cDNA Libraries and Expression Libraries | MIT 7.01SC Fundamentals of Biology

Рет қаралды 109,289

12 жыл бұрын

cDNA Libraries and Expression Libraries
Instructor: Eric Lander
View the complete course: ocw.mit.edu/7-01SCF11
License: Creative Commons BY-NC-SA
More information at ocw.mit.edu/terms
More courses at ocw.mit.edu

Пікірлер: 60

@user-xs5vg9yx3u 4 жыл бұрын

When that student said “Human cell”, i was like, nah, must be bacterial or yeast cell, then out of my expectation, the teacher went “That’s interesting, let’s put it as an option”. That is the moment i saw the light of science, education and my ignorance... Great lecture

@goncalocandeias3309 9 ай бұрын

and the difference between a good and bad professor

@MA2520 5 жыл бұрын

actually Eric Lander is so good that I watch these lectures for entertainment (and learning of course)

@martinahejnickova3090 8 жыл бұрын

Oh my god, I wish I had a teacher like this! Perfectly understandable AND funny? It doesnt feel like studying anymore :D

@godslove7301 7 жыл бұрын

Same. i wish my lecture actually care if student understand the material or not. all the care about if getting to the of their prepared slides that's all

@nohemie4057 6 жыл бұрын

That's what I call teaching! I wish I had more incredible professors like this ! Thanks a lot !

@richdog490 8 жыл бұрын

He does a great job of making it interesting

@daniellebelize1862 8 жыл бұрын

Well done. I almost got teary-eyed. I miss college!

@samaben4812 4 жыл бұрын

Incredible teacher, MIT students have lot of chance

@soniaorjuela8050 10 жыл бұрын

Amazing! Wish that was my professor!!!

@Fatheha95 9 жыл бұрын

I have to write an essay on this, and was unsure of the steps. Thanks for the clarification, i wish my lecturers were this good at explaining!

@kritikar2939 10 жыл бұрын

I agree with u guys.. I like the way he taught that, he made it look so simple. I wish to learn more from him...

@KaranKapuria 9 жыл бұрын

Wonderful Lesson a midnight before my exams

@victorleon3925 10 жыл бұрын

The best teacher ever...

@prashantkumarparmanu 5 жыл бұрын

he is the ultimate teacher of Biology.

@Elif-eo5dn 2 жыл бұрын

The lecture is a legend.

@mohammedkulaibi1351 11 жыл бұрын

what is great professor, i wish to learn from one like this

@MattPresent 9 жыл бұрын

This was wonderful! Thanks very much. Extremely helpful and engaging.

@nasserismail2438 9 жыл бұрын

This guy makes sense to me.What a wonderful lessons....Thanks% you are a mazing

@ThePhotogalleria 8 жыл бұрын

Wonderfully presented!

@petermurithi1670 9 жыл бұрын

This is my permanent teacher

@fairytaillover8 9 жыл бұрын

eric lander saved my sorry self today after i zoned out in class thank you based god eric lander

@hafizaamna3423 6 жыл бұрын

such a cool professor

@calebm9000 5 жыл бұрын

This guy is great. Pretty fast for my brain but his energy is infectious. Jealous of those students!

@songthanh896 Жыл бұрын

Really helpful, thanks so much

@marlenesoifer7219 3 жыл бұрын

I'm doing good with this time thanks to your help

@wenguo66 2 жыл бұрын

Really a great teacher!

@ramya.bm.krishna4257 5 жыл бұрын

Wow excellent lecture😊

@johnvandenberg8883 7 жыл бұрын

Great teacher!

@mahamkhan9002 2 жыл бұрын

U r amazing teacher 😃

@kundhan829 4 жыл бұрын

its in the catalogue. love from india

@fvveb2141 6 жыл бұрын

perfect lecture

@TlhousemusiclT 10 жыл бұрын

Amazing Prof.!!!!

@silviapabon1282 3 жыл бұрын

these Master is awesome... hahah I am working in 4 sec the daily question .great

@OutstandinMediocracy 11 жыл бұрын

sounds like we have the same professor :P, except mines with a french accent

@leonardosoto4603 4 жыл бұрын

I wish the opportunity to one day study at MIT

@moussaouiahmed 9 жыл бұрын

oui exacte

@150iSolony 12 жыл бұрын

I would like to have such a teacher. Chciałbym mieć takiego nauczyciela.

@yasiraa7919 6 жыл бұрын

He's preparing them to be inventors.

@edok2306 6 жыл бұрын

sun everything comes from it, photosynthesis, next next. and d 4 terrestrial plan will take when suns color is our red.

@Zk_Vist 9 жыл бұрын

@ 7:14 - 7:32 One question, I suppose we're growing Beta Globin cells for the purpose of using it? If the antibody sticks on the Beta Globin cells, these cells become un-usable? Anyone who knows the answer, pls share your knowledge.

@jackc7069 9 жыл бұрын

I suppose we'd grow these cells in order to use the beta globin itself (as in the example of insulin at the end). In that case, attaching the fluorescent antibody to the cells would kill them in the process because, as he explained, you'd first have to destroy their membranes and spill out their content. Thus the cells die, but the beta globin is still usable.

@Zk_Vist 9 жыл бұрын

thanks a lot. now I have some questions. 1) how do you destroy their cell membrane without denaturing the beta Globin protein? 2) and how do you isolate the beta Globin protein from the mess? looking forward to your reply.

@jackc7069 9 жыл бұрын

Leon, Ye Lin Kaung 1) There are many ways to break down the cell's membrane (a process called "lysis"): repeated freezing and thawing, sonication, homogenization by high pressure, filtration, or permeabilization by organic solvents. The method of choice depends on how fragile the protein is and how sturdy the cells are.

@jackc7069 9 жыл бұрын

Leon, Ye Lin Kaung 2) This usually depends on the mass of the protein. Anything that weighs more than 10 kDa can simply be extracted and purified by a centrifuge (either zonal or differential). Beta globin is just under 16 kDa, so that should do the thing.

@jackc7069 9 жыл бұрын

Leon, Ye Lin Kaung Otherwise you have to rely on other techniques that target other characteristics of the protein: electrophoresis (separates in function of charge), chromatography (in function of mass, charge or strength of its bonds) or simply an enzyme that binds to certain proteins

@Devoidzylon 7 жыл бұрын

Learning in the United States seems so easy. In India, in the same amount of time, you need to process five times more data due to the competition and resulting higher floor of knowledge.

@macielrodriguez6697 6 жыл бұрын

How so? :O

@field-yetian6001 2 жыл бұрын

I dont get this. If you wanna clone a protein from human gemone, you simply design a specific pair of primers and amplify. Why bother fluorescent ab selection?

@ivienprinzefetalino3410 7 ай бұрын

In my understanding, flourescnt antibody will bind to the beta globin protein in the filter paper and this would tell us which cell or colony actually expressed the beta globin gene.

@malumaha5821 3 жыл бұрын

Pls upload elaborately about chromosome walking

@Bob-fj7lr 7 ай бұрын

bookmark 11-23-23

@TheLastSoviet22 11 жыл бұрын

Make a Protein! ;)

@chetan4851 10 жыл бұрын

flawless teaching... but dumb class nobody answers the questions

@jonjon11882 9 жыл бұрын

Right? This is MIT right? I go to UTEP (not even a tier 1 university) and I knew how to make this contruct right away and I don't even have my bachelors yet.

@christopherdonaghue2461 9 жыл бұрын

Jonathan vazquez This is 7.01 - an INTRODUCTORY biology course.