Cre lox system | Conditional gene knockout using the Cre Lox system

Cre lox system | Conditional gene knockout using the Cre Lox system | site specific recombination

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This video talks about Cre lox system | Conditional gene knockout using the Cre Lox system | site-specific recombination.
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Пікірлер: 59

@IlyaKuzovkin Жыл бұрын

Thank you for making this video! It is by far the best one I have found on the topic of what Cre Lox system is and how it works.

@animatedbiologywitharpan Жыл бұрын

please share my channel link with your friends

@priyankanehrwal1935 9 ай бұрын

I was very confused about using TAM for activating cre. You made it very clear. Thanks a lot.

@animatedbiologywitharpan 9 ай бұрын

youtube.com/@letscrackbioexams94?si=ZWdPXv2NSpZeDHZu please subscribe to my second channel 🙏

@gabrielamaral3167 6 ай бұрын

Thank you my friend, great explanation!!

@marcom0196 Жыл бұрын

Incredible video !!! Thank u very much for this clear explanation !! ❤

@animatedbiologywitharpan Жыл бұрын

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

@puekai Жыл бұрын

Why is this technique not given a Nobel yet?

@animatedbiologywitharpan Жыл бұрын

No idea

@elsieureta2719 9 ай бұрын

Thank you for making this very simple! Very helpful!

@animatedbiologywitharpan 9 ай бұрын

please share my channel link with your friends and help me to reach big audiance

@irisfan5386 5 ай бұрын

The best explained of the system I have heard, thank you

@animatedbiologywitharpan 5 ай бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

@semb12 7 ай бұрын

Thanks for this video. Best explaination I have found.

@animatedbiologywitharpan 7 ай бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

@nishat_zaman Жыл бұрын

Amazing video!! I have a question, so in the LoxP mouse, all the tissues will have the gene you want to delete flanked by LoxP sites. And only in tissues where Cre recombinase is active will you have the gene deletion occurring. Do the LoxP sites around the gene interfere with the correct transcription/translation of the gene in tissues where Cre recombinase is not expressed? I am wondering whether the LoxP sites could interfere with the translation of the gene.

@animatedbiologywitharpan Жыл бұрын

Good question, in most of the cases it does not interfere. The loxP sites are small.generally they flank exon and not in orf.

@user-gf3bz7xx2f 9 ай бұрын

Super well-explained video! I hope you are my professor teaching me!

@animatedbiologywitharpan 9 ай бұрын

Please support me by subscribing my second channel youtube.com/@letscrackbioexams94?si=l2Di6Uc4hCYYa3ve

@puekai Жыл бұрын

The explanation is quite clear

@animatedbiologywitharpan Жыл бұрын

@yuribanicol9490 10 ай бұрын

hola, podrías ayudarme, como resolverias este problema: “Usted generó un ratón KO para el gen XYZ. Luego de la obtención de las quimeras usted obtuvo ratones XYZ+/-, los ratones XYZ+/- son viables. Al criarlos se obtuvieron crias con la siguiente frecuencia: 50% XYZ+/+, 25% XYZ+/- y 25% XYZ-/-, pero los ratones KO mueren a los pocos días de nacer. Usted decidió hacer un knock in para el gen XYZ, con el fin de verificar los tejidos en los cuales se expresa, encontrando que se expresa en el hígado, páncreas y riñón. ¿Cuáles son los pasos necesarios para obtener un ratón donde el gen XYZ solo se elimine del páncreas? Esquematizando los vectores que se utilizarán, indicando todos los elementos importantes para la transgenesis.”

@nilaratnadas5246 9 ай бұрын

Thanks for explaining the application. 🙏🙏

@animatedbiologywitharpan 9 ай бұрын

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@kngoud4647 Жыл бұрын

Wow , one of the best video on cre lox p . Thank you so much ... I understood 😊😊 #from UoH

@animatedbiologywitharpan Жыл бұрын

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@PinkuHalderVlog 24 күн бұрын

very nice explanation

@animatedbiologywitharpan 24 күн бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

@barbaramoreno329 Жыл бұрын

In KPPC models what does it mean when they say there's Kras recombination or P53 recombination?

@animatedbiologywitharpan Жыл бұрын

I am not sure about this

@ibrahimhassoun2595 Жыл бұрын

Thank you man!

@animatedbiologywitharpan Жыл бұрын

Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.

@shruthi8974 2 ай бұрын

clear explanation thank you

@animatedbiologywitharpan 2 ай бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

@kriteebiswas488 7 ай бұрын

Hi, can the cre recombinase travel to other tissues (even though it has a tissue-specific promoter) and knock out the GOI e.g. the cre is brain-specific but can it travel to the liver and knockout loxp flanked gene of interest? And is the loxp flanking also tissue-specific?

@animatedbiologywitharpan 7 ай бұрын

No it can't

@IlyaKuzovkin Жыл бұрын

Thanks!

@animatedbiologywitharpan Жыл бұрын

Recived with thanks. Please suggest topics on which you want to see videos

@user-ww5ii7nx6n Жыл бұрын

Thank you for being so clear in your explanation, super helpful video. I have a question; how can we use the CreloxP system to restrict a particular gene knockout to a certain stage of development (i.e. adolescence)?

@animatedbiologywitharpan Жыл бұрын

You need to use the cre ER system . Tamoxifen inducible Cre-estrogen receptor (ER) fusion protein. For this, Cre is fused with the ligand-binding domain of a mutated ligand-binding domain of ER, which does not bind to estrogen but binds to tamoxifen with high affinity. Cre-ER proteins are sequestered in the cytoplasm via association with the HSP90 chaperone. Upon addition of tamoxifen, tamoxifen-bound Cre-ER dissociates from HSP90, translocates into the nucleus, and carries out site-specific recombination between flanking

@user-ww5ii7nx6n Жыл бұрын

@@animatedbiologywitharpan Thank you for your help!

@animatedbiologywitharpan Жыл бұрын

Please share my channel link with your friends and help me to reach big audiance

@anshuu5339 10 ай бұрын

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@animatedbiologywitharpan 10 ай бұрын

I have no clue

@tushyamehta Жыл бұрын

Can you explain how AAV might be used with CRE-loxP sites

@animatedbiologywitharpan Жыл бұрын

I will try to make a video on that

@Lulla3sl 8 ай бұрын

thank you! better than me prof definitely lol

@animatedbiologywitharpan 8 ай бұрын

Please support out channel using super thanks. Your 1$ contribution is our motivation

@animatedbiologywitharpan 8 ай бұрын

Please share my channel link with your friends and help me to reach big audiance

@berlyglobe7 8 ай бұрын

Can you also discuss how does Off-target recombination can occur with Cre? I think its also called Cre leakiness... I'm still confused

@animatedbiologywitharpan 8 ай бұрын

OK I will make a video on that

@totohotmailcomable 8 ай бұрын

In order to study Dopaminergic Neurons morphology, why not adding a GFP reporter gene directly after the specific promoter ? Rather than Cre Gene and a Flux stop GFP gene.

@animatedbiologywitharpan 8 ай бұрын

It's also possible, in that case that mouse line has to be generated

@pranabkarmakar7775 7 ай бұрын

Please make it a detailed version of this video! You have a great concept on these topic but when you are teaching you are jumping into related topics very often thats makes understanding quite difficult! Please make a detailed video! I request you! Thank you sir!

@animatedbiologywitharpan 7 ай бұрын

Thanks for the detailed feedback