i have noticed in the early video, thank you for correction!

@@AriffMahtar Actually we did a silly mistake in the calculation. Please ingore it.

Isn't 56.1 the molecular weight for KOH.. And you used NaOH in the experiment

@@crayversace2804 AOAC method said to use 56.1 as the factor of this calculation for in both of the cases whether you use 0.1N KOH or 0.1N NaOH solution.

So what would the formula be if I used 0.1 KOH, 10 grams of oil, and had a titration volume of 0.76

Glad to know

Thanks.

Thanks. Free Fatty Acid calculation is given in the description section. Please take a look

My pleasure 😊

Thank you very much for your appreciations. We do not have video for ICP-OES. In future, we will try to make video on it. We will upload video on GC soon. Stay with us.

I am microbiology test only analysis,but your video will help to learn Crude protein analysis,crude fiber and crude fat test is very easy and result is very good

Thank you soo much for such appreciation. We uploaded standard preparation for AAS. We. Also uploaded one video for GC and several video for spectrophotometer analysis. In future we will upload for ICP-MS too. Stay with us. Soryy for late reply.

Very educating and simple too, but please how do I estimate the free fatty acid from the acid value dealing with Shea butter sample

Vipin Pathak, you are right. NOTE: There is a mistake in the formulae of acid value, mentioned in this video, and the formulae for FFA is not given in this video. Both corrected formulae & calculations of Acid value and FFA are given below: *Acid value (as oleic acid) = (56.1×V×N )/W So, Acid Value = 1.6 *Free fatty acids (as oleic acid) = (28.2×V×N )/W So, FFA = 0.805 percent by weight

@@MicroChemsExperiments hi may i know what is the 28.2??

@@dvnv6250 calculation factor

Right ho aap Aap kha se ho bhai

@@luckynamkeenfryums modinagar ghaziabad

Corrected Calculation is given in the description section of this video

Yes. Correct

No idea about your sample. You can try and let me know the results.

You are most welcome.

Appreciate 👍

Will try

Rahul Pawara, thank you so much. Be with us.

Please follow our video for the Determination of crude protein and crude fat. Search on our channel to find those videos. I think you will find them easily

No. That will be wrong

You are welcome

1. You can find the difference between normality and molary from google. 2. Purpose of stadardization is to know the exact concentration of any solution. 3. Normally ethanol is slightly acidic. So first titration of ethanol is done to make the ethanol neutral as if it does not interfare the acidity of our oil sample.

@@MicroChemsExperiments So for every trial of this procedure, do I need to standardize the solution every time?

Also, may I ask where you got the expected acid values from? I need a citation. Can you please also explain the formula in the calculation section? Thank you very much for your help. I appreciate it :)

@@mysticforest6345 No. But standardize when you will analyze your sample on a different day.

@@mysticforest6345 Search on Google and find the reference value from journals and publications. You can also find it from countrys standard of that sample.

Thank you so much. Stay with us

Glad you like it!

Take help from Google for detailed explanation

Correct

Boiling of oil with ethanol will not facilitate to dissolve the oil completely. So no worries about it. Perform the test according to our video. You will get the accurate result.

Thank you

Thank you

Right

But why ? Please give me the reason I think when you titrated with 0.1 NaOH you multiple by 40.001. so I said to my boss that our specification is wrong because we multiple by 56.1

@@user-if7df1rm2w if you follow AOAC method, it is written that you have to use factor 56.1 whether you use KOH or NaOH

For proper mixing of oil

Fats/oils don't dissolve fully in ethanol at room temperature but do dissolve at the boiling point of ethanol. For this test to be accurate, the oil needs to be fully dissolved in the ethanol, so the ethanol is heated to its boiling point to ensure the oil is all dissolved.

Most welcome 😊

You are welcome

Thank you so much. Be with us.

Good video btw 🙂🙂🙂😀

This video is also applicable for FFA test. Just FFA calculation is different. FFA calculation is given in the description section of this video.

No. Boiling is needed for melting, dissolving and mixing of oil with ethanol. Boiling does not change the acid value significantly

Search on google, find from journals, international specifications and standards (Indian standard)

Son of a Snitch, you have to carry out titration when the misture is hot.

@@MicroChemsExperiments noted. thank you🙏🏻

Son of a snitch means?

We use 50ml of neutralized ethanol to dissolve the oil sample because AOAC method said so

You do not need acetic acid in this experiment

Son of snitch, do titration while it is hot.

Keep the flask on hot plate while titration and use magnetic stirrer to agitate the solution.

Yes. Do it

​@@MicroChemsExperiments FFA content in oil only or also content in dried sesame seeds?

We didn't try this method you described. Please try our method described in our video which was made by following the AOAC strictly.

It is not the test method for insoluble matter in alcohol and water.

You are welcome

Yes. Standardization of NaOH is must. We didn't show the procedure of standardization in this video. We just mentioned that standardization is needed. We developed another video for the standardization of NaOH and already uploaded in this channel. Please find the video if needed. Thank you

@@MicroChemsExperiments ❤️

We never tried intentionally oxidation of oil. So we dont have any experience about it. But we think that the difference should be noticable in your experiment. Please conduct your experiment again following this video strictly. Increase the oxidation time of your sample. Dont use excess ethanol. Use 50ml nutralized ethanol for 10ml sample and heat the ethanol before mixing with sample. Dont keep any change of the method described in this video. We hope that you will find difference between two different conditions of oil.

@@MicroChemsExperiments Thank you for your quick reply and explicit instruction. I will be sure to re-evaluate my method and follow the instructions of the video more precisely, with the ratios you've outlined. Thank you very much again for your response and kind words. 👍

@@PsykkMusic you are most welcome dear. Waiting for your experiment results.

@@MicroChemsExperiments Sorry to bother you again, but I just had a few further questions questions that have arisen as a result of some continued investigation, if you are willing to answer them. Primarily, I noticed that no element of the formula used to calculate acid value actually accounts for the particular oil to ethanol ratio, but from my experiments, increasing the amount of oil in the solution lead to an increase in the amount of NaOH used? Fortunately, for my experiment I've found that a 1:5 ratio has yielded results that work well in the calculations thus far, but someone suggested that I should test this with different ratios to further confirm the acid value, but I noticed the formula won't actually account for the ratio. It also doesn't account for the total volume of the oil/ethanol solution. Is there a way to calculate Acid Value to account for changes ratio's and solution volume? If not, I understand. Also, for the calculation of the amount of Oleic Acid present in the sample, is the formula the same as for the calculation of the Acid Value, just replacing the Molar Mass of NaOH with the mass of Oleic Acid? If so, would you be able to give a brief explanation why? Furthermore, If you have the time, I was just wondering why the solution needs to be heated? Is it just to increase the solubility of the oil? Could the ethanol be preheated to 95ºC and then be mixed with the oil? When I heat my 60ml samples for 10 minutes on a hotplate at 150ºC, I still notice some oil not completely dissolved? Should I wait until the bubbling becomes more aggressive? I greatly appreciate your efforts thus far and hope not to disturb you too much further after these comments. I understand I ask a lot, and am grateful for your efforts. Thank you very much for your time and I look forward to your response.

maybe you forgot to add indicator before titration.

No problem. During heating, acidic properties comes with ethanol.

Acid value does not have any unit. It is a value without unit like pH.

This method is applicable for all kinds of oil sample.

@@MicroChemsExperiments thank you. About the standardization, how is it performed please? The steps weren't mentioned here.

Different oils have different FFA value. Search on google to get idea about the reference ffa value of an oil sample

Do as done in this video

g is not enough for the analysis. Do the experiment using 10g.

Irfan Ali, Thanks for your question. Yes adding Phenolphthalein twice is needed for this test. First time Phenolphthalein is added to carry out the neutralizing titration. Second time, it is added for the Acid Value determination titration. Both are needed because during boiling Phenolphthalein activity is lost in heat. So you have to add Phenolphthalein again before starting the Second & Final titration. We will try to upload a video for rancidity test very soon. Stay with us.

Some measures of free fatty acids use KOH as the standard while others use NaOH. To convert from KOH to NaOH, multiply by 40/56.11. Likewise, multiply by 56.11/40 for the opposite conversion. This ratio is just the molecular weight of NaOH to KOH.

To be clear, the AOAC method uses KOH. If you're titrating with KOH, just use the KOH MW (56.11) instead of 40, and you'll have the right acid value according to the AOAC method.

Yes, absolutely

Yes.

For melting, dissolving and mixing of oil with ethanol

Not available at online. You can purchase from us. Write us at mail.mic.chem@gmail.com to purchase

You need to heat just at the boiling point of the ethanol. May be at 80 degree Celsius.

@@MicroChemsExperiments thank you very much!!!

@@AshishKumar-rf5tv you are welcome Sir

Same procedure will be applicable for groundnut oil sample.

You can use 96% but not 70%

Can you explain why you perform this test

Please find the corrected calculations with unit from pinned comment. Thank you

Boiling is needed for melting, dissolving and mixing of oil with ethanol. Boil for 1 min

Sorry we are unable to explain for oil mixture.

AOAC method describe in this way of heating

You are welcome

You can measure the acid value of ghee by this same method.

No. You can't

You should take 10g sample

You have to use 56.1 for both of NaOH and KOH

It will be same for rice bran oil??

@@lalitverma5279 yes

Please read the description section

You can use general moisture analyzer

If you dont have any reference expected value then test your sample twice or thrice asuming high, medium & low acid value. After performing the three tests you will get an idea about the acid value of your sample.

Noted. We will develop video later

You should use ethanol

You are welcome

Yes right

Sorry. We don't provide any document.

Boil until the oil is dissolved in ethanol completely

The higher the acid value and free fatty acid content, the lower the quality of the oil

AOAC method said so

Every oil has the same range

Different oils have different ranges and you need to adjust your sample weight accordingly. I work at a bioethanol plant that produces distiller's corn oil, which has a very high free fatty acid content (about 15-25). For this, about 0.5 g is appropriate. There's a chart early in the video that gives the right sample weight for different expected FFA concentration ranges.

It depends on the temperature you applied. Time doesn't matter, you just need to boil. That's all.

It represent the quality of oil. For more, study with the resources of google

It gives you a measure of how much of the fatty acids in a sample of fat/oil are free fatty acids, as opposed to being bound to glycerol as triglycerides. This matters for several reasons depending on the application - for instance, high free fatty acids can help oils to go rancid faster, or for biodiesel free fatty acids will be turned into soap, which will interfere with the process by forming emulsions or (if it makes it into the final product) damaging engines and fuel filters.

It would help to add it to your flask. But ultimately it doesn't matter as long as it's totally dissolved in your ethanol before you do your titration.

Correct

Corrected formula is given in the description section.

Noted

Soon

Thank you.

Reference is already mentioned in the title. No pdf is available to share