Thin Layer Chromatography and Retention Factor

Рет қаралды 61,061

Andrey K

Күн бұрын

Пікірлер: 39

@mohammedabdulmannan6746 8 жыл бұрын

your explanation is very simple and clear. Thanks a lot brother.

@bios546 9 жыл бұрын

A really good and a clear lecture. Honestly... u have no parallel... I do mean each word!. I understood everything in one go!. Now, I have some doubts, 1) Do we always have to use a rectangular plate? 2) Why doesn't the retention factor depend upon the thickness of the plate? 3) Can we calculate the kinematic variables of the rate of rise? 4) What are the factors that affect both the products found in the mixture and the solvent that affect the acceleration and retardation of the respective compounds? 5) In the solvent and solute in the example given, is there an initial acceleration? if yes, upto which position does it accelerate? and then, how will the deceleration depend upon the acceleration?

@bensyblair-rb5ix Жыл бұрын

Thanks so much sir ,I haven't understood this from my teacher but I got it clearly today thanks so much I will keep on following you

@yahyanasso4130 3 жыл бұрын

I love u so much, honestly u are a genius. ur explanations are just perfect.

@SatyaMuslimah95 8 жыл бұрын

your explanation is very easy to understand.. thank you so much sir :))))))

@Shocase91 7 жыл бұрын

thank you, you are a great lecturer

@LADYGRASY 11 ай бұрын

Does the mobile phase contains a solvent and an analyte Does the stationary phase contains a column and solvent?

@rassimsimou1594 Жыл бұрын

Good

@saifchaudhary4993 2 жыл бұрын

Thanquhh so much you should teach at my college in place of my professor

@reemqurban6651 6 жыл бұрын

Very clear explanation. Thank you

@bawakuddy4807 6 жыл бұрын

This guy is good.

@rakshithasharma74 7 жыл бұрын

how can solid material will be stationary phase

@lynny7868 6 жыл бұрын

Much respect!!

@saifchaudhary4993 2 жыл бұрын

Superhumans do exist like him

@esraaelkherwilly9163 7 жыл бұрын

Many thanks ❤❤❤❤❤

@raghadhammami5364 4 жыл бұрын

Thanks ❤️❤️

@francistelferjnr2802 Жыл бұрын

So where does paper chromatography fall

@nayabshah88 6 жыл бұрын

thanks

@infinitefuture1828 2 жыл бұрын

Could you please do one on HPLC?

@jahanararahman847 7 жыл бұрын

You presentation materials are very informative but your lectures are so fast that its really hard to understand!

@effasvt_ 5 жыл бұрын

Is Rf1 and Rf2 are value from the same compound?

@saichaitanya3780 7 жыл бұрын

AMAZING EXPLANATION SIR

@MooseM3 6 жыл бұрын

This dude is so knowledgeable; he has helped me a lot in ochem/biochem, but I kind of can't take the way he is screaming at me... it's almost too much lmao

@IScreamer 2 жыл бұрын

You keep mentioning that the stationary phase is the plastic plate. It’s not. It’s simply a means of supporting the stationary phase which is a highly polar substance. A very common stationary phase in TLC is silica gel. So, in such a case, it’s the silica gel which is the stationary phase and the components of any mixture added to that phase will be adsorbed, or bound to the silica gel to varying degrees, depending on the polarity of the individual components. Less polar components will travel up the plate at a greater rate than more polar components which will be more strongly attracted to the surface of the stationary phase. Another common support for the stationary phase is glass.

@lgnobil 2 жыл бұрын

Yep, and it could also be alumni for TLC which is slightly less polar than silica. Anyway what confuses me the most about TLC is that the stationary phase is highly polar, and for our substances to travel up the plate they need to be soluble by the mobile phase, which could be polar or non-polar, so if our mobile phase is non-polar and the substance is polar, then it will not be dissolved by the mobile phase and it will have high attraction to the stationary phase so it really won't move much or at all, however if our substance is non-polar, and our mobile phase is polar meaning it still won't dissolve the substance, then that means the substance doesn't have attraction to either of the phases, so what happens? Does our retention factor stay at or close to 0 as well in this case?

@kalpshah3273 6 жыл бұрын

God level

@RoXon007 6 жыл бұрын

This is in biology course not chemistry

@lynny7868 6 жыл бұрын

Your view is too narrow. I've took IB Bio and AP Chem and both courses cover this topic.

@RoXon007 6 жыл бұрын

@@lynny7868 ohh

@SolaScriptura49 6 жыл бұрын

We are addressing this topic in Organic Chemistry Lab now. I do not remember this being taught in Bio I or II. It would be interesting to see what unit in Bio you were taught.

@RoXon007 6 жыл бұрын

@@SolaScriptura49 we are studying this in college in biology chapter 1of first year and chapter name is cell structure and function. This is in the topic of techniques for studying cell structure

@SolaScriptura49 6 жыл бұрын

Very interesting, I do not remember that being a topic in bio I, what a great introduction though!! We just finished recrystallization and are now in solubility in orgo. I would say this is used both in bio and chemistry.

@rakshithasharma74 7 жыл бұрын

how can solid material will be stationary phase

@namrasartajkhan661 4 жыл бұрын

Hope so bro you are clear now with this point after two years 😂..

@saifchaudhary4993 2 жыл бұрын

Hope so you are clear now with this question after 4 years😂😂