How to calculate Km and Vmax values - Lineweaver Burk plot in Excel

Рет қаралды 162,412

3 жыл бұрын

This video explains about How to calculate Km and Vmax values - Lineweaver Burk plot in Excel.
Km and Vmax value calculation in excel from the enzyme kinetic data. Michealis menton constant (Km) can be accurately calculated from the lineweaver burk plot in excel.
Enzyme Kinetics: Lineweaver Burk Plot Explanation:
• Enzyme Kinetics : Line...
Related Videos:
---------------------------------------------------------------------------------------------------------------
Plotting Bacterial Growth Curve: • Plotting Bacterial Gro...
Colony Forming Units: • CFU: Colony Forming Un...
Serial Dilution Methods: • Serial Dilution Method...
Copy Number Calculation for qPCR: • Copy Number Calculatio...
How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: • How to analyze Primer ...
How to generate qPCR standard curve in excel and calculate PCR efficiency: • How to generate qPCR s...
Real Time qPCR optimization, Calculating PCR Efficiency: • Real Time qPCR optimi...
Taqman Assay Vs SYBR Green Assay: • Real Time PCR Assays- ...
Resolving poor PCR efficiency: • Resolving Poor PCR ass...
Calculating molecular weight of Unknown protein • How to calculate molec...
Designing qPCR Primers
• Primer designing for r...
Oligonucleotide Preparation & Purification • Oligonucleotide Prepar...
How to check Oligo Concentration
• How to check the Oligo...

Пікірлер: 93

@manny_k2988 3 жыл бұрын

Absolute lifesaver. Super straight forward video, thank god I didn't have to sit through a 20min video explaining 20 billion different derivations of the the MM equation

@tamaradifc7566 Жыл бұрын

Thank you. We pay thousands in the college and my bio teacher does not even bother to explain anything properly. Thank you 🙏

@mlawliet6151 7 ай бұрын

You’re a lifesaver! I didn’t get how to do it _at all_ until I watched the video. Your tutorials are a gem 💎

@amandaouk8962 Жыл бұрын

i have been finding an example of how to properly do it. Very straightforward and exactly what i was looking for!

@allexis5803 2 жыл бұрын

Best video of this topic I have found, I really couldn't understand this topic but you made it so much simpler to understand for my report! Thankyou, subscribed!!

@abbiepalmer4547 Жыл бұрын

lifesaving video!! thank you so much

@user-bu6tk1mq7j 8 ай бұрын

absolutely brilliant video, thank you so much for putting this up! It was so easy to understand and literally saved me hours of work!

@blabiblabiblabi 3 жыл бұрын

Thank you so much. I did an experiment to determine Km. Now i know how do calculate it

@alphazenturbo Жыл бұрын

Very straight forward. I needed to a L-B for Pchem and this was extremely helpful for making the graph in Excel.

@BioResource Жыл бұрын

Thank you. Consider subscribing.

@beehyve Жыл бұрын

Explained it so well thank you so much!!

@mr.legend368 2 жыл бұрын

Thanks brother.... I've seen lots of videos about problem but only ur video clear doubt problem

@dentedtester36 2 жыл бұрын

Thanks so much, makes much more sense now!

@daniellerochagiro3476 2 жыл бұрын

Thank you so much!!! This video just save my semester. GOD bless you!

@BioResource 2 жыл бұрын

Glad it was helpful. Consider subscribing for more such videos. Thank you

@rosemaryjude26 Жыл бұрын

Thank you for this video. It was straight forward and detailed. You have earned a subscriber😃

@isaiasv9925 2 жыл бұрын

man thank you soooooooo much this was so helpful needed to do this for a final exam and it explained what I needed to do so well thank you sir, may you have great days ahead of you

@BioResource 2 жыл бұрын

Glad it was helpful. Consider subscribing for more such videos. Thank you

@JJH2004 2 жыл бұрын

THIS IS EXACTLY WHAT I NEEDED, THANK YOU SO MUCH!!!!!

@BioResource Жыл бұрын

Thank you. Consider subscribing.

@montserrathermosillo780 2 жыл бұрын

Thank you so much, your video saved 4 biology students today. Greetings from Mexico :)

@BioResource 2 жыл бұрын

Glad it was helpful. Consider subscribing for more such videos. Thank you

@rawyld 5 ай бұрын

Thank you so very much.

@mojzarea Ай бұрын

Oh, Thank you so much, you life saver man

@hewyin6130 3 жыл бұрын

thank you!!! this saved me

@michellemandelstam8633 3 жыл бұрын

Thank you so much!!!

@fizakhalid8537 6 ай бұрын

thank you! this was so useful😀

@Not-Nur 2 жыл бұрын

perfect video, thank you

@LucasJensenOfficial 3 жыл бұрын

Very well explained video. Thank you!

@BioResource 3 жыл бұрын

Thank you.

@kelvineivo 3 жыл бұрын

Thank youuuuuuuu. You got no idea how much this video helped me .

@BioResource 3 жыл бұрын

Glad it helped!

@mariebrassart9868 3 жыл бұрын

Very clear and helpful, thank you ! 😊😊😊

@BioResource 3 жыл бұрын

Glad it was helpful!

@axediepied6993 2 жыл бұрын

Amazing work, I understood perfectly.

@BioResource 2 жыл бұрын

Great!. Consider subscribing for more such videos. Thank you

@daniel98carvalho 3 жыл бұрын

Great video! Thank you very much!

@BioResource 3 жыл бұрын

Glad you liked it!

@anaa7595 2 жыл бұрын

THank you for making this video! IT was so helpful!!

@BioResource 2 жыл бұрын

Glad it was helpful. Consider subscribing for more such videos. Thank you

@GrayGhost_CV-6 7 ай бұрын

Thx sir, this so helpful for me

@rspeks9461 3 ай бұрын

OMG thank you so much!!!

@rspeks9461 3 ай бұрын

Got me through my chemistry assignment!

@DeStIiNix99 Жыл бұрын

Thank u sir 🔥

@katsuno5916 Жыл бұрын

what a life saver, explained it very well

@BioResource Жыл бұрын

Thank you. Consider subscribing.

@gud_soup6991 2 жыл бұрын

Thank you brother, much appreciated 🙏

@BioResource Жыл бұрын

Thank you. Consider subscribing.

@jl2584 Жыл бұрын

really appreciate your work!

@BioResource Жыл бұрын

Thank you. Consider subscribing 😊

@nikitarashinkar1897 4 ай бұрын

Thank you 😊

@wei2183 Жыл бұрын

very useful ,thanks a lot 非常有用謝謝你

@katecherieandreallo8874 Жыл бұрын

So helpful ! Thankyou!!

@BioResource Жыл бұрын

Thank you. Consider subscribing 😊

@khadijahahmed1970 Жыл бұрын

Thanks mate

@aishwaryaramkumar1221 2 жыл бұрын

Soo helpful honestly a lifesaver for my last minute lab report

@BioResource 2 жыл бұрын

Glad it was helpful. Consider subscribing for more such videos. Thank you

@concurseiroentropy4483 3 жыл бұрын

Very nice!

@samanthac323 3 жыл бұрын

This was so helpful. Thank you!

@BioResource 3 жыл бұрын

Glad it was helpful.

@captainlux409 Жыл бұрын

Best explained video, thank you sir!

@BioResource Жыл бұрын

Thank you. Consider subscribing.

@manonmclellan7304 2 жыл бұрын

This video saved my biochemistry report thankyou!!!!

@BioResource Жыл бұрын

Thank you. Consider subscribing.

@Koeliaify 2 жыл бұрын

The explanation is really very helpful

@BioResource Жыл бұрын

Thank you. Consider subscribing.

@maxw5900 3 жыл бұрын

Thank you, Good Sir!

@BioResource 3 жыл бұрын

Welcome!

@user-nt2jf7oc2i 3 жыл бұрын

Saved my life man 🙌🏼🌷🌷

@BioResource 3 жыл бұрын

Glad it was helpful.

@aqsaqureshi3833 3 жыл бұрын

Mine too

@berkayadem7197 Жыл бұрын

thank you very much

@BioResource Жыл бұрын

Thanks. Consider subscribing 😊

@zaidmaniar2899 Жыл бұрын

how did you get the initial velocities/product formed ?

@brianbridges8124 3 жыл бұрын

hello, why do you bring the line to - 0.5, im wondering how u know what vmax is?

@songulates7833 3 жыл бұрын

Thank you so much but i didn't understand , why is backward=0.5 periods for forecast?

@kaoutar8656 3 жыл бұрын

you are amazing

@danielhuelgas5355 3 жыл бұрын

Thanks a lot!

@BioResource 3 жыл бұрын

Welcome!

@justflow7769 Жыл бұрын

thank you so so so so much !🥰

@BioResource Жыл бұрын

Thank you. Consider subscribing.

@zaysiz 3 жыл бұрын

Sometimes, I get Lineweaver-Burk equation as y=ax-b. While 1/Km is possitive, 1/Vmax is negative. Is it problem? Why is it negative?

@malleshgampa1956 3 жыл бұрын

Substrate concentration we have to take in mM?

@rakshamukhia7970 Жыл бұрын

Sir, I do not understand but Iam gettingthe equation plotted in the graph with a ngative sign. where iam I flawed? y=10.192x-0.3494

@foreverninteen8580 3 жыл бұрын

What is the unit of substrate concentration [S] here?

@usmanabdulkareem7699 3 жыл бұрын

Nice

@misbahhusolli 2 жыл бұрын

I found difficult when use this plot for bacteria fermentation kinetic, where the curve of 1/s and 1/u are not the same as the literature. the line of the curve is downward, Y = -ax + b, but the R square = 0,9 kind of. what if the result of this plot is downward curve, is it still can be use as a data to calculate Km?

@ximena5063 2 жыл бұрын