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Determination of Acid value & Free Fatty Acid(FFA)_A Complete Procedure (AOAC 940.28 & ISO 660.2009)

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MicroChem's Experiments

MicroChem's Experiments

Күн бұрын

Пікірлер: 391
@emanroshanara3799
@emanroshanara3799 10 ай бұрын
Tomorrow is my practical and it is helping me alot From Pakistan
@MicroChemsExperiments
@MicroChemsExperiments 10 ай бұрын
Glad to know
@chemistbd886
@chemistbd886 2 жыл бұрын
Thanks a lot such types of video uploaded. I see and saw your all video and i feel very happiness. Great wishes for you and your channel.
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Thanks.
@bellabconcept1432
@bellabconcept1432 Жыл бұрын
Thank you for complete step and most easiest method you are using. I never regret of knowing this great Institute of Learning. Nevertheless, free fatty Acids determination was not done in this video
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Thanks. Free Fatty Acid calculation is given in the description section. Please take a look
@buonyduopkuek
@buonyduopkuek 2 ай бұрын
Thank you for your upload videos and I'm very interested to learn that teaching for easily understanding!!!!❤❤❤❤ I'm new for your channel and I will introduce my position to you!!! I'm one among chemistry student: 1. Under graduate, 2. Bachelor degree, 3. 4th year Thank you so much and I will follow you always !!!!!
@frankcastle4657
@frankcastle4657 3 жыл бұрын
MicroChem's Experiments people, I just saw your posts today and you are such smart, human angels for sharing your knowledge in such a simple and easy way to understand. Thank you so much! May God/Karma/Life blesses you and your loved ones today and always! Do you have any videos of how to prepare samples, standards, control, and blanks for ICP-OES? And how to do analysis with a GC and an UV? Thank you, you are appreciated!
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Thank you very much for your appreciations. We do not have video for ICP-OES. In future, we will try to make video on it. We will upload video on GC soon. Stay with us.
@sachinap7141
@sachinap7141 2 жыл бұрын
I am microbiology test only analysis,but your video will help to learn Crude protein analysis,crude fiber and crude fat test is very easy and result is very good
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Thank you soo much for such appreciation. We uploaded standard preparation for AAS. We. Also uploaded one video for GC and several video for spectrophotometer analysis. In future we will upload for ICP-MS too. Stay with us. Soryy for late reply.
@abigailabu9299
@abigailabu9299 5 ай бұрын
Very educating and simple too, but please how do I estimate the free fatty acid from the acid value dealing with Shea butter sample
@IrfanAli-xy5bf
@IrfanAli-xy5bf 3 жыл бұрын
Miss thanks for sharing, yours methods are so easy which helps easy understanding. Keep it up.
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
My pleasure 😊
@vipinpathak4831
@vipinpathak4831 3 жыл бұрын
Oleic base oil me factor 40 nahi 28.2 hota hai ffa ke liye Acid value 2 multiple of ffa factor matlab 56.1 hoga Calculation ffa= b. Reading *28.2*normality of naoh --------------------------- Weight of taken sample
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Vipin Pathak, you are right. NOTE: There is a mistake in the formulae of acid value, mentioned in this video, and the formulae for FFA is not given in this video. Both corrected formulae & calculations of Acid value and FFA are given below: *Acid value (as oleic acid) = (56.1×V×N )/W So, Acid Value = 1.6 *Free fatty acids (as oleic acid) = (28.2×V×N )/W So, FFA = 0.805 percent by weight
@dvnv6250
@dvnv6250 2 жыл бұрын
@@MicroChemsExperiments hi may i know what is the 28.2??
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
@@dvnv6250 calculation factor
@luckynamkeenfryums
@luckynamkeenfryums 6 ай бұрын
Right ho aap Aap kha se ho bhai
@vipinpathak4831
@vipinpathak4831 6 ай бұрын
@@luckynamkeenfryums modinagar ghaziabad
@PrashantPatil-hs5rr
@PrashantPatil-hs5rr 2 жыл бұрын
Something is not right I think formula molarity mentioned 40 but actual sodium hydroxide 28.2 is right
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Corrected Calculation is given in the description section of this video
@vidhyav8650
@vidhyav8650 8 ай бұрын
my keen request to post a video on lipase estimation by titration method for flour samples .....
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
Will try
@harishankarpatel8716
@harishankarpatel8716 Жыл бұрын
Thanku so much Mam Acid value of oil experiment ke liye🙏🙏🙏🙏🙏
@anjumanara6967
@anjumanara6967 11 ай бұрын
Can I use this method to know bacterial acid value? And my lipid content of bacteria is. 0085gm then the procedure will be the same? Like 50 ml ethanol etc
@MicroChemsExperiments
@MicroChemsExperiments 10 ай бұрын
No idea about your sample. You can try and let me know the results.
@faijtrds
@faijtrds 3 жыл бұрын
Thanks so much Maam For giv6 In detail knowledge for us
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
You are most welcome.
@putrikaurong9292
@putrikaurong9292 Жыл бұрын
Is it okay if im using methanol instead of ethanol?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
No. That will be wrong
@TijanaVasiljevic-nd8fx
@TijanaVasiljevic-nd8fx 10 ай бұрын
This is great! Thank you :D
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
Glad you like it!
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
NOTE: There is a mistake in the formulae of acid value, mentioned in this video, and the formulae for FFA is not given in this video. Both corrected formulae & calculations of Acid value and FFA are given below: *Acid value (as oleic acid) = 56.1×V×N --------------- W So, Acid Value (mg/g) = 1.6 *Free fatty acids (as oleic acid)= 28.2×V×N --------------- W So, FFA = 0.805 percent by weight
@AriffMahtar
@AriffMahtar 3 жыл бұрын
i have noticed in the early video, thank you for correction!
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
@@AriffMahtar Actually we did a silly mistake in the calculation. Please ingore it.
@crayversace2804
@crayversace2804 3 жыл бұрын
Isn't 56.1 the molecular weight for KOH.. And you used NaOH in the experiment
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
@@crayversace2804 AOAC method said to use 56.1 as the factor of this calculation for in both of the cases whether you use 0.1N KOH or 0.1N NaOH solution.
@crayversace2804
@crayversace2804 3 жыл бұрын
So what would the formula be if I used 0.1 KOH, 10 grams of oil, and had a titration volume of 0.76
@tasbeehghareeb5931
@tasbeehghareeb5931 3 ай бұрын
Thanks a lot for your effort. What is the range of acid value?
@vidhyav8650
@vidhyav8650 8 ай бұрын
hello ..i have a doubt ....to get FFA in percentage wew have to use the same formula and also FFA does not have any unit....another question can i use formula for the estimation of FFA in Pearl millet flour..? kindly give me a suggestion...
@shubhamrawat8028
@shubhamrawat8028 10 ай бұрын
UTU Chemistry lab teacher........🚀🚀
@MicroChemsExperiments
@MicroChemsExperiments 10 ай бұрын
Appreciate 👍
@sarakhalid6469
@sarakhalid6469 2 жыл бұрын
Thanks for those videos❤️‍🔥❤️‍🔥
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You are welcome
@tsmtvipergaming6819
@tsmtvipergaming6819 3 жыл бұрын
very informative video...
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Rahul Pawara, thank you so much. Be with us.
@dhruvkadivar2312
@dhruvkadivar2312 Жыл бұрын
Hello sir/mam, First of thank you so much your videos is valuable for foods company’s. I have two help from your side so 1st questions > how to determination of oil% & protein in Groundnut oil cake ? 2nd questions > how to determination of oil % in Groundnut seed (Peanut) ? Please sir/mam i hope you are making this two questions videos share on youtube…
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Please follow our video for the Determination of crude protein and crude fat. Search on our channel to find those videos. I think you will find them easily
@Cookie-wt4uz
@Cookie-wt4uz 2 жыл бұрын
What is the difference between ffa value and acid value? And also mass of oleic acid is 282.5 so why 28.2?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Take help from Google for detailed explanation
@muhammadaltaf9651
@muhammadaltaf9651 3 жыл бұрын
Excellent video
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Thank you so much. Stay with us
@mysticforest6345
@mysticforest6345 2 жыл бұрын
Hello, I have some questions. What is the difference between normality and molarity? What is the purpose of standardizing the solution? Finally, why do we need to do the first titration of the ethanol?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
1. You can find the difference between normality and molary from google. 2. Purpose of stadardization is to know the exact concentration of any solution. 3. Normally ethanol is slightly acidic. So first titration of ethanol is done to make the ethanol neutral as if it does not interfare the acidity of our oil sample.
@mysticforest6345
@mysticforest6345 2 жыл бұрын
@@MicroChemsExperiments So for every trial of this procedure, do I need to standardize the solution every time?
@mysticforest6345
@mysticforest6345 2 жыл бұрын
Also, may I ask where you got the expected acid values from? I need a citation. Can you please also explain the formula in the calculation section? Thank you very much for your help. I appreciate it :)
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
@@mysticforest6345 No. But standardize when you will analyze your sample on a different day.
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
@@mysticforest6345 Search on Google and find the reference value from journals and publications. You can also find it from countrys standard of that sample.
@SohelRana-ln7bg
@SohelRana-ln7bg 3 жыл бұрын
But the oil samples doesn’t mix after boiled
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Boiling of oil with ethanol will not facilitate to dissolve the oil completely. So no worries about it. Perform the test according to our video. You will get the accurate result.
@lewii0212123
@lewii0212123 Ай бұрын
How to avoid measuring mineral acids (e.g. sulfuric acid) and organic acids, only I would like analyze Free fatty acid content.??
@user-if7df1rm2w
@user-if7df1rm2w Жыл бұрын
When I needed to get Acid value in our laboratory for fats I used 0.1 N NaOH for titration , but when I calculated I multiple by 56.1 . our specification in our laboratory said that. Is that right?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Right
@user-if7df1rm2w
@user-if7df1rm2w Жыл бұрын
But why ? Please give me the reason I think when you titrated with 0.1 NaOH you multiple by 40.001. so I said to my boss that our specification is wrong because we multiple by 56.1
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
@@user-if7df1rm2w if you follow AOAC method, it is written that you have to use factor 56.1 whether you use KOH or NaOH
@Lila-lc7wd
@Lila-lc7wd 7 ай бұрын
Hi, thanks for the video. Why do we boil the ethanol-sample solution and what percent phenolphthalein solution are you using? Also, can I neutralize ethanol using KOH instead of NaOH? Thanks again!
@samsciences5966
@samsciences5966 2 жыл бұрын
great work
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Thank you
@Foodtechie000
@Foodtechie000 23 күн бұрын
How to find out free fatty acid value in cake mix?
@sonofasnitch2172
@sonofasnitch2172 3 жыл бұрын
i wanna ask, we do the titration while the mixture is still hot or not?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Son of snitch, do titration while it is hot.
@ayeshanajam7001
@ayeshanajam7001 2 жыл бұрын
Thanks for sharing easy way to learn. Why you boiled the sample
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
For proper mixing of oil
@grebulocities8225
@grebulocities8225 Жыл бұрын
Fats/oils don't dissolve fully in ethanol at room temperature but do dissolve at the boiling point of ethanol. For this test to be accurate, the oil needs to be fully dissolved in the ethanol, so the ethanol is heated to its boiling point to ensure the oil is all dissolved.
@abetguerzon1939
@abetguerzon1939 3 жыл бұрын
from your test how can i measure the amount of insoluble matter in alcohol amd water
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
It is not the test method for insoluble matter in alcohol and water.
@hastesidagro-allied1676
@hastesidagro-allied1676 29 күн бұрын
What is the unit lf the final value gotten. Is it in percentage or mgKOH/g
@RathiVijnana
@RathiVijnana 5 ай бұрын
= 28.2* T.V * Normality NaOH/Weight of sample
@MicroChemsExperiments
@MicroChemsExperiments 4 ай бұрын
Yes. Correct
@SwhyBe
@SwhyBe 2 жыл бұрын
Great video still have question when I want to measure the acid value of candelilla wax it starts to solidified in the middle of titration ? How to solve this ? And will it affect 9n acid value?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Keep the flask on hot plate while titration and use magnetic stirrer to agitate the solution.
@atiqsaifi9615
@atiqsaifi9615 8 ай бұрын
Thanks from your good video. I have a question. Can we use acetic acid 33٪ instead of concentrated by adding three times more acetic acid ؟
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
You do not need acetic acid in this experiment
@AkashKumar-xp8hy
@AkashKumar-xp8hy 2 жыл бұрын
Thank you so much
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You are welcome
@gauravsinghgauravsingh6553
@gauravsinghgauravsingh6553 2 жыл бұрын
Very good bhai
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Thank you
@ujjwalkoirala6770
@ujjwalkoirala6770 5 ай бұрын
Only 2-3 drops of phenopthalein indicator is added what if we add 6-7drops.
@rashibhasan873
@rashibhasan873 3 жыл бұрын
Please check my procedure to help me whether i am right or wrong. I at first take 99% etanol and add it to 5 gm oil sample. Then heat it for 7-10 minutes and then add 1 ml 1% or 5% phenolphthalein indicators and carried out titration Against KOH. Then a blank titration is done. Volume of KOH = KOH required for the test - KOH for blank. Say KOH for Sample = 0.8 KOH for blank = 0.2 Volume of KOH = 0.6 I then use the following formula ACV = Molarity of KOH * Volume of KOH * Molecular weight of KOH / sample weight. In this i Don't need to neutralise the alcohol as i carried out a blank experiment.
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Correct
@krantidippawar2254
@krantidippawar2254 Жыл бұрын
Good video, Pls unit for acidity is missing.
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
Acid value does not have any unit. It is a value without unit like pH.
@manzano_ja
@manzano_ja 3 ай бұрын
Hi, do you know if I could use Isopropanol instead of Ethanol?
@sonofasnitch2172
@sonofasnitch2172 3 жыл бұрын
good video btw 👍
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Thank you so much. Be with us.
@mohammedmousa9540
@mohammedmousa9540 3 жыл бұрын
Good video btw 🙂🙂🙂😀
@nhdmaheswari2561
@nhdmaheswari2561 9 ай бұрын
How to know whether the ethanol is dissolved or not because my solution always separated as ethanol and oil after i removed it from hot plate
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
No problem. During heating, acidic properties comes with ethanol.
@sonofasnitch2172
@sonofasnitch2172 3 жыл бұрын
or do we need to wait until the mixture is cooled down to room temp?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Son of a Snitch, you have to carry out titration when the misture is hot.
@sonofasnitch2172
@sonofasnitch2172 3 жыл бұрын
@@MicroChemsExperiments noted. thank you🙏🏻
@ImranAli-vd6pd
@ImranAli-vd6pd 2 жыл бұрын
Son of a snitch means?
@avinashmokashe527
@avinashmokashe527 2 жыл бұрын
Thanku so much ma'am 🔥✨❤️
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Most welcome 😊
@virengemini6392
@virengemini6392 Жыл бұрын
When the sample is prepared you are boiling it, but my experiment wants to see the effect of frying on acid value so is it possible to do it in a different way to prevent boiling of the sample?
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
No. Boiling is needed for melting, dissolving and mixing of oil with ethanol. Boiling does not change the acid value significantly
@jancy98
@jancy98 Жыл бұрын
Why don't you show the standardization of NaOH in this video?..isn't it necessary?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Yes. Standardization of NaOH is must. We didn't show the procedure of standardization in this video. We just mentioned that standardization is needed. We developed another video for the standardization of NaOH and already uploaded in this channel. Please find the video if needed. Thank you
@jancy98
@jancy98 Жыл бұрын
@@MicroChemsExperiments ❤️
@aicha98-ii7xc
@aicha98-ii7xc 10 ай бұрын
Please Ihave a question, why did you heat the ethanol with sample and what is the time required to heat
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
Boiling is needed for melting, dissolving and mixing of oil with ethanol. Boil for 1 min
@marchiter8811
@marchiter8811 3 жыл бұрын
Why do we put an equal amount of alcohol and ether when determining or performing for acid value?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
We use 50ml of neutralized ethanol to dissolve the oil sample because AOAC method said so
@chegufoods9425
@chegufoods9425 3 жыл бұрын
What is the maximum value of ffa for a good oil...?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Different oils have different FFA value. Search on google to get idea about the reference ffa value of an oil sample
@SwhyBe
@SwhyBe 7 ай бұрын
Good day is there unit for the acid value ? due he did not write it
@jolll2469
@jolll2469 2 жыл бұрын
Thanks a lot..but i have a question, this method is also applicable to virgin coconut oil
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Yes.
@kwesimensa6431
@kwesimensa6431 2 жыл бұрын
What is unit for acid value, and you didn't also show the calculation of free fatty acid
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Please find the corrected calculations with unit from pinned comment. Thank you
@SohelRana-ln7bg
@SohelRana-ln7bg 3 жыл бұрын
Thanks a lot, Pls peovide FFA method & It's calculation
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
This video is also applicable for FFA test. Just FFA calculation is different. FFA calculation is given in the description section of this video.
@prashantbhil
@prashantbhil 3 жыл бұрын
How to find expected Acid value ???
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Search on google, find from journals, international specifications and standards (Indian standard)
@vishnukanthful
@vishnukanthful 3 жыл бұрын
Whats the procedure for groundnut oil ?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Same procedure will be applicable for groundnut oil sample.
@medamine5787
@medamine5787 9 ай бұрын
thank you for this clarifying experiment, i have a question, you used 10 g of oil, bcs i don't have enough samples what if i used only 2 g of oil, do i need to divide all mixtures by 5? Please reply me
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
g is not enough for the analysis. Do the experiment using 10g.
@nimishabaiju7393
@nimishabaiju7393 2 жыл бұрын
Thank you
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You are welcome
@arslanmunir9334
@arslanmunir9334 Жыл бұрын
Can you please provide a documented file or article of the procedure ?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Sorry. We don't provide any document.
@oluwaseyiomotayo785
@oluwaseyiomotayo785 3 жыл бұрын
Thanks for the video. A question though,what about orange peel oil!? What procedure is useful?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
This method is applicable for all kinds of oil sample.
@oluwaseyiomotayo785
@oluwaseyiomotayo785 3 жыл бұрын
@@MicroChemsExperiments thank you. About the standardization, how is it performed please? The steps weren't mentioned here.
@osharivepaul8709
@osharivepaul8709 Жыл бұрын
I really love your easy methods. Please help with this question. Based on this method , my expected acid value is 15-75. Will it be right to use 0.5g of sample and 50 ml of ethanol?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Yes. Do it
@dropin409
@dropin409 9 ай бұрын
​@@MicroChemsExperiments FFA content in oil only or also content in dried sesame seeds?
@saeedasadi2325
@saeedasadi2325 11 ай бұрын
Hi. Im iranian chemist, why heat erlen before titration? Your erlen contain oil , it leads to increase acid value
@MicroChemsExperiments
@MicroChemsExperiments 10 ай бұрын
AOAC method describe in this way of heating
@user-eu6ff2dm9m
@user-eu6ff2dm9m 9 ай бұрын
hi is acid value depends upon the number of grams of fat taken. i take normally 1 gm of mustard oil to calculate ffa .what should be its range in that case
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
You should take 10g sample
@tee4052
@tee4052 Жыл бұрын
I did acid value today on hydrogenated vegetable oil as per USP method. United States pharmacopeia. They say to melt the sample (was in powder form) and add 100 ml of hot alcohol previously neutralized with NaOH, to phenolphthalein ts. Then add 1 ml of the indicator. How would you interpret this? Do I add the naoh to the alcohol first and then heat or do I add after heating? And the indicator after heating? Pls help. I think I messed up here somewhere so it didn't pass
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Do as done in this video
@sharmiyaa7056
@sharmiyaa7056 2 жыл бұрын
Thank you so much mam
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You are welcome
@Iqra-ix1cf
@Iqra-ix1cf 6 ай бұрын
Thnks ❤
@hind1245
@hind1245 3 жыл бұрын
Thanks so much for sharing, we dissolve sample with solvent may be (hot ethanol) or a mixture of 96%ethanol and diethyether(1:1)...and heating the sample with solvents if necessary by gentl heating about 40°c.. Because high temperature destroy triglyceride and realise free fatty acid ..
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
We didn't try this method you described. Please try our method described in our video which was made by following the AOAC strictly.
@IrfanAli-xy5bf
@IrfanAli-xy5bf 3 жыл бұрын
Miss why are you adding phenopthline for twice?. Also requested share procedure for rancidity test with reference. Thanks.
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Irfan Ali, Thanks for your question. Yes adding Phenolphthalein twice is needed for this test. First time Phenolphthalein is added to carry out the neutralizing titration. Second time, it is added for the Acid Value determination titration. Both are needed because during boiling Phenolphthalein activity is lost in heat. So you have to add Phenolphthalein again before starting the Second & Final titration. We will try to upload a video for rancidity test very soon. Stay with us.
@killjadeskye
@killjadeskye 2 жыл бұрын
Hi, does the mixture have to be heated until boil? if so, how long should i let it boil?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Boil until the oil is dissolved in ethanol completely
@ujjwalkoirala6770
@ujjwalkoirala6770 5 ай бұрын
Can we use 0.5%phenopthalein indicator??For av determination.
@shyamgl9
@shyamgl9 10 ай бұрын
Sir i have a question . Is there any machine available to check moisture for ghee?like the BR check machine .
@MicroChemsExperiments
@MicroChemsExperiments 10 ай бұрын
You can use general moisture analyzer
@oldisgold5197
@oldisgold5197 Жыл бұрын
Can we use methylene blue instead of phenolphthalein? Then what are the procedures?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
No. You can't
@hhasheminejad1502
@hhasheminejad1502 2 жыл бұрын
Thanks a lot for sharing this very informative video. I have a question, can I replace EtOH 96% or 70% instead of 99%?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You can use 96% but not 70%
@udaygoud1017
@udaygoud1017 Жыл бұрын
Can you explain why you perform this test
@dayooshilaru9313
@dayooshilaru9313 Жыл бұрын
Thank you for this video. I want to ask, if we use KOH as the titrant, definitely the formula will will take molecular weight of KOH?
@grebulocities8225
@grebulocities8225 Жыл бұрын
Some measures of free fatty acids use KOH as the standard while others use NaOH. To convert from KOH to NaOH, multiply by 40/56.11. Likewise, multiply by 56.11/40 for the opposite conversion. This ratio is just the molecular weight of NaOH to KOH.
@grebulocities8225
@grebulocities8225 Жыл бұрын
To be clear, the AOAC method uses KOH. If you're titrating with KOH, just use the KOH MW (56.11) instead of 40, and you'll have the right acid value according to the AOAC method.
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Yes, absolutely
@23darshana
@23darshana Жыл бұрын
The calculation in description section is wrong! 56.1 is the molecular weight of KOH and since you've used NaOH so it should be 40.001. So the actual calculation should be Av= (40.001*normality of NaOH*volume of NaOH)/ Weight of the sample FFA(%oleic acid) = (28.2*Volume of NaOH*normality of NaOH)/weight
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Please recheck your justification
@MrLongKR
@MrLongKR 5 ай бұрын
Hi. Where can we get the official copy of this method? is it available online?
@MicroChemsExperiments
@MicroChemsExperiments 5 ай бұрын
Not available at online. You can purchase from us. Write us at mail.mic.chem@gmail.com to purchase
@blackbirdchannel9511
@blackbirdchannel9511 Жыл бұрын
Pls make a video on tannin in tea
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
Noted
@ayeshanajam7001
@ayeshanajam7001 Жыл бұрын
After neutralizing ethanol just add to sample and titrate Why boiling??
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
For melting, dissolving and mixing of oil with ethanol
@fransiskarusli439
@fransiskarusli439 3 жыл бұрын
hi, how do we know the "expected acid value" if let's say I do not know the profile of the oil I am about to test?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
If you dont have any reference expected value then test your sample twice or thrice asuming high, medium & low acid value. After performing the three tests you will get an idea about the acid value of your sample.
@ayeshabano1739
@ayeshabano1739 Жыл бұрын
Kindly mention the proper reference of this recipe. Or any pdf or paper u have followed.
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Reference is already mentioned in the title. No pdf is available to share
@abigailabu9299
@abigailabu9299 5 ай бұрын
Please help me out, how do calculate FFA from acid value dealing with shea butter sample
@MicroChemsExperiments
@MicroChemsExperiments 5 ай бұрын
Follow the calculation given in the description section of this video
@abigailabu9299
@abigailabu9299 2 ай бұрын
What you calculated in this video is acid value, is it the same with free acid value
@irawansukma7540
@irawansukma7540 3 жыл бұрын
How to calculate the acid number of a mixture of oil? If i want the total mixture of oil is 10 gram and i have 2 oil with diffent acid number. One is 0,1 and the other 4. How much of each of these oils? Thank you
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Sorry we are unable to explain for oil mixture.
@m.sulimannessary2885
@m.sulimannessary2885 Жыл бұрын
Please tell me that the determination of Acid value with FFA is different?
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
Please read the description section
@fazlanmohomed7756
@fazlanmohomed7756 Жыл бұрын
In our laboratory we are using methanol instead of ethanol, but the results are nearly same, is it possible?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
You should use ethanol
@AmitBiswas-ed3hy
@AmitBiswas-ed3hy 2 жыл бұрын
Thanks
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You are welcome
@tusharpanchanan5074
@tusharpanchanan5074 2 жыл бұрын
Kindly told me What is the procedure to Determine Free Acid, Total Phosphorus Pentoxide and Water Soluble Phosphorus Pentoxide Presents in Single Super Phosphate ??? It's Urgent
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Noted. We will develop video later
@munishtiwari4097
@munishtiwari4097 Жыл бұрын
How to find out the free fatty acids value of rice bran oil
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Same method
@munishtiwari4097
@munishtiwari4097 Жыл бұрын
Which indicator is to be used
@olasupooladirantayo9711
@olasupooladirantayo9711 Жыл бұрын
Please what is the implication of Acid value on oil quality?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
The higher the acid value and free fatty acid content, the lower the quality of the oil
@rihab.jelassi.nutritionniste
@rihab.jelassi.nutritionniste 2 жыл бұрын
Hello, is this protocol available for measuring AV of ghee ( butter oil )? and can you mention the link of the AOAC please? Thank you
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You can measure the acid value of ghee by this same method.
@lalitverma5279
@lalitverma5279 Жыл бұрын
But the molecular weight of naoh is nearly 40 so why we use 56.1 for acid value as you mentioned
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
You have to use 56.1 for both of NaOH and KOH
@lalitverma5279
@lalitverma5279 Жыл бұрын
It will be same for rice bran oil??
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
@@lalitverma5279 yes
@shilpak753
@shilpak753 Жыл бұрын
How to do solid sample like mixes for free fatty acid procedure tell me
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
Take 10g of crushed and mixed sample and do the same as done in this video.
@Haqoursuch-dg8wl
@Haqoursuch-dg8wl 9 ай бұрын
Why added ethanol in sample
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
AOAC method said so
@AshishKumar-rf5tv
@AshishKumar-rf5tv 2 жыл бұрын
What was the actual temperature of the Hot Stirrer during heating the sample with the neutralizing Ethanol? Thanks for your prompt feedback.
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You need to heat just at the boiling point of the ethanol. May be at 80 degree Celsius.
@AshishKumar-rf5tv
@AshishKumar-rf5tv 2 жыл бұрын
@@MicroChemsExperiments thank you very much!!!
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
@@AshishKumar-rf5tv you are welcome Sir
@balkishan9253
@balkishan9253 2 жыл бұрын
Mam please upload full video of find acidity as (oelic acid)
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Noted
@Mg01012
@Mg01012 Жыл бұрын
Dissolve 1gram phenolphthalein indicator powder in 100 ml ethanol? Am I right
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Yes right
@user-rh4rl9mm6v
@user-rh4rl9mm6v 6 ай бұрын
Can use this method for detect FFA in milk or milk product?
@MicroChemsExperiments
@MicroChemsExperiments 6 ай бұрын
Yes. You can
@azaruameer3931
@azaruameer3931 2 жыл бұрын
Can you give me the "Determination of Oil Analysis" manual if I need it?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
No sir. No documents will be provided
@kktv2181
@kktv2181 Жыл бұрын
Hi dear i have a question. all oil or dalda cooking oil what is calculation
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Same calculation
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