Southern Blotting- Principle, Procedure and Applications

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10 жыл бұрын

Notes on Southern Blotting- Principle, Procedure and Applications
www.biologyexams4u.com/2013/12...
Southern Blotting Steps
www.biologyexams4u.com/2013/12...

Пікірлер: 44

@arunimaviswanathan4316 6 жыл бұрын

Very well explained ! Good work

@kavithanair1527 8 жыл бұрын

very simple and easy to understand...... thank u...

@user-iz2kl3fo9x 3 ай бұрын

Thank u so much brother/sir 🥺🙌❣️

@aboutstudies123 6 жыл бұрын

Thank you Sir. Very nice explanation

@malv4935 8 жыл бұрын

Thank you so much! It explained it perfectly.

@sindhusew 4 жыл бұрын

@amruthaammu1503 7 жыл бұрын

very useful...and thank u !!!

@sanaariaz6 8 жыл бұрын

thank you 😊

@priyankabya2092 3 жыл бұрын

Sir! What are the requisites for this southern blotting practical?

@beingcreative7817 Жыл бұрын

Love from India... Thanks for your efforts..

@biologyexams4u Жыл бұрын

So nice of you..... Love from India too

@161-shubhangiprajapati8 2 ай бұрын

❤❤❤❤

@Tamchrang Ай бұрын

It helps me so much thank you

@biologyexams4u Ай бұрын

Glad it was helpful... Thank you

@arunabhparkhi2008 7 жыл бұрын

Thankz...

@sheebakhan6027 9 жыл бұрын

sound too low to hear improve sound quality

@drsaharabdulkarim8996 7 жыл бұрын

helped me a lot at least to know an overall procedure and continue my search .....thank you so much sir

@rushabhlovesu 8 жыл бұрын

Very helpful...thank you!..

@biologyexams4u 8 жыл бұрын

Really appreciate your nice words. Really a motivation to do more.....Thank you so much for the visit and comment

@leonardobuback4408 9 жыл бұрын

Hello, First of all, nice video. Second, i have a question: I'd like to know how sensible are electroforesis in distinguishing DNA fragments by size. Would I be able to notice any diference between a fragment of 1 kb and a fragment of 2 kb? Thank you

@JC-vi6rc 9 жыл бұрын

Leonardo Buback Yes, a 1kb difference in DNA fragments would be easily resolved by gel electrophoresis. I think a 1% gel (1g agarose powder per 100 mL 1X TAE), ran at ~100 volts for 30-45 minutes would work beautifully. You would run a ladder to gauge the sizes of bands based on their migration. I would use the 1kb ladder from New England Biolabs.

@chandinigupta2766 9 жыл бұрын

How is the nitrocellulose membrane helping in the hybridization? Why is it used? Can't it be done directly on the gel?

@hershcheriyil6148 2 жыл бұрын

This is a speciall Membranous sheet where molecules can be attached gel can't be used ahead . In southern blotting we find a gene of interest from target dna for which addition of probe is required n then additional process like autoradiography ( incase of radio active probe) where how will you use this gel? If you are familiarize with how initially we loaded the dna you would understand it's not practical and i don't think it's possible for probe to run over gel again.

@sindhusew 4 жыл бұрын

@ghema860 2 жыл бұрын

Thank u sir 👍

@biologyexams4u 2 жыл бұрын

Hi Hema Most welcome...All the best Just sharing a related video for your reference kzfaq.info/get/bejne/p9CobKycspbDXY0.html Please consider subscribing biologyexams4u. Thank you so much for your support, Stay Safe and Stay Blessed:)

@tanisakarar8286 2 жыл бұрын

💜💜💜💜💜

@biologyexams4u 2 жыл бұрын

Thank you:)

@sujaygowda4358 2 жыл бұрын

Bro, is colonial hybridization and southern blotting is same or different?

@biologyexams4u 2 жыл бұрын

Brother Colony hybridization is used to find a specific DNA within a bacterial colony Southern blotting is used to find a specific DNA fragment in a isolated DNA sample The principle of both procedure is same that is hybridization Thank you:)

@sujaygowda4358 2 жыл бұрын

@@biologyexams4u tq bro

@marcushooo 10 жыл бұрын

this is a great video, very informational. but it would be better if you did the same video in English instead.

@biologyexams4u 10 жыл бұрын

Hi marcushoo, Thank you for the visit and comment. I think, language is not a barrier if our intention is to share our knowledge. Hope you got something.....

@Pink_kiwi707 9 жыл бұрын

mho I understood