This video lecture explains
1. What is the definition of immunohistochemistry (IHC)?
2. What is the principle of immunohistochemistry?
3. What are the different steps involved in the immunohistochemistry procedure?
4. How sample is prepared in immunohistochemical analysis?
5. What is antigen retrieval and why is it important ?
6. How and why blocking is performed in immunohistochemistry?
7. How target antigen is detected?
8. What are the differences between fluorescence-based detection method and colorimetric based detection method ?
9. How the samples are visualized in immunohistochemistry?
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