not all heroes wear cape

When you add salt to a H2O solution, you initially INCREASE the solubility of the protein as you salt in. This is due to a reduction in charge to charge interactions. As you continue to increase the salt concentration, the protein is then salted out as the salt and water compete. The entire process is a curve. Thanks for all the videos, they are excellent.

People like you deserve the heaven. Thanks for these videos. Greetings from Colombia.

Thank you sir. 6 years of pharmacy doctorate and 2 years of second master. And i have done that with the help of this channel thank you

Your videos are incredible! Thank you!

U are indeed a great teacher and great biochemist

i become a genius after watching your videos. you have helped me thru 5 semesters of upper level/detailed science courses so far !!

the video is amazing, but i wish if u explained the salting in mechanism as well cuz i looked online and i still cant find a good explanation for it.

Videos are invaluable! Our professor described the process of salting-out as "not entirely understood" hahahah. wonderful explanation.

you are so good in explanation.thanks so much

I have watched your videos since 2nd school until now in university. You sir have saved me a lot of times. Thank you so much

omg. love it. you explained perfectly! thank you!

Great video ,translates well to other languages and covers all the basics

connoisseur. .it is so enlightening to go through your lectures! Thank you heaps :)

Muchas gracias!!! Me ayudaste demasiado! Grandiosa forma de explicar :D Saludos desde Colombia!!! :)

Amazing lecture, i was so confused about this effect. Thank you.

Thank you so much for that explanation! I was slowly giving up before seeing your video.

Great explanation! You are awesome! Keep it up! Greetings from Mexico

great lecture series....please keep them coming

this is basically explained on the basis of a protein which possess hydrophilic aa on its outer surface.. that is globular proteins that is found in our blood..

thank you for contributing and sharing your knowledge to us. : ) Helped me a lot in studying by BCs in Biochemistry

Your explenations our outstanding! Thank you so much you are super helpful

Amazing lectures as usual by Ak lectures

Great lecture and a good starting point on the topic!

Genius, thank you a lot for this explanation

Great video, really helpful. Thank you

YOU ARE THE MAN! Mucho gracias

you are a wonderful teacher!

Thanks for the help! You're great at explaining!

Thank you, you do such a great job with explaining!

Great Lecture! Big Thanks from Borneo.

These videos are so clear, thank you so much!

so much thanks from Vietnam

tomorrows experiment at Biochem Lab!!!. thanks to him im not gonna look like a total dumb. Thank you so much for your help!!!

Thanks from Germany!

Great job

Awesome explanation....Keep uploading such videos

great video, very educational, but i wish i hadn't been distracted by my half-blind eyes screaming the whole time tbh i'm kinda impressed what kinda lighting did he use-

A small thank you would be less for you Sir❤ You are fantastic

From the figure, why only the hydrogen from water molecule interact with the hydrophilic part of protein? Can the oxygen interact on the hydrophilic part as well?

Pretty informative, great performance by the way!

In my book it is given that hydrophobic patches interact with water molecule please explain how is it so

Actually, most of proteins are more soluble in small concentrations of salt than in pure water (salting in). Moreover, the conventional dialysis would not separte these proteins. We should centrifuge fibrin and take out the supernatant with albumins. Then we can put fibrin into dialysis to remove salt. Nevertheless, good explanation, as always.

Thank you so much from México :)

This helped me so much thank you

Thank you so much!

awesome.. it's damn fluent and smooth..

GREAT EXPLANATION

QUESTION : will more hydrophobic amino acids precipitate first or will more hydrophilic amino acids precipitate first? I have been trying to figure this out for a while and honestly the reasoning in my head for either one kinda makes sense.

thanks again from Chile!

Thanks a lot! very clear and well explained! good stuff! cheers

U r great sir

i loved this video

Thank you very much!

For salting in (adding salt to protein) doesn't the protein solubility increase, not decrease?

Thanks from Chile ! c:

Damn. Killed it. Boss.

Thank you so much! This video was very helpfull

You are e gem

thanks

How to know which salt concentration is needed for protein? Like based on what fibrinogen requires 0.8 M salt?

I think crystallize is not the right word here, as the clusters of proteins are amorphous.

Señor, Porfavor agregue subtitulos en Español, considero muy importante su material.

thanks from brazil

If you were to add 2.4M to the solution, would it be impossible via salting out to distinguish between the two precipitates? I would imagine that if you attain the fibrogen salt concentration first at .8 M, then repeat the process with 2.4 M, you could subtract some net precipitate value from the fibrogen concentration value to attain the serum albumin value. Does this make any remote sense or am I speaking nonsense?

Helped alot! Thanks!

thank you!

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thanks from NOLA!

It was good and informative

awesome

INTERESSTING

Is there any mathematical way to determine at what salt concentration a particular protein salts out?

Proof that god is real ⬆

Thank you soooo much!

I adore you

Thanks so much. How do we separate proteins that is already dissolve in a salt. For example beta-glucosidase in citrate......... How do i get this enzyme out of this salt.

what characteristics of the proteins determine the level of salt it takes to form a precipitate

omg thank you thank youu

please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??

the video is amazing, thank you!!

great thaaaaaanks alot

thank u soooooooooooooooooo much!!!

does this mean fibrinogen is more hydrophobic than albumin? thank you !

Sir,how do we know how much concentration of salt is required for which protein?

Wow

In salting out hydrophobic interaction will decrease or increase?

🥰

What is the best method to isolate protein from beans/seeds?

6:18

Por favor en español !! Con subtítulos :(

I would appreciate it if you stopped moving around. Thanks.

Amazing lectures as usual by Ak lectures

Amazing lectures as usual by Ak lectures