Enzyme Assay, Enzyme Activity and Specific Activity

  Рет қаралды 205,940

Andrey K

Andrey K

9 жыл бұрын

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Website link: www.aklectures.com

Пікірлер: 54
@itogisch
@itogisch 9 жыл бұрын
thanks for the clear explanation about enzyme assays. i've been trying to find an explanation without having to read 50 pages about all types of assays.
@AKLECTURES
@AKLECTURES 9 жыл бұрын
itogisch lol :-) you're welcome
@DrNem0
@DrNem0 8 жыл бұрын
Just wanted to say thank you for making this video, and explaining concepts the way you do. :) I've just started my degree in biochem, kind of been struggling in my first term because my mind is a mess! The way this video is structured however just made me focus in a way I haven't been able to for a while. I think it's the fact you started out with the real-life applications of the thing being studied, which is often not done in textbooks in the very beginning making it difficult (for me at least) to engage in the theory. The notes work well, it acts as a visual aid to where I am in the theory and I appreciate how you repeat each key point a final time - it's as if you know my mind would wander off and then by the time I become aware of this and alert you finalise the point I should've been paying attention to, but you don't overdo it as though to appear patronising. Overall, a very balanced video (in terms of different methods of learning), really taught me some things on the way I best learn too. Keep doing what you're doing!
@about_shreya
@about_shreya 3 жыл бұрын
Same here.. Just started with the course n the videos helped a lot
@ahc3363
@ahc3363 4 жыл бұрын
7:05 screen shot
@animeandstuff5377
@animeandstuff5377 3 жыл бұрын
lol nice of you but i seen ur comment after having to screenshot twice real quick lol
@dr.danishraja497
@dr.danishraja497 3 жыл бұрын
Thank u brother 😂 🥰
@ozzDeveveloperOpenForWork
@ozzDeveveloperOpenForWork 7 жыл бұрын
great explanation! I'm taking biocehm and your videos are helping a lot.
@APOLOnl
@APOLOnl 8 жыл бұрын
this is great, thanks alot 👌 a much better explenation than any of my schoolteachers. They use to over complicate things. This is a verry clear and well spoken lecture
@fatiibnmajdoub7484
@fatiibnmajdoub7484 8 жыл бұрын
Thank you so much for the clarification. Really appreciate your efforts. Best regards
@oluwapeluminexus
@oluwapeluminexus Жыл бұрын
You are such a blessing. I was excited to see that you have a video on this topic i needed clarity about because I know you would do absolute justice to it and you didn't disappoint. Thanks for all you put into this. God bless you.
@harshilsuthar5350
@harshilsuthar5350 8 жыл бұрын
thank you very much.....i am confuse between enzyme activity and specific activity after showing this all doubts goes into dustbin...good
@prudencetchouakeubetnga5718
@prudencetchouakeubetnga5718 6 жыл бұрын
thank you very much for your videos. you have help me a lot in genetic, enzymology, biochemistry and physic domain to pass my exams. Thanks a lot l really enjoy your videos
@loisvankampen3256
@loisvankampen3256 8 жыл бұрын
Thank you! I think I will be watching your video's for the entire weekend before my biochemistry exam :-)
@kibanaru221
@kibanaru221 3 жыл бұрын
That literally me rn 😂 4 years later and this hold true
@loisvankampen3256
@loisvankampen3256 3 жыл бұрын
@@kibanaru221 I passed so good luck!
@shilpasaroj730
@shilpasaroj730 4 жыл бұрын
Thank you so much for explaining this in simplified way 👏I was trying to understand this topic from books, google but ended up getting more confused.
@1730298
@1730298 8 жыл бұрын
Thanks for your best video , its really helpful just to say if you could make one more video about how to calculate other related things such as Total protein, Total activity, Yield, Specific activity and purification factor . thanks again
@sfavrgra
@sfavrgra 2 жыл бұрын
I honestly couldn't understand my lecterurs notes so by watching this video it does really helpful. Thank you so much!
@Petinency
@Petinency 6 жыл бұрын
Easiest lectures, thank you!!
@applejackzo
@applejackzo 7 жыл бұрын
You're amazing.
@saddamali666
@saddamali666 5 жыл бұрын
You're the best I wish you did a CARS series!
@Ozchuck
@Ozchuck 7 жыл бұрын
this helped. thank you.
@caterine4604
@caterine4604 5 жыл бұрын
YOU ARE MY FAV PERSON IN THE WORLD
@cybersufer_27
@cybersufer_27 Жыл бұрын
You're an excellent teacher! Thank you so much! 🤓
@jziebert1
@jziebert1 4 жыл бұрын
Excellent, well done!
@plutoniumiscool
@plutoniumiscool 4 жыл бұрын
What assay would you recomend for protease activity with microgram concentration?
@ankushsaini9977
@ankushsaini9977 4 жыл бұрын
Thanks 😊😘 for uploading video on this topic
@alyssa-abcde
@alyssa-abcde 4 жыл бұрын
Thank you very much, sir. You've helped me a lot in many, many ways.
@sakthivelb9074
@sakthivelb9074 Жыл бұрын
what is the sample used for enzyme assay
@naeemamatuoog9539
@naeemamatuoog9539 8 жыл бұрын
I would like to thank you for the clarification. can you help me . plz ? I want to know How do I test the lipase enzyme activity in the lab? (Laboratory experience for their immobilization lipase process
@TomasBruno-ww6tg
@TomasBruno-ww6tg 4 жыл бұрын
Hi. This video is great! I only have one commentary about it: the enzyme activity can actually be defined in many other ways, since it can only be measured indirectly, the units and the definition of the activity scale depends on the experiment that takes place in order to obtain such value. I think it's important to reinforce this to prevent anyone to believing this is the only possibility.
@duvetumelo7265
@duvetumelo7265 3 жыл бұрын
Quite a super tutor👍
@xuejingli1657
@xuejingli1657 3 жыл бұрын
excellent, thank you very much!
@Abbyblackblack
@Abbyblackblack 6 жыл бұрын
thank you so much !
@arunkumars6257
@arunkumars6257 Жыл бұрын
Thank you so much!
@bestcameroon9051
@bestcameroon9051 5 жыл бұрын
Hello Everyone, please I have a question regarding the calculation of relative activity of enzyme during regular intervals of Time over a long time period ( 3 weeks for example). I am working on the immobilization of cellulase enzyme for cellulose hydrolysis and I would like to estimate thé relative activity of immobilized cellulase over 1, 2 and 3 weeks. As the activity of cellulase is defined as the amount of glucose produced per minutes, its activity over a long Time ( 1, 2 or 3 weeks) will be significantly affected by the incubation Time. Then I would like to ask how Can i calculate this rekative activity taking in consideration the reaction Time ( in minutes).
@nananor3656
@nananor3656 5 жыл бұрын
What does the enzyme that has high absorabnce with lower rate of change mean?
@ashareelsrrr8089
@ashareelsrrr8089 Жыл бұрын
Helpful video
@ooiTzAmii
@ooiTzAmii 7 жыл бұрын
How does one calculate percentage recovery?
@gunslingerxxi6194
@gunslingerxxi6194 7 жыл бұрын
How to calculate the enzyme concentration?
@lorettadass3335
@lorettadass3335 3 жыл бұрын
Well explained
@josedilsondasilva8231
@josedilsondasilva8231 4 жыл бұрын
Hello. How to turn activity 1: 10000 to U / mg? Thank you very much.
@christinac1195
@christinac1195 6 жыл бұрын
thank youuu
@eruishikawa9978
@eruishikawa9978 4 жыл бұрын
How do you find out the moles of product? Lets say we have various conecentrations of an enzyme and want to figure out the no. of moles of each sample. How would you do that?
@tanvi_1840
@tanvi_1840 2 жыл бұрын
My question exactly.
@sakthivelbaskar1586
@sakthivelbaskar1586 Жыл бұрын
How to calculate the concentration
@LovelyLife365
@LovelyLife365 2 жыл бұрын
Excellently described Sir
@josefinesfilmtube
@josefinesfilmtube 2 жыл бұрын
why can you not use specific activity when high purity is required
@bhawanagahlot9540
@bhawanagahlot9540 4 жыл бұрын
I loved every vedio... But this vedio was just above my mind... I was unable to understand it
@arianalevitt3686
@arianalevitt3686 2 жыл бұрын
not all heros where capesthanks dude:)
@nandini1149
@nandini1149 Жыл бұрын
Write the words in big, because I can't understanding,it's so small wards
@adrinashafiqah1759
@adrinashafiqah1759 3 жыл бұрын
Survived online classes
@himanibhatt1170
@himanibhatt1170 2 жыл бұрын
Is there anything this channel doesn't offers ???
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