Episode #14 - What Is Autofluorescence?

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Күн бұрын

Autofluorescence is the inherent fluorescence of compounds present in cells. These include compounds such as NAD(P)H, Collagen, and Tryptophan. Autofluorescence can negatively impact the sensitivity of flow cytometry measurements, especially in large cells... (Read more below.)
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Autofluorescence increases as the size of the cell increases, which can impact the background signal. It can also impact compensation, especially if the control positive and control negative have different autofluorescent signals, which will result in incorrect compensation.
Not everything about autofluorescence is bad, as some subcellular populations can be identified based on the autofluorescent signal. A perfect example of this is identification of alveolar dendritic cells as compared to alveolar macrophages.
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Пікірлер: 5

@tommerson5121 10 ай бұрын

I know autof is used in retinal examinations. My question is whether the light used to excite the cells (laser) is safe for the retina. It would almost seem that those levels of hyperintense light might be phototoxic. Any thoughts? Thanks.

@antonjets 5 жыл бұрын

Hi Tim, would you mind touching up on the comment of "reducing the sensitivity of the channel". I understand resolution (SI) quite well, but could you give a definition for sensitivity. I see a lot of flow explanations combine "resolution sensitivity" together. I know this is a bit off topic to autoflourescence but I am glad you mentioned it.

@expertcytometry6235 5 жыл бұрын

Anthony, sensitivity is defined as the ability to separate the negative signal from the positive signal. We talk about resolution sensitivity in the context of can a given detector resolve the negative and positive populations, especially in the context of a polychromatic panel.

@antonjets 5 жыл бұрын

@@expertcytometry6235 Thanks Tim, so is it safe to say that resolution and sensitivity are the same thing. I attended a training course saying that resolution is "the ability to separate the negative signal from the positive signal"

@expertcytometry6235 5 жыл бұрын

@@antonjets in this context, yes.