Two-Dimensional Gel Electrophoresis

Рет қаралды 124,294

9 жыл бұрын

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Пікірлер: 86

@MrMetalHead1100 6 жыл бұрын

One of the best explanations Ive heard on youtube for any molecular biology technique. Great Job!

@nadinecampbell3717 7 жыл бұрын

Love you. Just pure brilliance across such a vast field. Bravo!

@jitukumsa225 9 жыл бұрын

Never ever stop making videos. Thank you so much.

@rainajung185 7 жыл бұрын

Omg your lectures are awesome! Ive been watching all the gel electrophoresis videos and its so clear and also in details i dnt have to waste all my time looking for info on the internet anymore. Thank you!!

@medinlab141 3 жыл бұрын

How can you be always so clear? I love your videos!

@angadmahanta8807 5 жыл бұрын

the main objective of all the aklectures videos is to get all your basics and concepts clear

@RachySarahForver 6 жыл бұрын

This man deserves my degree.

@LuisRomero-mj6td 6 жыл бұрын

Thank you, it was a good explanation. Please keep working, there are so many people that need it.

@harshvardhansingh1240 7 жыл бұрын

simply thank you, you are the best teacher in the universe, you make things so easy , your every vedio is amazing!!, thank you for making us so knowledgeable

@astradaniels 8 жыл бұрын

This is very helpful for helping me understand material in my molecular biotechnology class.

@arlsffa 8 жыл бұрын

U have no idea how much You´ve been helping me !! Thank You soooo much ! Since I found you, I fell in love with Biochem again lol

@PreetKaur-fk3ds 6 жыл бұрын

It is just unmatched... Thank you thank you thank you a million

@alexandremondaini 8 жыл бұрын

Congrtulations for your great work and easily understandable teaching !!!

@vikcheban923 4 жыл бұрын

What a clever way to distinguish between different types of proteins

@jaydeepadhikari 7 жыл бұрын

This is so awesomely exlained.. Thank you very much!!!

@rashi715 8 жыл бұрын

very helful videos ... thank u ... simple explainations to tough topics....

@raquelmorellkessler3029 3 жыл бұрын

Amazing explanation I understand everything with your Videos!!

@shijimasorce 8 жыл бұрын

Thank you for this. You do a much better job at explaining this than my professor. Likewise with a few other videos you have made. I just subscribed and gave your videos a thumbs up. :)

@mekz_n3505 2 жыл бұрын

you are an absolute legend man

@rishimakhanlal8905 7 жыл бұрын

Best explanation of 2D-PAGE!!

@hajard6828 8 жыл бұрын

very helpful, thank you so much!

@themussseee Жыл бұрын

Wonderful lecture finally understood this

@AmalDhivaharSURBT 4 жыл бұрын

This is brilliant. Thank you.

@jiaronglin8693 8 жыл бұрын

You Are the BEST! ALWAYS!

@MisssCooCo 3 жыл бұрын

Omg! You are a life saver! Thank you so much!

@pavitrabhat9978 2 жыл бұрын

Very Helpful.. Thank you so much sir..

@KingDanny1123 9 жыл бұрын

Thank you so much! very clear explanation!

@AKLECTURES 9 жыл бұрын

tammy wong you're welcome Tammy :)

@suerose8297 2 жыл бұрын

thank you so much, because this video I really understand this topic.😊

@xRay5454 Жыл бұрын

Excellent video

@ennaidevadla 7 жыл бұрын

I learned a lot. Thanks!

@teeteebaby09 8 жыл бұрын

You are AMAZING! THANK YOU!

@adindakadar9239 7 жыл бұрын

I love this. I love you. Thank youuuu!

@thehindureturn2339 3 жыл бұрын

Thank you very much sir 💝 My concept is clear Love from INDIA # BHARAT

@ridsbutterfly9850 7 жыл бұрын

you made it too easy.. thanks

@chaimataifi8799 2 жыл бұрын

Very helpful , thanks a lot teacher.

@jeannezhou5949 6 жыл бұрын

very clear, Thank you VERY MUCH.

@MrMetalHead1100 6 жыл бұрын

Im assuming after you run the 2D gel, you can then do a westernblot as you would with a typical SDS gel and then treat with antibodies to determine different post translational modifications of a protein for example?

@Ku7zu3 11 ай бұрын

Thank u this video very helpful

@joannaelhaj3571 3 жыл бұрын

Thank you very much for your video. I have a quick question, when the pI is added to the SDS page, will the mass and size increase as you’re going down the SDS page?

@naomimankrado8510 7 жыл бұрын

Where will I be without your videos? Thank you !

@siloPIRATE 6 жыл бұрын

I ould have failed without this channel and the channelShomu's Biology

@charlesndandala9009 2 жыл бұрын

Thank you, useful.

@yaseminacar4329 8 жыл бұрын

Thank you for this clear teaching. This video helped me for preparing my exam :)

@asishswain1259 4 жыл бұрын

Which exam ?

@yaseminacar4329 4 жыл бұрын

@@asishswain1259 Genomics and proteomics

@asishswain1259 4 жыл бұрын

@@yaseminacar4329 Are you doing Ph.D in genomics ?

@yaseminacar4329 4 жыл бұрын

@@asishswain1259 it was my undergraduate exam. I am doing PhD in molecular pharmacology

@kathrinasalud3755 5 жыл бұрын

I love the way you discuss.. by saying "We" hehe

@KYADA1NONLY 6 жыл бұрын

Amazing!

@okbazahra6298 8 жыл бұрын

You are the best , thank u so much , your videos are so so helpful :)

@felixg4785 8 жыл бұрын

+okba zahra hi:)

@okbazahra6298 8 жыл бұрын

+Felipee Rincon hello :)

@mahya8450 Жыл бұрын

It can Not be better, thanks a lot ❤

@jyotijadhav9830 2 жыл бұрын

Super explanation sir

@biochemical846 7 жыл бұрын

i have a presentation on isoelectric focusing on tuesday. i face problems in some points i cannot understand immobilized ampholytes, 2D electrophoresis is type of isoelectric focusing capillary method and SDS are also part of it. how to load sample in gel can you explain me

@marionmutundu6086 5 жыл бұрын

very helpful... Thankyou

@veliborcabarkapa4271 5 жыл бұрын

excellent

@anne8nOtrn 7 жыл бұрын

thank you so muchhh!! :D

@purvanshivakil2531 7 жыл бұрын

Thank you !!

@milicialina3193 3 жыл бұрын

Are proteins taken out of the pH gradient gel to apply SDS or is this done inside the gel?

@user-Kufamed_student Жыл бұрын

Great✨

@kimbokjoo6817 2 жыл бұрын

Respect ✊🏼

@TwiSTeDBeAnS 6 жыл бұрын

It's a technique of separation and visualization, but not used as a method of purification, as purification implies that the protein is in it's native conformation. SDS denatures all of the proteins present so that they could not retain any activity and lose their binding properties.

@superoxidedismutase5757 6 жыл бұрын

wouldn't the SDS apply a strong negative charge on all the proteins and affect horizontal motion towards the negative end in the Isoelectric focusing component? Assuming both SDS-Page and Isoelectric focusing is occurring together.

@gershonanim6072 6 жыл бұрын

They r done seperately

@bhawnakhanna8884 5 жыл бұрын

Nope... they aren't been done together...first you do the isoelectric focusing and then SDS page

@zazaza8917 8 жыл бұрын

Yo, thamk you very much mister

@user-kr7ww2gb3f 7 жыл бұрын

Question (might be stupid but ok xD) : with the 2D gel electrophorisis can you find out how many polypeptides,from each protein, there are at your first solution? No right? You just separate them based on these two different properties

@aritradas4558 4 жыл бұрын

But how do we separate those proteins which may have the possibility of having the same PI value as well as the same molecular mass. I.e they can't be distinguished by 2d technique?? Please advise.

@aravindvenkateswaran5294 2 жыл бұрын

This is to check stream purity. For separation, I would recommend using them in chromatography and looking at affinity chromatography in particular.

@marianavalenzuela2986 Жыл бұрын

Is this Native PAGE electrophoresis?

@tenzlha 6 жыл бұрын

can you reverse the steps and perform mass separation first and PI separation second. Why or why not?

@thebro4860 4 жыл бұрын

You can´t IP separation must be done first, because it separes based on the IP so you want the protein to be on its natural form and with it´s natural charge (+ or-), once you do SDS you denaturate proteins wich makes them to lose their dimensional conformation, they are long chains and SDS wich is negative binds to them, giving all proteins a negative charge and thats why now you can separate them ut of their size instead due att all of them have the same charge, wich is negative. So IP first then SDS always.

@amanda_dela 9 ай бұрын

you saved me from my supervisor !

@muhammadahmad-ft7fv 7 жыл бұрын

nice done (Y)

@lavendar1358 7 жыл бұрын

A youtube science teacher without an asian accent with a clear and concise explanation? I must be dreaming..

@lukestephenson1168 7 жыл бұрын

He realised there was a market and he is taking full advantage of it :D

@Aldream 4 жыл бұрын

i still dont get it

@prasannaarun03 6 жыл бұрын

sir plssss continue ur lectures on biochemistry plsss...plssss...plsss.........we r waiting........................

@anythinggoes4588 7 жыл бұрын

Create your own religion! This got to be scientifically religious the way you explain so clearly. Thanks

@kooroshesbati8761 2 жыл бұрын

I wonder how you denature proteins after isoelectric focusing to work in SDS-page, if you take gel from first step the proteins are in their isoelectric point and wont move.