Determination of Peroxide Value_A Complete Procedure (AOAC 965.33)

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Күн бұрын

The peroxide value is determined by measuring the amount of iodine which is formed by the reaction of peroxides (formed in fat or oil) with iodide ion. The base produced in this reaction is taken up by the excess of acetic acid present. The iodine liberated is titrated with sodium thiosulphate.
This video represents a complete procedure for determination of Acid value of oil samples. _________________________________________________________________________________________________
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Пікірлер: 338

@yogeshmandole5953 5 ай бұрын

Please keep going, this is really helpful

@maryamnisar9697 3 жыл бұрын

Very nice description!!! I have watched the whole video Also good presentation Keep it up!!!!!

@MicroChemsExperiments 3 жыл бұрын

Maryam Nisar, thank you very much. Many upcoming videos are waiting for you. Be with us.

@suhanamughal1103 2 жыл бұрын

This video was very clear and help full. Thank you for the effort👍🏻

@MicroChemsExperiments 2 жыл бұрын

You are welcome

@mariovendrell3563 3 жыл бұрын

I am doing a PhD in oil quality and I found this video very interesting. Thank you very much, you are great!

@MicroChemsExperiments 3 жыл бұрын

Glad it was helpful!

@user-jk6yb4wz3s 2 жыл бұрын

Hello.. Have your Facebook account available?

@shraddhapiligaonkar3639 Жыл бұрын

I want a procedure for preparation of 0.5 N alcoholic KOH

@chemsciences126 Жыл бұрын

kzfaq.info/get/bejne/n6d8jM-pqtOydY0.html

@chemsciences126 Жыл бұрын

Dear Mario Vendrell Your are doing good. It's my channel, hopefully, my videos will also helpfull to you. Thank u

@ehsanasghar7448 10 ай бұрын

thanks, it is very helpful.

@user-qk1in7lx5e 10 ай бұрын

thanks for the great video

@prathameshmhatre1211 Жыл бұрын

Thanks I have my practical exam and this helped a ton!

@MicroChemsExperiments Жыл бұрын

Great to hear!

@abianand9582 2 жыл бұрын

Super . Your effort is nice and best hard working . Thank you

@MicroChemsExperiments 2 жыл бұрын

Thank you

@meltunivers6475 3 жыл бұрын

thank you so much for this video

@MicroChemsExperiments 3 жыл бұрын

You are most welcome.

@Farmville91 3 жыл бұрын

Thank you!

@MicroChemsExperiments 3 жыл бұрын

It's our pleasure. Stay with us.

@AbhishekSharma-mo3nb 2 жыл бұрын

Keep it up Doing great 👍 And thanks for vedios

@MicroChemsExperiments 2 жыл бұрын

Thanks

@impostor4377 3 жыл бұрын

Thank you for sharing this information.

@MicroChemsExperiments 3 жыл бұрын

Norman Guanizo, you are welcome

@impostor4377 3 жыл бұрын

MicroChem's Experiments do you have details on how create citric acid sulotion for physical degumming. Let say i have 1L of coconut oil how much citric acid and water to mix to perform degumming.

@payrawsalh6485 3 жыл бұрын

thanks a million

@MicroChemsExperiments 3 жыл бұрын

You are most welcome. Stay with us.

@pranaysarkar1611 3 жыл бұрын

Lovely

@MicroChemsExperiments 3 жыл бұрын

Pranay Sarkar, Thank you so much. Be with us.

@user-xb2cq9kl3j Жыл бұрын

Good video guys keep growing

@MicroChemsExperiments Жыл бұрын

Thanks. Stay with us

@nasabanicholas8496 26 күн бұрын

Very helpful

@MicroChemsExperiments 24 күн бұрын

Thank you

@VivekKumar-eu3ty 2 ай бұрын

Thanks mam

@tsmtvipergaming6819 3 жыл бұрын

nice work

@MicroChemsExperiments 3 жыл бұрын

Rahul Pawara, Thank you.

@souravsarkar4497 3 жыл бұрын

Thanks for making this video

@souravsarkar4497 3 жыл бұрын

Can you prepare ' oxidation value of oil' video ?

@MicroChemsExperiments 3 жыл бұрын

@@souravsarkar4497 You are welcome. Noted the parameter. We will work on it.

@souravsarkar4497 3 жыл бұрын

@@MicroChemsExperiments i couldn't get this video

@MicroChemsExperiments 3 жыл бұрын

@@souravsarkar4497 Why? Which part of this video is not understandable to you? I think it's a clearly explained video.

@souravsarkar4497 3 жыл бұрын

@@MicroChemsExperiments i want to know oxidation value of oil and fat

@vineetpal4789 Жыл бұрын

You are great😂❤❤❤

@MicroChemsExperiments Жыл бұрын

Thank you

@SUARTS143 3 жыл бұрын

Super

@MicroChemsExperiments 3 жыл бұрын

Thank you

@rajesh7999 3 жыл бұрын

You can add the use of test for beginners so they can know immediately...thank you...good experiment channel keep going

@MicroChemsExperiments 3 жыл бұрын

NUTHAKKI RAJESH, thank you for your comment. Surely we will add uses of the test in the video description. Keep supporting us.

@sarahbensoula3378 Жыл бұрын

hello, please can you tell me if i can use this method if i have a really small amount of fat (extracted from a muscle), if not can you recommend me a better method to determine the peroxide value. thank you.

@morszepesi415 Жыл бұрын

Thanks for the great video! Could you please give the different chemical reaction equations for each step? Thanks.

@MicroChemsExperiments Жыл бұрын

Great suggestion! Soon

@zz-kc9mc 7 ай бұрын

Is it inappropriate to stop titration when the solution's top part turns into white?? not the whole part. In our lab, we stop titration when the top part turns into white.. I want to know is there any difference between these two methods.

@nayabghori5934 7 ай бұрын

can we also calculate tocopherols by this titration method ?

@vishmithabsshettigar6700 Жыл бұрын

Was very clear and a nice explanation...Thank you😊 Can you please clarify my doubt regarding how to standardize the prepared solution of sodium thiosulphate with potassium dichromate...Plz🙏

@MicroChemsExperiments Жыл бұрын

Already uploaded in another video. Please find it in this channel

@sujisujith476 Жыл бұрын

Hello.. Is there any relation between free fatty acid and peroxide value?? Means if i get Free Fatty Acid high then should i get peroxide value also high?? Plz relpy

@happyneal1618 2 ай бұрын

May I know thr effecta of Acetic Acid, Potassium Iodide, Water, and Starch soluble in the solution? Also, is the measurement of the reagents added in the solution should be accurate and in order? Thank you.

@thidieuvypham5326 Жыл бұрын

Hi. Thanks for the great video! Could you inform me why we have to use acetic acid-chloroform solution but not other solutions ?

@MicroChemsExperiments Жыл бұрын

AOAC method said so. We will make video later explaining the mechanism of chemical reactions.

@miguelangelruiz1114 4 ай бұрын

Great video and helpme a lot, and so ¿This techique work for oils of animal origin? thanks!!

@MicroChemsExperiments 4 ай бұрын

Yes

@imandyadharmathilaka739 Жыл бұрын

Thanks for this great video! Can you explain how standardized the preparade 0.01N sodium thiosulpate solution with potassium dicromate. How much we need to add?

@MicroChemsExperiments Жыл бұрын

Follow this video: kzfaq.info/get/bejne/jph7paWX07HbYmg.html

@lingalaschool4428 Жыл бұрын

Thank you for ypour video, i need to check peroxide value in macadamia nuts. Do i have to extract the oil from them or i can grind them into poweder and then add distilled water or (what chemical can be added to extract) and shake. which is the easier way. If i have to extract oil which is the method? Thank you

@MicroChemsExperiments Жыл бұрын

If you extract the oil from your sample and do the analysis then it would generate more accurate test results. You can extract the oil by the method of fat extraction using n-Hexane or petroleum ether

@lorenzycodo697 2 жыл бұрын

Hi, how do we calculate the normality of sodium thiosulphate? Thank you for this very informational video.

@MicroChemsExperiments 2 жыл бұрын

kzfaq.info/get/bejne/jph7paWX07HbYmg.html

@keshavtat8471 7 ай бұрын

Yes but confusing me between 0.1 N thiosulphate and 0.01 N thiosulphate Plz tell me how to make 0.01N and standardize.

@Akbar-jw3oi Ай бұрын

Can you please do how to check p anisidine value in oil

@Farmville91 3 жыл бұрын

Can you use acetic acid / Isooctane, instead of the chloroform? And this and all your videos are very HELPFUL!! Thank you!

@MicroChemsExperiments 3 жыл бұрын

Thank you so much for your comment. No, you can't use any other chemical replacing the chloroform in this test.

@iamjhentayoto9662 2 жыл бұрын

Yes.. For safety reason, they allow to use isooctane in replace of chloroform

@RJ10911 2 жыл бұрын

Thanks a lot for making video on this reaction. For few mineral oil we check we didnt get yellow colour after add of ki.so we consider it as a nil. U hv used conc acetic acid. We use diluted as per our sop borrowed fm renowned customer. Will it make diff huge diff.

@MicroChemsExperiments 2 жыл бұрын

You should use concentrated acetic acid as said in AOAC.

@rowidakhalily5760 2 ай бұрын

Plz tall me what is 26.5 . Because you did just one time titration

@QuanMinh-et1dm 2 жыл бұрын

Can I measure the Peroxide Value while the oil is still hot? Thank you for the great video by the way!

@MicroChemsExperiments 2 жыл бұрын

Yes. You can.

@IrfanAli-xy5bf 3 жыл бұрын

Miss kindly share rencidity and FFA testing procedure of shortening. Thanks.

@MicroChemsExperiments 3 жыл бұрын

Irfan Ali, you can find FFA from acid value of an oil sample and Acid Value determination procedure is already uploaded. We will upload Rencidity determination procedure very soon. Thank you for being with us.

@ruchirdave2823 Жыл бұрын

Hello, Thanks for the video it was very informative I want to know whether this method is useful for excipient study?

@MicroChemsExperiments Жыл бұрын

Yes. Useful

@engfatmaabomosa803 3 жыл бұрын

Thanks for your efforts, please can you explain that high value? You know that the value of oil peroxide must be on range from 0.6 to 0.7 in final product , l did the experiment but the value was very high!!!

@MicroChemsExperiments 3 жыл бұрын

High peroxide valo means low quality oil or very rancid oil. The oil that has been degraded its component.

@engfatmaabomosa803 3 жыл бұрын

@@MicroChemsExperiments thank you so much 😍🥰

@vlogsbyzeeshan3526 8 ай бұрын

You didnt proformed this procedure accroding to AOAC method. you must titrate the oil after adding the potassium iodide to remove yellow color and then add the starch solution then again titrate to remove bluish color

@sadiyasajad8176 2 жыл бұрын

Hello mam.. I wanted to know if i had to calculate the peroxide value of meat sample.. Is it that i had to extract the fat using soxhlet method and to that extracted fat only i can go for checking peroxide value... Cant i homogenise the meat samlpe directly in water and check the peroxide value of the homogenised sample..

@MicroChemsExperiments 2 жыл бұрын

You can check peroxide Value directly using the blended meat sample.

@SohelRana-ln7bg 2 жыл бұрын

Pls make the video of preparation & standardization 0.1 N/M of Potassium Dichromatte

@MicroChemsExperiments 2 жыл бұрын

Ok. We will make. Please stay with us

@motivationpk123 2 жыл бұрын

Do you need a blank titration too for the analysis? Some people do a separate titration for the blank sample. Then subtract the titre value of the blank from the sample(Vs-Vb). Before putting that value into the calculation

@MicroChemsExperiments 2 жыл бұрын

Yes. you can conduct blank titration to get the more accurate result. But without blank titration you can also get a acceptable result.

@motivationpk123 Жыл бұрын

Should the blank give a higher or lower titre value?

@dianareyes7371 3 ай бұрын

What if the sample is expected to contain iodine? Would this protocol still be applicable since it is using KI as a reagent? Thank you.

@MicroChemsExperiments 3 ай бұрын

Yes. Same protocol should be followed

@ovaneschakoyan6584 2 жыл бұрын

is it necessary to shake the sample with potassium iodide in dark conditions or this is has no influence on analysis.

@MicroChemsExperiments 2 жыл бұрын

You dont need to keep the sample in dark after adding KI solution.

@mariaph9700 Жыл бұрын

Does it matter if I add the thiosulfate while stirring or not? When I add thiosulfate and wait a little bit versus when I add it while stirring, it yields 2 different results. I am not sure which is right. Please help me explain. thanks

@MicroChemsExperiments Жыл бұрын

Add thiosulfate while stirring

@mariaph9700 Жыл бұрын

How fast should I add it?

@ishaursekar9916 3 ай бұрын

I am using olive oil, and since chloroform is not available in my school, can I use dichloromethane as a solvent instead of the chloroform-acetic acid mixture?

@MicroChemsExperiments 3 ай бұрын

Never find any reference of using dichloromethane. So, no idea

@rohitrajput8241 2 жыл бұрын

Dear sir/mam please make the video of dailation test of vanaspati ghee

@MicroChemsExperiments 2 жыл бұрын

Will try

@arupsarkar525 Ай бұрын

What standard peroxide value ??

@sarahkhan9988 10 ай бұрын

Wow this video is really helpful for me thanks❤!! Can I know why the unit is in per kg, when we use our oil sample in gram for the calculation though?

@MicroChemsExperiments 9 ай бұрын

Look at the calculation again. It was multiplied by 1000 to covert the value into Kg.

@evancbiju474 2 жыл бұрын

Thank u👏Where did that 1000 in the calculation come from?

@MicroChemsExperiments 2 жыл бұрын

Calculation factor

@evancbiju474 2 жыл бұрын

@@MicroChemsExperiments How can we find that?

@hafezquadri2972 3 жыл бұрын

For oils we can use sample directly for analysis.. But could u please tell me how to test a food sample ..any digestion process is required??

@MicroChemsExperiments 3 жыл бұрын

To test a food sample, you have to extract fat or oil content from your food sample first, following our video: kzfaq.info/get/bejne/aJqhirl90p3MmKs.html After extraction of Fat or oil, use the extracted fat/oil to test for peroxide value by following this video.

@hafezquadri2972 3 жыл бұрын

@@MicroChemsExperiments ok thank u..I know about the soxhlet extraction process. Since it is a lenghty process iam looking for any alternative method ...plz do let me know if an other .tq

@MicroChemsExperiments 3 жыл бұрын

@@hafezquadri2972 No other method known to me except extraction of fat

@SwhyBe Жыл бұрын

For sodium thiosulfate I found supplier that sell this solution and its standard already ? Do I need to standardise it after opening?

@MicroChemsExperiments Жыл бұрын

No need to standardise again.

@devanshdesai1811 Жыл бұрын

Thank you for this video. As per ISO and FSSAI manual, there is a step "Titrate with 0.1 N sodium thiosulphate solution with constant and vigorous shaking. Continue titration until the yellow colour almost disappears. Add 0.5 ml of starch solution and continue titration till the blue colour just disappears." AOAC has also mentioned the same. But most of the industries also don't follow this step. They generally do titration directly after adding starch indicator. You also did not followed this step in video. Can you please give some clarity on this?

@MicroChemsExperiments Жыл бұрын

The type of the titration is two steps titration, so it should be completed separately in two steps to get the more accurate results as suggested by AOAC.

@arslanmunir9334 Жыл бұрын

I am facing the problem related to Indicator. When I add 1% starch indicator ,No Color change observe. Can you please resolve this issue OR give any suggestion?

@arslanmunir9334 Жыл бұрын

As I use freshly prepare KI and Starch Indicator.

@factxfact5549 3 жыл бұрын

Is peroxide value test enough to check if the oil is rancid or not?

@MicroChemsExperiments 3 жыл бұрын

No. Not enough. Peroxide value just gives an primary indication of Rancidity. You must go for the rancidity test to confirm. We have recently uploaded Rancidity test video. Plzz follow that video for details about rancidity test.

@humble8432 3 жыл бұрын

What research question can be formed out of this experiment for IB experiment?

@MicroChemsExperiments 3 жыл бұрын

Thanks for asking tha question but we have only concern with the experiments, relating our videos. We have no idea about questionnaire and we are very sorry for that.

@chernora.mconteh5449 6 ай бұрын

Please can you assist me with the soft copy of this method

@prescilliadianemamboundou7338 3 жыл бұрын

Hello, I tried to reproduce this method in lab without adding sodium carbonate in the preparation of sodium thiosulphate. However, I didn’t observe the end point. Should I increase the concentration of sodium thiosulphate?

@MicroChemsExperiments 3 жыл бұрын

1. Prepare sodium thiosulphate according to: kzfaq.info/get/bejne/jph7paWX07HbYmg.html 2. Use freshly prepared Starch and potassium iodide solution. No need to increase the concentration of sodium thiosulphate. Just follow this video strictly and continue titration until the end-point is achieved

@prescilliadianemamboundou7338 3 жыл бұрын

@@MicroChemsExperiments ok thanks

@prescilliadianemamboundou7338 3 жыл бұрын

@@MicroChemsExperiments please Ma’am, for the conversion of meq/kg to mmol/L. Which density should I use?

@luzdelunalopezaleman9260 10 ай бұрын

Hola, buenas tardes. Podría aclararme una duda que tengo?

@MicroChemsExperiments 9 ай бұрын

Yes. Sure. Write us at mail.mic.chem@gmail.com

@shraddhapiligaonkar3639 Жыл бұрын

Is there any replacement for chloroform can dichloromethane be used in place of chloroform?

@MicroChemsExperiments Жыл бұрын

Not sure and no replacement to be known actually.

@nonavancoillie900 Жыл бұрын

For acetic acid, can you also use the acetic acid you use in the kitchen?

@MicroChemsExperiments Жыл бұрын

No. The acetic acid in your kitchen is in food grade quality. In analytical laboratory, always use analytical grade of chemicals.

@arslanmunir9334 Жыл бұрын

Which type of starch is use as an indicator? It available as rice, potato and other starch. Can you please specifies its type?

@MicroChemsExperiments Жыл бұрын

Use reagent grade starch which is soluble in warm water

@halaawwad2143 2 жыл бұрын

Should you do the plank experience? If so, how?

@MicroChemsExperiments 2 жыл бұрын

Please clarify your question

@azaruameer3931 2 жыл бұрын

Can you give me the "Determination of Oil Analysis" manual if I need it?

@MicroChemsExperiments 2 жыл бұрын

We do not provide any document

@noorinoora2627 Жыл бұрын

Why when i put starch the sample not turning blue/ black it just white

@MicroChemsExperiments 11 ай бұрын

Prepare chemicals freshly and try again

@doobydoes4956 Жыл бұрын

I read you can use cyclohexane for the organic solvent instead of chloroform. Is that true?

@doobydoes4956 Жыл бұрын

CYCLOHEXANE DOES INFACT WORK. YOU NEED TO PIT IN 3 GRAMS OF SAMPLE, 50ML ORGANIC SOLVENT AND 100ML WATER 🔥

@MicroChemsExperiments Жыл бұрын

Yup

@SwhyBe Жыл бұрын

I have tested body oil of brand and peroxide value is 34 does that mean is not good in quality ?

@MicroChemsExperiments Жыл бұрын

Yes. Not good in quality of that oil

@munmunkundu9033 Жыл бұрын

Can i do this experiment with maize parts

@MicroChemsExperiments Жыл бұрын

No idea. You can try

@isurikavindiamarasingha855 Жыл бұрын

I tried this one but when I added starch solution, no blue or black colour produced. What is the reason for that?

@MicroChemsExperiments Жыл бұрын

Prepare your reagents freshly and try again. If same result found, you can interpret the result as 'Nil'

@killjadeskye 8 ай бұрын

Anyone here have done Peroxide Value for virgin coconut oil? Does your sample turn black?

@ajforeversmiley2641 3 жыл бұрын

When I add the 1% starch solution, there is no colour change. What am I doing wrong?

@MicroChemsExperiments 3 жыл бұрын

Ajforeversmiley, Possible two reasons for no color change after adding 1% starch are given below: 1. May be the starch solution is not freshly prepared. 2. May be your sample have very low peroxide value.

@islascel2652 3 жыл бұрын

@@MicroChemsExperiments If the color does not change even though I prepared it fresh, is the starch powder I use spoiled?

@MicroChemsExperiments 3 жыл бұрын

@@islascel2652 No, we can not say directly that starch powder is spoiled if color does not changed. when we add starch solution, amylose forms a blue to black solution with iodine. So if your sample does not contain iodine then black color will not be formed. The peroxide value is determined by measuring the amount of iodine which is formed by the reaction of peroxides (formed in fat or oil) with iodide ion. So, if color does not changed, interpret the Peroxide value result as 'Nil'

@motivationpk123 3 жыл бұрын

You could try heating the starch solution before adding it. Worked for me

@29moch.syarifbisma.a6 2 жыл бұрын

@@MicroChemsExperiments Btw When i added Starch first before Distilled water, the black/blue colour is appear Is that true?

@umaimakamal9795 2 жыл бұрын

My teacher asked what is the relationship between cloud point and melting point do u know?

@MicroChemsExperiments 2 жыл бұрын

Take help from Google

@oumaimaazakak7285 3 жыл бұрын

Hello Miss, please I wanna know the average of peroxide value that we can get in our experiment for known is the oil have a good quality or not and thanks in advance.

@MicroChemsExperiments 3 жыл бұрын

Lower peroxide value indicates the good quality of oil. Quality decreases with the increasing peroxide value.

@oumaimaazakak7285 3 жыл бұрын

Thank you so much 🥰

@oumaimaazakak7285 3 жыл бұрын

I have another question about the blank, when I use a blank in this experiment and start my titration on it With thiosulfate of sodium what's the value shall I get when my blank pass to a colorful solution to a colorless solution.

@MicroChemsExperiments 3 жыл бұрын

@@oumaimaazakak7285 It varies with many conditions or parameters. Plz conduct the test in your lab to get the more idea.

@sashankkunwar2222 2 жыл бұрын

HELLO CAN WE MAKE SODIUM THIOSULFATE OF O.05N BY THIS DOWN METHOD. i.e NEV/1000 =0.05×40×1000/1000 =2gm in 1000 ml distilled water

@MicroChemsExperiments 2 жыл бұрын

Follow this video: kzfaq.info/get/bejne/jph7paWX07HbYmg.html

@parmjeetsingh4167 3 жыл бұрын

Dear madam please make a vedio for wijj solution ..how it impact iodine value...

@MicroChemsExperiments 3 жыл бұрын

Okay. I will make video on it as early as possible. Stay with us.

@sweathaswea4660 2 жыл бұрын

When I m adding starch solutions tha sample colour not turn black colour it will be in yellow (for ghee sample ) give me solutions

@MicroChemsExperiments 2 жыл бұрын

Prepare potassium Iodide and Starch solution freshly on the day of analysis.

@SohelRana-ln7bg 3 жыл бұрын

How to standardize sodium thiosulfate by patassium dichromate?

@MicroChemsExperiments 3 жыл бұрын

Link: kzfaq.info/get/bejne/jph7paWX07HbYmg.html Did you watch my video list. Please search your asking test from my video list.

@SwhyBe 3 жыл бұрын

Great video can please mention the concentration % of acetic acid

@MicroChemsExperiments 3 жыл бұрын

We used Glacial Acetic Acid (99.5%)

@SwhyBe 3 жыл бұрын

@@MicroChemsExperiments perfect one more question can I purchase 0.0109N solution already prepared and standardised will it work for this experiment?

@MicroChemsExperiments 3 жыл бұрын

@@SwhyBe Yes. You can purchase 0.01N solution from local market and can use in this experiment. If you face any difficulty to purchase they follow our video for the preparation and standardization: kzfaq.info/get/bejne/jph7paWX07HbYmg.html

@SwhyBe 3 жыл бұрын

@@MicroChemsExperiments its not 0.01N actually it is 0.0109 N will this effect the experiment?

@MicroChemsExperiments 3 жыл бұрын

@@SwhyBe No problm with it

@newsofislam1284 Жыл бұрын

After adding Saturated KI solution may need to insert corck immediately?

@MicroChemsExperiments Жыл бұрын

Yes

@newsofislam1284 Жыл бұрын

You are not showing that.

@MicroChemsExperiments Жыл бұрын

@@newsofislam1284 That was our error occurred to make the video faster. But you should follow what you said in your lab

@newsofislam1284 Жыл бұрын

Thanks a lot.

@chuqiaomai290 3 жыл бұрын

Can you use this method to determine peroxide value in dried milk powder?

@MicroChemsExperiments 3 жыл бұрын

Yes. You can

@chuqiaomai290 3 жыл бұрын

@@MicroChemsExperiments Great thank you so much! Do I need to extract fat from the powder first or the method can be applied to milk powder directly?

@MicroChemsExperiments 3 жыл бұрын

You have to extract fat first using Hexane or Ether solvent. Then go for the analysis

@sweathaswea4660 2 жыл бұрын

Can we use for titration 0.1 n sodium thiosulfate

@MicroChemsExperiments 2 жыл бұрын

No. If you use 0.1N instead of 0.01N then you will not get the accurate result of peroxide value.

@trucminguyen9510 2 жыл бұрын

Please help me explain, In this test, do we need to titrate the blanc sample? This video are very helpfull, thank you so much.

@MicroChemsExperiments 2 жыл бұрын

Yes. We need to tritrate our sample after dissolving in ethanol by heating.

@trucminguyen9510 2 жыл бұрын

@@MicroChemsExperiments how to titrate the blanc sample? Can i change sample test to distill water

@kimmytran9973 2 жыл бұрын

@@trucminguyen9510 in my lab we titrate the blank the same as the sample, except that there’s no oil added. Blank reading less than 0.05ml is acceptable in my lab.

@trucminguyen9510 2 жыл бұрын

@@kimmytran9973 thank you so much

@EliElordi Жыл бұрын

@@kimmytran9973 hi, may I know how your side prepare the blank? I did follow the method but my blank always end up higher than 0.10, often I get around 0.30 to 0.70ml which is quite high. The procedure I use is as follow: 1) 50ml acetic acid/isooctane into 250ml flask 2) add 30ml reagent water, swirl to mix and add 1ml saturated KI 3) Add 0.50ml starch (blue colour appears) and titrate to endpoint (white). What would cause my blank to be so high? I did prepare new starch and new KI solution but still can't get low blank

@MrLongKR Жыл бұрын

Which AOAC method is the reference for this?

@MicroChemsExperiments Жыл бұрын

Reference is given in the video title

@MrLongKR Жыл бұрын

@@MicroChemsExperiments oh yeah! Sorry I missed that! Thank you

@MicroChemsExperiments Жыл бұрын

@@MrLongKR no problem sir. Thank you

@lorenzycodo697 2 жыл бұрын

please please can you explain how to standardise the sodium thiosulphate

@MicroChemsExperiments 2 жыл бұрын

kzfaq.info/get/bejne/jph7paWX07HbYmg.html

@raogamingyt9957 2 жыл бұрын

Sir when you add starch then solution color changed. But I perform same procedure and when I add starch solution my sample color not change.sir plz tell me what's the matter.

@MicroChemsExperiments 2 жыл бұрын

Peroxide Value is nil

@raogamingyt9957 2 жыл бұрын

@@MicroChemsExperiments thanks for rply.

@MicroChemsExperiments 2 жыл бұрын

@@raogamingyt9957 you are welcome

@A-Z.Unlimited 3 жыл бұрын

can i use more (such as 3 or 4 mL) than 1mL of Saturated KI?

@MicroChemsExperiments 3 жыл бұрын

A-Z Unlimited, AOAC method says to add 1ml Saturated KI. So we should follow the reference method strictly to get the accurate result. But you can add 3-4 ml KI and the test results will not differ significantly.

@A-Z.Unlimited 3 жыл бұрын

tnx@@MicroChemsExperiments

@oumaimaazakak7285 3 жыл бұрын

@@MicroChemsExperiments I'm so glad for founding this useful channel, I learn a lot of things in how I can analyzing the quality of oils, thanks a lot, but please Can I know what the role or why we using KI in this procedure?

@MicroChemsExperiments 3 жыл бұрын

@@oumaimaazakak7285 In a short comment, it is not possible to explain chemical reactions. Later, we will make video for each test explaining the mechanism of rections. Till then stay with us. Thank you.

@lorenzycodo697 2 жыл бұрын

Hi, how can I conduct a blank titration?

@MicroChemsExperiments 2 жыл бұрын

Follow the same procedure except taking sample in the flask

@SharifulIslam-kb7jd 3 жыл бұрын

Your caption says you are determining Peroxide value but during the calculation you said acid value 4.09 meq/kg

@MicroChemsExperiments 3 жыл бұрын

It was a slip of tongue. You did mistake in sayings. It should be peroxide value. We actually determined peroxide value in this video. We are very sorry for our mistake.

@29moch.syarifbisma.a6 2 жыл бұрын

In my Lab. While shake/mix The Erlen must be Close with Tube for erlenmeyer Is that True?:/

@MicroChemsExperiments 2 жыл бұрын

Yes. True

@29moch.syarifbisma.a6 2 жыл бұрын

@@MicroChemsExperiments can you give me the reason, why we do that? :/

@MicroChemsExperiments 2 жыл бұрын

@@29moch.syarifbisma.a6 Volatile compounds are form in the flask afrer mixing the chemicals with samples. Cap should be used to avoid the escape of the volatile matter from the flask.

@29moch.syarifbisma.a6 2 жыл бұрын

@@MicroChemsExperiments oh ok, thank you :D

@MicroChemsExperiments 2 жыл бұрын

@@29moch.syarifbisma.a6 You are welcome

@udaykumarkachrola7494 2 жыл бұрын

Why you add .02g Sodium carbonate in Sodium thiosulfate solution ?

@MicroChemsExperiments 2 жыл бұрын

AOAC method said so

@bablubadmash9408 2 жыл бұрын

0.5 g starch soluble added in how much ml of water?

@newsofislam1284 Жыл бұрын

50 ml

@jancy98 11 ай бұрын

Can u plz share the calculation for the amount of sodium thiosulphate to taken in reagent preparation?

@MicroChemsExperiments 11 ай бұрын

Please take help from Google

@mahedihasan-fq2oc 10 ай бұрын

I need 0.01 sodium thiosulfat solution

@rashibhasan873 3 жыл бұрын

Dear, Mam. I prepared Sodium thiosulphate (0.01N) solution using your formula and tried to determine the peroxide value.But Blue color of the final mixture is not changing even after adding more than 100 ml (Na2S2O3.5H2O). Then my prepared soln even is not working during standardization of sodium thyosulphate by K2Cr2o7. Where is the problem mam,Is there any problem in Na2s203.5H2O solid.

@MicroChemsExperiments 3 жыл бұрын

Yes. May be there is a problem with your sodium thiosulfate crystal. It should not be happened what you said. Otherwise there is problem in glassware and apparatus cleaning. Plzz watch the video again and do the experiment again using clean glassware and apparatus and preparing chemicals and reagents accurately & freshly. After that let us know the result. We will try to find the problem and give you a solution.

@rashibhasan873 3 жыл бұрын

@@MicroChemsExperimentsi tried and still trying to solve the problem. I cleaned my apparatus using 32% HCL, then washed several times with Distilled water and dried using hot plate and the burette was rinsed by the sodium thyosulphate solution. Using your video I determined acid value successfully but in determining peroxide value it is not working. I used freshly prepared KI and starch indicator and as the solution turns to blue implies that both KI and starch indicator are in good condition but problem crops up when using sodium thyosulphate. So where is the problem.

@MicroChemsExperiments 3 жыл бұрын

@@rashibhasan873 Test another sample brought from local market and test again. If your problm is not solved then replace sodium thiosulfate reagent by a new one of good brand & supplier.

@rashibhasan873 3 жыл бұрын

@@MicroChemsExperiments I conducted the experiment several times but it is not working.I am currently using sodium thyosulphate pellets imported from Merck specialities Private Ltd, Worli, India. Which brand should I use instead to get over this problem?

@MicroChemsExperiments 3 жыл бұрын

@@rashibhasan873 before changing the chemicals, conduct a test for another saybean oil sample purchased from the local market.