I am a PhD student and doing Anti cancer drug discovery and targeted drug delivery. We frequently use click chemistry to couple linker-drug with antibody. I think Click chemistry has ease the process of drug discovery by more than 50%. Super easy and quick to make a triazole ring.

DUUUDE, replicating nobel chemistry is THE GOATEST idea. Beautiful !!!

6:36 this is how you know you are a real chemist now - this is exactly the type of trick you would see from a clever peer in a research lab

Click chemistry can run in living cells. It well deserves it's Nobel Prize.

As a practicing chemist I have 2 tips that will save you much pain and time. 1) Brine washes. Any time you do an organic/aqueous extraction and your product is in the organic layer, wash it with brine before any drying step. I've made very polar substances that will go into water, but they don't go into brine. So it doesn't hurt yield (unless you spill) and it seriously cuts down the amount of drying reagent and time required to achieve a dry organic layer. 2) Buy an aquarium air pump. Spending a day doing column chromatography for such simple reactions is just pain. You can speed most columns up (not advisable with DCM/MeOH, but great for all other solvent systems) by doing it under pressure. Even a cheap fish pump is designed to maintain a steady, sometimes variable, low pressure without over-pressurizing. It will accelerate chromatography substantially for not many dollars.

one advise for column chromatography: Use sorption of crude product on small amount (5x mass of crude product) on silica gel. Disolve the product, add silica and evaporate. Than just pour it on the top of silica slope in column. This way you avoid diffusion at the begining of chromatography and problems like you had.

Nice video and showcase of click chemistry! When I run a column, I always load my sample with the tiniest amount of DCM or Acetone to avoid having it crash out of the solution, otherwise nice job!

This is by far the best video I ever watched on KZfaq, thank you very much for your explanation. Best explanation of organic chemistry work.

I use click chemistry to couple PEG to MOF drug carriers, where the azide of a organic modulator bound to the MOF reacts with a terminal alkyne on PEG. Really cool and easy stuff that you can monitor with FT-IR

Love your channel, keep up the great work 👍🏼 👏

5:30 I was a working chemist 30 years ago, before changing career completely (Catholic parish priest!). Happening on your expertly made video brought back to me the fascination of working the "classical" methods of synthesis and purification, and the sense of achievement experienced in upping yields. I'm wondering whether the low solubility of the product could be used to advantage in looking for recrystallization procedures from solvent or solvent mixtures in which the product is much less soluble than the impurities. Might this be worth a try (in place of the column) at this stage ?

Absolutely remarkable, Nobel prize well deserved on this one

One of the recipients was my Ochem professor. He was so excited about this, even before winning. The party we threw for him at UCPH was amazing. Great professor

Awesome as always!!!!!!

Nice Video Mate and very interesting!

This is one of the best videos of practical organic chemistry I probably ever saw. Click chemistry is cool.

Great! I remember all the endless column chromatography I had to perform in graduate school. So much work for so little yield. Thanks.

Great video! Very informative 👏

Wonderful work. Not a chemist, but it is nice to see a nobel creation experiment as it runs in the lab. Thank you!

Mooi man! Dit ga ik zeker in mijn les in 6V gebruiken. Top!

Very good video. I did my graduate work on organic azide chemistry. I liked you video presentation very much.

RIP vacuum pump

Cool vid! My understanding is that part of click chemistry's appeal, is that it tends to avoid the more fidgetty workup procedures like chromatography. Maybe simple recrystallization of the reaction extract residue would work?

Nifty and thanks. Heard about this approach. As a chemist that has dabbled in polymers, this sounds sort of familiar, as the stated objective often is to make molecules (usually up to about 5k or so monomers) of repeating monomers. Well done, altogether.

Thanks for sharing

I got to meet Dr. Sharpless today. He gave a lecture at my university talking about his work developing treatments for tuberculosis using Sulfur flouride exchange.

in circumstances where your crude material does not completely dissolve in your eluant, it often helps to load your material onto celite or silica, by taking a solution of your crude (in e.g. acetone), forming a suspension with the celite powder, and then removing all solvent under vacuum (with or without heating). You can then add the resulting free-flowing powdered solid directly atop the bed of sand at the top of your column

If you have an access to enough tlc plates you can try finding your own eluent for the column using other solvents. Maybe using acetone seeing it's good at dissolving the product.

Nice video ❤️

Subscribed!

Advice for column chromatography: Layer number 1: cotton wool Layer number 2: sea sand for flat surface Layer number 3: slurry of silica gel Layer number 4: pack the silica gel Layer number 5: sea sand (flat surface is required for good separation) Layer number 6: add the dilluted crude product with glass pasteur pipette (easy to clean) and use circular motion with your hands to evenly distribute the diluted crude product (gently add the diluted crude product to do not mess up the evenly distributed sea sand surface) Layer number 6 alternative: you can use celite as the carrier (flat surface is required for good separation) Layer number 7: another sea sand layer (flat surface is required for good separation) Gently add the eluent mixture to the column and have fun.

This is a really cool reaction, and I appreciate all the detail you took creating this video. I wonder how easy it is to release the tosyl group to add other substituents to the triazole ring. Also, this looks like there is a bit of Diels-Alder effect causing this "snapping into place" that's aiding the facility of this reaction. Pure chemistry doesn't get a lot of love these days from the Nobel committee.

Dude you are amazing

Well done.

Morten Meldal is my MSc professor, great teacher and very very knowledgeable in synthesis. He came up with the "hand rule" that takes advantage of the fact that our hands are chiral, let me tell you it's sooo useful! You can go from a very complex conformation and spatial disposition of the reagents and rewrite the structure while keeping all the chiral centers correct. It saves so much time and mental energy. The hand rule alone could be enough for a nobel prize 😂

I use this technique in my lab all the time. We've engineering a protein with a non canonical amino acid with an azide allowing it to be coupled to an alkyne linker

Super cool reproduction! What do you use to protect your vacuum pump from the solvent vapors? Or is it a water aspirator?

Thanks

Man that was fun

Cool video!) Remeber how I made some tetrazoles from sodium azide and nitrilles)

That's pretty cool. Could I transmit this video to a Chinese video website (bilibili) with a translation attached?

we did that reaction to produce a 5 membered ring. I remember it was a slave drive and it made me change to plasma chemistry lol

Could you show a non enzematic way to make 1 andostenediol or 2 andostendiol? Im really looming for the process of changing its locality🎉

There are so many chemicals and steps involved. How were these chemicals produced before the click reaction was invented? Also there was so much heating involved and not at room temperature.

Could this be used in the preparation of BOP or PyBOP (peptide coupling) reagent?

Jonge, wat een efficiëntie

Perfect opponent of nile red😂💕💕

I effing love this.l concept. First time

Nobel Prize chemistry. I like this.

Great video. I love chemistry but if this is "click" i would hate to perform "normal"

well would be nice if u explained how it would be used and the imprecations of it

But what can you use the created compound for? And is this the best way to make it?

How to you determine the amount of silica gel you need for the column?

Hearing the words column chromatography gives me PTSD from my silica layer cracking 5 TIMES IN A ROW. But as a Chem major I love watching these videos

what kind of vacuum pump do you have? I mean the model

The workup and purifications required for any sort of synthetic chemistry are why I'm a computational chemist.

How much amount (equivalent) of catalyst you have used?

I think butanone would be a better solvent,being more stable- amazing vid btw

I think on a technicality this is a “copper-catalysed azine-alkyne coupling” rather than a click?

Barry Sharpless has already won the Nobel Prize for the same subject, I believe. Can anyone explain the differences between the two prizes?

Do you do this in a home lab?

how about the tetrazole producing ugi 4CR?

i want to make Methamphetamine through click chemistry, please help me synthesize it!

I really regret not choosing chemistry in university, would have really liked to do this as a career. Anyway, fun to watch!

What is low radiation EMT magnetic oil?

I was hoping you would just mix two things and get the new product. I think there is another Nobel prize waiting for someone who would actually make this process much simpler.

dawg what just happened

What’s the advantage of this molecule?

Did I miss the explanation of the mechanism?

You should try loading your crude with a solvent you know is going to dissolve your crude (i.e. DCM/Et2O) with a minimal amount. Then flush your column with 100% hexanes (DCM/Et2O) gets taken away, only until then you can start flashing with your desired eluent

Can you make LSD like that?

One of the postdocs during my master's once ran a click reaction in Jack Daniels on a bet. Got something like 99% yield ^^ Didn't drink the JD after tho.

Cool

Hey, but we still need so many solvents, etc. I think then it's not a very green reaction? Or there was a reaction before for this product which was not green at all even worse than this one

Sorry but my Prof says ist was Noting new and from what i read it was the active Youse in biological Sythems what was Winning the Price Not the Reaktion ist Self

7:28 "still very simple"... 🤭

Click chem sounds like combining 2 lego pieces without glue. Sadly it dosn't work that way.

Why is acetone added to the reaction mixture? Acetone is know for reacting with itself and forming tautomers right?

I thought you have to use a reagent like TBTA to protect the Cu(I) catalyst for a complete reaction. Otherwise it would disproportionate to Cu(0) and Cu(II) which means your catalyst is not active anymore. Thumbs up if you agree😅

Why u didn't use rotavaper?

Voodoo and witchcraft (I'll stick to v=i*r and a oscope) but very cool tools.

I was going through some comments and understand one thing that lot of non chemical or less chemical background folks are here watching video. For non chemists, the name is actually missing that they thought click chemistry is like a Lego pieces snapped together, they raised concern about why to many chemicals and heating involved etc. if you know the actual process chemistry, then we understand the simplicity of this click chemistry and again in my opinion, it can be named differently.

Where is that accent from?

Instructions unclear ended up making crack in my basement.

Hype

Quantum Entanglement in Action: The Physics Behind the 2022 Nobel Prize

Gotta get this guy some air pressure for his columns!

i feel like i am watching a nilered video

I wish I could understand the significance of this but... not there yet :) Cool video though, I presume :)

The number of steps involved in this is dizzying. Where they all Really necessary to obtain this product? Given the length of time involved, and the number of chemical processes to obtain this final product, I'm missing the advantage that "click" chemistry is supposed to provide.

Why dont you use cu+1 directly, ?

Man thats why i hate column chromatography

Круто

Simple!

Is this organic chemistry?

Molecular formula of chocolate?

👍

This dude has sodium azide?! Damn, you're scaring me more than Nigel

Jee students: ye azide wagera se to mai warm up me deal karta hu

no... but you did a good 42 brother