The FLEx vector or "flip excision" switch uses the Cre Lox system to control gene expression. In this video animation, we'll review the basics of the Cre Lox system and introduce how the FLEx vector inducible gene expression system works so you can apply it in your own gene manipulation project.
In the Cre Lox system, a sequence of DNA can be excised, inverted, or translocated when it is flanked by a pair of loxP sites while in the presence of Cre recombinase. The orientation of the pair of loxP sites will determine the outcome of the gene recombination.
A FLEx switch has the gene or sequence of interest flanked by two different pairs of lox sites pointed in opposing orientations. When Cre is present, the sequence of interest will be inverted (using one pair of lox sites), with the intervening sequence (and the other pair of lox sites) excised irreversibly to prevent further Cre Lox recombination from occurring. This enables researchers to irreversibly turn gene expression ON or OFF when Cre is present.
A system where the gene of interest is in the inverted orientation (with gene expression initially OFF) enables addition of Cre to reorient the gene into it's correct orientation, turning expression ON. Such a system is called a Cre-ON system or DIO switch (Double-floxed Inverse Orientation). A system where the gene of interest is in the correct orientation (with gene expression initially ON) enables addition of Cre to reorient the gene into it's inverted orientation, turning a gene OFF. Such a system is called a Cre-OFF system or a DO switch (Double-floxed Orientation).
This video will also demonstrate how the Cre Lox FLEx vector switch can be used for tissue-specific gene expression, inducible gene expression, controlling multiple genes simultaneously and performing gene knock-ins.
The Cre Lox FLEx vector is a powerful tool for genetic manipulation - especially in vivo! Let us know if you have any questions by posting a comment below. Enjoy!
➜ For more information on the Cre-Lox System and the FLEx vector switch, visit our knowledge base: info.abmgood.com/cre-lox-reco...
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