase or non-exponential phase is not due to inactivation of DNA polymerase but due to the concentration of the fluorescent dye reaching beyond maximum detection limit. Say for example, you can lit a dark room, and the light will illuminate the room. Keep on increasing the number of lights, and the room will glow more and more until it will reach a saturation point beyond which no increase in light intensity will be visible even by doubling the number of lights. So if the room reaches at full saturation point with 50 lights, increasing the number of lights to 100 or 500 will have no more effects.
@audreyvanya55204 жыл бұрын
Thank you for such a simple and clear explanation between these two assays.
@sihaogao6615 жыл бұрын
Thank you. Your explanation is very clear and the slides you made are awesome.
@priyankanaik19454 жыл бұрын
The amplification does not reach a plateau because the activity of polymerase decreases. It becomes stationery because the dNTPs and other building blocks of DNA get used up and there is nothing left to synthesize the DNA with.
@deveshmishra4255 жыл бұрын
Good Job. The Detector name is SYBR green, not Cyber Green
@aracelyabarcainostroza51907 жыл бұрын
Thank you so much!!! it´s a really good explanation :)
@biomedicalandbiologicalsci49897 жыл бұрын
Thank you for the nice comment :)
@sagarvemula68005 жыл бұрын
Your step by step explanation is excellent Thank you
@manthansurkar4713 жыл бұрын
Nicely Explained, thanks!!
@Thawanratify3 жыл бұрын
Hi! I’m so proud of myself because I understand that your explain! Very very thank you!😂😂😂
@swedd23 жыл бұрын
This is such an informative video! Very useful for a graduate course I'm considering reattempting. Cheers
@SouvikMukherjee727 жыл бұрын
Nice one! Can you please explain about how the melt curve looks like for NTC? and How Ct value is used to determine the amount of gene vis-a-vis housekeeping gene
@biomedicalandbiologicalsci49896 жыл бұрын
The earlier the CT is (the earlier the amplification curve crosses the threshold line), the larger is the amount of gene at the beginning of the experiment ... you do not compare the gene of interest with the housekeeping gene, you compare the CT of the gene of interest in different samples. The housekeeping gene is a gene that is expressed similarly in all the samples so we are sure that all the samples exist in the same amount. In other words, the CT of the housekeeping gene should be the same in all the samples, otherwise, your experiment is not precise. I hope I answered your question ...
@LuizFernando-le3vh3 жыл бұрын
Amazing! I am from Brazil and your explanation does an accessible language (I do not speak English very well). Congratulations on your class. (I use google translate legend kkkk)
@abubakarsaidumshelia5504 жыл бұрын
This is Apt and Well Explained. Many Thanks.
@hamzausman47294 жыл бұрын
Your method of teaching is very good...please give a lecture on somaclonal variation
@luain.muhammad5 жыл бұрын
It was really very beautiful video and also your accent was so nice. Thanks a lot.
@dembrowi_n_c.51115 жыл бұрын
Should we tell her it's SYBR green not Cyber green?
@TangkamCornerUK4 жыл бұрын
Indeed bro.
@imbrother14 жыл бұрын
cyber green sounds cooler.
@edwingreen64393 жыл бұрын
Yes
@ahmedabdelhadi55973 жыл бұрын
also cDNA it’s a complementary DNA not cyclic DNA
@Padmashrinaren2 жыл бұрын
This was such a life saver 🤧🤧❤❤❤🤧🤧
@medicineman7863 жыл бұрын
You are the best!
@noorulain73025 жыл бұрын
Good video really, love from Paklistan...........
@isaacclark98863 жыл бұрын
Really awesome video. Super helpful in clarifying this concept for me. Thank you!
@pritichristian26732 жыл бұрын
Very detailed explanation and vedio. Thanks
@LuisGalindo06 жыл бұрын
Hello and congratulations on your videos. I am very pleased with the amount of detail you were able to provide in such short time. Do you have plans to make a similar video about digital PCR? Greetings
@noornoor-ic4rg7 жыл бұрын
Great simplified explanation...Thank you
@biomedicalandbiologicalsci49897 жыл бұрын
welcome :)
@drashnatmou8623 жыл бұрын
Really helpful for refreshing the whole thing... Thank u so much...
@fauziaazzahra85785 жыл бұрын
Thank you so much, it helps me alot to understand about pcr.. You explained it well so it easier to understand 👍
@omaramin5834 жыл бұрын
Amaaazing work .. thank you very much ..keep it up
@jc-tu6pg5 жыл бұрын
the way you say "quencher" is really cute
@yaqoobkhan84152 жыл бұрын
Informative video keep it up!
@mr.croninsvideos52024 жыл бұрын
Great video! I do have a question, though (and if anyone can answer this, it would be helpful). At 21:20, you mentioned that you include RNAse in the mixture within the PCR tube. I understand that its purpose is to degrade the RNA transcript after it has been reverse transcribed into cDNA, but what is to prevent RNAse from degrading the RNA transcript before reverse transcriptase has had the opportunity to reverse transcribe it?
@NeelamnishanthreddyReddy4 ай бұрын
here we are using RNase H it cleaves only the RNA-DNA hybrid then you get cDNA right
@khinpyae83565 жыл бұрын
It would be a lot better if you finish your sentences and have fewer pauses. It is difficult to stay focused when you have lots of half sentences and pauses. Other than that, the explanation is top notch. (Y) Thank you!
@muhammadhafizsulaiman71633 жыл бұрын
You explain it well friend. Thank you so much.
@jishabose86822 жыл бұрын
Excellent presentation
@moazal-ghamdi90175 жыл бұрын
Thank you so much. The presentation was so clear. Keep up the good work.
@rojfarhan96997 жыл бұрын
thank you for this explanation that was great yea i have some difficulty when i want to know the meaning and for what we can use the the real time pcr analysis section like RN delt rn and RQ ...... so on
@carolinec.picoli68495 жыл бұрын
Congratulations on the video, I found it well didactic.
@mahboobhossain77494 жыл бұрын
This video cleared the principle of rtPCR
@mailaralingams78964 жыл бұрын
much informative and loved the way you make things much easier... here go I subscribed .. tqs keep on doing this work