Coronavirus Test: Real time RT-PCR

Coronavirus Test: Real time RT-PCR - Animation video

Рет қаралды 5,518,062

4 жыл бұрын

I make animations in biology with PowerPoint, this animation video is about the standard coronavirus test, real time RT-PCR method, which is a laboratory technique combining reverse transcription of RNA into complementary DNA, and amplification of specific DNA targets using polymerase chain reaction (PCR).
Coronavirus Antibody Test - Animation video :
• Coronavirus Antibody T...
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@biologywithanimations 4 жыл бұрын

Certain terms such as genome, lysis, and polymerase are mispronounced, because the voiceover is a synthesized audio.

@shraddhaeknathkhaire9787 4 жыл бұрын

Very nice.. please explain Antigen Test of Corona

@puspitasaha3412 4 жыл бұрын

What do E and N gene code for in coronavirus?

@MattUnboxTV 4 жыл бұрын

@@shraddhaeknathkhaire9787 you mean antibody test? Its so easy if you have antibodies in your blood (serum) it will travel through the cellulose membrane of the test kit which there are stationary anti immunoglobulins made from goat blood that will attach to your antibodies (if you have them) which will result in a colored line in the test kit. So how colored line formed is like this: antibodies to corona+anti-immunglobulins with colloidal gold->travels through test membrane->anti-Ig in test region attaches to that complex=colored line. Its called sandwich immunochromatography.

@ashaqhussain4558 3 жыл бұрын

Very nice info, i have confusion on RNA extraction, thnx

@CarolinaGirl6919 3 жыл бұрын

Thought that might be why!

@prakaash3712 3 жыл бұрын

As a Doctor , Im saying you have explained something that even my Professor cannot do ... Hats off buddy

@huhshru6519 3 жыл бұрын

Hello prakash sir where go u song ur practice I am a mbbs student

@juliablaszczyk8036 3 жыл бұрын

Greetings for that Professor! 😅

@reginagilmore3881 2 жыл бұрын

How are you doi a true test

@user-ov7lh6kz8u 2 жыл бұрын

In other words, the professor AND the doctor are both clueless about what's really on the tip.

@BushCampingTools 4 жыл бұрын

As a biologist,I have to say this is a fantastic animation for RT PCR! Thanks for posting!

@BushCampingTools 4 жыл бұрын

@@sivabalankesavan4339 Interesting, I'm going to look into this.

@kennethfernandez2645 4 жыл бұрын

YES!!

@devinify 4 жыл бұрын

this is like a whole semester lecture and lab condensed in a youtube video.

@BushCampingTools 4 жыл бұрын

@@devinify The power of good and accurate animations in modern teaching!

@sooryakeerthips8919 3 жыл бұрын

As a biology student i felt same as you!!

@ummmmmmmmm200 3 жыл бұрын

that's crazy. i didn't understand a thing. i truly admire those smart people that are able to come up with something so complicated. you guys are the coolest.

@carrybhai7312 3 жыл бұрын

Im in 10+2 studying for medical and they taught us about this basic idea test of pcr test. They taught us only three steps 1.denaturation. 2.annealing. 3 extension

@zahin007 3 жыл бұрын

@@carrybhai7312 yes.

@nidhithakur5479 3 жыл бұрын

Yessa❤️

@cristinanavarrete200 3 жыл бұрын

Not every people working on these things is super duper smart. Running the test doesn't necessarily mean you understand what you're doing. It can be done mechanically by anyone who can memorize the steps and have basic knowledge of it. Science is a collective effort of many researchers throughout our history. That's why on one side we have exceptional scientists who are more famous for their amazing contributions and on the other side we have regular scientists who aren't as geniuses but they also contribute to the human knowledge working with peers as teams.

@dalgug 3 жыл бұрын

I understand completely as I studied this science for a long time

@ruipedro9736 4 жыл бұрын

I’m a biologist and currently taking a master‘s degree in Biotechology and this video is very well done and accurate!

@uzzifennelli598 4 жыл бұрын

I just finished mine.

@ruipedro9736 4 жыл бұрын

@@uzzifennelli598 congrats!

@stephenpowstinger733 4 жыл бұрын

Funny reading replies to commenters who have disappeared.

@shreya446 3 жыл бұрын

i'm currently doing my masters and these videos are great if you need visual aid for learning. i haven't found too many good bt animation channels on yt yet but i'm on it...

@shreyadasmahapatra5020 3 жыл бұрын

How much time does it take to complete the whole process?

@schnurbs476 4 жыл бұрын

As a freshman science major I literally started freaking out when watching this because we had a lab in biology where we did this with fish dna. Its so cool to see stuff that I did in class being done in real life.

@protoolsfanatic7276 4 жыл бұрын

my eyes just glazed over after 10 seconds. it is like a whole other language. We need more people like you and for the future of medicine..good luck.

@aakashmitra1114 4 жыл бұрын

Schnurbs while you become an old man science person, it is really cool, believe me..

@kden5204 4 жыл бұрын

ME TOO!

@BeckyMatthew32572 3 жыл бұрын

I didnt do anything that cool in school, although I was a a community college so...

@quisperojas8888 2 жыл бұрын

Goood!!!

@user-pr8nq1zl5z 3 жыл бұрын

After watching this my sympathy for biology student is dramatically increased!

@danishanwar5306 3 жыл бұрын

Me a bio student

@jordanalwan82 3 жыл бұрын

The worst part is that this isn’t nearly as detailed as the exams lol

@rutviktaviyad5677 3 жыл бұрын

We are biology engineers

@galaxygirl7 3 жыл бұрын

Yeah I feel bad for the labs right now...🥺 so much workload !

@prashanttewari96 3 жыл бұрын

Thanks

@mohdtariqueanwar8146 3 жыл бұрын

I m a happy engineer now.... Respect 🙏

@thabisor1880 4 жыл бұрын

This feels like one of those exam questions where the solution is sequential, 1 wrong step and the whole thing is bust.

@purami14 3 жыл бұрын

Like Calculus equations.

@alfonsosoriano171 4 жыл бұрын

after 1 minute, i was just pretending to understand everything.

@rdyjur 4 жыл бұрын

I just removed my headphones imma just watch the pictures

@shoayebreza 4 жыл бұрын

Same here.

@naufikuk2179 4 жыл бұрын

You just pointed me out.

@deeptotop 3 жыл бұрын

I just thinking business is best instead of science 😆

@danarba3511 3 жыл бұрын

How long did you need?! For me 10 seconds was enough :))))))

@yuvraj6556 3 жыл бұрын

Whoh! I never thought class 12 biology chapter 6 would have soo much importance in real life 😆😆. I legit understood everything.

@rahulchougule9277 3 жыл бұрын

It is not class 12 biology. It is not so simple

@monarkbose7897 3 жыл бұрын

Ya true👍👍

@AbhishekSNair-sh4zw 3 жыл бұрын

@@rahulchougule9277 it is class 12 biology. The whole thing is there in ncert

@chocoballs530 4 жыл бұрын

Me: curious how they test covid something. After watching the video. Me: staying at home is simple.

@EnderGirle 4 жыл бұрын

Lol I agree! Though, working with these type of techniques is not necessarily difficult. It´s more time consuming and relatively expensive.

@nosi4524 4 жыл бұрын

shree umate unfortunately, not everyone has the privilege to stay home because of work and some countries like here in Sweden, there is no quarantining

@RD-ij2sz 4 жыл бұрын

Best Choice . I too will follow that .👌

@Geetha.digitaldd 4 жыл бұрын

Offcourse same feeling

@houseenp 4 жыл бұрын

Exactly, but in this moment, some stupid people think that the virus is not real and it is a government plan for our manipulation... :/ Greetings from Mexico!

@-Reddy-qd8cn 4 жыл бұрын

As bioinformatics student I was really impressed by this video.Take bow for your efforts sir 👌👌👌👌

@shivram9760 4 жыл бұрын

Which college?

@shashankjagata2420 4 жыл бұрын

Lol now I understand the importance of those molecular biology lectures in med school !

@vishwasreddy1321 3 жыл бұрын

I like how you're trying be "cool" by using the word medschool....

@blessedslave 2 жыл бұрын

He might really be a doc

@swamiaman7708 3 жыл бұрын

How many people are understanding nothing? The one who is understanding everything is really great.

@oshorai3258 4 жыл бұрын

Even if you have to animation app, the time and effort for organising the material and learning about it is immense. This is gold for learners. Thank you

@Alina28357 3 жыл бұрын

The first video I found after a long search that actually explains how the detection is happening. Thank you!

@Jeleky 4 жыл бұрын

It’s really cool to see that what we did in bio lab last semester is being done in real world scenarios

@amelialu9875 3 жыл бұрын

As a biology PhD student, I actually didn’t not understand the inner structure of the filter membrane until watched this video! Well explained!

@GiripunjeBhoomika 3 жыл бұрын

For 1st 4 -5 min I was able to understand and regain my knowledge which I've studied in my 12th grade and later that everything just bounced over and messed up .. I truely appreciate your work your job 🙏🏼🙏🏼.

@chess1011 4 жыл бұрын

No kidding when the doctors say the test takes time!

@shaunbooval9223 3 жыл бұрын

It actually does not take that much time. Depending on what instrument you use you could get a pcr result in under 2 hours. Technology and Automation has come a long way over the last decade in PCR.

@johnnguyen4152 3 жыл бұрын

40 minutes nowadays, Doctors don’t do the test. Lab personnel does.

@Pragalatha_Narasimman 3 жыл бұрын

This would be the last time I looked at a video that explains Microbiology or Virology. I watched 6 mins, I came here for explanation of how the virus is Diagnosed, in the 6 mins I needed 100 further explanations.

@prbnrjl 2 жыл бұрын

Thanks to all the Scientists and Engineers who stayed in school and contributed to the development of such a cool technology.

@poonamkoirala4466 3 жыл бұрын

I m a microbiology student nd yesterday i read theory of pcr technique in my biochemistry book nd today youtube recommended this now i fully understood the topic. thnku so much it helped me understood practically for my exams🙏

@2626ization 3 жыл бұрын

I love biology to! Be a great microbiologist. 👍

@vigneshsekar5993 4 жыл бұрын

I studied Civil engineering and this video information is correct and accurate!

@bambontuwang8973 4 жыл бұрын

This is the most accurate reply ive read so far😂

@RD-ij2sz 4 жыл бұрын

Yes . I am a mechanical engineer and I too certify that it's correct . Anyway I continue to stay at home .....!!!

@KapilSharma-iv8fi 4 жыл бұрын

I am a practicing Chartered Account and I certify this video to be correct and accurate!

@lingling2823 3 жыл бұрын

Civil engineers learn this stuff???

@rambo6714 3 жыл бұрын

Lol good one mate 😂👍

@gugachad1900 3 жыл бұрын

I'm a Medical Technology student, and I can say that this video is simple but accurate. Also, great illustrations! It really allows me to fully grasp my learnings

@melonhuskx15 4 жыл бұрын

*Suddenly everyone in the comments has a degree in this field*

@2012Croatia 4 жыл бұрын

I do not agree.

@aminanazeer6864 4 жыл бұрын

ya man

@LongySchlongy 4 жыл бұрын

Maybe some. Not everyone

@RD-ij2sz 4 жыл бұрын

Including a few in civil and mechanical engineering.....🙂

@kden5204 4 жыл бұрын

I learned this in bio 189 my freshman year of college

@MultiAxian 3 жыл бұрын

I'm a medical laboratory technician and I have to say this video is really well done the detail is spot on.

@aijaznazir3104 3 жыл бұрын

I swear i didn't understand anything but enjoyed the video 😭🤣

@adamwishney5369 4 жыл бұрын

Literally the best and easy to understand video explanation of RT-PCR I have ever seen. Thanks a lot for making this!

@sengnachi2sangma 4 жыл бұрын

I think this is one of the best video , nicely explained in a simplified way of the entire procedure and process of PCR !!! Thumbs up to the creator

@ariellepatra2613 3 жыл бұрын

As a molecular tech, this video does a fabulous job of explaining how the PCR machine works, as well as how wash, elution, etc buffers work! This is awesome! :))

@dikshitapandey5878 3 жыл бұрын

I m in class 12th and have a chapter biotechnology principles and processes in which PCR is explained and I m really shocked how little we have learnt from our ncert bio book about this topic...😨😨

@adityasuryawanshi8527 3 жыл бұрын

Same here

@abhinavgoswami6006 3 жыл бұрын

Yess!!

@himanshugangwar1 3 жыл бұрын

I am also in 12 but i have Optional Subject Biotechnology and I read it.

@samriddhipathak421 3 жыл бұрын

Yeah.....it was just given PCR...and in three steps....it is done....but see here....it was like how little we know 🙄🙄...... But one more thing.... during watching of this video....it feels like our teacher is explaining molecular basis of inheritance Chapter 😂😂😂😂😂

@nonchalant4043 3 жыл бұрын

We cannot understand until we do undergraduate courses in bio. In 11th 12th is basically an introduction to collage courses. So wait

@vans4lyf2013 4 жыл бұрын

Amazing video! Fantastic explanation and fantastic animations. Really well done!

@UCS_BibaswanChakma 3 жыл бұрын

holy moly. I just understood the PCR concept which i struggled so much in biology and i never thought i could understand >_

@kapilupadhyaya5663 Жыл бұрын

Recently switched my field from chemistry to biochemistry and ran couple of PCR reactions for protein expression. I liked this video very much. Hats off!

@kvigneshraja 4 жыл бұрын

I have a Master's Degree in Management and I have to say this is absolutely accurate to point. With very little detail dispersion.

@surjeetsingh6321 4 жыл бұрын

Each and every word he spoke passed over my head.. 🤨🧐😶

@vikasgondaliya3958 4 жыл бұрын

So I'm sure u must not from science background.

@matias9760 3 жыл бұрын

Tomorrow I have a job interview, and this video helped me remember many aspects of PCR that I had forgotten, I am very grateful!

@samanthac323 3 жыл бұрын

how was the interview

@FransceneJK98 3 жыл бұрын

What job?

@HimanshuSharma-xn6uc 3 жыл бұрын

All the best bud

@catwithhands 2 жыл бұрын

Did you get the job?

@subhadeepmitra5492 4 жыл бұрын

A very comprehensive and lucid explanation of RT PCR. Great animations,great job!!

@mananshah3197 4 жыл бұрын

As a biologist and epidemic expert, I must say that the animation is very simplified and wonderful.

@michellekochifas3157 4 жыл бұрын

Best video!! I dont understand how to do a pcr, but now I watch this video and I understand all. Just this year I had to lear how to do a PCR

@WinxClubRoxyFairy 3 жыл бұрын

One of the best RT-PCR videos so far on the Internet!

@salkhan20 3 жыл бұрын

This gain huge respect to people who research and test such complicated stuff.

@darylsarandi9028 4 жыл бұрын

I just felt the need to go to CR after watching this PCR.

@najibali8196 4 жыл бұрын

I just found this channel on KZfaq. And I am really impressed with the video. This is one of the best PCR videos I've ever watched. The explanation is concise and simple. The voice is quite clear, so as an Indonesian, which English isn't the mother's language, I don't have any difficulties to listen and understand your explanation. Thank you for your effort to make this video and share to us. Sorry for the bad grammar ;)

@faresalhawaj9936 4 жыл бұрын

Best video to explain RT-PCR protocol steps so far. Thank you!

@sanchari.c 4 жыл бұрын

We studied this in our final year of Bachelor's degree and were about to give the final exams before we went into complete lockdown. This is SO exciting to watch. I cannot get over how simple yet elegant these techniques are. Thank you so much for this!

@daamu48 4 жыл бұрын

An excellent video. Within a very short time it explains everything .

@pritambanerjee1377 4 жыл бұрын

Beautiful Animation with great explanation. Thank you.

@sapphire.k7414 4 жыл бұрын

you make rt-PCR explanation really simple XD. if only my professor explains like this, I probably will ace my test in MCB haha

@lluminascent 3 жыл бұрын

This is the best explanation of PCR ever. Currently writing up my PhD thesis and I'm doing a section on comparison between PCR and NanoString techonology. So this was just what i needed!

@Vijay-oz3sr 3 жыл бұрын

Found this to be a complex procedure, hats off to the number of samples being taken on a daily basis

@dawnhoran3353 4 жыл бұрын

Brilliant step by step guidance and explanation. I love it!

@JN-hu2do 3 жыл бұрын

Fantastic animation. Helped refresh my memory before I go for my COVID-19 laboratory technician interview tomorrow!

@piw5142 3 жыл бұрын

This was really helpful thank you. We were given an assignment to find out everything we can about covid 19. From the pcr test to the how the vaccine is made and how it works. This was helpful

@brucevogelsberg3452 4 жыл бұрын

Taking an intro biochem course right now over the summer and just learned about PCR. This video was up next from a PCR animation recommended and I was curious. I got the PCR stuff and some of the centrifuge stuff (from what I've done in bioengineering labs) and I'm honestly very surprised with how much I understood.

@javiercastillo5390 4 жыл бұрын

Amazing video, thanks so much...

@MukeshKumar-xl8tp 3 жыл бұрын

thanks for this initiative of edifying biology with animations.hats off!

@pascalokoye2451 4 жыл бұрын

This is amazing. The animations explain qRT-PCR in a short and coherent manner. Thank you so much.

@sweetspirit1419 4 жыл бұрын

Best video I have found so far! Thank you!

@Powerofou 4 жыл бұрын

Very good explanation! With easy animations, it is understandable.

@viralvideos1659 3 жыл бұрын

After trying to understand the process i am blank... I just want to salute all the person who invented this complex process🤗

@aarthipanth1328 3 жыл бұрын

As a Biochemistry student,we have learnt this as part of lab practical.. Bio subject is very interesting

@juantovar1147 4 жыл бұрын

this guy is a genius

@viobejan2377 4 жыл бұрын

amazing ! thank you!

@helljason 2 жыл бұрын

Such a long process, hats off to these people doing all these tests.

@michaelyblam 2 жыл бұрын

As a layman, I am incapable to decipher most of the stuffs explained but I trust that this is a video well made as I can see those subject related viewers praising how good this video is.

@debasishkalita1529 4 жыл бұрын

I was searching for PCR related videos and found this in recommendation, nice keep it up

@vaishnavibhardwaj9970 4 жыл бұрын

m a junior microbiologist and'll complete my graduation this year this video is really very appreciating dude👌

@shailendrakumar-vv8ey 4 жыл бұрын

vaishnavi bhardwaj Good job

@Canadainfonews 3 жыл бұрын

As a pathology officer it is very easy way to explain all about the PCR step wise . The animation accurately shows all steps involved and explained in very simple way . The RT-PCR is simple method , many non technical people may find difficulties but we have been doing this for other molecular tests before.

@javieraugustorodriguezbrav7597 4 жыл бұрын

Excelente animación dem PCR-RT. Saludos desde Lima, Perú.

@soniapitta1586 4 жыл бұрын

Nice to know what is going on with Real time PCR

@gasimalamin2723 3 жыл бұрын

As a doctor I would say this is an amazing video to understand Covid-19 Test

@anissemenyih8924 3 жыл бұрын

Its not Only for Covid 19 Testing.

@justsomedudewithmustache7462 3 жыл бұрын

As a biotechnologist, I'm saying that you have explained it brilliantly.

@popping9774 3 жыл бұрын

Being a BTech bioengineering student we missed such practicals in lab due to covid, thank you for explaining it and animation was really great.

@m.montufar4880 4 жыл бұрын

Thanks for this!!!

@syahiran2188 4 жыл бұрын

Good explanation, now i know how rt pcr work

@sarrusophone 2 жыл бұрын

best speech synthesis I’ve heard in my life to date

@harshvardhancharan4994 3 жыл бұрын

It feels awesome when i understood what i do in laboratory.. while dealing with the machines which do this whole process.. proud to be a biotechnologist!!

@AnandAryan17 4 жыл бұрын

As a dumbest student, I just felt like I was in my school lecture of chemistry.

@priyansus1519 3 жыл бұрын

It's Bio technology bro

@framedheart 3 жыл бұрын

This perfectly deviates us from asking about false positives

@hugomixmax1 3 жыл бұрын

This is how they "soft" the test, by making it pretty, its a amplification and copy machine, just that

@daleyakin2053 2 жыл бұрын

I'm a business guy who has to create slides explaining this stuff to investors, and this good work is invaluable.

@the_hiroman 3 жыл бұрын

Mad respect to the biologists and medicine science researchers. I have a Masters Degree and I find this very complex and elegant.

@HereToday831 3 жыл бұрын

I love how biotechnology works.

@durgakadu5085 4 жыл бұрын

Wow such a great explanation. As I am a biotech student feels like can do it in lab with proper precaution

@smartcookie823 4 жыл бұрын

same, I do biochem

@trinitaccad3066 4 жыл бұрын

Precisely!

@abdulsoabirshaani3629 4 жыл бұрын

A biotech student??

@trinitaccad3066 4 жыл бұрын

@@abdulsoabirshaani3629 students who are majoring in Biotechnology

@mercycherisa.5607 4 жыл бұрын

I studied Biochemistry, so its quite interesting to see all the book work playing out.

@Arai_Somnia 3 жыл бұрын

Thank you very much for posting this video! I have a new job, where I have to do exactly this! Since I was a laboratory technician in the agrobiology field I haven't had much experience in PCR.

@jamhombre 4 жыл бұрын

This makes me feel like when your're in class and don't understand a word the teacher is saying, but then you pretend to get it and then hope that when you get home and read upon the topic that you'll get it then and won't feel like a total dumb a..s.. LOL

@MaximumEfficiency 3 жыл бұрын

@mackisbrocklesnar 3 жыл бұрын

This is really nice explanation of RT-PCR. Well done for this. Only thing I would have liked is if you would explained thresholds of 24 and 35 CT that are important in Covid-19 testing.

@bigpardner 2 жыл бұрын

Why were they using CT of 40 and above?

@zanitaglendaplaga7740 4 жыл бұрын

Thanks, this gives me an idea how the test is performed... Well explained :)

@shikhasharma0038 3 жыл бұрын

As a microbiologist... i can say this was amazing clarification of RT-PCR by animation... it was amazing way to learning too gud information in a vry smooth manner with a particulate diagram nd techniques... thnkuu very much.. m your new subscriber... 🎉🎉👍

@weathergenerator 4 жыл бұрын

A super informative video that will make even average minds conversant. Kindly do more videos on different issues.

@martianalien6696 4 жыл бұрын

Never expected that complexity 😂

@kimjoshuatacsagon1564 3 жыл бұрын

Right, but this process is happening very fast the only problem is the PCR amplification that takes more time than other processes

@nishyu9101 3 жыл бұрын

Woah! i don't think i'm ever going to complain about the report of the test being late!

@farykhan9569 4 жыл бұрын

This video was amazing so accurately they explain rtPCR method...👍

@devonvancura81 4 жыл бұрын

2:12 for an unbalanced centrifuge

@rodneytill741 3 жыл бұрын

I was thinking the same thing. Don’t put that tube in there alone!

@Ronaldino150 3 жыл бұрын

hahaha literally paused the video to see if I could find anybody who spotted that as well

@shreya446 3 жыл бұрын

oh i'm sure they'll have enough samples now, what will all those cases...

@131krishna 4 жыл бұрын

It's so nice and informative. Thank you for the detailed information in this video..

@4NasirAlDeen 3 жыл бұрын

Well done, thank you for this video that will allow others not in the laboratory profession to get an understanding of how COVID-19 is tested in a general sense.

@manish.r123 3 жыл бұрын

I thank to biologists/technicians doing millions of this cumbersome test everyday. These tests are hope for mankind. Thank you also for this complex informative animation and explanation.