Really nice video. It let us know both, things common between ELISA and elispot and also what makes them different from each other. Such animations in your videos are always helpful to remember things in a better way.

Very helpful & easy to understand

U r a blessing to the biology exam aspirants 🙏... Thanks a lot sir

Very insightful, helpful and nicely done! Thank you for your work, aand I hope you are feeling appreciated.

Brilliant video, extremely helpful

Thanks! Will done!

keep on going,we support yoy

Very good animation. U forgot to mention abt stimuli. U need to add stimuli so that it will stimulate the cell to produce cytokines.

Sir if i want to analyse that which cytokines are release from the particular immune cell in respnse to a particular infectious agent which approach i can apply?

very well explained helped me a lot thank u

Very nice explanation!

very informative

Serum and plasma are acellular. You need to use PBMCs for this assay.

Very nice explanation sir ☺☺

Very Nice video, thank so much 🇩🇿

What is the advantage here in comparison to flow cytometry? In flow cytometry we do not look for "shadows" after cells, but we can actually count how many Th17 cells are there and it is much easier and faster than Elispot. Also in Elispots we do have SFUs often and it is hard to say how many cells were really there. I just don't understand why not just using flow cytometry? If someone uses PBMCs they won't know which cell really produced IFN (many do it) so Elispot will show in this case only shade of a cytokine and not the producing cells (we won't know which cell produced IFN in such case). An honest question, because I am new to Elispots and old to flow ;) I found my answer: "Flow cytometry can determine the number of cells that produce IFNγ, but not their secretory activity and the amount secreted by them. ELISPOT quantifies the frequency of IFNγ-secreting cells, but not the number of producing cells nor does it measure the amount of IFNγ secreted from cells."

Plz send some research area for elispot cytokine secreting for therapeutics and vaccine

You are gem Arpan fantastic

Helpfull👍✨️

GOD bless. Too satisfied

Sir in which diagnostic center this test is avaliable sir

Can you please clear a doubt? One cell can produce many molecules of cytokines which can subsequently bind to more than one capture antibody, and hence produce more than one spot. So why is it that one spot equals to one cell?

Love ya

Sir....could u plz clear my doubt ELISPOT is used for measuring cytokines or the secretory cells like T-cells??

Ah, Biorender :)

jiayous

Unfortunately I do not understand the English from Indian people

you are reading not explaining brother we are getting clarity on the topic 🤨