Solid Phase Extraction (SPE) Tutorial

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Күн бұрын

Ashmeeta and Matt are here to answer all your Solid Phase Extraction (SPE) confessional questions!
In this episode of Sample Prep Confessions, Matt and Ashmeeta break down the reasons labs use SPE, the steps of SPE, advantages of SPE, and finally a demonstration of how solid phase extraction is accomplished using dye coloring.
To learn more about solid phase extraction (SPE), click the link in the video or right here! www.phenomenex.com/SPE. You can also reach out to our Technical Experts through Live Chat nearly 24 hours a day any where in the world! Check it out today: www.phenomenex.com/chat

Пікірлер: 35

@ssss-cz1nf 2 жыл бұрын

I've read it one million times in my theory book of analysis and with a 8 min video I understood it better than ever

@saileshkatara686 3 жыл бұрын

Seriously this is the best video on yt as of now for spe

@richardwells5390 2 жыл бұрын

Really helped me understand this concept better for my lab class. My instructor sent me a video which was very difficult to understand, but this was much more comprehensible. Thanks

@riazhosein2394 5 жыл бұрын

Really awesome job guys! Thank you!

@cuterobot7836 3 жыл бұрын

Thank you this help out to undestand better SPE . Really nice moderators

@douglashurd4356 2 жыл бұрын

Thank you, very clear demonstration. Also, it was nice to have Ashmeeta there to represent the human species! 😀

@ucjakozicka978 11 ай бұрын

love the explanation thank you!

@manuelfrn 2 жыл бұрын

Nice work!

@woromurtiutami1527 5 жыл бұрын

Thank you, iam an undegraduated student, and i use this methode to separated my peptide sample

@Phenomenex 5 жыл бұрын

We are glad if it was helpful! Please let us know if there are any other demonstrations you would like to see in the lab.

@AE-ben 5 жыл бұрын

Nice!!!

@gianlucafarina2825 3 жыл бұрын

First of all, thank you very much for the tutorial. It is really useful. I wanted to ask if the conditioning of the direct phase columns is the same. Thanks so much again!

@Wei-yv1pp Жыл бұрын

Thanks!

@richardjohnson7746 2 жыл бұрын

Great video! I think the best so far I have watched. But I would like to ask a question: Is it advisable to use MeOH throughout if acetonitrile is not available?

@nkhumelenimulalo6741 3 жыл бұрын

A really good and detailed video. I am just wondering if i need a large amounts of samples how do i go about. Like if my sample introduction system requires atleast 10m of a sample.

@sunnyrainyday6820 3 жыл бұрын

Im a Forensic chemistry student and if never done a Solid Phase Extraction. tho it does look very similar to a Resin Exchange Chromatography experiment that i've done but fortunatly for the sake of time the experimenter could control flow

@shabinafatma562 Ай бұрын

I want this manual Solid phase extraction kit I.e. Spe cartridge and push rod. Can you provide me the complete set up at least cost

@user-qm6mw6el4g 3 ай бұрын

Hi is it possible to use this method for the determination of concentration of caffeine

@aqdusshaikh1306 Жыл бұрын

Hey, can we perform this for extraction of Isoquercitrin from petals of Dianthus.pavonius?

@137bob3d 3 жыл бұрын

the column of C18 is only a cm or 2 in length. far shorter than the normal phase columns i've seen on vid's here on u-t. could you comment on this feature of C18 columns , please

@francoisburgay9002 4 жыл бұрын

Before eluting the samples, do we have to dry the cartridge?

@Phenomenex 4 жыл бұрын

While you don't have to, it is an option to dry the cartridge before eluting the samples. By taking this step it helps further disrupt the stationary phase/analyte interaction to promote elution.

@137bob3d 3 жыл бұрын

the chatter ends at 3:38 ... then the experiment begins

@danielataylor6292 5 жыл бұрын

What solvents are the dyes originally in?

@Phenomenex 4 жыл бұрын

Hi! Great question, for this demonstration we used an aqueous based dye solution.

@danielataylor6292 4 жыл бұрын

@@Phenomenex thanks

@AndyU96 3 жыл бұрын

@@Phenomenex Is that why you passed water through the column before you injected the samples?

@joshr.5199 4 жыл бұрын

im sure its totally fine, and im just weird, but the micropipette sitting horizontally with the tip on drove me a little crazy

@Phenomenex 4 жыл бұрын

Hey Josh, we are so sorry about that! Which part of the video are you referring to? Because I am sure that would make us a little crazy too.

@ShOOtinGliTteR 4 жыл бұрын

@@Phenomenex 3:41

@ShOOtinGliTteR 4 жыл бұрын

4:03 to almost 5:00

@ShOOtinGliTteR 4 жыл бұрын

and again at 5 02

@jimseldiesel1362 2 жыл бұрын

why is your micropipette HORIZONTAL IN 4:18 noooOOOOO

@slytherinda4669 2 жыл бұрын

it only matters if there is fluid in the tip

@AndyU96 3 жыл бұрын

THIS is solid phase extraction? It's just a typical column chromatography for god's sake. Why is it not just called column chromatography?