How ions produce an electrical charge on a semi-permeable membrane by the a Gibbs-Donnan Equilibrium
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@llkeeley11 жыл бұрын
Thank you for letting me know that, even in retirement, I am still able to help classroom education. LK
@georgelsoare81572 жыл бұрын
You are still doing so :) congratulations! Thank you so much for your work.
@emiellebloemendal196011 жыл бұрын
This tutorial was used for a 3rd year physiology lecture, at the University of Pretoria, thanks for making studying easier :). This tutorial is pretty cool.
@llkeeley11 жыл бұрын
Thank you, I am happy you found it of value.
@sounakrakshit73122 жыл бұрын
Thank you so much..even after 10 yr it's continuously helping student across the globe..
@ahmadpavadiya4 жыл бұрын
4:22 is deciding factor for gibs donnan equilibrium Thank you for clearing my doubt ❤️❤️🔥👍
@flageyme11 жыл бұрын
This was really well done!! thnx a lot from a student in belgium ;)
@michaelhartman165610 жыл бұрын
Thank you so much for this video, nicely done, very graspable.
@llkeeley10 жыл бұрын
You are most welcome. In my opinion, this is one of the most difficult concepts in physiology to get one's mind around, and I am happy if I have been able to help you understand it.
@dojoofficial7 жыл бұрын
Thank you so much Sir. Very clear explanation.
@llkeeley Жыл бұрын
If I understand your question correctly, the charge difference occurs across the membrane not on one side. On either side the numbers of =/- charges are equal but, between the two sides one side has more + charges vs - charges so the charge occurs between the two sides.
@Klatregutten10 жыл бұрын
Sweet fast review of the Donnan Equilibrium. Makes understanding the phenomenon much easier!
@llkeeley10 жыл бұрын
Thank you. Happy that you found it helpful.
@llkeeley11 жыл бұрын
Thank you for the nice compliment. I use Adobe Flash.
@sweetpicklez8 жыл бұрын
Thank you this was very clear!
@fattons9210 жыл бұрын
really really really really thank u! i was loosing my mind trying to undertand it!
@ahmadpavadiya4 жыл бұрын
Me too bro
@haroonrashid50855 жыл бұрын
Crystal clear explanations . Thank you
@llkeeley5 жыл бұрын
My pleasure to be of assistance
@maryamjaved15714 жыл бұрын
Thank u so much for this wonderful video.It made me understand the entire topic so easily.
@llkeeley4 жыл бұрын
You are welcome. Happy that I could help you understand this difficult topic,
@rinafadlina39058 жыл бұрын
Thank you! I am finally able to understand this :D
@Sarah-gw7bj4 жыл бұрын
really helpful, thanks a lot!
@BAYK864411 жыл бұрын
Thanks a lot for this very helpful and inspiring video!! I have been brooding over one detail: For calculation of Gibbs-Donnan effect on the resting membrane potential (as opposed to the action of the sodium pump) it would be necessary to know the concentration (number of charges?) of the intracellular impermeant anions in an actual (mammalian) cell (e.g. a neuron). Do you have any information about this concentration? I was unable to find usable numbers. Thanks again! CR.
@llkeeley11 жыл бұрын
Good point. This is strictly a phenomenon of solutions with any semi-permeable membrane with common, permeable ions on each side and an impermeable ion on one side. It would happen with a synthetic membrane or a biological one. Since this is a physiology education website, I relate it to biology.
@luxemshah88016 жыл бұрын
thanks!want to see more!
@parampreetkaur58294 жыл бұрын
Really good. Thanks dear
@aklilukefyalew9992 жыл бұрын
Really helpful, Thanks
@anonymea43264 жыл бұрын
Even after so many years your video is so helpful! thanks!!
@llkeeley4 жыл бұрын
You are welcome
@jainamshah481910 жыл бұрын
Excellent very nicely explained thank you
@llkeeley10 жыл бұрын
You are most welcome. Thank you for the comment.
@noujumnoor7157 жыл бұрын
thank you so much for this video... 😊
@llkeeley7 жыл бұрын
You are welcome LK
@taeilshighnotesinchain56214 жыл бұрын
Very clearly explained. thank you very much.
@llkeeley4 жыл бұрын
Thank you. Always happy to know it was useful.
@samuelmcgill91689 жыл бұрын
K+ and Cl- are should be opposite in relative size
@Mansoorali-zq1rp4 жыл бұрын
Thanksssa for this
@omkarchavan59407 ай бұрын
How did you calculate ion concentrations at equilibrium?
@lingyuzhang71902 жыл бұрын
Hi, just a quick question: why [K]inside*[Cl]inside = [K]outside*[Cl]outside at the equilibrium? thanks!
@llkeeley11 жыл бұрын
There are many impermeable anionic species (Pr) inside the cell, but it is not necessary to know their content, since they have no equilibrium. They set the conditions for the movement of the permeant ions (i.e. negative inside). The Nernst Eqn is based only on the equilibrium of the permeable ions therefore, the charge is Ko vs. Ki; [Na]o vs [Na]i; and [Cl]i vs [Cl]o. [Pr -] = 0 outside, so they do not contribute to the final equilibrium or the calculation which is entirely the permeable ions
@kellieskawewe75055 жыл бұрын
U are the man sir
@ayaboussaada72094 жыл бұрын
thank you
@DarrellBarnes7 жыл бұрын
Does the slow movement of K+ back and forth across the membrane explain why my blood pressure response to eating too much sodium seems to be delayed a day or two after the incident? It seems like I get both immediate and delayed effects of Na+/K+ consumption reflected in my blood pressure. This video was assigned by Dr. Turnbull at Clemson for a Zoology class! Interesting!
@llkeeley7 жыл бұрын
I do not think so. It may be slow in my video, but I am illustrating events not how fast they occur in reality. Your body is constantly adjusting your blood pressure based on its regulatory signals and needs. If these are not functioning properly, I recommend you discuss this with your doctor and follow his advice. Tell Dr. Turnbull thank you for me.
@clarashakivsoyris13367 жыл бұрын
Hi,at equilibrum - does the osmalarity is equal in each side ?
@llkeeley7 жыл бұрын
No. If one is dealing with a semipermeable, non-living membrane the molarity of solutes is higher on the side with the impermeable anions. So water would flow by osmosis from the side with only permeable ions such as Na, K, and Cl into the side with the non-permeable ion. In living cells, the membrane is more complicated with regard to differential ion permeablities. Cell membranes are very permeable to K+, about 50% less permeable to Cl- and only about 10% as permeable for Na+. Also, there are large cationic proteins in the extracellular fluids of animals, so the osmotic effects are offset. Only in extracellular fluids of distinctly higher or lower osmolarities would there be a problem or, possibly, if the cells were injured and their membrane permeabilities damaged.
@MysticMD9 жыл бұрын
thanks so much :)
@llkeeley9 жыл бұрын
+Zi Gyllenhaal You are welcome. LK
@emanuelferraro2267 Жыл бұрын
How can one side have a negative charge if the numer of cations and anions in it it´s the same?
@hitokirib22296 жыл бұрын
Why does the number of penetrable ions (which is greater on the outside) make the inside of the cell more negative than the outside if both K+ and total negative charges are more on the inside than on the outside at equilibrium?
@subhrabhattacharjee25385 жыл бұрын
Because of concentration gradiant
@anushaanthony33157 жыл бұрын
thank u
@llkeeley7 жыл бұрын
You're welcome
@RomeroPiano5 жыл бұрын
Does this apply for capillary phenomen about oncotic pressure?. Because the compartiment there are Intravascular and intersticial and the negative protein is Albumin.
@llkeeley5 жыл бұрын
Yes, it would. Osmotic pressure is for water movement and if there are unequal amounts of solute in solution, there would be a lower water concentration in the more concentrated solution so water would flow into the more concentrated solution. But the picture is complicated in cells in that you have differential permeabililties of membranes to ions, and also active and passive ion pumps and channels in the membrane. This explanation is a relatively simple system where everything is based on differential membrane permeability and 3 ions of which one is impermeable. As explained in the last scene where I talk about the cellular system, be aware that the GB-equilibrium only occurs along the cell membrane whereas the osmotic effects involve the entire cell and its entire solute concentration relative to the extracellular milieu. I am not sure that is a very satisfactory answer, but the physical electrochemical properties still always apply but are more complicated in biological systems. That is why we use 0.9% saline solutions in physiological systems: to maintain both isotonic conditions and as a source of sodium and chloride Hope that helps?.
@RomeroPiano5 жыл бұрын
@@llkeeley Yeah, I got it. I was just wondering...if something similar occurs when we talk about insterticial and intravascular space I would assume that there's a membrane electric potencial around every capillar. Look this image > www.mailxmail.com/curso-equilibrio-hidroelectrolitico-acidobasico/distribucion-electrolitos-organismo Na+ are gonna start to move to insterticial space creating a potencial 'cause negative-charged protein can't get through the endotelium.
@clarknielson41815 жыл бұрын
God bless you.
@llkeeley5 жыл бұрын
Thank you. I need all the help I can get.
@manigandanc37525 жыл бұрын
Larry Keeley thank you
@Sara334611 жыл бұрын
Biological membrane or a sheet of plastic may I ask?
@kpshekhawat10 жыл бұрын
i am biochemistry student. the topic is nicely explaind
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@kpshekhawat10 жыл бұрын
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@llkeeley10 жыл бұрын
Thank you
@TuNguyen-yv7lv3 жыл бұрын
could you answer my question, please. why [Na]1[Cl]1=[Na]2[Cl]2. if i [Na]1x[Cl]1=(x+y)y=[Na]2x[Cl]2=(x-y)^2 xy+y^2=x^2-2xy+y^2 x=0 or x=3y so it isn't true in every situation. could you explain why our bodies follow this rule. if it is not correct so the example at 6:49 is not objective, it just reflect a special situation
@llkeeley3 жыл бұрын
I apologize but I have no idea what your formulas are with all the double arrows, or how they relate to what I show in the video. The at equilibrium
@user-cz6gm4dd2h9 ай бұрын
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@constanzagimenez59914 жыл бұрын
do you can to put the video in spanish?????????????????? pleassssseeee
@llkeeley4 жыл бұрын
I would like to, but my translator only was interested in doing the neurotoxic insecticides. I am considering to see if I can do it in Google translate as either closed caption (written Spanish) or maybe somehow record the computer voice translation using Google translate. But this likely will not be soon. but thank you for the request.