Cactus Tissue Culture: Propagation Secrets Revealed by Lab Director Francisco!

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Cactus Tissue Culture: Propagation Secrets Revealed by Lab Director Francisco!

Рет қаралды 7,381

Plant Cell Technology

Күн бұрын

Пікірлер: 66

@TheDuckofDoom. Жыл бұрын

On the nopales style cactus you can remove a whole pad, pre-disinfect the whole pad, then cut it into nodes with a scalpel, then do final sterilizing of the explant nodes. This does not require special tools and is less likely to cause infection to the parent plant and explants.

@PlantCellTechnology Жыл бұрын

Thanks for sharing your knowledge with us. are you into cactus tissue culture?

@RyanHasemeier-yl4yx 3 күн бұрын

Brilliant...using reusable, washable glass (presumably cheap initial cost) mason jars, love it!!!

@PollenSax Жыл бұрын

That saves alot of time and energy knowing distilled water is sterile unopened. Awesome.

@Enochulate88 Жыл бұрын

Uncle Ben's ready rice is pre sterilized and also straw pellets are already pasteurized. Grow 🍄 I can help

@PollenSax Жыл бұрын

@MyCOWAVE〰SHROOMS I'm past the mushrooms. I'm into plant tc now, thanks.

@PlantCellTechnology Жыл бұрын

@@PollenSax Great, that you are in tissue culture now! So, you working on which plants?

@Jecht1221 Жыл бұрын

I'd love to see Echeveria and sempervivums. No one has those on youtube. Also update videos on plants are cool to see. Looking forward to seeing how these cactus do. Thank you.

@PlantCellTechnology Жыл бұрын

Thanks! We are trying our best to bring more educational videos for you. so, stay tuned!

@TomHutchinson5 2 ай бұрын

Thank you. I hope to try this. Cactus are great. Eventually I would like to figure out how to do tissue culture on Japanese maple.

@opalnoir 6 ай бұрын

Great video - thank you 🌵

@PlantCellTechnology 6 ай бұрын

Happy to know that it was helpful.

@gabrielbronsztein9467 Жыл бұрын

Thanks for the awesome videos

@PlantCellTechnology Жыл бұрын

Thanks!!

@user-ho1dt3qr2y 10 ай бұрын

plsease show some thing on Dieffenbachia

@PlantCellTechnology 10 ай бұрын

We have some videos lined up. We'll let you know if we get our hands on the plant to work with.

@deonbuys3363 2 ай бұрын

What a wonderful video! Would this protocol also work on Schlumbergera? Or is it too soft to be treated in the same way as other cacti?

@PlantCellTechnology 2 ай бұрын

It would also work for Christmas cactus.

@carolbrinkley6189 5 ай бұрын

I have a Thanksgiving cactus that is a very small plant. I've had it for 2 months, it's not growing. The leaves are a little floppy. Would I be wasting my time to try tissue culture on one of these leaves? Do you think it might grow in tissue culture even if the leaves aren't great?

@tunstallgeo Жыл бұрын

Great video, m8ght gove this a try.

@PlantCellTechnology Жыл бұрын

Thanks! Stay tuned for many such videos!

@opipico9144 8 ай бұрын

Salvia Divinorum please

@bloodyfluffybunny7411 9 ай бұрын

could you possibly do one about citrus tissue culture ive been eager to start with citrus but am hesitating because of doubt about me understanding the process or what parts are best to do tissue culture with also if anyone in the comment has good info on this pls help me out great videos btw keep doing what you're doing

@PlantCellTechnology 9 ай бұрын

Sure! We will do it as we get our hands on the plant. Currently, we are experimenting with many plants in our lab, including cactus, carnivorous plants, and a range of houseplants.

@theindustriousant8280 9 ай бұрын

@PlantCellTechnology update on cactus cultures?

@PlantCellTechnology 9 ай бұрын

They are doing well in our lab. With our TC masterclasses, we didn't get a chance to shoot an update video.

@robertl6711 Ай бұрын

Looking for an alocasia video

@vjsucculents10 11 ай бұрын

Pls have an update to this

@PlantCellTechnology 11 ай бұрын

We will convey this to Francisco. We're sure he is trying to bring as innovative and useful videos as he can. Thanks for your patience!

@davidarcila3459 Жыл бұрын

Hi Francisco, amazing job, what is the name of those scissors you used to cut off the areolas of the cactus?

@PlantCellTechnology Жыл бұрын

That is pineapple eye remover.

@sergiohr1850 11 ай бұрын

I still curious about sterilizing the plate with alcohol and flame

@sergiohr1850 11 ай бұрын

Also I would change the plate with each catus

@Jays1khan Жыл бұрын

hi! where did you finally put the tissues? what was that oily liquid in the jar in which tissues you put?

@PlantCellTechnology Жыл бұрын

It's a sterilizing solution made of distilled water, bleach, and poly solvent 20 to clean and surface sterilize plant tissues. after explants are sterilized, you put them in a sterilized, cooled, and solidified tissue culture media and keep them in a suitable environment for their growth.

@maasjoe Жыл бұрын

Would love to see this process for various parts of cannabis

@PlantCellTechnology Жыл бұрын

Doing a Cannabis video is a bit difficult because of the legality issues. And Francisco is an expert in houseplants, carnivorous plants, and succulents.

@mariapennylane 11 ай бұрын

This is so informative and cool!! Thank you 😊

@PlantCellTechnology 11 ай бұрын

Great you found it insightful!

@gardeningonly 3 ай бұрын

Can I use same method for for succulent

@PlantCellTechnology 3 ай бұрын

Yes, you can give it a try! But, better to look for a working protocol for the species you want to grow.

@gardeningonly 3 ай бұрын

@@PlantCellTechnology thankyou

@fatmatarek8059 10 ай бұрын

What are the components of this media please?

@PlantCellTechnology 10 ай бұрын

Full MS + BAP + IBA is what we used in the media.

@CrystalDoddEnglish 4 ай бұрын

How many new cacti does each cutting grow? 1?

@PlantCellTechnology 4 ай бұрын

Yes. Each explant give rise to one that can be further multiplied to obtain many others.

@CrystalDoddEnglish 4 ай бұрын

@@PlantCellTechnologythank you! I can’t wait to try it!!

@PlantCellTechnology 4 ай бұрын

@@CrystalDoddEnglish Happy culturing!

@sergiohr1850 11 ай бұрын

Love your content, I'm working with Pitahaya and it's crazy de multiplication rate.

@PlantCellTechnology 10 ай бұрын

Thank you so much for your love and support.

@Pradeepsamanthilaka Жыл бұрын

I wonder what BAP concentration of yours is. is that 1mg/ml BAP solution?

@PollenSax Жыл бұрын

In case you don't get an answer, I'm pretty sure it's a 1g per liter hormone solution to start, then he's adding 1.5ml of that to 1 liter of grow media. 0:22

@PlantCellTechnology Жыл бұрын

Yes, that's correct!

@anamulhauqe938 2 ай бұрын

Which hormone are used for death palm tissue culture. please SMS messages me.

@PlantCellTechnology 2 ай бұрын

We aren't into date palm tissue culture. However, you can find a protocol in papers available online. Try searching "tissue culture of date palm". This should lead you to numerous relevant results.

@anamulhauqe938 2 ай бұрын

Thanks for your reply.