Microbiome Informatics: OTUs vs. ASVs | Zymo Research

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Zymo Research

Zymo Research

Күн бұрын

In this video, we review the basic concepts of OTU and ASV-based methods of targeted microbiome sequencing resolution. Topics will include a basic introduction to targeted microbiome sequencing, the methods and logic behind both OTU and ASV generation, and the advantages and disadvantages of each method for 16S and other targeted sequencing studies.
Read more about OTUs vs ASVs, including the advantages and disadvantages of each when dealing with difficult sample types here:
www.zymoresearch.com/blogs/bl...
To find out more about how we can assist in your microbiome studies, please check out: www.zymoresearch.com/pages/mi... and www.zymoresearch.com/pages/zy...
Be sure to subscribe to our channel for more bioinformatics and microbiomics content!

Пікірлер: 12
@zunjuu
@zunjuu 4 жыл бұрын
Thanks, thats a brilliant video. The last point made me smile. How many times have we all read "OTU grouping" autocorrected to "OUT grouping".
@hpremathilake
@hpremathilake 4 жыл бұрын
Like the concluding remarks :D
@adeelmanzoor9801
@adeelmanzoor9801 2 жыл бұрын
I think the instructor should slow down as speedy talk make understanding such technical topics more difficult.
@ktburger659
@ktburger659 Жыл бұрын
Great video, thanks so much
@soniavargas7947
@soniavargas7947 2 ай бұрын
this was an excellent video, thank you so much! The autocorrect made me laugh, as this has victimized me in the past.
@loxix100loxix100
@loxix100loxix100 Жыл бұрын
how do we know if the sequence is or isn’t conserved?
@Crazy09starkillor
@Crazy09starkillor 3 жыл бұрын
If i were to use ASV, do I still need to run DADA2 or amplicon denoise before hand?
@couchpatata6835
@couchpatata6835 7 ай бұрын
When you run your sequences using DADA2 (I used the DADA2 plugin in QIIME2), your output are ASVs.
@kiawendy745
@kiawendy745 11 ай бұрын
fantastic video, thanksss
@ZymoResearch
@ZymoResearch 8 ай бұрын
Glad you liked it!
@pankajchand6761
@pankajchand6761 2 жыл бұрын
Why does the speaker need to speak so fast when explaining a technical topic?
@edodanilyan2426
@edodanilyan2426 2 жыл бұрын
Well, you can slow it down, there's a feature to do that in KZfaq
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