Solid Phase Extraction (SPE) Sample Preparation

Solid Phase Extraction (SPE) Sample Preparation - Fundamentals

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Күн бұрын

The fundamentals of Solid Phase Extraction (SPE) sample preparation, including choosing the correct SPE format and using the four basic SPE steps, are discussed. Choosing the appropriate format for your SPE will be based on the analyte, matrix, volume and concentration of your sample. The five basic formats for SPE products, including the straight barrel or syringe barrel, large reservoir capacity (LRC), Bond Elut Jr., 96-well plate, and pipette tip, are discussed. There are four basic steps to SPE. CLWE stands for Condition, Load, Wash, Elute. Conditioning is usually a two-step process and is used to prepare the sorbent for extraction. The Loading step is used to load your sample. Be sure to understand your matrix and basic characteristics associated with your compound(s) of interest. Washing refers to using a solvent or solution to wash interferences from the SPE material, without removing the compounds of interest. Elution refers to the solvent required to elute the compounds of interest from the SPE material.
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Пікірлер: 29

@abmackay 6 жыл бұрын

"Ben, what are your thoughts about conditioning?" "In order to have glossy, flowing hair like mine, I recommend a creme rinse with added botanical protein." "Dammit Ben."

@justme3888 7 жыл бұрын

I was looking for videos on blood banking solid phase testing and ended up here. Watched the whole video anyway 10/10.

@JC-vu6sn Жыл бұрын

Excellent video. Great information

@noushinnasiri8029 7 жыл бұрын

Thanks! Great video!

@aasmabibi208 2 жыл бұрын

very nice and very informative, thanks

@ucjakozicka978 10 ай бұрын

this is brilliant, thank you!

@fasrinmahe 7 жыл бұрын

well done

@Analikol 7 жыл бұрын

Thanks a lot!

@TaliaOutwrong 7 жыл бұрын

Well said Ben!

@charnaeyoung9815 6 жыл бұрын

Talia Enright lmao

@HuiHui-hq5sf 7 жыл бұрын

thank you so much

@agustinamatticoli57 7 жыл бұрын

thank you!

@agilent 7 жыл бұрын

Welcome! :)

@PrakashPulse4573 2 жыл бұрын

Thank you very much

@silviassk9070 7 жыл бұрын

thanks

@najibrashid2063 3 жыл бұрын

Thank you

@annasreza5253 5 жыл бұрын

Mantap

@blackyme2889 3 жыл бұрын

thank you frol algeria

@brightenvirotech6584 Жыл бұрын

Please use those critical points in background, either with board or slides. It's difficult to remember and recall And don't forget that your video is being watched by people like us, not the experts. And yes, it's good.

@ruelcedeno1376 5 жыл бұрын

What's the difference between solid phase extraction and HPLC?

@leehaiko3999 3 жыл бұрын

Usually Solid Phase Extraction is performed prior to gc/hplc to increase the concentration of the analyte of interest or to remove any contaminants or interference that can be in the sample.