Good video! just a quick comment to clarify that RT-PCR is Reverse Transcription PCR and Real Time PCR is Q-PCR, for Quantitative PCR, to avoid confusion. Thanks for your video! It was helpful

I came here crying due to I couldn't understand my class today. I left loving RT PCR. Amazing work! Thank you so so muuuuch!

Seriously, your video is totally amazing. What you have shown here, I guarantee you, most people from the field doesn't know. Just one little insignificant correction, cDNA means "complementary DNA" and don't "circle DNA".

This 5 years ago video has provided me with great knowledge and insight. Thank you very much for the clear and detailed explanation

It is an amazing video! thanks a lot for this explanation. Just one correction; cDNA is for "complementary" not for "cyclic" (22:04). Great job!

This is the most logical progression for someone who's looking to understand PCR. Really well structured content, big up!

your channel deserves more views! i could not have done well at university without you

Good presentation .. Just an important point: cDNA is Not cyclic DNA, It's Called Complementary DNA.

i am not a molecular biologist but i can follow and understand your lecture easily, now i can interpret the COVID19 RT-PCR diagnostic result just looking at the curves and figuring out CT value quite easily, thanks and keep sharing knowledge

A correction: the reason of becoming non-exponential phase means there is no more reaction components anymore ( dna pol, dntps etc. run out). The explanation of becoming non-exponential phase is not dna polimerase becomes inactive. By the way, that is a great video, thank you!

This is absolutely incredible. Just what I needed for a presentation next week.

Omg thank you soooooo much so much 😭 do you know how many times i tried to understand and looking for videos seeking god to understand this pain in the neck machine?!!! You’re awesome I finally understand it again thank you so much my savior 🥺💕

I have to admit, pretty impressive ability to comunicate, you really made it cristal clear despite english not being your first language. Thank you very much!

U explain well. I didn't understand RT PCR for my course. Now I understand it. U have great teaching talent.

This video is very good, very absorbing, you explained this methods simply and precisely. The only thing I can say is that SYBR green instead of Cyber green. It's very interesting.

You are magnificent. You do not leave any point without detailed explanation. God blessings ❤

great video! I already have a Master in Biologie, but never was interested so much in molecular biologie and I totaly get everything about qPCR now! Thanks!!!

I wish you had more views. You are such a good lecturer and I am really grateful for your time in uploading these videos!

Thank you madam for your awesome explanation....first I didn't understand the rt PCR before now I'm clear in this topic ...once again thank you madam

The doctor said that my parent's CT value needs to be at certain number for them to be able to leave the hospital. I have no idea what it was all about. I came here without any medical knowledge, but your explanation in the video is very good and clear. I don't really understand the biological details but i get the concept of how it works. Thankyou thankyou!!!

all mechanisms explained clearly! no insignificant details, just the main points, thank you : )

Haven't seen such a complete and educative video!!! Thank you.

This is such a helpful video. Thank you so much for it. The only thing I noticed, was the use of "probability" instead of "possibility" at 17:38, where "probability" means "very likely", in this context. English is weird, sometimes, I know. But right. Your video was extremely helpful and the concept of rtPCR is very interesting. I'm glad it's so simple, too, as I have to be able to do it really soon.

Your lecture clarifies many doubts and basics of PCR for me. I highly obliged to your efforts. Still, I have couple of queries. 1. What is actual meaning of Ct value and delta Ct? 2. Many a time, Why we use RNA sample to synthesize c-DNA instead of direct DNA sample for RT-PCR?

A really helpful video but I think that it is SYBR Green not Cyber green, it really helps me to clarify some doubts that I had thank you so much, by the last qPCR: IS REAL-TIME PCR and RT-PCR: is REVERSE TRANSCRIPTION PCR.

Wow started doing qPCR two days ago and I don’t really know what I am doing but this video really clarified a lot. Thanks. You should make a video about analyzing qPCR data using a reference gene along with a target gene. Would really appreciate that!

Thank you! I needed to refresh this topic, and you covered it all, good job!

an important component for the reaction preparation was missing. The salt buffer. Without optimal pH-value and corresponding divalent cations the polymerase would not work properly at all

Awesome. Your presentation and explanation are clear. The best lecture out there. Keep your lectures coming.

Finally I could understand the key information thanks to this video after days of another mixed incomprehensible video. Please keep doing this. Congr. 👏👏👏

Watched this with my girlfriend, thoroughly enjoyed it. She's doing PhD genetics, I was a biochemist 15 years ago. Can I just add some constructive criticism on the language clarity front: "quencher" is pronounced "kwencher", as in "to quench", like when you're thirsty. As an Englishman, I feel I should apologise for our impossible language, your English is very very good!! Also, parent ssDNA is not copied by Taq polymerase/TaqMan, it is extended. The slide makes sense, just not the word "copy" in this context. Keep up the fantastic educational work! I learned something today :)

You are an excellent tutor, very clear message. I learnt things I could not understand from other videos.

More Useful video for Molecular biochemistry Research work...Thank you mam for your video ..

Your explanation is so succinct and understandable even for a non-scientist like me!

This video provides an easy way to understand RT-PCR and real time PCR. Thanks! Hope you can provide more video, and many people can access this page.

I dnt know how to say thank you.... i have no words.....😥😥😥 you are amazing...and this is a wonderful video, i watched more videos of you, thank you sooo much n god bless you♥️♥️♥️

this is the first time i got what rt-pcr is.thanks a lot

Nice effort in giving information to the beginners and even the experts. Thanks for the video

Thank you so much miss for a such a useful and simple explanation. 15:34 you mentioned the annealing and extension occur in 3 to 5' direction while hitting the fluorescence. I think this process is from 5 to 3" but tell us about the position of the probe in case of 5 to 3. thank you so much we really appreciate your work mam. This is the first video I have seen with such an amazing animated explanation.

This what i am looking for. Thank you for your effort and nice presentation...

Thanks for the video, I have several questions - I just got a job in the Molecular Hematology lab and needs to learn PCRs in details. Why would someone wants to do real time PCR, like why do you need to know how much DNA is made at a particular time, wouldn't the final measure after - say an hour would be sufficient? So the purpose of the reverse transcription PCR is to measure gene expression. The reason you do RT rather than just PCR is because you want to measure the mRNA (post transcription) rather than DNA (pre transcription - which doesn't tell you if the gene will be transcribed or not), right? Lastly, my lab is really concern about contamination. They even have separate room for Pre-PCR and Post-PCR. They do not allow anything, and I mean ANYTHING, including cell phone or pipette tips from the Post go to the Pre room. Like it can contaminate through air or something? I've never done any PCR before, all I learn is from textbooks.

Outstanding job very informative and easy explanation of difficult thoughts many thanks for your knowledge sharing

Another wonderful, informative and concise video, as always. Keep going

Clear and simple explanation. Thank you and keep up the great videos.

Thank you very much It is a nice video with a great explanation. It is helpful to me

Great work :) We need some videos about Flow Cytometer :)

well explained in the simplest form ever! Kudos

The way u try to make us understand is just lovely.. thanks for the video and explaination 🙏🙏❤️

Well done. The best explanation I could find!

EXCELLENT .......... very easy to understand..........good job

Clarity in ur presentation. I enjoyed it.

Thank you for the very informative video. Easy to understand and to listen to your English accent. and very great presentation skills. Thanks a lot

no doubt its an excellent presentation about RT-PCR

Amazing video. It is very detailed. was an undergrad, i didnot know the difference between cyber green and taqman. And then in conferences, I didnot understand why Ct in everyone's y axis.

Very good video you did an amazing job simple and doesn’t miss important details, but doesn’t go into an unnecessary amount of detail.

You are amazing it took me years to understand ft pcr. But you did it in few minutes

Thank you for this amazing video , really i gain great knowledge about qpcr this due to you amazing lecture , i am looking forward to more lessons

Thank you very much for this video ! You explian very clear and easy to follow. Hope you keep doing this

Great explanation... And significant points covered... Keep uploading such videos.. looking forward to it...

Very well explained, will be helpfull to understand the basics of RT PCR, Thank you

You are the Bioanalysis queen!

Thank you very much Today I understood real time PCR ( nice and simplified explanation) thank you so much

Thank you very much for this video! This is a very clear explanation. One question: could the non exponential plateau phase also due to the fact that all of fluorophore molecules are separated from their quenchers?

Very good explanation of the process in general. Just a comment that the plateau phase or non-exponential phase is not due to inactivation of DNA polymerase but due to the concentration of the fluorescent dye reaching beyond maximum detection limit. Say for example, you can lit a dark room, and the light will illuminate the room. Keep on increasing the number of lights, and the room will glow more and more until it will reach a saturation point beyond which no increase in light intensity will be visible even by doubling the number of lights. So if the room reaches at full saturation point with 50 lights, increasing the number of lights to 100 or 500 will have no more effects.

Thank you for such a simple and clear explanation between these two assays.

Thank you. Your explanation is very clear and the slides you made are awesome.

The amplification does not reach a plateau because the activity of polymerase decreases. It becomes stationery because the dNTPs and other building blocks of DNA get used up and there is nothing left to synthesize the DNA with.

Good Job. The Detector name is SYBR green, not Cyber Green

Thank you so much!!! it´s a really good explanation :)

Your step by step explanation is excellent Thank you

Nicely Explained, thanks!!

Hi! I’m so proud of myself because I understand that your explain! Very very thank you!😂😂😂

This is such an informative video! Very useful for a graduate course I'm considering reattempting. Cheers

Nice one! Can you please explain about how the melt curve looks like for NTC? and How Ct value is used to determine the amount of gene vis-a-vis housekeeping gene

Amazing! I am from Brazil and your explanation does an accessible language (I do not speak English very well). Congratulations on your class. (I use google translate legend kkkk)

This is Apt and Well Explained. Many Thanks.

Your method of teaching is very good...please give a lecture on somaclonal variation

It was really very beautiful video and also your accent was so nice. Thanks a lot.

Should we tell her it's SYBR green not Cyber green?

This was such a life saver 🤧🤧❤❤❤🤧🤧

You are the best!

Good video really, love from Paklistan...........

Really awesome video. Super helpful in clarifying this concept for me. Thank you!

Very detailed explanation and vedio. Thanks

Hello and congratulations on your videos. I am very pleased with the amount of detail you were able to provide in such short time. Do you have plans to make a similar video about digital PCR? Greetings

Great simplified explanation...Thank you

Really helpful for refreshing the whole thing... Thank u so much...

Thank you so much, it helps me alot to understand about pcr.. You explained it well so it easier to understand 👍

Amaaazing work .. thank you very much ..keep it up

the way you say "quencher" is really cute

Informative video keep it up!

Great video! I do have a question, though (and if anyone can answer this, it would be helpful). At 21:20, you mentioned that you include RNAse in the mixture within the PCR tube. I understand that its purpose is to degrade the RNA transcript after it has been reverse transcribed into cDNA, but what is to prevent RNAse from degrading the RNA transcript before reverse transcriptase has had the opportunity to reverse transcribe it?

It would be a lot better if you finish your sentences and have fewer pauses. It is difficult to stay focused when you have lots of half sentences and pauses. Other than that, the explanation is top notch. (Y) Thank you!

You explain it well friend. Thank you so much.

Excellent presentation

Thank you so much. The presentation was so clear. Keep up the good work.

thank you for this explanation that was great yea i have some difficulty when i want to know the meaning and for what we can use the the real time pcr analysis section like RN delt rn and RQ ...... so on

Congratulations on the video, I found it well didactic.

This video cleared the principle of rtPCR

much informative and loved the way you make things much easier... here go I subscribed .. tqs keep on doing this work