Gel Filtration Chromatography

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Gel Filtration Chromatography

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Күн бұрын

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@Funnyguy992 8 жыл бұрын

I love you. You are the best instructor I have ever seen in my life, seriously. Thank you.

@keseiahjoytavera4141 2 жыл бұрын

this is what I also say whenever I see his face when I'm finding lectures. lmao. I love this man. ALL RESPECT

@fabiomingione6978 8 жыл бұрын

This is an excellent lecture. I found this waaaaay more helpful than my teacher's explanation in my native language. I'm surprised of this great work :) Good job.

@anapaolarv 7 жыл бұрын

If I pass my final Enzymology exam tomorrow, i owe you everything.

@mallakaljarawen888 5 жыл бұрын

You’ve explained this much better than my professor that had studied in Harvard University . I really thank you

@irineandrona7948 6 жыл бұрын

It's like magic!!! I mean in just 10 minutes I understand more than I would in a three hours lecture! ! If I ever take a degree it would be mostly because of you

@holly8330 5 жыл бұрын

Thank you so much for getting me through biochemistry and anatomy. Have watched sooo many of your videos and they ALWAYS help!!!

@Mycatnana 2 жыл бұрын

Whenever someone try to teach they all talk about complex mechanics which is very hard to catch when studying the new concept but in your case it's so easy to understand the basic of the topic...

@palaksangal6822 7 жыл бұрын

Your lectures have made studying easier and concepts clear. Excellent lectures.

@lakep7798 2 жыл бұрын

Your analogies are EXCELLENT! Thank you! You may possibly be the best teacher EVER.

@roshanaatreyapaudel4033 5 жыл бұрын

Beatle and ant example u have given in this lecture can explain this chromatography in a best way👏👏👏

@alaa2581 2 жыл бұрын

thank you so much For great explanation especially as Egyptian my English is not good enough so when you speak clearly and slowly it really helps

@Mycatnana 2 жыл бұрын

Omg u blew up my mind....english is not my first language but I understand bit by bit what u just taught.....very simple to understand...thanks a lot🙃🙃🙃

@sumonagarg3428 7 жыл бұрын

the best teacher seriously. you explain it so well sir. that helped me a lot. thankyou

@cookies3691 5 жыл бұрын

getting my BSc degree thanks to this guy.

@sadiabinte8281 3 жыл бұрын

I really think this Ak lectures tutorials are like gems to all student 🖤

@gowrishvaka6356 6 жыл бұрын

Really didn't use you much for organic chemistry, but using you right now for biochemistry. Your videos are amazing sir.

@Aminehordy 9 жыл бұрын

I did not understand the lesson in the classroom , but here *-* the way that you explain is very usefull , thank's a lot

@AKLECTURES 9 жыл бұрын

Aminehordy glad to hear that mate :)

@dstan16224 4 жыл бұрын

Thank you very much Sir...You are undoubtedly one of the finest teachers I have ever learned from...

@z_binshaibah 9 жыл бұрын

I have a chromatography report to write and this was so helpful thank you so much :>

@AKLECTURES 9 жыл бұрын

zazooq88 awesome! you're welcome :)

@mohammedahmedjalloh531 4 жыл бұрын

Basically watching this 1hr to laboratory quiz 🤗🤗🤗 how I feel about your lecture right now

@aishamapnoo4629 8 жыл бұрын

i love ur way of presenting lectures.. thank you v much for all the help..

@astro8149 6 жыл бұрын

your diagrams are always so clean and detailed damn i wish i could draw that nicely

@eddymutugi5695 2 жыл бұрын

Good Explanation Sir I Loved It and Every Video you post They are just So Simple to understand

@maryc5905 9 жыл бұрын

very clear explanations - thank you so much for posting this lecture!

@mountcarmel7116 3 жыл бұрын

i can not say thank you enough for your hard working wonderful explanations.

@azharrashid6106 7 жыл бұрын

surely a best explanation.... thank you sir!!☺️☺️

@hudamh7479 7 жыл бұрын

I love the way you present your lecture wish you all the best

@nanjehelgabemoh2874 3 жыл бұрын

Thank you very much. Your explanations are very helpful

@chiragmohansharma 9 жыл бұрын

awesome work!! great explanation right from the basics!!

@zylia5807 2 жыл бұрын

Thank you so much sir your videos are really helpful. Also you explain way better than my professor 😍

@otimdenishokori6703 2 жыл бұрын

Wonderful,,,,, great,,, perfect,,, fantastic lecture,,,, excellent

@bakareolamide4371 3 жыл бұрын

Thank you so much. I always enjoy your explanation. God bless you

@catherinsebastian8162 3 жыл бұрын

You are a Real Game Changer 💕 dear 💝🤗

@SK-gj7vh 8 ай бұрын

Best teacher🙌🙌🙌

@RaiSaysS 2 жыл бұрын

Wonderful explanation!!!!!! keep it up!!!!!

@iliasboukrach172 7 жыл бұрын

You are a great teacher !!! keep on going !!

@amitalenka6147 3 жыл бұрын

Best explanation!

@kendrapacho727 2 жыл бұрын

Thank YOUUUUUU SOOO MUCH for all you DO.

@JBOfficial.99 2 ай бұрын

Thank you Boss for making me to understand this

@Eric-sq4hd 4 жыл бұрын

You are such a champion. Total BOSS.

@nanazamini7188 Ай бұрын

Some universities require to take biochem extended program , as USC, and u should pay 2000 dollars by ur own pocket and barely teaching u something literally their professor don’t know anything thank u Andrey u r here I love ur videos

@AKLECTURES Ай бұрын

thank you!

@sahildhindsa8763 2 жыл бұрын

AWESOME video. Thank you so much.

@palakarora1979 3 жыл бұрын

Amazing explanation.. Thank you

@lujain97 5 жыл бұрын

Very clear explanations , thank you soo much 🧬

@junczhang 8 жыл бұрын

best lecturer everrrr

@pitbulllover70 4 жыл бұрын

you are seriously the best!

@pinksweet58 7 жыл бұрын

Thank You so much!!! these videos are so helpful!

@karatekidmom 7 жыл бұрын

So on a SEC machine, buffer is supposed to be constantly pumped through the system. What is the consequence if there is not a consistent flow of the buffer? This happened during a portion of our experiment and some overlapping peaks showed on the chromatogram.

@mevlutakdag3947 6 жыл бұрын

Brilliant! I am not a native speaker but i really got it well. But i have a question. I wonder if someone can regain the proteins in a 100% purity or a near percentage purity by using this method. Thank you sir for these wonderful lectures.

@idrisd52 6 жыл бұрын

awesome explanation sir...helped alot

@jaxsoncarson7836 7 жыл бұрын

Why doesn't the protein of interest dissolve back into the solution in the baggie, and do the other proteins that were in the baggie go through the semipermeable membrane? And if they do then why wouldn't the protein of interest also go through since salt concentration in the baggie is going down making it soluble in water?

@noorb.8437 7 жыл бұрын

This is so helpful ! Thank you .

@yehong5055 4 жыл бұрын

best lecture

@benjaminnrgaard5266 2 жыл бұрын

Great video, keep it up! :))

@MrsCollVani 8 жыл бұрын

Excellent tutorial..thankyou!!!!!

@arlethtorija8047 3 ай бұрын

amazing video , thank you so muchh

@jasonruiz9296 7 жыл бұрын

Thank you so much for this! Watching this video truly helped me fortify a few ideas for my lab report :)

@moseshayab8497 5 жыл бұрын

Thank you very much. This was so clear.

@kareemjeiroudi1964 6 жыл бұрын

Best teacher

@nwanduchikeluba4094 3 жыл бұрын

You are the best. How about separation of polysaccharides? And I'd like to also emphasise that some molecules bear same color, in that case what will one do about timing?

@compactdisk2753 4 жыл бұрын

Just a question, is there a factor that forces the small proteins to enter the pore? I mean isn't there also a chance that the small proteins won't enter the pore and just follow the same pathway that the larger proteins will take?

@arunkumars6257 Жыл бұрын

Thank you so much!

@kellynkells6630 Жыл бұрын

If the proteins under study were not coloured, what alternative method could be used to monitor the fractionation process?

@kasondebbuku6487 Жыл бұрын

Thank you.

@clairelee7264 9 жыл бұрын

informative and easy explanation. Thanks!

@AKLECTURES 9 жыл бұрын

you're welcome! :)

@mimakue1177 7 жыл бұрын

best explanation eeeeeveeer thank you

@lostinthoughts13 5 жыл бұрын

This is really good.

@olgafahy9642 7 жыл бұрын

that was so helpful, thank you!

@ziadatalla3727 4 жыл бұрын

this is gold

@Lucerozeus 7 жыл бұрын

Soon I will be able to give back. Thank you so much!

@koreanshots6716 6 жыл бұрын

One question I want to ask --where the mobile phase is...we have the stationary phase,mixture to be separated but mobile phase?

@ghiwanassereddine3406 9 жыл бұрын

THANKYOU!! very understandable!

@ka1458 3 жыл бұрын

thank you vary vary much for that

@rehabalsaleh166 5 жыл бұрын

You are great!

@moameneltokhy1417 8 жыл бұрын

Well explained

@sarangas8688 3 жыл бұрын

thank you sir

@humbleresearcher 9 жыл бұрын

hi, thanks for the lecture it really helps.But i have a queston to ask, and I never do any protein purification work. So , I want to ask about the tsk gel filtration, how much volume does it require to run this method? And, can I get the molecular weight of the protein that I'm using using this method?

@NeirinCedric 7 жыл бұрын

Couldn't possibly be clearer

@ironman718 7 жыл бұрын

thank you man your awesome..

@dwightsbeats4274 7 жыл бұрын

What is your day job? Also, have you obtained any degrees? Just curious as you seem to know about a lot of different aspects of certain science topics.

@gokulkrishna7411 8 жыл бұрын

thank you very much

@jwilliams5406 9 жыл бұрын

great stuff, thank you!

@AKLECTURES 9 жыл бұрын

J Williams you're welcome!

@shahdismailalqatu9711 6 жыл бұрын

very helpful :) thank you .

@ibraalsabbah2970 9 жыл бұрын

thank you ,,, you are the best :)

@lyliat354 8 жыл бұрын

Thank you Teacher :D !

@veliborcabarkapa4271 5 жыл бұрын

excellent

@alexanderzwier648 7 жыл бұрын

Very good but why do you pour water on top ? is it simply to push the protein down?

@HStiglitzz 7 жыл бұрын

Quirijn Zwier i think this is the reason why he pour water, but i can be wrong

@vijetamahapatra9510 7 жыл бұрын

excellent !!!!!

@timothyfranklin3706 7 жыл бұрын

good jobs

@eyaguedhami4213 8 жыл бұрын

why is there a variation of volume when the protein emerge ??

@verooobaaby 6 жыл бұрын

What is the difference between Gel Filtration Chromatography and Size Exclusion Chromatography?

@manjitrana3608 6 жыл бұрын

Basically the names are used interchangeably. However, to be more specific Gel Filtration Chromatography is one of the methods of Size Exclusion Chromatography. Size Exclusion Chromatography is an umbrella term. Other methods include Gel Permeation Chromatography where you use organic solvent as a mobile phase rather than aqueous solvent as in Gel Filtration Chromatography. Hope that helps!

@maryamjamal6538 9 жыл бұрын

Can you help me..I need some questions and their answer about this subject (chromatography ...electrophoresis)

@janthonymorris4394 9 жыл бұрын

Do you have any videos about ubiquination of proteins?

@sreevidyatanjavuru7049 9 жыл бұрын

thank u sir

@DesignerReviewz 8 жыл бұрын

You suck at lecturing!!! Just kidding. Thanks for the concise explanation. You have a new subscriber!

@victorma9355 4 жыл бұрын

wait is this related to column chromatography?

@ooiTzAmii 7 жыл бұрын

What can you do with the collected fractions?

@melodylund9249 8 жыл бұрын

Hi. Why purple color indicates the presence of protein?

@NiaBalcer 7 жыл бұрын

its probably either the natural colour of the protein or it's dyed to make observations easier

@victorma9355 4 жыл бұрын

can i use silica gel

@PerunMorph 8 жыл бұрын

What would happen, if we have, 3 proteins of a large difference in size, but all are too big to fit the pores, will they all come out at the same time?

@NiaBalcer 7 жыл бұрын

you choose a bigger pore size... one that allows one of the molecules while keeping out the other

@PerunMorph 7 жыл бұрын

Not what I asked.

@mugdhan1415 7 жыл бұрын

+PerunMorph there are different size of gels beads which can be used is what he meant !

@PerunMorph 7 жыл бұрын

I know what he was trying to say, but that is not the answer to my question.

@uhuihiuihiuh3042 7 жыл бұрын

Yes, the gel has a fractionation range. Proteins within this range will separate, while proteins outside this range will not. The 3 large proteins in your question would elute together as one molecule and therefore at the same time. Similarly, proteins that are too small to separate will fail to separate and also elute as a single molecule. However, if you have 3 proteins that are too large and 3 proteins that are too small, the 3 proteins that are too large will elute faster than the 3 that are too small, even though they are all outside the fractionation range.