Designing flow cytometry panels (Intro to Flow Cytometry

Designing flow cytometry panels (Intro to Flow Cytometry - Episode 7)

Рет қаралды 8,433

Күн бұрын

Flow cytometry is a powerful technique for studying single cells. In this episode of our Introduction to Flow Cytometry series, I go over some basic principles for designing flow cytometry panels. I focus on the design of panels with fewer than 6 colours and with 6-10 colours.
Intro to Flow Cytometry Playlist: • Introduction to Flow C...
Slide deck: drive.google.com/file/d/1MPaE...

Пікірлер: 19

@suvedavigneshmaran8036 Жыл бұрын

The antigen hierarchy is such a elegant and easy to implement tip! Thanks a lot.

@crazychill6263 Жыл бұрын

Aja, I cannot thank you enough for these amazing videos. I have to teach myself flow cytometry since there´s no one in my lab who uses it on the level I need it to function and have been searching for a comprehensive, concise and well made explanation and THIS IS IT. I´m studying these videos like a bible and I´m incredibly thankful. You´re amazing and I am looking forward to any other content!!!

@ajarieger_flow Жыл бұрын

I’m glad they’ve been helpful!!

@amirmotmaen9838 Жыл бұрын

Thank you for your great videos! Even though I've been doing flow cytometry for a long time I still picked up a couple of pointers from your explanations.

@ajarieger_flow Жыл бұрын

Glad you found it helpful!

@johnnyrook6371 3 жыл бұрын

There's definitely an art to it. “Sometimes science is more art than science, Morty. Lot of people don't get that.” - Rick Sanchez

@hankramo1196 3 жыл бұрын

your videos are amazing thanks for the geat quality

@andreapaolini1268 Жыл бұрын

Great! Thanks

@homunculus777 Жыл бұрын

Excellent videos, thanks. Would love to see the advanced panel design video.

@ajarieger_flow Жыл бұрын

Noted! I am planning some new videos to release soon- I will add this to my list

@montrealnature6990 2 жыл бұрын

Thanks so much For your great explaining and video. LOVE IT SO MUCH I ll follow your channel

@djhell3054 2 жыл бұрын

Thank you so much!

@SimonCU 3 жыл бұрын

I need to setup a 20 color panel for 1 sample tube. Im going to be using Beckman Coulter's Cytoflex LX. Can someone tell me which flourochromes I should not mix together? I know FITC and AF488 should not be mixed because they have similar emission and excitation specs. I also don't like using BV510 because it is so weak and interfere with a few other flourochromes. Can someone help me?

@johnnyrook6371 3 жыл бұрын

If your facility has the option, look into Spectral Flow Cytometry. It's a lot better at unmixing similar flourochromes than traditional flow cytometry. BV510 is even brighter.

@saileshpalikhe3432 Жыл бұрын

Do we need to make compensation controls for Dapi? Assuming most cells are alive, how can I have enough events for dapi +ve cells.?

@ajarieger_flow Жыл бұрын

Yes you should still for any viability dye. For these controls you will want to use cells that are somewhat dead... heat shock, repeated freeze/thaw, or addition of chemicals that induce cell death are all common methods here

@arora1991 Жыл бұрын

Can I use BB700 and BV711 in the same panel? The filters are the same (710/50) but in different lasers.

@ajarieger_flow Жыл бұрын

It will ultimately depend on the optical configuration of your instrument- but if all lasers are spatially separated, it should work. I would however recommend that you don’t match them with markers on the same cell type

@mohamedmahmoudsaad6499 2 жыл бұрын

Thanks for the informative series, I really appreciate it, but could you pls avoid using the background music in the future as it makes the voice less clear. Thnx again.