The aging reversal cocktail is: 1. 0.015 mg of HGH 2. 50 mg of DHEA 3. 500 mg of Metformin 4. 3000 iu of Vit D 5. 50 mg of Zinc

Notes: 1. [Amendment] I should have mentioned this (a mistake on my part), but the study was not a double blind, randomized controlled trial. It was an intervention trial, however it did not have a control group. I still believe the results of the study, but that belief is not grounded on any foundation of evidence beside the fact that other studies have shown effects of these molecules in other trials. So, up to you if you believe the results to be non-placebo driven. My apologies for not adding it - it completely failed me, and it’s important information. 2. People have been confused on the metformin part - the idea in this study isn’t to use metformin for its life extension properties, but to maintain insulin sensitivity. I’ll look at metformin independently (and berberine) in the future. 3. DHEA was added to control insulin release. So, both metformin and DHEA were added to mitigate potential negative effects of HGH alone.

A great example of how superb Nicolas is when translating rather complex science to engaging and useful content. Thank you!

12:38 1. HGH 2. DHEA 3. METFORMIN 4. Vit D 5. Zinc

Would be great to see separate videos describing how all these 5 components are working inside our bodies.

Congrats on 100K, Nicolas! Proud of ya, man. That’s awesome and very well-deserved! 👏👏🥳🎉

I am in a study. I’ve been in it for over 40 years now. It involves exercising 3-4 days a week, conscious healthy diet, low alcohol intake, no smoking, have a career working inside, maintain healthy body weight throughout life, partake in hobbies that make you feel good, avoid drama. I’m almost 60 and I weight same as high school…the clothes still fit me. I have a few friends who say I sleep in a freezer. The muscle mass I’ve gained above baseline since a teen makes all the difference I think. I keep my muscles (every body part) working and in turn they demand fuel. This fuel is of course is the little body fat I have and other toxins that build up in the body. The turnover of fat in the body and increased blood perfusion to the entire body keeps the aging process at bay. I’m convinced of my personal “study.”

Just a week ago youtube suggested Greg Fahy and his trim-x study and now it's all here with details we love physionics for. Amazing!

Fantastic study!! I will have to share this with my friend who is interested in reversing thymic aging. Thanks for the study. 😃 To boost HGH naturally, all you need to do is lose body fat, decrease insulin levels (which is why they likely used metformin), increase arginine, lysine (unless you have cancer or pre-CA lesions), get enough sleep, HIIT exercise (anaerobic exercise that stimulates HGH by up to 450% during the 24 hours after you finish your intense fast workout.), resistance exercises in a load-dependent manner (aerobic exercise), and do more intermittent fasting (those who do 24-48h can increase HGH 3 to 5-fold. But do at least 12hrs if you want to see a bit of a raise, 18-20h is optimal for those who like doing it daily). DHEA is useful in old age due to lower levels. My friend takes pregnenolone instead which is the master precursor hormone of all your hormones. I am not taking of these yet till I get older. Right now don't need it. Berberine may be used in place of metformin because it will decrease both insulin levels + triglycerides + lipid levels in a safer natural way, besides activating AMPK. D and Zn are a no brainer. Thanks again for this cool video!! :D

GH-->IGF1-->'new' skeletal muscle and bone growth (reflected to a degree by increased serum ALP level); there's your 'connection'...you're welcome!

Your analysis of these studies is soooo helpful and appreciated! Your well researched work is incredibly well done. On the study molecules…I was on Metformin by request as I’m close to being pre diabetic, but have changed to Berberine to minimize the impact that Metformin has on VO2 max (workout) reductions and the effect it has on reducing testosterone. Berberine has helped reduce my A1c and lipid readings. As a 68 yr old male, I really want to keep my workouts to be as effective as possible and no male wants testosterone reduced by medication.

I'm a new subscriber and I love your simple but informed communication. Thanks for covering these stories on health and wellness with detail. ❤

Thank you for the video Physionic! I learn so much from your channel

I think the key molecules are Vitamin D3 (should be taken together with Vitamin K2) and Zinc (3 types). The zinc is a well known Thymus gland enhancer

Thank you for covering this!!! eagerly interested to see your next video on it.

As a 58 year old, now understanding more about keto/carnivore, and the benefits it has given me in the last 6 months, I would love to be involved in trials. Experimenting on myself and doing analysis has been a really good and positive experience.

Nice one! The protocol seems realistically feasible. Excited for the data of phase 2!

Interesting. For me, this information is really important because I am aiming to find a way to stop or prolong aging. Thanks a lot for this video, it’s easy to understand and you didn’t skip any major details while focusing on the main point of the topic.

Extended fasting over 48hrs dramatically increases HGH, and I have been taking 25mg DHEA for 5yrs now (I’m in my mid-40’s), along with Berberine in place of Metformin. Great results from my own side, this study merely reinforced my stack and protocol was potentially on the right track to have a positive impact on aging. I recall when this came out at the time, and was surprised to see DHEA in the trial, but it nonetheless left some quite interesting epigenetic results - I just take all of those clocks with a huge pinch of salt.

what you do is so precious. I could not reach this knowledge without you translating it for me. My brain can't handle complicated stuff. You make transform information into bearable to understand.

I was hoping the stack was a triple decker peanut butter and banana sandwich.

Fascinating! In my mid to late 20’s I was a participant in the human growth hormone study and have since taken three of the other four compounds (excluding metformin) used in this cocktail at various times.

Your dry humor is spot on!

If you want budget fixes, they are as follows. 1 red light therapy for hgh. 2 apeginen for cd38 reduction. 3 Berberine instead of metfomin. You could always sun blast yourself for vitamin d. Happy living!

Hello! KZfaq recommended this video to me, and I'm glad it did. You do a superb job of explaining the results of the study. Definitely coming back to this channel.

Thank you very much! I really appreciate your work, Nicolas!

Great info thank you Nick.

Bloody fascinating!

So, that's what I found out so far and why you probably see this "coincidently result". - Epigenetic clocks are sometimes based on "whole blood". - Many times are using something like a "Random forest regression". - The problem of whole blood is that you take a mix of cells, including B and T cells, and make an average of the epigenetics. - Now let's take for example the methylation of cg02162324. - in a sample I have here, the methylation of B cell for this CpG is 0.25 where the methylation of T cell for the same CpG is 0.61 in the same sample from the same patient. - As we age, the proportion of T and B cells change, and probably that's what's happen on this research when they start to play with the immune system. - So when you apply an aging clock like horvath clock trained on whole blood, you are possibly (at least is the conclusion I'm arriving on my study), not calculating the age, you are calculating the proportion of B and T cells and therefore estimating the age. - So although multi tissue ageing clock are great for a lot of things, I believe that for epigenetic ageing must be calculated using single cell methylation.

You can take peptide bio regulators to regrow the thymus, pineal gland etc

You are such a tease! Never change tho! Thanks for the info, luv your channel.

What I find interesting about the stack used is that it didn't appear to include any of the popular and more widely promoted anti-aging supplements like NMN, GlyNAC etc and also the likes of omega3, collagen etc.

I just love your videos! So professionally done in every manner way shape and form! I, myself, try to retard the aging process by eating a (mostly whole organic) plant based diet, with lots of antioxidants, with just 1 organic pasture raised egg daily. I do also take organic nutritional supplements. At 75, I still mountain bike, hike, swim, etc. Most people thing I'm around 59 or 60.

I started and read Sinclair book,love the concepts, I use some of the Ideas , first year , my blood work was , perfect , my cardiologist, was blown away ...I was 70, now 75 my strength, is real good , I ride bike 25 miles a week , and weight lift , I take NMN , nac, tmg,,,,, some others too...

Very interesting 👏 Thanks for sharing your research journey 🙏

My secret to reverse aging. I drink from the cup of the Holy Grail. I chose wisely.

Love your videos (and humor)

Thank you for this wonderful piece, Nic.

Thank you for this type of info!!

I keep looking at that spider on the wall

Very intriguing topic. Like some other commenters, I was surprised at the zinc dosing. It also raises questions about whether the mixture of molecules should change over time and based on other medical conditions.

Really great presentation. Thank you.

Started Thymus Gland extract a couple months ago and at 41 I noticed a difference in my vitality. But excellent presentation of information, definitely sharing this with my clients who comment on my youthfulness. Most say I look 30 or younger…

ALP levels can be elevated during periods of rapid growth, such as childhood or adolescence, due to increased bone remodeling so this rejuvenation stack could be improving bones.

Cool job my friend

This was very helpful. Thank you.

Thank you for your objective exposition.

very nice. The fact that the thymus density increased is certainly something. Personally I would love to see a similar study but complemented with Thyroid, gonadal and adrenal hormones. Not just growth hormone, dhea and vit D. All hormones are abruptly down after 50. The production stops, the glands are old. Doesn't matter what you do to stimulate "naturally". Why not supplement everything that is available ? Sterling work Nicolas, as always. Thanks !

I think the simplest way a person at home can measure if their age is reversing, given that they already have visible signs of aging, is when their gray hair regains its color, when their wrinkles start to disappear, when their skin begins to become more taut, when their thinning hair begins to thicken, and when their receding hairline begins to fill in

Worth keeping up with. Thank you.

Thank you for putting in your time and effort into making these great videos. Your work is much appreciated!!! ALKALINE PHOSPHATASE (ALP) plays a pivotal role in the process of bone mineralization. It hydrolyzes inorganic pyrophosphate, a potent inhibitor of mineralization, to produce inorganic phosphate. This inorganic phosphate combines with calcium to form hydroxyapatite crystals, which are the main mineral component of bone. By breaking down inorganic pyrophosphate, ALP facilitates the deposition of calcium and phosphate into the bone matrix. I would love to see if there were any changes in bone density.

I think the way you put things across is a gift & really easy to understand. It's a pleasure to listen too :-)

Increased HGH can come from exercise and/or fasting. DHEA, D, and Zinc can come from easily available supplements. Metformin isn't easy for non-diabetics to get, but Berberine is easily available, has some similar effects, and may work anywhere from less well in this concoction to better than the Metformin. It seems likely that the measured antiaging effects seen in this study are the simple sum total of the effects of the individual substances rather than some 'magical' interaction between them. Anyone else have thoughts on this?

metformin is raising the ALP, an alternative to metformin would be taurine supplementation.

You are just fantastic! Thank you.

Interesting review, thanks for sharing. My question is once the reversal is achieved is it locked in or like most things has to be consistently maintained?

Do you think it would be feasible to have a conversation/friendly debate with Dr Anthony Chaffee? he seems to be doing podcasts/interviews everyday but most the people he speaks to arent well versed in the medical literature or already have their mind made on carnivore etc. Would love to see you discuss the topic with him as I really think he is the most eloquent and coherent speaker on this.

I love how you sustain the suspense 😊

Nice to see the channel taking off, never thought there would be so many nerds on KZfaq. In the end, science will prevail!

There was another study done in a university somewhere in the NW United States (sorry, I can't remember the one) that was working on an experiment with NMN and NAD. The idea was to check the cell maintenance processes, and they had an amazing response as well that apparently reversed several aging metrics. Are you familiar with this study? This would have been a few years ago.

Thanks for posting.A few questions - how often was the stack given? Why did they chose that particular combo? I didn’t see a link to the study, were there similar studies that helped them hone in on this combo. I’m thinking the growth hormone alone would have the greates effect.

I absolutely love every time he says glomerular filtration rate ! ❤ I’m a dialysis nurse so makes me smile to hear that struggle. So hilarious. Love this guy

You are such a skillful tease. I must be a masochist, because I enjoy it. Science is very interesting, but you turn-on the fun part, without distracting from the information flow.

I was curious, with what frequency was the cocktail administered? Thank you for the video.

I’m pretty much using this stack now. Good thing I’m a somewhat older man. Losing weight and exercise combined with fasting for the HGH, Berberine in place of Metformin, Vit D, zinc, DHEA all easy to get without RX. You mentioned the rise in Alkaline Phosphatase . For me has always been an elusive marker- could be taking Vit D without K2 could monkey with the calcium blood levels and in turn push AP all over the place. Subclinical - parathyroiditis messed with my calcium & AP for most of my life. PSA is another marker that is easy to push around- Salmon oil - mine is always under 1.0. Good stuff- thanks for the news. 1:16

Great video!

I'm a thrilled new subsciber ! This was the channel I've been searching for, thank you for your brilliant work and for sharing it . I am not well, struggling against it and doing that alone, your information will probably save my life. I mean that , with gratitude. Bahia

My grandfather lived to 104, never took a supplement in his life. He was an MD and advised people to eat a wide variety of wholesome foods, but not too much. Sounds a lot like Michael Pollan.

That's very cool, so when and where can i buy this cocktail 😂?

You are the very best, you made me love science. ❤️🙏🙋🏼‍♂️🇨🇦

Thanks for sharing this interesting and beneficial information to us.

The most interesting thing about all of this data you show, but that you never comment on is that the positive effects seem to reverse around the 10-12 month mark in every case.

The immune modulation and thymus restoration was probably entirely due to HGH inducing IGF-1. The metformin was added to reduce insulin secretion due to HGH, because DHEA could not do it. The increase of the liver enzyme is most probably due to the high dose of zinc - probably toxic over long term. Epigenetic clocks correlate with aging, but that doesn't mean that reducing methylation translates in actual age reversal - Bryan Johnson has an epigenetic age of 20 years old, yet doesn't look like it.

Thank you!

I am a woman. I take all of this except HGH and I take 25 mg. Of DHEA and 50 mg. Pregnenolone. I take 25 mg. of Zinc.3000 Vitamin D Seems to work! I am 65 and people think I am 55.... thank you for this!

Very interesting. I am a fanatic about health. Up until 68 I didn't think much about it. I don't drink or smoke but I did eat mainly fast food. Until 68 I ran 5k and exercised every day.Then arthritis set into my knees, wrists and back. I had to stop running and exercising I gained weight and became a couch potato and KZfaq junket. From KZfaq I learned about Keto the results were so amazing I quickly migrated to Carnivore and for the last 4 years all I eat are animal products. I'm now almost 77 and healthier than when I was at 65. Thank you so much for your information on the age reversal study. Tomorrow I will be ordering HGH, ,Megformin and DHEA. I already take Vitamin D3 and Zink. I am looking forward to 120.😅

glad i found your site.. dealing with doctors and trying to self educate is a shameful dispute that should never take place,, but it does sadly

In putting the idea to practical use it is amazing to be able to simply exercise and eat better to relieve pain, increase mobility and have a better quality of life. I can run again for the first time in years.

Just to clarify, that HGH dose of 0.015 mg/kg was done 3-4x per week and not daily? From what I recall reading the study when it first came out.

Metformin usage always makes me raise an eyebrow: it's known for its reduction in mythocondrial function, that to me seems a big no, at every age.

Very interesting, entertaining and informative. Thank you.

Very interesting and I really want to follow up next year with the Phase 2 study. Curious though: I noticed the graphs almost all show increases (or decreases depending how you look at it)…reversals in the improvements. Does this indicate the participants may have to continue taking the cocktail, or increase the portions, or does it stop working after about 12 months? Thank you, and as interesting as you made this, I subscribed!

Very interesting…so glad you shared this with us, I wonder if you have done a research study on senescent cells?

It would be interesting to know the participants initial vitamin D levels and Zinc levels, since I hear most people are deficient. However one wouldn’t really expect any benefit unless one was initially deficient.

Really interesting as all your videos always are. Would appreciate your take on Bovine Leukemia Virus (BLV) and cancers

Thank you for this, Nic! I'm already taking 3/5s of this, zinc, vit. D, & DHEA. I also take berberine, a 'natural' version of metformin. Some of the supplements I take are thought to be HGH 'boosters' as well. I started taking supplements because testing showed some deficiencies. Because I was diagnosed w/ asbestosis & COPD steroids were prescribed. They have a tendency to produce hormonal imbalances that cause further health issues. Including nutritional deficiencies. But the supplements are helping just as the Intervene Immune stack has shown. Keep up the good work, & ; Have a GREAT day, Neighbor!

Man, I would love to see a study where they drop the metformin and increase the vitamin d to 30000 IU, and add some good sunlight exposure, and a few cold shocks. Are so hard to do without extremely technical lab equipment. Just discover your channel, excellent information and by far the best explanation I've seen yet that was short, simple and sweet about epigenetics! Subscribe and thank you!

Excellent content!

You're such a tease! 😎 I was so waiting for the dosage and supplements. Thanks buddy! By the way the 500 of Metformin really surprised me.

I'm the only messenger My 5 are N-acetyl cysteine Vitamin D Glycene Taurine Fish oil I feel so good as well

Could you please provide comment on the potential efficacy of Berberine vs Metformin, as it relates to this application?

0:21: 🧪 A study conducted at Stanford Medical School showed promising results in reversing aging in a small group of men. 3:27: 🧪 The researchers investigated the effects of time-restricted feeding on immune health and found that blood insulin and glucose levels did not change, but they were more interested in thymic involution. 5:27: ✅ Intervention increases thymic fat-free fraction in most participants, but not all. 12:07: 🔬 The study found that people who experienced a six and a half year reversal of epigenetic age also experienced improvements in several indices of clinical health. 8:42: 🧬 Epigenetic clocks are a way to measure and track aging based on changes in epigenetic tags on our genes. Recap by Tammy AI

I will just comment a very well thought out and scientifically sound, "wow!" -- Your videos are awesome.

Good luck getting HGH legally. It took 2 yrs to find a competent endocrinologist willing to screen me. He measured a large number of hormones, many labs, a 24 hour urine, and performed adrenal function IV stimulation test, as well as a several hour long IV GH stim test. Poor GH results began the “application” process. Including a full (rule out) cancer screening (colonoscopy, mammography etc) and body composition/bone density etc etc. Results are submitted for “approval”. HGH is more expensive than many people’s house payments. Which began another process - submitting my 1040s tax forms to be approved for financial assistance. HGH treatment was extremely beneficial on the short term. But it’s no panacea - adverse (painful) side effects had me beginning to wean within a year- until I stopped taking it altogether in 2 yrs. That’s the reality. The need for HGH treatment is NOT for the faint hearted...

I heard that 10-20 zinc is enough from another study. Great video as always!

I just read the article last night where Peter Attia who is writing about the particulars about this TRIIM study (Thymus Regeneration Immunorestoration and Insulin Mitigation) mentioned it was a weak study because there were 9 or 10 people (the article says 9, the study shows 10) only men (as NV laments during a podcast) and there was no control group. They added the DHEA because the HGH increases cholesterol and the main author of the study, Fahy, added it in based on a hunch- he was right- the cholesterol went back down on the DHEA. Thymus seems very important as the study cites roughly at about age 63 is when cancer, infectious disease, autoimmune conditions, generalized inflammation, arthrosclerosis and all cause mortality increase due to lack of T cell production which happens in the thymus gland as well as lack of t cell receptors. As @physionic points out in his recent podcast, adipose tissue collected around the thymus gland basically kills it off and removing the fat enables it to function again. This was shown in diagrams of the gland in the study! So the conclusion was to mitigate insulin production, and restore immune function by regenerating the thymus gland. (Sorry, I'm very good at stating the obvious.) Regarding the epigenetic effect mentioned, it only gained about 2 and 1/2 years. I'm wondering how that can be true because earlier in the study they mentioned that the immune response in centenarians is still apparent and this was part of their goal. Maybe if we could see a comparison with the blood markers of a centenarian we could measure the effect more accurately. So the goal was to keep the vibrancy of the immune function in order to prolong life. If this is so, why not also prescribe thymosin alpha 1 to everyone? There are no poor side effects. This has the effect of increasing hematopoiesis which is the goal here. Keep everyone strong, apo B and LDL low, a nice low body fat percentage (BMI can be higher because of muscle weight) and take higher amounts of B vitamins for the NAD effect, especially B12 for higher RBC count? I think that would solve many of the problems along with keeping low insulin- very significant because it was perceived to contribute to depositing fat, which needs to be controlled to liberate the thymus gland so it can do its job. A few things I noticed about the study is that exercise is not mentioned at all. The weight of the men or weight change is not mentioned. And diet is not mentioned at all. With no control group and only measuring the blood markers, I am wondering what the real significance is of a study like this could be without other measurements and records. Interesting study but I'm sniffing stacks as opposed to lifestyle changes. I think the focus should be on the function of the thymus gland, and what we need to optimize the whole body response to that which is much broader, and not the stack.

Good one. In the very recent past, i'm finding that having zero nuts in my diet lowered for this person nearing 70 from gut barrier leakages (rashes going away) which in turn possibly lowered the immune system becoming hyperactive (not measured) Have no idea if this "demand side" benefit can act in parallel to the "supply side" improvement observed in the TRIIM study. eGFR improvement is my goal as well.

I couldn't even fathom the wealth a company could acquire if they were able to stop or reverse aging.

I used metformin for a half year or so. My work out results nose dived to negative results, and I began having cramps in muscles that had never cramped before, like incredibly painful diaphragm cramping that was so bad I thought it was a hernia. Stopped metformin -- work out result returned to normal, cramping decreased significantly. In my seventies I question if the results are more positive than the loss of muscle mass production is negative. Addendum: When investigating the cramping was told by a very experienced nurse that the cramping indicated that I did not need metformin. Which I knew, but I think it did indicate that I was already insulin stable. This may be due to continual benign twitching of muscles in my legs for decades. This will keep blood sugar stable, strangely enough.

Another fascinating explanation. Do you ever plan to deep dive into bone and joint health? Thank you.