I love you. You are the best instructor I have ever seen in my life, seriously. Thank you.
@keseiahjoytavera41412 жыл бұрын
this is what I also say whenever I see his face when I'm finding lectures. lmao. I love this man. ALL RESPECT
@fabiomingione69788 жыл бұрын
This is an excellent lecture. I found this waaaaay more helpful than my teacher's explanation in my native language. I'm surprised of this great work :) Good job.
@anapaolarv7 жыл бұрын
If I pass my final Enzymology exam tomorrow, i owe you everything.
@mallakaljarawen8885 жыл бұрын
You’ve explained this much better than my professor that had studied in Harvard University . I really thank you
@irineandrona79486 жыл бұрын
It's like magic!!! I mean in just 10 minutes I understand more than I would in a three hours lecture! ! If I ever take a degree it would be mostly because of you
@holly83305 жыл бұрын
Thank you so much for getting me through biochemistry and anatomy. Have watched sooo many of your videos and they ALWAYS help!!!
@Mycatnana2 жыл бұрын
Whenever someone try to teach they all talk about complex mechanics which is very hard to catch when studying the new concept but in your case it's so easy to understand the basic of the topic...
@palaksangal68227 жыл бұрын
Your lectures have made studying easier and concepts clear. Excellent lectures.
@lakep77982 жыл бұрын
Your analogies are EXCELLENT! Thank you! You may possibly be the best teacher EVER.
@roshanaatreyapaudel40335 жыл бұрын
Beatle and ant example u have given in this lecture can explain this chromatography in a best way👏👏👏
@alaa25812 жыл бұрын
thank you so much For great explanation especially as Egyptian my English is not good enough so when you speak clearly and slowly it really helps
@Mycatnana2 жыл бұрын
Omg u blew up my mind....english is not my first language but I understand bit by bit what u just taught.....very simple to understand...thanks a lot🙃🙃🙃
@sumonagarg34287 жыл бұрын
the best teacher seriously. you explain it so well sir. that helped me a lot. thankyou
@cookies36915 жыл бұрын
getting my BSc degree thanks to this guy.
@sadiabinte82813 жыл бұрын
I really think this Ak lectures tutorials are like gems to all student 🖤
@gowrishvaka63566 жыл бұрын
Really didn't use you much for organic chemistry, but using you right now for biochemistry. Your videos are amazing sir.
@Aminehordy9 жыл бұрын
I did not understand the lesson in the classroom , but here *-* the way that you explain is very usefull , thank's a lot
@AKLECTURES9 жыл бұрын
Aminehordy glad to hear that mate :)
@dstan162244 жыл бұрын
Thank you very much Sir...You are undoubtedly one of the finest teachers I have ever learned from...
@z_binshaibah9 жыл бұрын
I have a chromatography report to write and this was so helpful thank you so much :>
@AKLECTURES9 жыл бұрын
zazooq88 awesome! you're welcome :)
@mohammedahmedjalloh5314 жыл бұрын
Basically watching this 1hr to laboratory quiz 🤗🤗🤗 how I feel about your lecture right now
@aishamapnoo46298 жыл бұрын
i love ur way of presenting lectures.. thank you v much for all the help..
@astro81496 жыл бұрын
your diagrams are always so clean and detailed damn i wish i could draw that nicely
@eddymutugi56952 жыл бұрын
Good Explanation Sir I Loved It and Every Video you post They are just So Simple to understand
@maryc59059 жыл бұрын
very clear explanations - thank you so much for posting this lecture!
@mountcarmel71163 жыл бұрын
i can not say thank you enough for your hard working wonderful explanations.
@azharrashid61067 жыл бұрын
surely a best explanation.... thank you sir!!☺️☺️
@hudamh74797 жыл бұрын
I love the way you present your lecture wish you all the best
@nanjehelgabemoh28743 жыл бұрын
Thank you very much. Your explanations are very helpful
@chiragmohansharma9 жыл бұрын
awesome work!! great explanation right from the basics!!
@zylia58072 жыл бұрын
Thank you so much sir your videos are really helpful. Also you explain way better than my professor 😍
Thank you so much. I always enjoy your explanation. God bless you
@catherinsebastian81623 жыл бұрын
You are a Real Game Changer 💕 dear 💝🤗
@SK-gj7vh8 ай бұрын
Best teacher🙌🙌🙌
@RaiSaysS2 жыл бұрын
Wonderful explanation!!!!!! keep it up!!!!!
@iliasboukrach1727 жыл бұрын
You are a great teacher !!! keep on going !!
@amitalenka61473 жыл бұрын
Best explanation!
@kendrapacho7272 жыл бұрын
Thank YOUUUUUU SOOO MUCH for all you DO.
@JBOfficial.992 ай бұрын
Thank you Boss for making me to understand this
@Eric-sq4hd4 жыл бұрын
You are such a champion. Total BOSS.
@nanazamini7188Ай бұрын
Some universities require to take biochem extended program , as USC, and u should pay 2000 dollars by ur own pocket and barely teaching u something literally their professor don’t know anything thank u Andrey u r here I love ur videos
@AKLECTURESАй бұрын
thank you!
@sahildhindsa87632 жыл бұрын
AWESOME video. Thank you so much.
@palakarora19793 жыл бұрын
Amazing explanation.. Thank you
@lujain975 жыл бұрын
Very clear explanations , thank you soo much 🧬
@junczhang8 жыл бұрын
best lecturer everrrr
@pitbulllover704 жыл бұрын
you are seriously the best!
@pinksweet587 жыл бұрын
Thank You so much!!! these videos are so helpful!
@karatekidmom7 жыл бұрын
So on a SEC machine, buffer is supposed to be constantly pumped through the system. What is the consequence if there is not a consistent flow of the buffer? This happened during a portion of our experiment and some overlapping peaks showed on the chromatogram.
@mevlutakdag39476 жыл бұрын
Brilliant! I am not a native speaker but i really got it well. But i have a question. I wonder if someone can regain the proteins in a 100% purity or a near percentage purity by using this method. Thank you sir for these wonderful lectures.
@idrisd526 жыл бұрын
awesome explanation sir...helped alot
@jaxsoncarson78367 жыл бұрын
Why doesn't the protein of interest dissolve back into the solution in the baggie, and do the other proteins that were in the baggie go through the semipermeable membrane? And if they do then why wouldn't the protein of interest also go through since salt concentration in the baggie is going down making it soluble in water?
@noorb.84377 жыл бұрын
This is so helpful ! Thank you .
@yehong50554 жыл бұрын
best lecture
@benjaminnrgaard52662 жыл бұрын
Great video, keep it up! :))
@MrsCollVani8 жыл бұрын
Excellent tutorial..thankyou!!!!!
@arlethtorija80473 ай бұрын
amazing video , thank you so muchh
@jasonruiz92967 жыл бұрын
Thank you so much for this! Watching this video truly helped me fortify a few ideas for my lab report :)
@moseshayab84975 жыл бұрын
Thank you very much. This was so clear.
@kareemjeiroudi19646 жыл бұрын
Best teacher
@nwanduchikeluba40943 жыл бұрын
You are the best. How about separation of polysaccharides? And I'd like to also emphasise that some molecules bear same color, in that case what will one do about timing?
@compactdisk27534 жыл бұрын
Just a question, is there a factor that forces the small proteins to enter the pore? I mean isn't there also a chance that the small proteins won't enter the pore and just follow the same pathway that the larger proteins will take?
@arunkumars6257 Жыл бұрын
Thank you so much!
@kellynkells6630 Жыл бұрын
If the proteins under study were not coloured, what alternative method could be used to monitor the fractionation process?
@kasondebbuku6487 Жыл бұрын
Thank you.
@clairelee72649 жыл бұрын
informative and easy explanation. Thanks!
@AKLECTURES9 жыл бұрын
you're welcome! :)
@mimakue11777 жыл бұрын
best explanation eeeeeveeer thank you
@lostinthoughts135 жыл бұрын
This is really good.
@olgafahy96427 жыл бұрын
that was so helpful, thank you!
@ziadatalla37274 жыл бұрын
this is gold
@Lucerozeus7 жыл бұрын
Soon I will be able to give back. Thank you so much!
@koreanshots67166 жыл бұрын
One question I want to ask --where the mobile phase is...we have the stationary phase,mixture to be separated but mobile phase?
@ghiwanassereddine34069 жыл бұрын
THANKYOU!! very understandable!
@ka14583 жыл бұрын
thank you vary vary much for that
@rehabalsaleh1665 жыл бұрын
You are great!
@moameneltokhy14178 жыл бұрын
Well explained
@sarangas86883 жыл бұрын
thank you sir
@humbleresearcher9 жыл бұрын
hi, thanks for the lecture it really helps.But i have a queston to ask, and I never do any protein purification work. So , I want to ask about the tsk gel filtration, how much volume does it require to run this method? And, can I get the molecular weight of the protein that I'm using using this method?
@NeirinCedric7 жыл бұрын
Couldn't possibly be clearer
@ironman7187 жыл бұрын
thank you man your awesome..
@dwightsbeats42747 жыл бұрын
What is your day job? Also, have you obtained any degrees? Just curious as you seem to know about a lot of different aspects of certain science topics.
@gokulkrishna74118 жыл бұрын
thank you very much
@jwilliams54069 жыл бұрын
great stuff, thank you!
@AKLECTURES9 жыл бұрын
J Williams you're welcome!
@shahdismailalqatu97116 жыл бұрын
very helpful :) thank you .
@ibraalsabbah29709 жыл бұрын
thank you ,,, you are the best :)
@lyliat3548 жыл бұрын
Thank you Teacher :D !
@veliborcabarkapa42715 жыл бұрын
excellent
@alexanderzwier6487 жыл бұрын
Very good but why do you pour water on top ? is it simply to push the protein down?
@HStiglitzz7 жыл бұрын
Quirijn Zwier i think this is the reason why he pour water, but i can be wrong
@vijetamahapatra95107 жыл бұрын
excellent !!!!!
@timothyfranklin37067 жыл бұрын
good jobs
@eyaguedhami42138 жыл бұрын
why is there a variation of volume when the protein emerge ??
@verooobaaby6 жыл бұрын
What is the difference between Gel Filtration Chromatography and Size Exclusion Chromatography?
@manjitrana36086 жыл бұрын
Basically the names are used interchangeably. However, to be more specific Gel Filtration Chromatography is one of the methods of Size Exclusion Chromatography. Size Exclusion Chromatography is an umbrella term. Other methods include Gel Permeation Chromatography where you use organic solvent as a mobile phase rather than aqueous solvent as in Gel Filtration Chromatography. Hope that helps!
@maryamjamal65389 жыл бұрын
Can you help me..I need some questions and their answer about this subject (chromatography ...electrophoresis)
@janthonymorris43949 жыл бұрын
Do you have any videos about ubiquination of proteins?
@sreevidyatanjavuru70499 жыл бұрын
thank u sir
@DesignerReviewz8 жыл бұрын
You suck at lecturing!!! Just kidding. Thanks for the concise explanation. You have a new subscriber!
@victorma93554 жыл бұрын
wait is this related to column chromatography?
@ooiTzAmii7 жыл бұрын
What can you do with the collected fractions?
@melodylund92498 жыл бұрын
Hi. Why purple color indicates the presence of protein?
@NiaBalcer7 жыл бұрын
its probably either the natural colour of the protein or it's dyed to make observations easier
@victorma93554 жыл бұрын
can i use silica gel
@PerunMorph8 жыл бұрын
What would happen, if we have, 3 proteins of a large difference in size, but all are too big to fit the pores, will they all come out at the same time?
@NiaBalcer7 жыл бұрын
you choose a bigger pore size... one that allows one of the molecules while keeping out the other
@PerunMorph7 жыл бұрын
Not what I asked.
@mugdhan14157 жыл бұрын
+PerunMorph there are different size of gels beads which can be used is what he meant !
@PerunMorph7 жыл бұрын
I know what he was trying to say, but that is not the answer to my question.
@uhuihiuihiuh30427 жыл бұрын
Yes, the gel has a fractionation range. Proteins within this range will separate, while proteins outside this range will not. The 3 large proteins in your question would elute together as one molecule and therefore at the same time. Similarly, proteins that are too small to separate will fail to separate and also elute as a single molecule. However, if you have 3 proteins that are too large and 3 proteins that are too small, the 3 proteins that are too large will elute faster than the 3 that are too small, even though they are all outside the fractionation range.