Salting In and Salting Out of proteins

Рет қаралды 120,916

8 жыл бұрын

Salting in and salting out - This lecture explains about the salting in and salting out of proteins.
Salting in and salting out are two different types of method for the extraction and purification of proteins.
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Thank you for watching the lecture video on Salting In and Salting Out of proteins.

Пікірлер: 213

@ehatipo4598 3 жыл бұрын

You're probably the best Biology instructor on KZfaq. Thank you so much for explaining biological concepts.

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

@tribaltumblelove 6 жыл бұрын

I have a biochemistry exam in 2 days lmao and this really helped me to understand things better. thanks mate.

@zianaqash7714 6 жыл бұрын

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@KiEfErThEnOoB 8 жыл бұрын

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@matimbantimane3170 7 жыл бұрын

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@shomusbiologyofficial 4 жыл бұрын

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@shwetaathwani4756 8 жыл бұрын

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@MultiPratik09 5 жыл бұрын

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@hinasiddiq3246 5 жыл бұрын

really helped in understanding concept of salting in and salting out.....keep working

@soumyasaxena5151 6 жыл бұрын

please upload video on different methods of protein precipitation...and this was a really helpful video.

@ahgasezrz 6 жыл бұрын

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@MissJamborina 7 жыл бұрын

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@marksteve8373 5 жыл бұрын

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@LeXaa95 7 жыл бұрын

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@shomusbiologyofficial 7 жыл бұрын

+Alexandra Gherman glad you liked it

@srrajeshmahapatra7632 7 жыл бұрын

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@shomusbiologyofficial 3 жыл бұрын

Thank you. Glad you liked my lectures

@prabirbarman1702 3 жыл бұрын

Thanks sir for this wonderful video.

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@soumikasengupta9449 6 жыл бұрын

Thankyou....It was really helpful

@rforrebel2687 3 жыл бұрын

It was awesome...thankyou sir...

@damilolaolagunju213 5 ай бұрын

Thank you very much for this explanation. I'm watching it to prep for my exam tomorrow

@shomusbiologyofficial 5 ай бұрын

All the best for future career. Stay tuned for more

@kaziazimuzzamanazim8941 3 жыл бұрын

This is Super easy to understand. Thank you❤

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@apaarnasrivastava4126 6 жыл бұрын

Awesome.....you are too good !

@demialien4770 7 жыл бұрын

this was really well explained, thank you so much! I have a question, will this precipitate all proteins without differentiation? or will it depend on the concentration of salt we add?

@sharafwasima2839 4 жыл бұрын

Thanks a lot... Now I understand it properly

@urmumification1 6 жыл бұрын

Hi from Sydney... keep the awesome work up

@vivekchamoli2063 6 жыл бұрын

Very helpful video nice.

@thatdiyguyraymondmonk1225 2 жыл бұрын

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@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@shahistha7416 7 жыл бұрын

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@shomusbiologyofficial 7 жыл бұрын

+Brave Girl Glad you liked my lectures

@ChilangaBanda08 7 жыл бұрын

Great explanation! Thank you, greetings from Houston, Texas...

@shomusbiologyofficial 7 жыл бұрын

+ChilangaBanda08 thank you. Glad you liked it

@amrutavarshinijoshi7098 6 жыл бұрын

Hey thank you this video helped me alot 🙂

@priyankakaushik5270 3 жыл бұрын

Really ur explanation out of standing.. concept clear by watching ur vedio.. Thanks alot. Please upload vedio related to refolding and protein purification so that helpful for us.

@UshaUsha-ng5cx 5 жыл бұрын

Really thank you so much sir

@leenshammout2284 4 жыл бұрын

Super helpful thank you so much 😍, Great teacher made it very easy and logical

@shomusbiologyofficial 4 жыл бұрын

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@shomusbiologyofficial 2 жыл бұрын

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@danieltapia1153 5 жыл бұрын

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@shomusbiologyofficial 4 жыл бұрын

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@shreeyashetty7514 3 жыл бұрын

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@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@G.U.M.M.M.Y 6 жыл бұрын

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@shomusbiologyofficial 3 жыл бұрын

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@khanyisilemtala1815 7 жыл бұрын

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@shomusbiologyofficial 7 жыл бұрын

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@lynamrabet2936 2 жыл бұрын

Thank you !

@asankaprageeth6245 3 жыл бұрын

thank you very much. it will very important for m for my further studies

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@nicholasjay3932 5 жыл бұрын

This video was helpful in understanding the chemistry, is there anyone out there that could provide sources applying the same information that is applicable to how salting in and salting out works with brining meats?

@sandybell234 7 жыл бұрын

Very helpful! Thank you!

@shomusbiologyofficial 7 жыл бұрын

+Sandybell Anorga thank you

@souravganguly9557 5 жыл бұрын

Really helpful for me...thank u sir...thanks a lot 😊

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@amandahogan9707 5 жыл бұрын

Super Helpful! Thank You!

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@sheikhsahar3434 7 жыл бұрын

it ws sooooooooo helpful...i never understood the difference...but after watching this...i am clear about it...thnku so much..😊

@basitazadsheikh9644 5 жыл бұрын

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@jayashreejoshi7244 3 жыл бұрын

I am a pharmacy student... It helped me with my subject... Thanks alot

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@xxvictor21xx1 5 жыл бұрын

exellent explanation my friend !!

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@mubasharkhan9795 8 жыл бұрын

thank you sir

@minalsatpute8312 4 жыл бұрын

Thank you so much.... It helped us a lot.... Looking forward for more such informative videos👍😁

@shomusbiologyofficial 4 жыл бұрын

Glad to hear that you're getting benefit from my lectures

@humairahr3401 7 жыл бұрын

Thank you Shomu :)

@Frenchfemme_Dharna 5 жыл бұрын

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@shomusbiologyofficial 5 жыл бұрын

Thank you. Glad you liked my lectures

@manicpixiedreamgirl7930 5 жыл бұрын

Hey, Thanks for this video. It's a great explanation. I just wish the sound quality was better.

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@saitej9956 4 жыл бұрын

Can you please make a video on ,in process quality control test for biological products?

@nazzisecret 5 жыл бұрын

Thank u you so much. Helped alot

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@ronaksinhparmar2288 4 жыл бұрын

thanks 4 ur efforts dear.....nly things u should have mentioned that its because of few surface hydrophobic residues that have been exposed after water been removed from dat part n this hydrophobic residues tend 2 aggregate n result in precipitation...anyway thanks agn

@orkacke 7 жыл бұрын

Great!

@Gratitude__success 6 жыл бұрын

Thank you so much

@InfiniteUniverse88 4 жыл бұрын

Are the dead sea, the great salt lake in Utah, and the Don Juan pond in Antarctica considered high salt concentrations?

@kaoutarkh8895 7 жыл бұрын

thank you so so much it was very helpfull

@shomusbiologyofficial 7 жыл бұрын

+kaoutar kh thank you. Glad it helped

@lakniehasara3208 2 жыл бұрын

Sir, After adding same amount of saturated ammonium sulphate solution to the protein sample along the wall of the test tube, what is the reason for appearing a white coloured ring in the interface?

@kayecrypto4594 2 жыл бұрын

thank you so much for this I finally understand the concept :)) 😊

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@Erram810 7 ай бұрын

Excellent explanation

@shomusbiologyofficial 7 ай бұрын

Thank you

@maryamyousaf700 4 жыл бұрын

It helps me a lot Thank u sir

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@umairbashir7049 5 жыл бұрын

Brilliant, brilliant, brilliant.

@shomusbiologyofficial 5 жыл бұрын

Thank you

@ennaragasiyam8818 6 жыл бұрын

Nice lecture....... Thank u so much.

@shomusbiologyofficial 6 жыл бұрын

+Gowsalya Shiyamala you're welcome

@manmeetsingh9936 7 жыл бұрын

awesome bro

@nba5890 3 жыл бұрын

Suggest a hypothesis to explain how increasing the salt concentration of an extract of 10nm filaments could form a solenoid. Can i get the answer of this question

@laliabenmaiza9218 2 жыл бұрын

THANK YOU VERRY MUCH

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@swatimishra8119 6 жыл бұрын

very nicely explained.

@shomusbiologyofficial 6 жыл бұрын

+Swati Mishra thank you.

@veena6586 4 жыл бұрын

Sir, is it similar like to make inside out n outside in vesicles???

@adindadvm 6 жыл бұрын

Wow thank u!

@phulmotichaudhari1900 3 жыл бұрын

Thank you sir 👌. But i m little bit confused bcz higher the molecular weight of protein,lesser the amt of salt req for precipitation?Why sir?

@babrpr Жыл бұрын

Great explanation sir❤

@shomusbiologyofficial Жыл бұрын

Thank you

@anupamanusanka7611 5 жыл бұрын

Thank u very much really understood clearly

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@niranjannayak6149 Жыл бұрын

Next level ❣️

@shomusbiologyofficial Жыл бұрын

Thank you

@zero450571 7 жыл бұрын

thank you

@Aniltharalla 3 жыл бұрын

How can we collect only one protein of intrest from salting out? As you mentioned in the video about purification of single protein by salting out but here after salting out all the protein complex mixtures will going to bind to each other. So here my question is how to extract the single protein from salting out method? Thank you

@vishalupadhyay416 Жыл бұрын

But sir denatured protein self insoluble or ptt due to hydrophobic side chains of amino acid which makes hydrophobic bonds in protein for folding and bending and this hydrophobic bond break by SDS then hydrophobic side chains expose in water which makes protein ppt due to hydrophobic interactions between protein and protein. so how does potassium acetate ppt denature protein????????? Please explain with basic details or make a video on this topic sir, please?

@kamalabuhammad164 5 жыл бұрын

You’re the best ❤️

@shomusbiologyofficial 5 жыл бұрын

Thank you. Glad you liked my lectures

@planckhouse4418 6 жыл бұрын

can u put classification of protein on derived protein

@vedanshipal1308 3 жыл бұрын

Thank you Sir!!

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@vedanshipal1308 3 жыл бұрын

@@shomusbiologyofficial Oh no!!Your reply!!🤐🤧🥺💕💕

@RiyaKumari-ft3ii Жыл бұрын

Very helpful sir

@shomusbiologyofficial Жыл бұрын

Thank you

@maheenchoudry4773 4 жыл бұрын

so informative

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@artilakra511 5 жыл бұрын

Awesome...thnk u tnk u...it helped a lot...

@shomusbiologyofficial 5 жыл бұрын

Glad to hear that you are getting benefit from the videos

@artilakra369 5 жыл бұрын

I didn't understand in clg this part....you know what,your videos helped me for my last 3 semester...really really it helped me a lot for my papers..my 4th paper mostly i studied by your videos and appeared the exam...thank u