Aurora User Training Spectral Flow Cytometry
Part 4: Titrations and Staining
0:00 Antibody titrations
2:26 Viability dye titrations
5:34 Notes about polymer dyes
8:19 Staining volume and antibody aggregates
More information on titration of viability dyes: voices.uchicago.edu/ucflow/20...
Tutorial on setting up a titration experiment within SpectroFlo: • How to Unmix Titration...
General tips on titration antibodies: • Flow Basics 2.2: Optim...
This is a comprehensive course for researchers who would like to use the Cytek Aurora. This course assumes that researchers are already familiar with conventional flow cytometry. Slides can be downloaded here: drive.google.com/file/d/1wF9-... More information and resources can be found here: voices.uchicago.edu/ucflow/sp...
View the full training course: • Cytek Aurora Training ...
Cytometry and Antibody Technology Facility
University of Chicago
I have no affiliation with Cytek Biosciences.