This video shows the typical way to carry out a solid phase extraction process (SPE). More specifically, a molecularly imprinted polymer AFFINIMIP SPE BISPHENOL A was used for this video.
Solid-phase extraction (SPE) is a sample preparation technique routinely used in analytical laboratories for the extraction of analytes from a complex matrix.
This sample preparation technique enables the extraction, cleanup and concentration of analytes prior to their quantification. Solid phase extraction prevents most problems encountered with liquid-liquid extraction and improves quantitative recovery yields. This technique is rapid (most extraction in less than 30 min), easy to perform and can be automated. In addition, low amount of solvents is handled and this technique is fully adapted for the pre-treatment of complex matrices such as urine, blood, food samples, water etc...
SPE procedure:
A typical solid phase extraction involves four steps:
1- The cartridge is equilibrated or conditioned with a solvent to wet the sorbent.
2- The loading solution containing the analyte is percolated through the solid phase. Ideally, the analyte and some impurities are retained on the sorbent.
3- The sorbent is then washed to remove impurities.
4- The analyte is collected during this elution step.
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