Sorry, I can't give a 'super thanks', but I would like to express my sincere thanks for your videos. I am able to understand a lot about biotechnology and then passed my exam. I'm a vet student from Indonesia. 🙏
@animatedbiologywitharpan Жыл бұрын
Please share my channel link with your friends and help me to reach big audiance.
@alejandropikoulasplata5524 Жыл бұрын
Thank you so much for these very useful short tips!
@animatedbiologywitharpan Жыл бұрын
Thanks for your support. Please feel free to suggest more video topics in comments.
@jagjyotkaur723 жыл бұрын
Very well explained thanks !! The animations are really good
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience.
@jagjyotkaur723 жыл бұрын
@@animatedbiologywitharpan yes I did share it with my friends it is really helpful
@IronMan-dj1uw Жыл бұрын
Hello Bro You have not included the further steps Like After making the hybrid rna-dna .The hybrid's rna is partially digest by Rnase H ,then DNA polymerase I forms a complimentary strand and nick is sealed by DNA ligase and Thus forms a cDNA
@animatedbiologywitharpan Жыл бұрын
Yes these are nitty grity details. It was a overview hence did not get into details
@davidace72 жыл бұрын
very good, detailed and concise explaination, thank you sir!
@animatedbiologywitharpan2 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@divyasvlog44102 жыл бұрын
@@animatedbiologywitharpan sure sir ... lov your explanation
@ikennafranklinokoro4252 Жыл бұрын
Great video, comparison of RT-PCR and PCR was a benefit
@animatedbiologywitharpan Жыл бұрын
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
@priyankachauhan49765 ай бұрын
I can't thank u enough for this... just keep going 🙏🙏🙏
@animatedbiologywitharpan5 ай бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@hidayat.6098 ай бұрын
awesome
@animatedbiologywitharpan8 ай бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@sangitamandal6146 Жыл бұрын
It was very helpful ❤thank you sir😊
@animatedbiologywitharpan Жыл бұрын
Please share my channel link with your friends and help me to reach big audiance
@geekymedico.80993 жыл бұрын
Amazing explanation ... thank u sir...🙏🙏🙏🤗🤗🤗🤗🤗🤗
@mariafernandalealtamez98443 жыл бұрын
Very good info! Do you have the references??
@SuperLinhi2 жыл бұрын
I have a question: when I have transcribed the mRNA into cDNA, do I perform a normal PCR with the cDNA? Can someone pls tell me if i'm right or wrong ? PCR steps 1) denaturation of the template DNA 2) use primer and poymerase 3) amplification RT-PCR: 1) isolation of RNA 2) transcribe with reverse primer and enzyme reverse transcriptase into cDNA 3) follow PCR steps
@user-so7ng7bf8x Жыл бұрын
Thanks, good content, should give you thumb up.
@animatedbiologywitharpan Жыл бұрын
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
@dhamotharan67453 жыл бұрын
Sir can u explain about multiplex pcr
@animatedbiologywitharpan3 жыл бұрын
Sure it would be uploaded soon
@yokoshiko94272 жыл бұрын
Great help on exam eve😇🤩
@animatedbiologywitharpan2 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@nilufertek31772 жыл бұрын
you saved my todays prentation sir, thank you very much!
@animatedbiologywitharpan2 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@alperentur15433 жыл бұрын
very nice channel thanks from turkey arpan
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience. By the way I love 💘❤ Turkey and the amazing food. I still remember my visit to Istanbul.
@animatedbiologywitharpan3 жыл бұрын
I just love baklava and kebabs
@anusreesaha4624 Жыл бұрын
Please upload a video on allele specific PCR.
@animatedbiologywitharpan Жыл бұрын
Thanks for suggesting
@n0skies Жыл бұрын
thank you
@animatedbiologywitharpan Жыл бұрын
Please share my channel link with your friends and help me to reach big audiance
@umapriscilla6243 Жыл бұрын
Thank u sir
@animatedbiologywitharpan Жыл бұрын
Please share my channel link with your friends and help me to reach big audiance
@sanajiya44629 ай бұрын
Yawrrr such a super duper lecture thank you so much🥹✌️
Please share my channel link with your friends and help me to reach big audience
@Yousha9993 жыл бұрын
Hi thank You for the well explained video can you explain microRNAs
@animatedbiologywitharpan3 жыл бұрын
Ya there would be series on all the regulatory RNAs soon
@animatedbiologywitharpan3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@shubhamrana419993 жыл бұрын
sir is rt pcr is same technique used in covid rest or it is different .
@animatedbiologywitharpan3 жыл бұрын
That RT pcr is real time PCR also qPCR . One step of that is reverse transcription which makes cDNA from RNA . After that qPCR is done from the cDNA.
@__jm__67622 жыл бұрын
Dont we use a specific primer in RT pcr with those 2 primers?
@animatedbiologywitharpan2 жыл бұрын
Generally no. In Reverse transcriptase PCR all mRNA are converted to cDNA . Ideally there should not be any bias. During qRT PCR( quantitative real time PCR ) we use specific primers.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@animatedbiologywitharpan7 ай бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@jennymacparry18552 жыл бұрын
What if we don't use hexamer primers???
@animatedbiologywitharpan2 жыл бұрын
Alternatively you can use oligo dt
@sabinabegum419 Жыл бұрын
Inverse pcr k upar ek banayega plz
@animatedbiologywitharpan Жыл бұрын
kzfaq.info/get/bejne/rNp4qrKIsra4fGQ.html
@sanjeevyadav19762 ай бұрын
Sir is this technique used for hiv detection
@animatedbiologywitharpan2 ай бұрын
can be used
@prachimate2238 Жыл бұрын
can u plz explain tma and nasba
@animatedbiologywitharpan Жыл бұрын
Can you tell me the full forms
@Xahidd____2 ай бұрын
Can anybody tell me if reverse transcriptase posses 5'-3' exonuclease activity then why aren't Hexamers degraded instead they are incorporated 😢😢
@ritikyadav30143 жыл бұрын
Hello sir, i want to go for research but i don't know how to find research institute for my topic of research can u help me that how can i search institute.
@myeverydaylife202 жыл бұрын
hello dear. The RT-PCR consists in 1 cicle only ??
@animatedbiologywitharpan2 жыл бұрын
For reverse transcription protocol yes
@animatedbiologywitharpan2 жыл бұрын
For q RT PCR ( quantitative real time PCR ) se the following video kzfaq.info/get/bejne/ldZ9dtqDqreWYn0.html
@myeverydaylife202 жыл бұрын
@@animatedbiologywitharpan Thank u so!!!!
@thisslightlysweetlife34022 жыл бұрын
Where does the reverse transcriptase itself come from?
@animatedbiologywitharpan2 жыл бұрын
You provide it in the reaction mixture, reverse transcrtase for this purpose is syntheticly made.
@thisslightlysweetlife34022 жыл бұрын
@@animatedbiologywitharpan In vivo I mean. Does it exist within our bodies or does the viral RNA code for it as well.
@Apoorva41022 жыл бұрын
@@thisslightlysweetlife3402 it's isolated from AMV virus :)
Please refer the references i need to write my notes thanks in advance
@animatedbiologywitharpan6 ай бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@Jesusandbible2 жыл бұрын
if you claim to be knowledgeable ...... is this a real quotation or not? “Quantitative PCR is an oxymoron.” Kary Mullis, inventor of PCR. As it it is then almost all the stats we have about Covid are bunk.