#sterilization_and_disinfection #sterilization_and_disinfection_in_hindi #method_of_sterilization #hot_air_oven #autoclave #physical_method_of_sterilization #chemical_method_of_sterilization #microbiology #dr_najeeb_lectures
Introduction of Sterilization
Introduction of disinfection
Physical method of sterilization
Chemical method of sterilization
Antiseptic
Fumigation
Digantamination process
Autoclaving method
What is hot air own
Use of hot air oven.
Use of autoclaving
Radiation method of sterilization
X-ray radiation
Covering questions:-
What are three methods of sterilization ? What are the types of sterilization ?
What are the 4 methods of sterilization ? What do you mean by sterilization ?
What is the principle of sterilization ? Does sterilization kill viruses ?
Is sterilization the same as disinfection ? Why sterilization is required ?
Does 70 alcohol kill viruses ?
What is gas sterilization used for ?
What is chemical method of sterilization ? What are autoclaves used for ?
What is the best method of sterilization ? What is sterilization of water ?
What is sterilization and its methods ? What is the process of sterilization ?
What are sterilization procedures ?
What is sterilization and disinfection ? How long does cold sterilization take ? How do you sterilize a paperclip ?
How do sterilization pouches work ?
Do sterilization pouches expire ?
Can you reuse sterilization pouches ?
Do sterilization pouches expire ?
Can you reuse sterilization pouches ? How do autoclaves sterilize ?
Why do Autoclaves use pressure ?
What are the steps to autoclave ?
Why are autoclaves used ?
What are autoclaves used for ?
What materials can be autoclaved ?
What Cannot autoclaved ?
Can you boil needles ?
What is sterilization process ?
Is female sterilization painful ?
What is the principle of sterilization ? What is the purpose of sterilization ?
What is the safest disinfectant ?
Sterilization and disinfection are the basic components of hospital infection control activities. Every day, a number of hospitals are performing various surgical procedures. Even more number of invasive procedures are being performed in different health care facilities. The medical device or the surgical instrument that comes in contact with the sterile tissue or the mucus membrane of the patient during the various processes is associated with increased risk of introduction of pathogens into the patient's body. Moreover, there is chance of transmission of infection from patient to patient; from patient or to health care personnel, and vice versa; or from the environment to the patient through the improper sterilized or disinfected devices. Hence, medical personnel, laboratory people and the health care providers should have better knowledge regarding these techniques to prevent the spread of these pathogens.
Chemical sterilant: These are chemicals used for a longer duration (3-12 h) to destroy all forms of microbes, e.g., peracetic acid (PAA) (0.2%), glutaraldehyde (≥2.4%), ortho-phthalaldehyde (OPA) (0.55%), and hydrogen peroxide (7.5%).
Disinfection: Disinfection is defined as a process of complete elimination of vegetative forms of microorganisms except the bacterial spores from inanimate objects. Technically, there is reduction of ≥103 log CFU of microorganisms by this method without spores.
High-level disinfectant (HLD): Used for shorter duration and able to kill 106 log microorganisms except spores, e.g., glutaraldehyde (≥2.0%), OPA (0.55%), hydrogen peroxide (7.5%), hypochlorite (650-675 ppm), and hypochlorous acid (400-450 ppm).
Intermediate level disinfectant (ILD): These disinfectants act against Mycobacterium tuberculosis and are mainly used for noncritical items contaminated with blood/body fluids.
Low-level disinfectant (LLD): LLDs are used to remove the vegetative form of bacteria, few fungi, and some enveloped viruses from the noncritical items, e.g., 3% hydrogen peroxide, quaternary ammonium compound, diluted glutaraldehyde, phenolics, etc.
Decontamination and cleaning: Decontamination is the process of removal of pathogenic microorganisms from objects so that they are safe to handle. Cleaning is defined as removal of visible soil (e.g., organic and inorganic materials) from the surfaces and objects. Technically, it achieves minimum reduction of ≥1 log CFU of microorganisms.
Antisepsis: Antisepsis is a process of removal of germs from the skin. When it is related to the patient’s skin, it means disinfection of living tissue or skin. When it is related to the health care worker, it means reduction or removal of transient microbe from the skin.
Germicide: It is the agent that destroys germs. It includes both antiseptics and disinfectants. The type of microorganism is identified from the prefix (e.g., virucide, fungicide, bactericide, sporicide, and tuberculocide).
Sterilization, Disinfection, and Cleaning in the Health Care Facilities
Sterilization, disinfection, and cleaning